NATIONALINSTITUTE OF GENETICS JAPAN ANNUAL REPORT No. 23 1972 PublitJhMl 611 THE NATIONAL INSTITUTE OF GBNE'l1C8 IIiaimG. 8&uoktl-km, J.,.,. 1973 Annual Report of the National Institute of Genetics No. 23, 1972 I Published by The National Institute of Genetics, Japan 1973 CONTENTS General statement 1 Staff 2 Council.......... .. 5 Association for propagation of the knowledge of genetics .. 6 Projects of research for 1972 7 Researches carried out in 1972 .................................. 11 I. Molecular Genetics The 5'-terminal structure of cytoplasmic polyhedrosis virus (CPV) genome RNA. MIURA, K., SUGIURA, M. and WA- TANABE, K. 11 Terminal structure of single-stranded RNA transcribed by the cytoplasmic polyhedrosis (CP) virus. SHIMOTOHNO, K., FURUICHI, Y. and MIURA, K. 12 The 3'-terminal structure of Avian reovirus RNA. FURUICHI, Y. and MIURA, K. 13 Nucleotide ratio of the genome segments in a cytoplasmic polyh­ edrosis virus from silkworm. FURUICHI, Y., RAI, U. and .. MIURA, K. 13 II. Microbial Genetics Absence of messenger ribonucleic acid specific for flagellin in fla- mutants. SUZUKI, H. and IINO, T. 15 Ill. Biochemical Genetics Improved method for separation and identification of rat serum transferrins: Thin layer acrylamide-gel electrophoresis with acrinol pretreatment. MORIWAKI, K. .. .................. 17 The second scientific expedition to Southeast, Southwest and Central Asia for the study of rodents. IV. Electrophoretic survey of polymorphic transferrin types on the black rat, Rattus rattus. MORIWAKI, K., TSUCHIYA, K., KATO, H., YOSIDA, T. H. and SADAIE, T. 18 ii ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 23 Serum transferrin polymorphism in the red-backed vole, Clethrio­ nomys rufocanus bedfordiae. MORlWAKl, K. and KuwAHATA, T. 22 Zymograms of serum esterases in the red-backed vole (Clethrio­ nomys rufocanus bedfordiae) separated by starch-gel elec- trophoresis. KUWAHATA, T. and MORlWAKl, K. 23 A consideration on the intensity ratio of allelic dimer isozymes in heterozygotes. ENDO, T. 24 The genic system controlling acid phosphatase isozymes in Oryza sativa and O. perennis. PAl, C. and ENDO, T. 25 Amino acid sequence ofhuman J( chain. SHINODA, T. .......... 26 Functional heterogeneity of the variable and constant regions of human Bence-Jones proteins. SHlNODA, T. and TSUZUKIDA, Y. 27 IV. Developmental Genetics Time-lapse cinemicrographic analyses of embryonic cell behaviors of Drosophila melanogaster in culture. KURODA, Y. 29 Inhibition by cyclic AMP and dibutyryl cyclic AMP of aggrega- tion ofembryonic quail liver cells in rotation culture. KURO- DA, Y. 30 Effects of dextran sulfates on aggregation of embryonic quail liver cells in rotation culture. KURODA, Y. 31 Differential inhibition by fucoses and concanavalin A of aggrega- ; tion of normal liver and hepatoma cells in rotation culture. KURODA, Y. 33 Colony-forming activity of embryonic human diploid cells in I culture. KURODA, Y. 34 Effects of 8-azaguanine, EMS and MNNG on colony-forming activity of embryonic human diploid cells in culture. Ku- RODA, Y. 36 Further studies of effects of high molecular serum fractions on growth of mammalian cells in culture. MlNATO, K. 37 Genetic analysis of developmental mechanism in hydra. SUGI- YAMA, T. 38 CONTENTS iii V. Cytogenetics The second scientific expedition for the study of rodents to Southeast, Southwest and Central Asia, I. Members, aims and schedule of the expedition. YOSIDA, T. H. 39 The second scientific expedition for the study of rodents to South­ east, Southwest and Central Asia, II. Animal species col­ lected from Southeast, Southwest and Central Asia, and their chromosome numbers. YOSIDA, T. H., MORIWAKI, K., KATO, H., TSUCHIYA, K. and SAGAI, T. 42 The second scientific expedition for the study of rodents to Southeast, Southwest and Central Asia, III. Karyotype analysis of the black rat, Rattus rattus, collected in Southwest and Central Asia. YOSIDA, T. R., KATO, H., TSUCHIYA, K., MORIWAKI, K. and SAGAI, T. 43 Giemsa banding patterns of chromosomes in seven Rattus species. YOSIDA, T. H. and SAGAI, T. 45 Stable telocentric chromosomes produced by centric fussion in Chinese hamster cells in vitro. KATO, H., SAGAI, T. and YOSI- DA, T. H. 46 Non-random localization of the centromere In mammalian chromosomes. IMAI, H. T. 46 Proposal of a new criterion for the classification of mammalian chromosomes. IMAI, H. T. 50 Comparison between the length method and the weight method for formulating the centromeric position of mammalian chromosomes. IMAI, H. T. 53 • Studies on chromosomes of the silkworm, Bombyx mori. Mu- RAKAMI, A. and IMAI, H. T. 54 The sixth cell population change to occur during serial passages of the MSPC-I mouse myeloma. MORIWAKI, K. and SADAIE, T. .. 