1 WCSU BIO 106 NVCC BIO 212 BLOOD VESSELS LAB and ASSIGNMENT Courtesy of: Dr. Susan Maskel Professor of Biology Western Conneccut State University Danbury, CT 06810 Source materials: UCONN site: hp://www.pnb.uconn.edu/PNB_Base/catlas/cardiovascular/uharteries.html Elaine Marieb Lab workbooks and others, as indicated. Pulmonary 2 Circulaon O2 poor blood from heart to lungs; O2 rich blood from lungs to heart SystEmic Circulaon O2 rich blood from heart to body ssues; O2 poor blood from lecture textbook, Fig. 18.1 body ssues to heart Example: SystEmic Circulaon 3 heart ascEnding aorta aorBc arch descEnding aorta renal artery kidney renal vein infErior vEna cava heart ExcEpon: Hepac Portal Circulaon 4 Blood from digesve system picks up nutrients, toxins. Liver monitors nutrients, toxins. Blood from digesve organs goes to liver before returning to heart. digEsvE organs hepac portal vEin livEr hepac vEins infErior vEna cava heart Cat Dissecon 5 Use only side sinks for cats Sink in back of room: people only!!!! 1 cat per 2 students Use same bag & preservave unless leaking 2 tags – in pencil (not pen) – your name, your lab partner’s name; 1 tag on paw; 1 tag on outside of bag about 1/8 cup preservave in bag when stored NO CAT PARTS IN SINK! (if cat parts are found in sink, extra credit will no longer be given on lab quizzes & exams) rinse cat trays when finished clean your staon with desk wash when finished 6 page 700 The cat you receive should look page very mucH 700 like this cat. page 412 7 page 721 Cut open 2 or 2 your cat 3 as 3 Diaphragm page indicated 721 in this 1 4 4 diagram. Incision page 427 In order to find the blood vessels, you need to cut 8 away fat and other connecve ssues. Blunt probes: most useful dissecng tool; use for separang Forceps: use for grasping & pulling ssue Scalpels: cut ssues held by forceps 9 This is what an page 726 excellent dissecon looks like EXCEPT: page 726 (1) do not cut off the body wall. Just move it to the side, page and 432 (2) see next page. 10 Try not to This cut out the picture greater did not omentum come (an apron-like from layer over the your abdominal labbook organs); just push it to the side greater omentum Larynx 11 External jugular vein Trachea Brachiocephalic Vagus nerve vein Superior vena cava (precava) Aorta Heart Diaphragm (cut) Lobe of lung Stomach Lobe of liver Kidney Spleen Small intestine 10th & 11th edions: Large intestine page 726 Urinary bladder Integrated edion: page 432 Arteries 12 R. & L. common carod arteries 13 Larynx Arteries Right common carotid artery Left common carotid artery Right subclavian Left artery Subclavian Artery Brachio- Thymus cephalic artery heart Lungs Diaphragm Picture from cat respiratory system exercise; I changed the labels 10th & 11th ediOons:page 739; Integrated edion: page 443 To find thE artEriEs in thE abdominopelvic cavity: 14 find descending aorta in chest follow dEscEnding aorta down to diaphragm clear off area caudal to diaphragm push abdominal organs to cat’s right first branch off aorta on right = celiac trunk second branch off aorta on right = superior mesenteric artery 15 ileolumbar artery Arteries 16 Celiac trunk Edge of diaphragm Adrenolumbar artery Adrenal gland Superior mesenteric artery Descending abdominal aorta Inferior mesenteric artery Iliolumbar artery Renal artery Right external iliac artery Gonadal artery (testicular or ovarian) part of figure on part of part of figure on figure on page 728 page 728 page 434 Arteries 17 ileolumbar artery Common iliac artery 18 Internal iliac artery NOTE the External iliac artery differences between the Femoral artery cat & the human arteries Human diagram = part of diagram on page: 11th edion: p. 477 10th edion: p. 477 Integrated: p. 289 19 Larynx VEINS Left external jugular vein Right Trachea subclavian Left common carotid artery vein Right Left subclavian vein brachio- Superior vena cava cephalic (precava) vein Thymus Left brachio- cephalic Left ventricle of heart vein Lungs Diaphragm Picture from cat respiratory system exercise; I changed the labels 10th & 11th ediOons:page 739; Integrated ediOon:page 443 20 R. & L. External Jugular R. & L. Internal Jugular VEINS VEINS 21 VEINS 22 23 To find hEpaBc vEins Find where inferior vena cava passes by liver Using scalpel, scrape away liver ssue in that area Hepac veins will appear To find hEpaBc portal vEin Remove peritoneum between first part of small intesne (duodenum) and liver. Hepac portal vein should appear brown Copyright protected 2000, Susan Caley Opsal. This image may be copied and used for educaonal purposes. hp://www2.ivcc.edu/caley/108/lab_images/portal.html 24 C = Hepac Portal Vein (injected with yellow latex) A = inferior mesenteric vein B = superior mesenteric veins D = gastrosplenic vein (you don’t need to know this vein) VEINS 25 L. external iliac vein 26 page VEINS 729 External jugular vein Subclavian vein Brachiocephalic vein Superior vena cava page (precava) Heart 733 Hepatic veins Inferior vena cava (postcava) Adrenolumbar artery and vein Renal artery and vein page Common 439 iliac vein Iliolumbar artery and vein Internal iliac vein Femoral artery and External iliac vein vein 27 Common iliac vein NOTE the Internal iliac vein External iliac vein similaries Femoral vein between the cat & Great Saphenous vein human veins Great saphenous Popliteal vein vein Common iliac vein External iliac vein Small saphenous Popliteal vein vein Internal iliac vein Great saphenous vein Anterior tibial vein Fibular vein Fibular vein Femoral vein Small saphenous Anterior tibial vein vein (superficial) Posterior tibial vein Dorsalis Posterior tibial pedis vein vein Dorsal venous Plantar veins arch upper diagram (human): lower diagram (cat): 11th edion: part of diagram on page 479 Deep plantar arch th Dorsal metatarsal 11 edion: part of diagram on page 731 veins th th 10 edion: part of diagram on page 479 Digital veins 10 edion: part of diagram on page 730 Integrated: part of diagram on page 291 Integrated: part of diagram on page 436 Anterior view Posterior view Figure 19.21b from lecture textbook (minus what you don’t need to know) 28 Arteries of the head and trunk Internal carod artery External carod artery Arteries that supply the upper limb Common carod arteries Subclavian artery Brachiocephalic trunk Axillary artery Subclavian artery Brachial artery Aorc arch Radial artery Ascending aorta Ulnar artery Coronary artery Celiac trunk Abdominal aorta Superior mesenteric artery Arteries that supply the lower Renal artery limb Gonadal artery External iliac artery Inferior mesenteric artery Femoral artery Common iliac artery Internal iliac artery Illustration, anterior view Figure 19.26b from lecture textbook (minus what you don’t need to know) Veins of the head and trunk 29 Veins that drain External jugular vein the upper limb Subclavian vein Internal jugular vein Axillary vein Right and le brachiocephalic veins Brachial vein Superior vena cava Hepac veins Hepac portal vein Ulnar vein Renal vein Radial vein Superior mesenteric vein Inferior mesenteric vein Inferior vena cava Common iliac vein Veins that drain Internal iliac vein the lower limb External iliac vein Femoral vein Great saphenous vein Illustration, anterior view. The vessels of the pulmonary circulaon are not shown. .