INVESTIGATIVE UROLOGY Formerly the Journal "Investigative Urology" (1963-1981), Founded by William W. Scott Section Editor Stuart S. Howards Charlottesville, Virginia Editorial Board Joseph A. Smith Salt Lake City, Utah INVESTIGATIVE UROLOGY This Month in Investigative Urology Bladder Physiology. E. J. McGuire 1242 Animal Model of Non-Obstructive Bladder Instability. Κ Κ Sethia, A. F. Brading and J. C. Smith 1243 In Vivo Antiproliferative Effects of Gamma-Interferon and Tumor Necrosis Factor Alpha in a Rat Renal Cell Carcinoma Model System. R. J. A. Van Moorselaar, A, J. M. C. Beniers, B. Th. Hendricks, P. H. Van Der Meide, Η. Schellekens, F. Μ. J. Debruyne and J. Α. Schalken 1247 Development of Liposomes Containing Interferon Alpha for the Intravesical Therapy of Human Superficial Bladder Cancer. D. N. Frangos, J. J. Killion, D. Fan, R. Fishbeck, A. C. von Eschenbach and I. J. Fidler 1252 Augmentation Cystoplasty Utilizing De-Epithelialized Sigmoid Colon: A Preliminary Study. R. C. Motley, Β. T. Montgomery, P. E. Zollman, Κ E. Holley and S. A. Kramer 1257 Suture Material in Bladder Surgery: A Comparison of Polydioxanone, Polyglactin, and Chromic Catgut. D. W. Stewart, P. J. Buffington and J. Wacksman 1261 Norepinephrine Involvement in Penile Detumescence. W. Diederichs, C. G. Stief, T. F. Lue and E. A. Tanagho 1264 Comparison of Urinary Bladder Function in Sexually Mature and Immature Male and Female Rats. A. L. Chun, A. J, Wein, R. Harkaway and R. M. Levin 1267 Epidermal Growth Factor Receptor mRNA Levels in Human Prostatic Tumors and Cell Lines. G. L. Morris and J. G. Dodd 1272 Urinary Solute Transport by Intestinal Segments: A Comparative Study of Ileum and Colon in Rats. M. 0. Koch, E. Gurevitch, D. E. Hill and W. S. McDougal 1275 Acute Physiological Changes in Canine Kidneys Following Exposure to Extracorporeal Shock Waves. S. J. Karisen, B. Smevik, J. Stenstr0m and Κ J. Berg 1280 Investigative Grammar 1284 0022-5347/90/1436-1264S02.00/0 THE JOURNAL OF UROLOGY Vol. 143, June Copyright© 1990 by AMERICAN UROLOGICAL ASSOCIATION, INC. Printed in U.S.A. NOREPINEPHRINE INVOLVEMENT IN PENILE DETUMESCENCE W. DIEDERICHS,* C. G. STIEF, T. F. LUE AND Ε. A. TANAGHO From the Department of Urology, University of California School of Medicine, San Francisco, California ABSTRACT Adrenergic neurotransmission was studied in dogs. Blood samples for catecholamine assay were collected from the flaccid penis, the erect penis after cavernous nerve stimulation and during detumescence induced by sympathetic trunk stimulation. Epinephrine concentration was not significantly different in these three stages. However, norepinephrine concentration increased significantly (p <0.005) during induced detumescence from 505±311 [pg./ml.] to 747±489 [pg./ml.]. Intracavernous application of norepinephrine abolished cavernous nerve induced erection in a dose dependent manner. Sympathetic trunk stimulation reduced or abolished erection induced by acetylcholine or by vasoactive intestinal polypeptide. We conclude that norepinephrine is an important neurotransmitter in the control of penile detumescence. (J. Urol, 143:1264-1266, 1990) Various protocols of neurostimulation1 and intracavernous seconds per pulse; voltage range 1.2 to 3 V; each stimulation (i.e.) injection of various neurotransmitters2 can elicit erection for 60 seconds). and detumescence. For example, acetylcholine (Ach), norepi­ Systemic blood samples (one cc) were collected from the nephrine and vasoactive intestinal polypeptide (VIP) have been cephalic vein ten minutes after application of the premedication extensively used in studies on erection.2,3,4 In dogs, erection (sample 1), from the femoral artery after the surgical set-up can be induced by i.e. application of Ach5, VIP3, and also by (sample 2) and immediately after sympathetic trunk stimula• pelvic6 or cavernous nerve stimulation.7 Penile detumescence tion (STS; sample 3). Penile samples from the corpora caver• has been induced by sympathetic trunk stimulation1,8 and/or nosa were taken before (sample 4) and after sixty seconds of i.e. norepinephrine injection.2 These results suggest that neuro­ cavernous nerve stimulation (CNS; sample 5) as well as thirty stimulation is effective on penile tissue probably via the release to forty seconds after initiation of STS (sample 6). The blood of one of the neurotransmitters mentioned above. was withdrawn into syringes containing EGT A and glutathion, The present study was designed to clarify, in vivo, adrenergic stored in ice, centrifuged (4C, 1000 g for 20 minutes), and the effects on erection. We measured epinephrine (E) and norepi­ plasma separated and stored at 120C. To measure the cat• nephrine (NE) levels in penile blood during erection and de­ echolamine plasma levels a radioenzymatic assay9 was used. tumescence phases induced by neurostimulation. We also com­ The assay can detect one pg. (20 pg./ml. of plasma) of NE or pared effects of neurostimulation to the action of (i.e.) injected