Supplementary Table 1: Genes affected by anoikis. a, ratio of signal intensity of nonanchored cells anchorage dependent cells (CasKoSrc) over anchored cells; b, induced by Src transformation of Cx43KO cells; c, decreased by Src transformation of Cx43Ko cells; *, induced by normalization of Src transformed cells by neighboring nontransformed cells. Gene Symbol Probe Set Fold Changea Gene Name increased Selenbp1 1450699_at 23.22 selenium binding protein 1 Dscr1l1 1450243_a_at 10.77 Down syndrome critical region gene 1-like 1 Dscr1l1 1421425_a_at 4.29 Down syndrome critical region gene 1-like 1 Ttyh1 1426617_a_at 6.70 tweety homolog 1 (Drosophila) 5730521E12Rik 1419065_at 6.16 RIKEN cDNA 5730521E12 gene c 6330406I15Rik 1452244_at 5.87 RIKEN cDNA 6330406I15 gene AF067063 1425160_at 5.73 clone L2 uniform group of 2-cell-stage gene family mRNA Morc 1419418_a_at 5.55 microrchidia c Gpr56 1421118_a_at 5.43 G protein-coupled receptor 56 Pax6 1452526_a_at 5.06 paired box gene 6 Tgfbi 1415871_at 3.73 transforming growth factor beta induced Adarb1 1434932_at 3.70 adenosine deaminase RNA-specific B1 Ddx3y 1452077_at 3.30 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 Y-linked b Ampd3 1422573_at 3.20 AMP deaminase 3 Gli2 1459211_at 3.07 GLI-Kruppel family member GLI2 Selenbp2 1417580_s_at 2.96 selenium binding protein 2 Adamts1 1450716_at 2.80 a disintegrin-like and metalloprotease with thrombospondin type 1 motif 1 Dusp15 1426189_at 2.70 dual specificity phosphatase-like 15 Dpep3 1429035_at 2.60 dipeptidase 3 Sepp1 1452141_a_at 2.57 selenoprotein P plasma 1 Car9 1427013_at 2.49 carbonic anhydrase 9 H2-T23 1449556_at 2.49 histocompatibility 2 T region locus 23 b Emb 1415857_at 2.46 embigin Acas2l 1437582_at 2.42 acetyl-Coenzyme A synthetase 2 (AMP forming)-like Igh-6 1452574_x_at 2.38 immunoglobulin heavy chain 6 (heavy chain of IgM) Vig1-pending 1421008_at 2.36 viral hemorrhagic septicemia virus (VHSV) induced gene 1 Gng11 1448942_at 2.25 guanine nucleotide binding protein (G protein) gamma 11 C630002M10Rik 1422587_at 2.24 RIKEN cDNA C630002M10 gene Matn2 1419442_at 2.23 matrilin 2 Adamts4 1452595_at 2.22 a disintegrin-like and metalloprotease with thrombospondin type 1 motif 4 c Cdkn1b 1419497_at 2.20 cyclin-dependent kinase inhibitor 1B (P27) Slc40a1 1417061_at 2.19 solute carrier family 40 (iron-regulated transporter) member 1 Col6a3 1424131_at 2.18 procollagen type VI alpha 3 *c Vcam1 1415989_at 2.11 vascular cell adhesion molecule 1 Tafa5-pending 1419490_at 2.09 TAFA5 protein Bcl2l11 1435448_at 2.08 BCL2-like 11 (apoptosis facilitator) Edil3 1433474_at 2.06 EGF-like repeats and discordin I-like domains 3 2310022G12Rik 1424960_at 2.03 RIKEN cDNA 2310022G12 gene decreased A530090O15Rik 1424880_at -4.36 RIKEN cDNA A530090O15 gene Idi1 1423804_a_at -4.15 isopentenyl-diphosphate delta isomerase Idi1 1451122_at -2.97 isopentenyl-diphosphate delta isomerase Met 1422990_at -3.97 met proto-oncogene b Nol5a 1455035_s_at -3.83 nucleolar protein 5A Siva-pending 1418377_a_at -3.80 Cd27 binding protein (Hindu God of destruction) Psmc4 1416290_a_at -3.78 prosome macropain 4 c 1110020L19Rik 1424277_at -3.78 RIKEN cDNA 1110020L19 gene c Chc1l 1431017_at -3.69 chromosome