Beverages Applications Notebook Additives in Beverages Table of Contents Index of Analytes ........................................................................................................................................................................ 3 Introduction to Beverages .......................................................................................................................................................... 4 UltiMate 3000 UHPLC+ Systems .............................................................................................................................................. 5 IC and RFIC Systems ................................................................................................................................................................. 6 MS Instruments .......................................................................................................................................................................... 7 Chromeleon 7 Chromatography Data System Software ........................................................................................................... 8 Process Analytical Systems and Software ................................................................................................................................. 9 Automated Sample Preparation ......................................................................................................................................... 10–11 Analysis of Additives in Beverages ....................................................................................................................................... 12 Determination of Sulfite in Food and Beverages by Ion Exclusion Chromatography with Pulsed Amperometric Detection ....................................................................................................................................... 13 Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography ............................................... 18 Determination of Steviol Glycosides by HPLC with UV and ELS Detections ...................................................................... 23 Fast HPLC Analysis of Dyes in Foods and Beverages ........................................................................................................... 31 Steviol Glycoside Determination by HPLC with Charged Aerosol and UV Detections Using the Acclaim Trinity P1 Column.............................................................................................................................. 38 Simultaneous Analysis of Water-Soluble Vitamins in Vitamin-Enriched Beverages and Multivitamin Dietary Supplements by UHPLC-MS/MS .......................................................................................... 46 Mogroside V Determination by HPLC with Charged Aerosol and UV Detections ............................................................... 53 Column Selection Guide ........................................................................................................................................................ 58 Column Selection Guide and Specifications............................................................................................................................ 59 Index of Analytes Benzoate ......................................................................................... 18 Dyes ................................................................................................ 31 Mogroside V ................................................................................... 53 Steviol Glycosides ....................................................................23, 38 Sulfite .............................................................................................. 13 Vitamins .......................................................................................... 46 3 Index of Analytes Introduction to Beverages The global beverage industry is growing each year carbohydrates and sugar alcohols, to vitamins, additives, with the introduction of new products, such as vitamin- and sugar substitutes, we are unique in our commitment to fortified water, energy drinks, anti-aging water, and provide fast, accurate testing and labeling information for herbal nutritional supplements. With this growth, come all applications in this industry. many more analytical challenges. These challenges are Thermo Scientific and Dionex Integrated Systems compounded by the continuing and new needs to analyze Dionex Products are now a part of the Thermo classic favorites such as sodas, fruit juices, milk drinks, Scientific brand, creating exciting new possibilities for alcoholic beverages, and bottled water. One such example scientific analysis. Now, leading capabilities in liquid would be the melamine contamination in milk and infant chromatography (LC), ion chromatography (IC), and milk formula. sample preparation are together in one portfolio with For all beverages, the compositional quality and those in mass spectrometry (MS). Combining Dionex’s safety must be monitored to help track contamination, leadership in chromatography with Thermo Scientific’s adulteration, product consistency, and to ensure regulatory leadership position in mass spec, a new range of powerful compliance from raw ingredients (water, additives, and and simplified workflow solutions now becomes possible. fruits) to the final product. For more information on how the new lineup of Thermo Fisher Scientific is a recognized leader in Thermo Scientific products can expand your capabilities providing analytical solutions for sample preparation, and provide the tools for new possibilities, choose one of liquid chromatography for compositional testing, and our integrated solutions: chromatography data management for compliance and quality testing of beverages. From inorganic ions, • Ion Chromatography and Mass Spectrometry organic acids, biogenic amines, glycols and alcohols, • Liquid Chromatography and Mass Spectrometry • Sample Preparation and Mass Spectrometry 4 Introduction UltiMate 3000 UHPLC+ Systems Best-in-class HPLC systems for all your • Thermo Scientific Dionex Viper and nanoViper–the chromatography needs first truly universal, fingertight fitting system even at UHPLC pressures Thermo Scientific Dionex UltiMate 3000 UHPLC+ Systems provide excellent chromatographic performance Thermo Fisher Scientific is the only HPLC company while maintaining easy, reliable operation. The basic uniquely focused on making UHPLC technology available and standard analytical systems offer ultra HPLC to all users, all laboratories, and for all analytes. (UHPLC) compatibility across all modules, ensuring Rapid Separation LC Systems: The extended flow- maximum performance for all users and all laboratories. pressure footprint of the RSLC system provides the Covering flow rates from 20 nL/min to 10 mL/min with performance for ultrafast high-resolution and conventional an industry-leading range of pumping, sampling, and LC applications. detection modules, UltiMate™ 3000 UHPLC+ Systems provide solutions from nano to semipreparative, from RSLCnano Systems: The Rapid Separation nano conventional LC to UHPLC. LC System (RSLCnano) provides the power for high- resolution and fast chromatography in nano, capillary, and • Superior chromatographic performance micro LC. • UHPLC design philosophy throughout nano, standard analytical, and rapid separation liquid Standard LC Systems: Choose from a wide variety of chromotography (RSLC) standard LC systems for demanding LC applications at • 620 bar (9,000 psi) and 100 Hz data rate set a new nano, capillary, micro, analytical, and semipreparative benchmark for basic and standard analytical systems flow rates. • RSLC systems go up to 1000 bar and data rates up to Basic LC Systems: UltiMate 3000 Basic LC Systems 200 Hz are UHPLC compatible and provide reliable, high- • ×2 Dual System for increased productivity solutions performance solutions to fit your bench space and in routine analysis your budget. • Fully UHPLC compatible advanced chromatographic techniques 5 Liquid Chromatography Systems IC and RFIC Systems A complete range of ion chromatography solutions for Dionex ICS-5000: Developed with flexibility, modularity, all customer performance and price requirements and ease-of-use in mind, the Dionex ICS-5000 combines the highest sensitivity with convenience For ion analysis, nothing compares to a Thermo Fisher Scientific ion chromatography system. Whether Dionex ICS-2100: An integrated Reagent-Free IC you have just a few samples or a heavy workload, whether (RFIC™) system for electrolytically generated isocratic your analytical task is simple or challenging, we have a and gradient separations with conductivity detection, now solution to match your needs and budget. And with your with electrolytic sample preparation. IC purchase, you get more than just an instrument—you Dionex ICS-1600: The Dionex ICS-1600 combines get a complete solution based on modern technology and high sensitivity with convenience. Now ready for eluent world-class support. regeneration, with available dual-valve configuration for • Thermo Scientific Dionex ICS-5000: The world’s automated sample preparation. first capillary IC system Dionex ICS-1100: With dual-piston pumping and • Dionex ICS-2100: Award-winning integrated electrolytic suppression. Now ready for eluent Reagent-Free™ IC system regeneration, with available dual-valve configuration for • Dionex ICS-1600: Standard integrated