Poster A001 A003 Paenibacillus baekrokdamisoli sp., nov., Isolated from Soil of Lentibacillus kimchii sp. nov., an Extremely Halophilic Crater Lake Bacterium Isolated from Kimchi 1 2 1 1 1 Keun Chul Lee1, Kwang Kyu Kim1, Jong-Shik Kim2, Dae-Shin Kim3, Young Joon Oh , Hae-Won Lee , Seul Ki Lim , Min-Sung Kwon , Jieun Lee , 1 1 3 Suk-Hyung Ko3, Seung-Hoon Yang3, and Jung-Sook Lee1,4* Ja-Young Jang , Jong Hee Lee , Hae Woong Park , Seong Woon Roh4, and Hak-Jong Choi1* 1KCTC/KRIBB, 2GIMB, 3World Heritage and Mt. Hallasan Research Institute, 4UST 1Microbiology and Functionality Research Group, World Institute of Kimchi 2 A novel bacterial strain Back-11T was isolated from sediment soil of crater Hygienic Safety and Analysis Center, World Institute of Kimchi 3 lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain Back-11T Advanced Process Technology Research Group, World Institute of Kimchi 4 were Gram-staining-positive, motile, endospore-forming, rod-shaped and Biological Disaster Analysis Group, Korea Basic Science Institute oxidase- and catalase-positive. It contained anteiso-C as the major fatty 15:0 A Gram-stain positive, aerobic, non-motile and extremely halophilic bacterium, acids, menaquinone-7 (MK-7) as the predominant isoprenoid quinone, designated strain K9T, was isolated from kimchi. The strain was observed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine to be endospore-forming rod-shaped cells showing oxidase- and catalase- and four unidentified aminophospholipids as the main polar lipids, and positive reactions. Strain K9T was able to grow at 10.0–30.0% (w/v) NaCl meso-DAP as the diagnostic diamino acid in the cell-wall peptidoglycan. (optimum, 15.0–20.0%), pH 7.0–8.0 (optimum, pH 7.5) and 15–40°C (optimum, The DNA G+C content was 45.3 mol%. Phylogenetic analysis, based on 16S T T 30°C). The polar lipids of strain K9 consisted of phosphatidylglycerol, two rRNA gene sequencing, showed that strain Back-11 was most closely T unidentified phospholipids and an unidentified glycolipid. The predominant related to Paenibacillus taihuensis THMBG22 (95.5%) and fell into a clade isoprenoid quinone was menaquinone-7. The major cellular fatty acids in the genus Paenibacillus. On the basis of phylogenetic, chemotaxonomic T (>20% of the total) were anteisio-C and anteisio-C . The cell wall and phenotypic data, strain Back-11 represents a novel species in the 15:0 17:0 peptidoglycan of strain K9T was determined as meso-diaminopimelic acid. genus Paenibacillus, for which the name Paenibacillus baekrokdamisoli sp. T T T The G+C content of genomic DNA was 48.2 mol%. Phylogenetic analysis nov. is proposed, with strain Back-11 (= KCTC 33723 = CECT 8890 ) as the based on the 16S rRNA genes revealed that the isolated strain was most type strain. closely related to the Lentibacillus salinarum AHS-1T with a similarity of 96.63%. Based on its phenotypic, chemotaxonomic and phylogenetic data, strain K9T is considered to represent a novel species belonging to the genus Lentibacillus, for which the name Lentibacillus kimchii sp. nov. is proposed. The type strain is K9T (=KACC 15543T =JCM 17730T). [This research was supported by a grant from the World Institute of Kimchi funded by the Ministry of Science, ICT and Future Planning (KE1601-2), Republic of Korea.] A002 A004 Rhodanobacter aciditrophus sp. nov., an Acidophilic Study on the Mycelium and Morphological Characteristic of Bacterium Isolated from Mine Wastewater Naematoloma sublateritium Hyeon-Woo Koh, Sudas Rani, and Soo-Je Park* Jongwoon Choi, Hasun Kim, Dongyong Shin, and Yunkyeong Lee* Department of Biology, Jeju National University Forest Research Institute of Gangwon-do A novel strain (designated sjH1T), characterized as aerobic, Gram-stain This study showed to excellent mycelium growing at Glucose in circular negative, oxidase-positive, catalase-negative, motile and rod-shaped, was the carbon which was suitable for the third, mycelium growing of a N. isolated from mine wastewater. Phylogenetic analysis based on 16S rRNA sublateritium provisional results carbon circle regarding a nitrogen circle, gene sequences indicated that strain sjH1T belonged to the genus Rhodanobacter. and cultures of a N. sublateritium looked at malt extract in case of organic Strain sjH1T was closely related to Rhodanobacter thiooxydans LCS2T (98.0% nitrogen circles. However, a kind circular carbon nitrogen used to a test is 16S rRNA gene sequence similarity), Rhodanobacter denitrificans 2APBS1T restrictive, and provisional shall consist of various elements by a foundation. (97.7%), Rhodanobacter soli DCY45T (97.2%), and Rhodanobacter caeni This study looked in a phosphoric acid circle to affect the fourth, a MJ01T (97.0%). The DNA G+C content of strain sjH1T