E. neurotransmitters. The following neurotransmitters were used for (i.e.) injection after dilution with saline to 0.3 to 0.5 ml.: MATERIALS AND METHOD Acetylcholine: Cooper Vision, Pharmaceutical, Inc. (Dose: 10-50 Mg.) Experiments were performed on sixteen adult male mongrel Norepinephrine: Wintrop-Breon Lab, (Dose: 10-50 μ%.) dogs (19.2 to 43.3 kg.). The animals were premedicated with VIP (porcine synthetic): Sigma (Dose: 10-20 μ%.) acepromazine (0.1 mg./kg.) and ketamine (five mg./kg.) sub- Data are expressed as mean or as mean ± standard deviation cutaneously and anesthetized with pentobarbital (i.V., 130 mg. unless otherwise stated. The concentrations from the systemic as a bolus; additional doses as required). Fluid was maintained blood (sample 1-3 = group 1) as well as from penile blood by infusion of saline at 100 ml./hr. The dogs were placed in the (sample 4-6 = group 2) were compared with a Wilcoxon signed supine position on a water heated pad at 39C, and the lower rank test. Values were considered significantly different when abdomen was exposed by a midline incision. The lumbar sym­ ρ <0.05. pathetic trunks were separated from the aorta and fitted with bipolar cuff electrodes (Avery Laboratories). The right and left RESULTS cavernous nerves were identified close to the prostate, and cuff electrodes were placed around them. The right internal puden­ The intracavernous pressure increased almost ten-fold dal artery was fitted with an ultrasonic flow probe (Transonics within one minute after the start of cavernous nerve stimula­ Systems, Inc., Ithaca, NY). The midshaft of the penis was tion. Recovery to the baseline pressure after the cessation of dissected free from the skin and the subcutaneous layers. stimulation typically required five to six minutes. However, Each corpus cavernosum was punctured with two 19 gauge when the sympathetic trunks were stimulated 10 to 15 seconds scalp vein needles. One needle was used to collect blood samples after the cessation of nerve stimulation, the recovery required from the penis and the other for recording the pressure. Sys­ only on the order of 30 seconds. Rate of recovery was hence temic blood pressure was recorded through a 16 gauge angiocath about ten times faster with as compared to without sympathetic needle placed in the right femoral artery. One scalp vein needle trunk stimulation. from each corpus and the angiocath needle were connected to Surgical stress significantly increased systemic blood concen­ Statham pressure transducers. A Grass polygraph (Model 7) trations of Ε and NE, but neurostimulation did not further was used for recording the pressures. increase catecholamine levels. In penile blood, however, STS A transmitter with an external antenna (Avery Laboratories) significantly increased NE when compared with the concentra­ was used for neurostimulation (parameters: 20 Hz; 200 micro- tion found after CNS (see table 1). Figure 1 A shows the increase (in two distinct steps) in the Accepted for publication January 22, 1990. intracavernous pressure following the i.e. injection of acetyl­ * Requests for reprints: Urologische Klinik, Marienhospital Herne, Universitätsklinik, Ruhr Universität Bochum, Widumerstr. 8, 4690 choline (Ach). Recovery of the baseline pressure generally Herne, West Germany. required on the order of one to two minutes, as shown. 1264 NOREPINEPHRINE INVOLVEMENT IN PENILE DETUMESCENCE 1265 TABLE 1. Epinephrine (Ε) and norepinephrine (NE) values CCp Sample Ε NE [pg./ml.l 160 η Mean SD Mean SD 1 224* 49 298t 158 2 695* 337 803t 416 χ 574 804 487 3 982 Ε 4 426 251 398 264 ο δ 513 270 505§ 311 6 556 304 747§ 489 §,t," Pairs indicated by these symbols consist of two significantly different min. means (p < 0.05). Sample 1: from cephalic vein ten minutes after premedication KSTSH 2: from femoral artery after surgical set up 3: from femoral artery after STS 4: from cavernous body (no stimulation) FIG. 2. VIP induced erection and sympathetic trunks stimulation 5: from cavernous body after CNS (STS) on intracavernous pressure (CCp). 6: from cavernous body after STS CCp 160 π i.e. X Ε ο min. h- CNS -I FIG. 3. Effect of intracavernous injection of norepinephrine (arrow) and cavernous nerve stimulation (CNS) on intracavernous pressure (CCp). Β If the sympathetic trunks were stimulated before, during, CCp and after the injection of 10 ßg. Ach, however, the Ach had no 160 • i.e. effect other than the small changes shown in figure IB. STS partially negated the effect of injection of a much larger dose of Ach (50 μ^). If the sympathetic trunks were stimulated after (instead of I before, during, and after) 10 ßg. Ach injection, the intracaver• Ε ο nous pressure increase was attenuated as compared with no STS (figure 1C). STS had no effect on the i.e. pressure after injection of 50 ßg. Ach. VIP (five dogs). One to three minutes after VIP injection, the min. i.e. pressure increased from about 20 cm. H20 to 90 cm. H20. The concomitant erections lasted between four and twenty minutes.