COLD SPRING HARBOR LABORATORY Zi\MMUL REPON1' 'UOSE a> -JCccLC CC030CC 0cnflCC ANNUAL REPORT 1995 Cold Spring Harbor Laboratory Box 100 1 Bungtown Road Cold Spring Harbor, New York 11724 Book design Emily Harste Managing Editor Susan Cooper Editor Dorothy Brown Photography Margot Bennett, Edward Campodonico, Bill Dickerson, Marlene Emmons, Scott McBride, Herb Parsons Typography Elaine Gaveglia, Susan Schaefer Front cover: A view of the Laboratory from the shore of Cold Spring Harbor. (Photographed by Mar lena Emmons.) Back cover: Poster session at a Cold Spring Harbor scientific meeting in Vannevar Bush Auditorium. (Photographed by Marlene Emmons.) Section title pages: Martens Emmons Contents Officers of the Corporation/Board of Trustees v Governance and Major Affiliations and Committeesvi Lita Hazen (1909-1995), vii PRESIDENT'S REPORT 1 DIRECTOR'S REPORT 9 HIGHLIGHTS 13 ADMINISTRATION REPORT 35 RESEARCH 41 Tumor Viruses 44 Reflections Yakov Gluzman 1948-1996 42 Molecular Genetics of Eukaryotic Cells76 Genetics 100 Structure and Computation142 Neuroscience 160 CSH Laboratory Fellows 187 Author Index197 COLD SPRING HARBOR MEETINGS 199 Symposium on Quantitative Biology 202 Meetings 204 Postgraduate Courses 221 Seminars257 Undergraduate Research259 Nature Study 261 BANBURY CENTER 263 Director's Report264 Meetings 271 DNA LEARNING CENTER 301 COLD SPRING HARBOR LABORATORY PRESS 319 FINANCE 327 Financial Statements 328 Financial Support 331 Grants331 Capital and Program Contributions338 Annual Contributions339 LABORATORY STAFF 348 Standing (from left) G. Blobel, W.R. Miller, B. Stillman, J.J. Phelan, J.A. Reese, B.D. Clarkson, J.A. Movshon, W.E. Murray, T.J. Knight, O.T. Smith, D.G. Wiley Sitting (from left): J.P. Cleary, E.S. Marks, H. Wendt, M.D. Lindsay, J.A. Steitz, D.L. Luke Ill, D.A. Warner Ill, J.D. Watson, W.D. Pidot, M.V.P. Russell, L.N. Grace, G.R. Fink Not shown: S.M.C. Tilghman, E.A.F. Wimmer, C.F. Dolan, M.F. Gerry, D.H. Koch, W.S. Robertson, T.A. Saunders Ill, R.L. Cummings, H.B. Glass, W.H. Page Officers of the Corporation David L. Luke Ill, Chairman James D. Watson, Ph.D., President Mary D. Lindsay, Vice Chairman Bruce Stillman, Ph.D., Director John R. Reese, Treasurer G. Morgan Browne, Administrative Director Wendy V.P. Russell, Secretary Board of Trustees Scientific Trustees Gunter Blobel, M.D., Ph.D. Martha Farish Gerry Wendy V.P. Russell Rockefeller University Mill Neck, New York Oyster Bay, New York Gerald R. Fink, Ph.D. Lola N. Grace Thomas A. Saunders III Whitehead Institute for Biological Glen Head, New York Locust Valley, New York Research David H. Koch Owen T. Smith, Esq. J. Anthony Movshon, Ph.D. New York, New York Laurel Hollow, New York Howard Hughes Medical Institute New York University Center for Mary D. Lindsay Douglas A. Warner III Neural Science New York, New York Oyster Bay, New York Joan A. Steitz, Ph.D. James D. Watson, Ph.D. David L. Luke III Yale University School of Medicine Cold Spring Harbor Laboratory New York, New York Bruce Stillman, Ph.D. Henry Wendt Edwin S. Marks Cold Spring Harbor Laboratory Healdsburg, California Kings Point, New York Shirley M.C. Tilghman, Ph.D. Howard Hughes Medical Institute William R. Miller Honorary Trustees Princeton University New York, New York Bayard Clarkson, M.D. Don C. Wiley, Ph.D. William E. Murray Memorial Sloan-Kettering Cancer Harvard University New York, New York Center, New York, New York Eckhard A.F. Wimmer, Ph.D. John J. Phelan, Jr. Robert Cummings State University of New York, Mill Neck, New York Glen Head, New York Stony Brook Whitney D. Pidot H. Bentley Glass, Ph.D. Individual Trustees Locust Valley, New York Boulder, Colorado John P. Cleary, Esq. John R. Reese Townsend J. Knight Oyster Bay, New York Cold Spring Harbor, New York New York, New York Charles F. Dolan William S. Robertson Walter H. Page Oyster Bay, New York Huntington, New York Cold Spring Harbor, New York Governance and Major Affiliations The Laboratory is governed by a 30-member Board of Trustees which meets three or four times a year. Authority to act for the Board of Trustees between meetings is vested in the Executive Committee of the Board of Trustees. The Executive Committee is composed of the Officers of the Board plus any other members who may be elected to the Executive Committee by the Board of Trustees. Additional standing and ad hoc committees are appointed by the Board of Trustees to provide guidance and advice in specific areas of the Laboratory's operations. Representation on the Board of Trustees itself is divided between community representa- tives and scientists from major educational and research institutions. The Laboratory is chartered as an educational and research institution by the