was 69.2 mol%. DNA-DNA mycelium of a N.sublateritium to growth and development. Also, If this relatedness (<60%) indicated that strain sjH1T represents a distinct species study added it was investigated p-aminobenzoic acid in a vitamin circle so that is separate from R. thiooxydans, R. denitrificans, R. soli, and R. caeni. that hypha growing was comparatively good. The major ubiquinone was Q-8, and major fatty acids were summed feature 9 (iso–C17:1 ω9c and/or C16:0 10–methyl), iso–C15:0, iso–C17:0, iso–C16:0, and anteiso–C15:0. Based on data from this polyphasic study, it is proposed that sjH1T (=KCTC 42660T =JCM 30774T) is the type strain of a novel species, Rhodanobacter aciditrophus sp. nov. [Supported by grants from NRF (2015R1C1A1A01053750 & 2015M3D 3A1A01064881)] www.msk.or.kr | 1 2016 International Meeting of the Microbiological Society of Korea A005 A007 A Multiplex-PCR for Rapid Identification of 4 Enterococcus Flavihumibacter sediminis sp. nov., Isolated from Tidal Flat Species Using Species-Specific Primers from Comparative Sediment Genomics Do-Hoon Lee and Chang-Jun Cha* 1 2 2 2,3 2,3 Jongbin Park , Gwi-Deuk Jin , Yong Hyun Kim , Jae In Pak , and Eun Bae Kim * Department of Systems Biotechnology, Chung-Ang University 1Department of Animal Life System, College of Animal Life Sciences, Kangwon National University, 2Department of Animal Life Science, College of Animal Life A yellow-colored, Gram-stain-positive, aerobic, rod-shaped and non-motile Sciences, Kangwon National University, 3Division of Applied Animal Science, bacterial strain, designated AM3T, was isolated from the tidal flat in College of Animal Life Sciences, Kangwon National University Ganghwa-do, South Korea. Strain AM3T optimally grew on R2A at 30oC and pH 7.0 and did not require NaCl for growth. Phylogenetic analysis based Enterococcus species are lactic acid bacteria frequently found in food/feed on 16S rRNA gene sequence similarity showed that strain AM3T belonged and human/animal guts. They are regarded as either commensal bacteria to the genus Flavihumibacter within the family Chitinophagaceae and was or sometimes pathogens. For these reasons, rapid identification is required the most closely related to Flavihumibacter cheonanensis KACC 17467T in both food industries and clinical practices. Here, we investigated a new (98.3%), followed by Flavihumibacter solisilvae KACC 17917T (97.4%). rapid identification method based on species-specific genes from comparative DNA-DNA relatedness levels of strain AM3T were 30.6% to F. cheonanensis genomics for 4 Enterococcus species, 16S rRNA sequences of which are KACC 17467T and 42.6% to F. solisilvae KACC 17917T. The major isoprenoid similar. From genome comparison, we identified species-specific genes quinone was menaquinone (MK-7). The predominant polar lipids were for Enterococcus faecium, Enterococcus faecalis, Enterococcus durans and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Enterococcus hirae, respectively. Four sets of primers were designed for The G+C content of the genomic DNA was 47.7 mol%. On the basic each species. After each primer set was tested and optimized for identification polyphasic taxonomic data, strain AM3T represents a novel species in the of corresponding species, the four primers were re-optimized for Multiplex- genus Flavihumibacter, for which name Flavihumibacter sediminis sp. nov. PCR using already known Enterococcus strains. This method was re-confirmed is proposed. using locally isolated strains of 4 different species from chicken feces, cow feces, horse feces, meats, and fermented soybean. In this study, we designed a species-specific primers distinguishing the 4 Enterococcus spp. in our new Multiplex-PCR method. This will help rapid decision making to get practical benefits in food/feed industries and clinical services. [This study was supported by the Strategic Initiative for Microbiomes in Agriculture and Food, Republic of Korea (914005-04).] Keywords: Enterococcus, Species-specific primer, Multiplex-PCR, Rapid identification, 16S rRNA A006 A008 Investigating the Inflammatory and Phenotypic Responses of Taxonomic Study of the Genus Hymenobacter Multiple Cultured Human Epithelial Cells to Predatory Bacteria Joo Won Kang, Mi Sun Kim, Ji Hee Lee, Seon Choi, Wasimul Bari and Ajay K. Monnappa* Da Hyun Kim, and Chi Nam Seong* Ulsan National Institute of Science and Technology Department of Biology, College of Life Science and Natural Resources, Sunchon National University Bdellovibrio-and-like-organisms (BALOs) are small Gram-negative predatory bacteria that attack and kill other Gram-negative bacteria including many The genus Hymenobacter, a member of the family Cytophagaceae (Stanier, pathogens. Although BALOs are considered to be