Immune gene expression in rainbow trout experimentally infected with Anisakis simplex larvae Haarder, Simon; Kania, Per Walter; Buchmann, Kurt Publication date: 2012 Citation for published version (APA): Haarder, S., Kania, P. W., & Buchmann, K. (2012). Immune gene expression in rainbow trout experimentally infected with Anisakis simplex larvae. Abstract from DAFINET: Immune Responses in Fish, Frederiksberg, Denmark. Download date: 27. Sep. 2021 DAFINET WORKSHOP IMMUNE RESPONSES IN FISH November 6th and 7th , 2012 Venue: Organised by: Lecture Theatre 1-01 Danish Fish Immunology Research Bülowsvej 17 Centre and Network 1870 Frederiksberg www.dafinet.dk Denmark University of Copenhagen Frederiksberg Denmark Book of abstracts DAFINET November 2012 University of Copenhagen DAFINET is supported by the Danish Council for Strategic Research The book of abstracts is edited by Per W. Kania and Kurt Buchmann Illustrations by Kurt Buchmann Printed by Frederiksberg Bogtrykkeri 2012 Frederiksberg, Denmark DAFINET WORKSHOP Danish Fish Immunology Research Centre and Network IMMUNE RESPONSES IN FISH November 6 th and 7 th , 2012 Venue: Organised by: Lecture Theatre 1-01 Danish Fish Immunology Research Bülowsvej 17 Centre and Network 1870 Frederiksberg www.dafinet.dk Denmark University of Copenhagen Frederiksberg Denmark Program Tuesday November 6 th , 2012 10.00 DAFINET Board meeting 10.00-12.00. Only for board members Scientific program 13.00 Welcome address by DAFINET leader Kurt Buchmann Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark 13.15 Research director Scott LaPatra Clear Springs Foods, Inc., Research Division, Buhl, Idaho, USA Immunization of rainbow trout against flavobacteriosis 13:45 Professor Barbara Nowak National Centre for Marine Conservation and Resource Sustainability, AMC, University of Tasmania, Australia Yersiniosis vaccine research update 14.15 Post doc Jiwan Chettri Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark Immune response of rainbow trout juveniles to the protozoan parasite Ichthyobodo necator : immunohistochemical and gene expression studies 14.45 Coffee break 15.30 Professor Barbara Nowak National Centre for Marine Conservation and Resource Sustainability, AMC, University of Tasmania, Australia Immune response of Southern Bluefin Tuna 16.00 M. Sc. student Simon Haarder Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark Immune gene expression in rainbow trout experimentally infected with Anisakis simplex larvae 16.30 Senior research scientist Bertrand Collet Marine Laboratory, Ellis Building, Aberdeen, UK Development of a non-lethal sampling method to monitor immune response and disease progression in salmonid fish 17:00 Wrap up by DAFINET leader Kurt Buchmann 18.00 Dinner at Stigbøjlen 7, building 1-35, Frederiksberg Campus Program Wednesday November 7 th , 2012 10.00 DAFINET leader Kurt Buchmann: Welcome to the second day of the workshop 10.10 Professor Barbara Nowak National Centre for Marine Conservation and Resource Sustainability, AMC, University of Tasmania, Australia Amoebic Gill Disease – development of new management strategies 10.40 Ph.D. student Sidhartha Desmukh Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark Characterization of central cellular and humoral mechanisms responsible for protection of vaccinated rainbow trout against Yersinia ruckeri infection by the use of histology and immunohistochemistry 11.00 Ph.D. student Bartolomeo Gorgoglione Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, UK A strong antiviral response is elicited by viral hemorrhagic septicemia virus infection in brown trout 11.20 Ph.D. student Melanie Andrews Norwegian School of Veterinary Sciences, Oslo, Norway Wound closure and initial healing processes following adipose fin clipping of Atlantic salmon 11.40 Post doc Maki Ohtani Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark Antiviral activities of pattern recognition receptors, LGP2 and MDA5 in Japanese flounder, Paralicthys olivaceus 12.00 Lunch at Stigbøjlen 7, building 1-35 Program Wednesday November 7 th , 2012 13.00 Ph.D. student Maya Henriksen Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark The role of the gills as potential portal of entry in rainbow trout fry syndrome 13.20 Ellen Lorenzen Department of Poultry, Fish and Fur Animals; National Veterinary Institute, Technical of University Denmark Improved protection of rainbow trout against furunculosis by an autologous vaccine under experimental conditions 14.40 Post doc Milena