condensation 1-like c 1110018O08Rik 1428882_at -3.44 RIKEN cDNA 1110018O08 gene Loxl3 1436269_s_at -3.22 lysyl oxidase-like 3 Sema3a 1449865_at -3.21 semaphorin 3A b C78541 1423453_at -3.08 expressed sequence C78541 Hip1 1424755_at -3.03 huntingtin interacting protein 1 Abcg5 1419393_at -2.88 ATP-binding cassette G5 Fabp5 1416022_at -2.86 fatty acid binding protein 5 Fabp5 1416021_a_at -2.32 fatty acid binding protein 5 Alg12 1424818_at -2.75 asparagine-linked glycosylation 12 homolog Sc4mol 1423078_a_at -2.74 sterol-C4-methyl oxidase-like b Ppan 1423703_at -2.65 peter pan homolog (Drosophila) Rangap1 1423749_s_at -2.56 RAN GTPase activating protein 1 c Hmgcs1 1433443_a_at -2.54 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 c Hmgcs1 1433446_at -2.10 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 Fdps 1423418_at -2.49 farnesyl diphosphate synthetase Ptgs2 1417262_at -2.45 prostaglandin-endoperoxide synthase 2 c Tpd52l1 1418412_at -2.39 tumor protein D52-like 1 b Ldlr 1421821_at -2.25 low density lipoprotein receptor Psat1 1454607_s_at -2.23 phosphoserine aminotransferase 1 b 2010317E03Rik 1416939_at -2.21 RIKEN cDNA 2010317E03 gene 1110025L05Rik 1434543_a_at -2.15 RIKEN cDNA 1110025L05 gene BE949497 1460713_at -2.12 EST BE949497 0610012A19Rik 1424907_a_at -2.11 RIKEN cDNA 0610012A19 gene b C730024G01Rik 1423577_at -2.11 RIKEN cDNA C730024G01 gene b Srm 1421260_a_at -2.10 spermidine synthase b Akr1b3 1456590_x_at -2.08 aldo-keto reductase family 1, member B3 (aldose reductase) b D11Ertd175e 1416605_at -2.07 DNA segment Chr 11 ERATO Doi 175 expressed BC004738 1451895_a_at -2.03 Mus Musculus mRNA similar to dual specificity phosphatase 9 b Asf1b 1423714_at -2.02 ASF1 anti-silencing function 1 homolog B (S. cerevisiae) 2 Supplementary Table 2: Genes affected by anchorage independence. a, ratio of signal intensity of anchorage independent cells (CasF253Src) over anchorage dependent cells (CasKoSrc); b, induced by Src transformation of Cx43KO cells; c, decreased by Src transformation of Cx43Ko cells. Gene Symbol Probe Set Fold Changea Gene Name increased Igfbp3 1423062_at 36.30 insulin-like growth factor binding protein 3 b Areg 1421134_at 13.15 amphiregulin Dkk2 1420512_at 12.50 dickkopf homolog 2 (Xenopus laevis) 9130004C02Rik 1435042_at 12.30 RIKEN cDNA 9130004C02 gene b Fkbp11 1417267_s_at 9.86 FK506 binding protein 11 c Sox2 1416967_at 8.77 SRY-box containing gene 2 Odz3 1449315_at 7.29 odd Oz/ten-m homolog 3 (Drosophila) Steap 1424938_at 5.21 six transmembrane epithelial antigen of the prostate Il1rl1 1422317_a_at 5.15 interleukin 1 receptor-like 1 Il1rl1 1425145_at 4.12 interleukin 1 receptor-like 1 Msln 1460238_at 4.17 mesothelin Ednra 1451691_at 4.00 endothelin receptor type A c Timp3 1449334_at 3.39 tissue inhibitor of metalloproteinase 3 c Timp3 1449335_at 3.19 tissue inhibitor of metalloproteinase 3 c Timp3 1419089_at 2.95 tissue inhibitor of metalloproteinase 3 Timp3 1419088_at 2.18 tissue inhibitor of metalloproteinase 3 c Fbn1 1460208_at 2.76 fibrillin 1 Ahr 1422631_at 2.73 aryl-hydrocarbon receptor c Osbpl6 1456495_s_at 2.60 oxysterol binding protein-like 6 Akr1c18 1419136_at 2.54 aldo-keto reductase family 1 member C18 b Gfra1 