56 Ecological genetic studies on the differentiation of Chrysanthemum species. NAGAMI, S. 58 VI. Mutation and Mutagenesis in Animals A new sensitive test system for the detection of weak chemical mutagens using silkworm oocytes. TAZIMA, Y. and ONI- MARU, K. 60 iv ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 23 Test for mutagenicity of mercuric and cadmium compounds using oocyte system of the silkworm. TAZIMA, Y. and FUKASE, Y. 62 Consequences of the selection for mutation frequencies pre­ sumably due to replication instability in the descendants of mitomycin-C treated silkworm. ONIMARU, K. and TAZIMA, Y . 63 Further studies on the instable pM allele of the silkworm. 1. Evidence for the occurrence of recombination between translocated and free second chromosomes in the female. TAZIMA, Y. and OHNUMA, A . 64 X-ray-induced crossing-over in oogenesis of the silkworm. MURAKAMI, A . 66 A comparison of relative mortalities by 2 MeV neutrons and X-rays of young and aged dormant silkworm embryos. MURAKAMI, A. and IWASAKI, T. 67 Mutagenesis of acridine orange in cleavage nuclei ofthe silkworm. MURAKAMI, A . 69 Mutagenesis of quinacrine compounds in the silkworm. MURA- KAMI, A . 70 VII. Radiation Genetics and Chemical Mutagenesis in Microorganisms and Plants Participation of DNA polymerase I in the repair of r-ray damaged DNA in permeable Bacillus subtilis cells. NOGUTI, T. and KADA, T. 72 Purification ofan enzyme specifically involved in repair ofgamma- ray irradiated DNA. NOGUTI, T. and KADA, T. 73 Mapping of recombination-deficient genes in Bacillus subtilis. SADAIE, Y. and KADA, T.. ............................... 74 Efficient procedures for detection offrameshift mutagens. KADA, T. and SADAIE, Y. 75 Radiation effect upon sex expression in cucumber. FUJII, T. .... 76 Further studies on the fractionation dose effects with r-rays in maize pollen. FUJII, T. .. .............................. 78 Modification of radiation damage in rice. BAGCHI, S. and FUJII, T. 79 CONTENTS v Copper dependency of an induced dwarf rice mutant. SHAMA RAO, H. K., FUJII, T. and KADA, T. 80 Radiation and giberellin effects in the induced dwarf rice mutants. SHAMA RAO, H. K. and KADA, T. 81 Biological effects of neutrons after post-irradiation storage or with gamma-ray and neutron combination treatments in rice. SHAMA RAO, H. K. 82 Genetic fine structure analysis of wx locus in maize. AMANO, E. 84 Mixed pollination for obtaining inbred mutant line in maize. AMANO, E. 84 Some experiments with cultured Haplopappus cell lines. AMANO, E. 85 VIII. Population Genetics (Theoretical) Population genetics, molecular biometry, and evolution. KIMURA, M. and OHTA, T. ..•.................................. 87 The effective number of a population with overlapping genera­ tions: a correction and further discussion. CROW, J. F. and KIMURA, M. 87 Fixation probability of a mutant influenced by random fluctua- tion of selection intensity. OHTA, T. 88 Fixation time of overdominant alleles influenced by random fluctuation of selection intensity. OHTA, T. and KIMURA, M. 89 Simulation experiments of multilocus system. YAMAZAKI, T. " and KIMURA, M. 89 IX. Population Genetics (Experimental) Spectra of heterozygosities and of evolutionary rates among different loci. MARUYAMA, T. and YAMAZAKI, T. 91 Evidence that enzyme polymorphisms are selectively neutral. YAMAZAKI, T. and MARUYAMA, T. 93 Further evidence for the neutral hypothesis of protein polymor- phism. YAMAZAKI, T. and MARUYAMA, T. 94 Allelism between lethal genes in Japanese and Korean natural populations of Drosophila melanogaster. OSHIMA, C., vi ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 23 WATANABE, T. K. and CHOO, J. K. 96 Allelism between sterility genes in Japanese and Korean natural populations of D. melanogaster. OSHIMA, C., WATANABE, T. K. and CHOO, J. K. 98 Effects of density of larvae and temperature on a factor of fitness in Drosophila melanogaster. OHNISHI, S. and OSHIMA, C. .. 100 X. Evolutionary Genetics Population size and rate of evolution. OHTA, T. 102 Evolutionary rate ofcistrons and DNA divergence. OHTA, T. .. 102 On the stochastic model for estimation of mutational distance between homologous proteins. KIMURA, M. and OHTA, T. .. 103 Xl. Human Genetics Effect of changing parental age patterns on chromosomal aber- rations and mutations. MATSUNAGA, E. 104 Genetic effect of selective abortion upon the t(2lqDq) transloca- tion. MATSUNAGA, E. 105 Evidence for a second structural locus determining tetrazolium oxidase in man. SHINODA, T., MATSUNAGA, E. and KOSHI- NAGA, J. 106 Isozyme variations in man. SHINODA, T., MATSUNAGA, E. and KOSHINAGA, J. 107 Structural studies on human immunoglobulins. SHINODA, T. .. 108 Prenatal detection of genetic disorders: A program in Shizuoka prefecture. (A preliminary report).