Data Sheet (Cat.No.TN4486) Magnolianin Chemical Properties CAS No.: 147663-91-0 Formula： C54H50O8 Molecular Weight： 827 Appearance： N/A Storage： 0-4℃ for short term (days to weeks), or -20℃ for long term (months). Biological Description Description Magnolianin can inhibit strongly (IC50 = 0.45 uM) 5-HETE formation by die cytosol of guinea pig polymorphonuclear leukocytes. Targets(IC50) NO: None In vitro The methanolic extract from a Japanese herbal medicine, the bark of Magnolia obovata, was found to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophages. METHODS AND RESULTS: By bioassay-guided separation, three neolignans (magnolol, honokiol, obovatol) and three sesquiterpenes (alpha- eudesmol, beta-eudesmol, gamma-eudesmol) were obtained as active constituents. A trineolignan (Magnolianin), a phenylpropanoid glycoside (syringin), lignan glycosides (liriodendrin, (+)-syringaresinol 4'-O- beta-D-glucopyranoside) and a sesquiterpene (caryophyllene oxide) did not show any activity. On the other hand, sesquiterpene-neolignans (eudesmagnolol, clovanemagnolol, caryolanemagnolol, eudeshonokiol A, eudesobovatol A) showed the strong cytotoxic effects. CONCLUSIONS: Active constituents (magnolol, honokiol, obovatol) showed weak inhibition for inducible NO synthase (iNOS) enzyme activity, but potent inhibition of iNOS induction and activation of nuclear factor-kappaB. Solubility Information Solubility < 1 mg/ml refers to the product slightly soluble or insoluble Preparing Stock Solutions 1mg 5mg 10mg 1 mM 1.209 mL 6.046 mL 12.092 mL 5 mM 0.242 mL 1.209 mL 2.418 mL 10 mM 0.121 mL 0.605 mL 1.209 mL 50 mM 0.024 mL 0.121 mL 0.242 mL Please select the appropriate solvent to prepare the stock solution, according to the solubility of the product in different solvents. The storage conditions and period of the stock solution: - 80 ℃ for 6 months; - 20 ℃ for 1 month. Please use it as soon as possible. Reference 1. Effects of constituents from the bark of Magnolia obovata on nitric oxide production in lipopolysaccharide-activated macrophages. Chem Pharm Bull (Tokyo). 2001 Jun;49(6):716-20. Page 1 of 2 www.targetmol.com A DRUG SCREENING EXPERT Inhibitors · Natural Compounds · Compound Libraries This product is for Research Use Only · Not for Human or Veterinary or Therapeutic Use. Tel:781-999-4286 E-mail:[email protected] Address:36 Washington Street,Wellesley Hills,MA 02481 Page 2 of 2 www.targetmol.com.