1421973_at 2.48 glial cell line derived neurotrophic factor family receptor alpha 1 b Mmd 1423489_at 2.41 monocyte to macrophage differentiation-associated Clca2 1460259_s_at 2.35 chloride channel calcium activated 2 c A630095P14Rik 1437642_at 2.31 RIKEN cDNA A630095P14 gene Alcam 1426301_at 2.29 activated leukocyte cell adhesion molecule Clca1 1417852_x_at 2.29 chloride channel calcium activated 1 Ptgs2 1417262_at 2.20 prostaglandin-endoperoxide synthase 2 Phex 1421979_at 2.12 phosphate regulating gene homologies to endopeptidases on X chromosome B430320C24Rik 1448024_at 2.07 RIKEN cDNA B430320C24 gene Cth 1426243_at 2.03 cystathionase (cystathionine gamma-lyase) decreased Epha3 1425575_at -176.40 Eph receptor A3 Asb4 1423422_at -10.25 ankyrin repeat and SOCS box-containing protein 4 Asb4 1433919_at -7.29 ankyrin repeat and SOCS box-containing protein 4 b Emb 1415857_at -7.27 embigin 3 2310015I10Rik 1424921_at -4.95 RIKEN cDNA 2310015I10 gene Nid1 1448469_at -4.69 nidogen 1 Nid1 1416808_at -4.03 nidogen 1 Dkk3 1448669_at -4.23 dickkopf homolog 3 (Xenopus laevis) Dkk3 1417312_at -4.13 dickkopf homolog 3 (Xenopus laevis) 2610028F08Rik 1455893_at -4.23 RIKEN cDNA 2610028F08 gene Ctla2a 1448471_a_at -3.94 cytotoxic T lymphocyte-associated protein 2 alpha Ctla2a 1416811_s_at -2.73 cytotoxic T lymphocyte-associated protein 2 alpha Tafa5-pending 1419490_at -3.91 TAFA5 protein b Atox1 1460640_at -3.83 antioxidant protein 1 Tm4sf2 1448737_at -3.52 transmembrane 4 superfamily member 2 Tm4sf2 1417502_at -2.95 transmembrane 4 superfamily member 2 1110028G01Rik 1427201_at -3.14 RIKEN cDNA 1110028G01 gene Txnip 1415996_at -2.82 thioredoxin interacting protein Adamts4 1455965_at -2.47 a disintegrin-like and metalloprotease with thrombospondin type 1 motif 4 c Nedd9 1437132_x_at -2.37 neural precursor cell expressed, developmentally down-regulated gene 9 Dncic1 1416361_a_at -2.34 dynein cytoplasmic intermediate chain 1 c Tgfb2 1423250_a_at -2.31 transforming growth factor beta 2 1810009M01Rik 1418004_a_at -2.04 RIKEN cDNA 1810009M01 gene 4 Supplementary Table 3: Genes affected by migration. a, ratio of signal intensity of anchorage independent migratory cells (CasWtSrc) over anchorage independent nonmigratory cells (CasF253Src); b, induced by Src transformation of Cx43KO cells; c, decreased by Src transformation of Cx43Ko cells; *, induced by normalization of Src transformed cells by neighboring nontransformed cells. Gene Symbol Probe Set Fold Changea Gene Name increased c Sox2 1416967_at 10.27 SRY-box containing gene 2 c Slit2 1424659_at 4.85 Slit2 c Odz3 1449315_at 4.81 odd Oz/ten-m homolog 3 (Drosophila) b Areg 1421134_at 3.68 amphiregulin Akr1c18 1419136_at 3.33 aldo-keto reductase family 1 member C18 b Gfra1 1421973_at 3.18 glial cell line derived neurotrophic factor family receptor alpha 1 Ptgs1 1423414_at 2.93 prostaglandin-endoperoxide synthase 1 AI851155 1448845_at 2.83 expressed sequence AI851155 Dkk2 1420512_at 2.70 dickkopf homolog 2 (Xenopus laevis) BB128963 1452288_at 2.68 expressed sequence BB128963 Ahr 1422631_at 2.68 aryl-hydrocarbon receptor Pkp4 1452209_at 2.51 plakophilin 4 Il1rl1 1422317_a_at 2.33 interleukin 1 receptor-like 1 Il1rl1 1425145_at