THETWO TORT DIT USU NOUTATI20180020671A1 NA MATURIT IN ( 19) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2018 / 0020671 A1 WIGLEY et al. ( 43 ) Pub . Date: Jan . 25 , 2018 ( 54 ) AGRICULTURALLY BENEFICIAL on May 22 , 2015 , provisional application No . 62 / 280 , MICROBES , MICROBIAL COMPOSITIONS, AND CONSORTIA 503 , filed on Jan . 19 , 2016 . (71 ) Applicant : BIOCONSORTIA , INC . , Davis , CA (US ) Publication Classification (72 ) Inventors: Peter WIGLEY , Auckland (NZ ) ; (51 ) Int. CI. Susan TURNER , Davis , CA (US ) ; AOIN 63 / 00 (2006 . 01) Caroline GEORGE , Auckland (NZ ) ; C12N 1 / 20 ( 2006 .01 ) Thomas WILLIAMS, Davis , CA (US ) ; (52 ) U . S . Cl. Kelly ROBERTS , Davis , CA (US ) ; CPC .. .. .. AOIN 63/ 00 (2013 . 01 ) ; C12N 1 /20 Graham HYMUS , Davis , CA (US ) ; ( 2013 .01 ) Kelvin LAU , Auckland ( NZ ) ( 73 ) Assignee : BIOCONSORTIA , INC ., Davis , CA (US ) (57 ) ABSTRACT (21 ) Appl . No. : 15 /549 , 815 The disclosure relates to isolated microorganisms- including (22 ) PCT Filed : Feb . 9 , 2016 novel strains of the microorganisms -microbial consortia , and agricultural compositions comprising the same. Further ( 86 ) PCT No. : PCT/ US2016 /017204 more , the disclosure teaches methods of utilizing the described microorganisms, microbial consortia , and agricul $ 371 ( c ) ( 1 ) , tural compositions comprising the same, in methods for ( 2 ) Date : Aug . 9 , 2017 imparting beneficial properties to target plant species. In Related U . S . Application Data particular aspects , the disclosure provides methods of (60 ) Provisional application No. 62/ 113 , 792 , filed on Feb . increasing desirable plant traits in agronomically important 9 , 2015 , provisional application No . 62 / 165 ,620 , filed crop species. Patent Application Publication Jan . 25 , 2018 Sheet 1 of 11 US 2018 / 0020671 A1 FIG . 1 Microde source - * * Bronnentassortie de obecontemning orie e alegerile ese te ???????? Plant enrichment Grow target plants in sample soljsubstrate Directed selection Prepare extract containing plant- associated microbes eg was roots and crush ( stuizosphere ) or surface sterilise mant material & qush endapoxes Use extract to inoculate new seed or plants Grow plants with or without stressor , screen and select plants exhibiting desired phenotypic traites axcreased bromass under fox Vor availabe por X : increased resistance to pest and disease challenge , improved metabolite expression es concentration of foliar sugars ffofote CFOBES collate microbes, selectively and ; or non se echives Green isolates for boddett Test isolated microbes afone Quor in combination for ability to induce or comfer desired plant phenotypic trait Patent Application Publication Jan . 25 , 2018 Sheet 2 of 11 US 2018 / 0020671 A1 FIG . 2 Generate diverse plant- microbe associations in MierobeWebapture capture there target te diverse crop plante microbe associations in Multiple rounds of iterative selection of Directed selection plants / microbiomes best expressing the desired trait MierobeMicrobe holationisolation Onon a a range range of ofmedia media SaveSelece the iconoto ves Singlesingle organismsoreomsons andanelor / or consortiaconsenta Traitalt validationvalitation Identifyudentify leading microbesmicrobes (consortia consortiai* a ) Patent Application Publication Jan . 25 , 2018 Sheet 3 of 11 US 2018 / 0020671 A1 FIG . 3 " plant * "HE extract notitit, noculate rossos S best plant Isolate microbes Microbe source Microbe capture Plant- microbe selection Mie obe isolation rellemultiple substrates) multiple rounds) outono Isolates 17 :13 Patent Application Publication Jan . 25 , 2018 Sheet 4 of 11 US 2018 / 0020671 A1 FIG . 8 High 8: throughput Sequencing saxed w XXXX Microbes XXXXX merableme Microbial Consortia BO BR BR JE NA 430 430 24 ittihistamine ACB ACE aco 600 COEN mmmmmmmmmmmmmmmmmmmmmmning 1808 180€ 223323232323232323231 ABCDE imming Bestest plantsplants Isolated microbes Best treatments Purity and identify Prepare treatments Re - screen plants microbes S (reconstruct Prepare treatmentscommunities. ) determine best treatments Patent Application Publication Jan. 25 , 2018 Sheet 5 of 11 US 2018/ 0020671A1 . … instation… SSSSSS … …… … ? : Casis: . … … ?? : ABS C *.'" 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