Product datasheet [email protected] ARG51784 Package: 100 μl, 50 μl anti-MEF2A phospho (Ser408) antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes MEF2A phospho (Ser408) Tested Reactivity Hu, Ms, Rat Tested Application IHC-P Host Rabbit Clonality Polyclonal Isotype IgG Target Name MEF2A Antigen Species Human Immunogen Peptide sequence around phosphorylation site of serine 408 (P-I-S(p)-P-P) derived from Human MEF2A. Conjugation Un-conjugated Alternate Names RSRFC4; RSRFC9; ADCAD1; mef2; Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1 Application Instructions Application table Application Dilution IHC-P 1:50 - 1:100 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 55 kDa Properties Form Liquid Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non- phosphopeptide. Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Concentration 1 mg/ml Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol MEF2A Gene Full Name myocyte enhancer factor 2A Background The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain Function Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress- induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. [UniProt] Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody Images ARG51784 anti-MEF2A phospho (Ser408) antibody IHC-P image Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51784 anti-MEF2A phospho (Ser408) antibody (left) or the same antibody preincubated with blocking peptide (right). www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).