Instructions For Use Rotors and Tubes For Beckman Coulter J2, J6, and Avanti J Series Centrifuges PN JR-IM-10AG October 2014 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A. Rotors and Tubes For J2, J6, and Avanti J Series Centrifuges PN JR-IM-10AG (October 2014) © 2014 Beckman Coulter, Inc. All rights reserved No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc. Beckman Coulter, the stylized logo, Avanti, Aerosolve, HarvestLine, Microfuge, and Quick-Seal are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders. Find us on the World Wide Web at: www.beckmancoulter.com Printed in U.S.A. Revision History This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released. Revision AE, December 2013 Added the JA-14.50 rotor: See Table 1.1, Rotors Used in Beckman Coulter J Series Centrifuges and Table 4.1, General Specifications for Beckman Coulter J Series Fixed-Angle Rotors. Revision AF, February 2014 Changed Polyallomer to polypropylene: • CHAPTER 1, Pelleting (Differential Separation) • CHAPTER 2, Characteristics and Chemical Resistances of Tube and Bottle Materials • CHAPTER 2, Labware Types • CHAPTER 3, Gradient Preparation • CHAPTER 2, Gradient Formation and Fractionation • CHAPTER 2, Labware Types • CHAPTER 3, Gradient Preparation • CHAPTER 3, General Filling and Sealing Requirements for Tubes and Bottles • CHAPTER 3, Open-Top Polypropylene Tubes • CHAPTER 3, Capping Tubes • CHAPTER 3, Filling and Sealing Quick-Seal Tubes • CHAPTER 7, Cleaning • CHAPTER 7, Sterilization and Disinfection • CHAPTER 7, Tube and Bottle Sterilization and Disinfection Revision AG, October 2014 Added Avanti JXN-30 Centrifuge, Avanti JXN-26 Centrifuge, and JA-18 Rotor: See CHAPTER 1, Rotors Used in Beckman Coulter J Series Centrifuges. PN JR-IM-10AG iii Revision History iv PN JR-IM-10AG Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative. Alerts for Warning, Caution, Important, and Note WARNING WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury. CAUTION CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed. Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process. NOTE NOTE is used to call attention to notable information that should be followed during installation, use, or servicing of this equipment. This safety notice summarizes information basic to the safe operation of the rotors and accessories described in this manual. The international symbol displayed above is a reminder that all safety instructions should be read and understood before use or maintenance of rotors or accessories. When you see the symbol on other pages, pay special attention to the safety information presented. Also observe any safety information contained in applicable rotor and centrifuge manuals. Observance of safety precautions will help to avoid actions that could cause personal injury, as well as damage or adversely affect the performance of the centrifuge/rotor/tube system. Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in these rotors, however, unless all necessary safety precautions are taken. • Observe all cautionary information printed on the original solution containers prior to their use. • Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize PN JR-IM-10AG v Safety Notice Mechanical Safety the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in the rotor without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. • If disassembly reveals evidence of leakage, you should assume that some fluid escaped the container or rotor. Apply appropriate decontamination procedures to the centrifuge, rotor, and accessories. Mechanical Safety • Use only the rotors, components, and accessories designed for use in the rotor and centrifuge being used (refer to the applicable rotor manual). The safety of rotor components and accessories made by other manufacturers cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in Beckman Coulter rotors may void the rotor warranty and should be prohibited by your laboratory safety officer. • Rotors are designed for use at the speeds indicated; however, speed reductions may be required because of weight considerations of tubes, adapters, and/or the density of the solution being centrifuged. Be sure to observe the instructions in the applicable rotor manual. • NEVER attempt to slow or stop a rotor by hand. • The strength of containers can vary between lots, and will depend on handling and usage. We highly recommend that you pretest them in the rotor (using buffer or gradient of equivalent density to the intended sample solution) to determine optimal operating conditions. Scratches (even microscopic ones) significantly weaken glass and polycarbonate containers. To help prevent premature failures or hazards by detecting stress corrosion, metal fatigue, wear or damage to anodized coatings, and to instruct laboratory personnel in the proper care of rotors, Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your rotors for corrosion or damage. The representative will recommend repair or replacement of at- risk rotors to prevent potential rotor failures. Contact your local Beckman Coulter office to request this service. It is your responsibility to decontaminate the rotors and accessories before requesting service by Beckman Coulter Field Service. vi PN JR-IM-10AG Safety Notice RoHS Notice RoHS Notice These labels and materials declaration table (the Table of Hazardous Substance’s Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements. China RoHS Caution Label — This label indicates that the electronic information product contains certain toxic or hazardous substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and indicates the number of calendar years the product can be in operation. Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture. China RoHS Environmental Label — This label indicates that the electronic information product does not contain any toxic or hazardous substances. The center “e” indicates the product is environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture. PN JR-IM-10AG vii Safety Notice RoHS Notice viii PN JR-IM-10AG Contents Revision History,iii Safety Notice,v Alerts for Warning, Caution, Important, and Note,v Chemical and Biological Safety,v Mechanical Safety,vi RoHS Notice,vii Scope,xvii Scope of this Manual,xvii Rotors,1-1 Introduction,1-1 General Description,1-1 Rotor Designations,1-1 Materials,1-3 Drive Pins,1-4 Rotor Selection,1-4 Pelleting (Differential Separation),1-11 Isopycnic Separations,1-15 Rate Zonal Separations,1-15 Blood Component Separations, 1-16 General Operating Information, 1-16 Rotor Balance, 1-16 Rotor Tie-Down, 1-17 Overspeed Protection,1-18 Allowable Run Speeds, 1-18 Temperature Compensation, 1-19 Avanti J Centrifuges,1-19 Avanti J2 Series Centrifuges (No longer manufactured),1-20 Analog J6 Series Centrifuges (No longer manufactured), 1-20 Microprocessor-Controlled Centrifuges, 1-21 ix Contents Tubes, Bottles, and Accessories,2-1 Introduction,2-1 Labware Selection Criteria ,2-1 Labware