Board of Regents of the Education Department of the State of New York. It is designated as a "public charity" by the Internal Revenue Service. Committees Audit Development Whitney D. Pidot Wendy V.P. Russell, Chairman Thomas A. Saunders Ill William R. Miller, Chairman G. Morgan Browne Lola N. Grace Susan Cooper Ex Officio John J. Phelan Martha F. Gerry G. Morgan Browne Whitney D. Pidot Townsend J. Knight David L. Luke Ill Thomas A. Saunders Ill Lauri J. Landeau, V.M.D. Bruce Stillman Mary D. Lindsay Banbury Program David L. Luke Ill Nominating James D. Watson, Ph.D, Eckard Wimmer, Ph.D., Chairman Mary D. Lindsay, Chairman Gerald R. Fink, Ph.D. David L. Luke Ill Martha F. Gerry Executive Committee William R. Miller J. Anthony Movshon, Ph.D. David L. Luke Ill, Chairman John R. Reese Jan A. Witkowski, Ph.D. G. Morgan Browne Douglas A. Warner III John P. Cleary, Esq. Building Mary D. Lindsay Robertson House Helen A. Dolan, Chairman William R. Miller William S. Robertson, Chairman Arthur Brings John J. Phelan G. Morgan Browne Martha F. Gerry John R. Reese Anne Meier Mary D. Lindsay Wendy V.P. Russell Jack Richards Jack Richards Bruce Stillman, Ph.D. Jan Witkowski, Ph.D. Wendy V.P. Russell James D. Watson, Ph.D. Owen T. Smith Robertson Research Fund Elizabeth Watson Finance and Investment John R. Reese, Chairman John R. Reese, Chairman G. Morgan Browne Commercial Relations G. Morgan Browne David L. Luke Ill William R. Miller, Chairman William D. Keen Bruce Stillman, Ph.D. Gerald R. Fink, Ph.D. David H. Koch James D. Watson, Ph.D. Nouria Hernandez, Ph.D. David L. Luke Ill David H. Koch Edwin S. Marks Family Representatives David L. Luke Ill William R. Miller Katherine Ernst John Maroney William E. Murray Walter Meier, M.D. John J. Phelan William S. Robertson William S. Robertson Bruce Stillman, Ph.D. Thomas A. Saunders Ill Carl Schafer Douglas A. Warner Ill Henry Wendt Land Use and Preservation Tenure and Appointments John R. Reese, Chairman Gerald R. Fink, Ph.D., Chairman Compensation Owen T. Smith, Vice Chairman Gunter Blobel, M.D., Ph.D. David L. Luke Ill, Chairman Douglas A. Warner, Vice Chairman Joan A. Steitz, Ph.D. Mary D. Lindsay David H. Koch Bruce Stillman, Ph.D. William R. Miller Mary D. Lindsay Shirley Tilghman, Ph.D. John R. Reese William E. Murray Don C. Wiley, Ph.D. vi Lita Annenberg Hazen (1909-1995) Lita liked me for my brains and also because as a successful scientist I was optimistic and never worried her about what I should do next. But she did not like to see me becom- ing almost fat after I took on leading the Human Genome Project and had one job more than I could handle. I then would sense her displeasure when I sat down opposite her at her Pierre Hotel apartment and she would look at my belt not adequate for its job. So when I again became trim, like a long thin DNA molecule, we would not have that awkward start to our conversations where she expressed sharp, clear thoughts and never exuded unreasonable goodliness when the facts were awful. We had first met when I was badly in need of big money to move the Cold Spring Har- bor Laboratory beyond the gene and onto the ultimate challenge of biology, the human mind. I had long known of her philanthropy to biology and medicine, but despite urgings from a colleague who said that our souls were made for each other, I was initially scared to approach her. At a gala several years earlier,I had already gazed upon the firm regality that had emerged out of her Annenberg intellect and money. Yet she could be the friend and patroness who would let me have aspirations beyond common sense. So I got up my courage and began to let her know me, often in the California beauty of her Homby Hills home overlooking the Los Angeles Country Club. At first I assumed that her penchant toward biology brains arose out of the tragedy of her eldest daughter Gwynne's premature death from leukemia and her subsequent endowing of a cancer re- search laboratory at the nearby UCLA Medical Center. In fact, she had to live with the knowledge that it had been her early interest in medical research that had led her to a specialist who used X-rays to treat her daughter's intractable eczema in the days before the cancer-causing attributes of ionizing radiation were firmly established. vii Turning away from rationality was not, however, Lita's thing and much happiness in her later life came from bringing together the scientific elite in groups small enough to let her know whom she was helping. For several years, she also provided a major prize in clinical research, but felt almost betrayed when the recipients of her generosity seemed to vanish from her life almost as quickly as they entered her world at the prize ceremony dinners.