Monte Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen, UK Identification and expression analysis of four cytokine receptor family B (CRFB) members in rainbow trout ( Oncorhynchus mykiss ) 14.00 Coffee break 14.40 Ph.D. student Umaporn Khimmakthong Department of Molecular Biotechnology and Bioinformatics, Prince of Songkla University, Hat Yai, Thailand Immunization of pacific white shrimp by phagocytosis activating protein gene 15.00 Research assistant Rezkar Jafaar Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark Gut microbiota changes in rainbow trout, Oncorhynchus mykiss (Walbaum) during organic acid feed supplementation and Yersinia ruckeri infection 15.20 Ph.D. student Anna Shönhertz Center for Quantitative Genetics and Genomics , Department of Molecular Biology and Genetics, Aarhus University, Denmark Inter-species transmission of viral haemorrhagic septicaemia virus between turbot (Scophthalmus maximus) and rainbow trout ( Oncorhynchus mykiss) 15.40 Final discussion and conclusions of the workshop Abstracts IMMUNIZATION OF RAINBOW TROUT AGAINST FLAVOBACTERIOSIS Scott E. LaPatra Clear Springs Foods, Inc., Research Division, Buhl, Idaho 83316, USA Flavobacteriosis is primarily caused by Flavobacterium psychrophilum and F. columnare . Together they are the major bacterial pathogen contributors to losses in the rainbow trout ( Oncorhynchus mykiss Walbaum) farming industry in Idaho, USA and freshwater salmonid farming around the world. Outbreaks of Flavobacteriosis can result in significant mortality but further losses due to deformities and reduced growth in survivors contributes to additional and potentially greater economic impacts for the farmer. Disease prevention is essential to the continued development of aquaculture around the world and remains a primary strategy for avoiding Flavobacteriosis, however vaccination is the most effective method of preventing or minimizing disease. Killed, attenuated, subunit and a variety of other vaccine formulations against Flavobacteriosis have been developed and tested over the years and despite a number of publications there are still no commercially available vaccine(s) for rainbow trout. One slightly different, alternative “vaccine” we have evaluated is an Enterobacter sp. surrogate vaccine/immunostimulant against F. psychrophilum . Originally developed as a probiotic it shows promise, at least for short term protection when delivered by intramuscular injection. Equally important to the development of Flavobacteriosis vaccines is the development of mass immunization strategies. An efficacious vaccine is only of use to producers if it can be delivered cost effectively to small fish. With this in mind, a rifampicin attenuated F. psychrophilum vaccine has been developed and field tests suggest it can be effective when delivered by immersion. Similarly a live attenuated F. columnare vaccine licensed for use in channel catfish is also being evaluated for use in rainbow trout. Presenting author: Scott E. LaPatra, [email protected] YERSINIOSIS VACCINE RESEARCH UPDATE Barbara Nowak National Centre for Marine Conservation and Resource Sustainability, AMC, University of Tasmania, Launceston, Tasmania, Australia Yersiniosis is a bacterial disease affecting Atlantic salmon in hatchery and after transfer to the sea. While there are commercial vaccines available, outbreaks still occur due to the presence of carriers. Using cDNA microarray we identified the expression of 6 genes in response to infection and 4 genes associated with the protective host response to yersiniosis. Analysis by real-time PCR confirmed that three immunologically relevant genes, namely a cathelicidin (47-fold) and a C-type lectin (19-fold) increased in response to yersiniosis. Including collagenase (17-fold increase), an important tissue remodelling and repair enzyme, these genes represent 3 of 6 non-protective and/or pathological responses to yersiniosis. Genes associated with the protective host response included an immunoglobulin gene and a selenoprotein that showed significant fold changes (15-fold increases each), highlighting the importance of antibody-mediated protection against yersiniosis. A transcriptional biosignature consisting of predominantly immune-relevant genes (14 up and 3 down- regulated) in the gills of Atlantic salmon after immersion vaccination and before bacterial challenge was identified. This biosignature may be used as a surrogate of protection and therefore as a predictor of vaccine success against yersiniosis. We have tested an effect of different