49th Jírovec's Protozoological Days Conference Proceedings Faculty of Science Charles University Prague, Czech Republic 2019 th 49 Jírovec's Protozoological Days Conference Proceedings Faculty of Science, Charles University, 2019 FOREWORD Dear protistologists, colleagues, and friends! Welcome to the, nearly jubilee, 49th Jírovec‟s Protozoological Days, an international protistological conference, traditionally organized by the Protozoological section of the Czech Society for Parasitology. This year„s Protodays are organized by a group from The Faculty of Science, Charles University, where 90 years ago Otto Jírovec received his doctorate and set out the foundations of Czech protistology. Since 1969, the conference has been an opportunity for graduate and postgraduate students, as well as postdocs and young scientists to present their research and results to the scientific community and the international community of protistologists. The topics presented at the conference include areas such as protistology, cell and molecular biology, genomics, phylogeny and systematics, as well as parasitology, biochemistry, and evolutionary biology and include discoveries of significant new organisms, metabolic pathways of organisms important to our understanding of the origin and evolution of life, research on the causes of serious parasitic diseases such as malaria, sleeping sickness, leishmaniasis, and many others, the development of their treatment and prevention. Thanks to English as the main language at the conference and thanks to the growing interest of foreign colleagues, students and young scientists have the opportunity to establish valuable foreign cooperation with major world scientific institutions, often leading to lifelong friendships. We hope you fully enjoy all parts of the conference! On behalf of the organization committee, sincerely, Johana Rotterová 1 PROGRAM SCHEDULE Program Schedule ֎ Monday April 22nd, 2019 ֎ 16:00 REGISTRATION START 18:00 WELCOME RECEPTION ֎ Tuesday April 23rd, 2019 ֎ 8:00 BREAKFAST 8:50 Conference Opening Endosymbiotic Organelles Evolution פ Plenary lecture פ 9:00 COURTNEY STAIRS: Hacking the electron transport chain to live without oxygen PAWEŁ HAŁAKUC: How to find things that are lost – searching 10:00 for MROs in Preaxostyla VOJTĚCH VACEK: News in iron-sulphur cluster 10:20 assembly in oxymonads LAWRENCE RUDY CADENA: Revealing an interplay between the 10:40 MICOS complex and F1Fo-ATP synthase in Trypanosoma brucei 11:00 COFFEE BREAK 2 PROGRAM SCHEDULE Biodiversity, Phylogeny, and Systematics of Protozoa ALICJA FELLS: Cell in the shell – preliminary phylogenetic 11:20 analyses of the loricate taxa of Euglenids: Trachelomonas and Strombomonas ALEXEI KOSTYGOV: Insights into the evolution of life 11:40 cycles in the genus Phytomonas KATEŘINA POLÁKOVÁ: Anaerobic scuticociliates: A diverse ciliate 12:00 lineage hosting symbiotic prokaryotes 12:20 LUNCH Cell Biology of Protozoa 13:40 DOMINIK ARBON: Adaptive utilization of iron in Naegleria fowleri RONALD MALYCH: Proteins interacting with oxygen 14:00 and its reactive species in Naegleria gruberi VLADIMÍRA NAJDROVÁ: The guided entry of tail-anchored 14:20 proteins pathway in Giardia intestinalis VLADIMÍR VARGA: A PCR only tagging-based system for inducible 14:40 overexpression of proteins from endogenous loci in Trypanosoma brucei ZDENĚK VERNER: Peroxisomes and Entamoeba histolytica: 15:00 To be or not to be? 15:20 COFFEE BREAK Genomics ZOLTÁN FÜSSY: Subcellular compartments interplay for carbon 15:40 and nitrogen allocation in Chromera velia and Vitrella brassicaformis AGNIESZKA PEŁESZ: Genome sequencing of the putatively 16:00 free-living diplomonad Trimitus sp. IT1 3 PROGRAM SCHEDULE TEREZA ŃEVČÍKOVÁ: Plastid genomes and proteins illuminate 16:20 the evolution of eustigmatophyte algae and their bacterial endosymbionts MICHAŁ KARLICKI: Metagenomic insight into eukaryotic 16:40 picoplanton community of the Baltic Sea DAVID ŅIHALA: Evolution and unprecedented variants of the 17:00 mitochondrial genetic code in a lineage of green algae 17:20 POSTER SESSION 18:20 DINNER 20:00 CPS MEETING ֎ Wednesday April 24th, 2019 ֎ 8:00 BREAKFAST Endosymbiotic Organelles Evolution פ Plenary lecture פ ANDREW ROGER: Coevolution of genomes, organelles 9:00 and endosymbionts in anaerobic protists LUKÁŃ V. F. NOVÁK: Comparative genomics of Preaxostyla 10:00 flagellates: insights into the evolution of amitochondriality ROMANA PETRŅELKOVÁ: Unveiling mitochondrion-related 10:20 organelles in caviomonads JOHANA ROTTEROVÁ: Where oxygen is not popular 10:40 – anaerobiosis in SAL super-group (Ciliophora) 4 PROGRAM SCHEDULE 11:00 COFFEE BREAK Molecular Biology of Protozoa MICHAELA KUNZOVÁ: Reactive oxygen species as signalling 11:20 molecules involved in RBP6 induced in vitro differentiation of Trypanosoma brucei ANSGAR GRUBER: Interplay and possible interaction 11:40 of mitochondria and nuclei in a bicosoecid JAN PYRIH: Mitochondria of some protist lineages have retained 12:00 the bacterial signal peptide recognition particle-based protein targeting machinery 12:20 LUNCH Molecular Biology of Protozoa JULIUS LUKEŃ: Loss of the haptoglobin-haemoglobin receptor 13:40 in Trypanosoma brucei blocks life-cycle differentiation CLARETTA BIANCHI: New species of monoxenous 14:00 trypanosomatids with catalase-encoding gene MICHALA BOUDOVÁ: The role of Q-tRNA modification 14:20 in the development of Leishmania mexicana ALEJANDRO JIMÉNEZ-GONZÁLEZ: How do I survive here? 14:40 Evolution of the metabolic and detoxification pathways in diplomonads ANZHELIKA BUTENKO: Probable endosymbiotic gene transfer 15:00 in Euglenozoa and the origin of glycosome 15:20 AFTERNOON PROGRAM – SEE WEBSITE 18:20 BANQUET 20:20 PARTY 5 PROGRAM SCHEDULE ֎ Thursday April 25th, 2019 ֎ 8:00 BREAKFAST Endosymbiotic Organelles Evolution פ Plenary lecture פ 9:00 ANNA KARNKOWSKA: Exploring microbial eukaryotes to understand the evolution of the endosymbiotic organelles KACPER MACISZEWSKI: Missing genomes, unusual structures 10:00 and other oddities: insights into the evolution of plastid genomes of Euglenophyta ANNA NOVÁK VANCLOVÁ: Novel proteins and reorganized 10:20 protein import in the secondary plastid of Euglena gracilis PETR SOUKAL: Gene transfer accompanying the secondary 10:40 endosymbiosis of euglenid plastid 11:00 COFFEE BREAK Biodiversity, Phylogeny, and Systematics of Protozoa ILYA UDALOV: Diversity of Fornicates in the view of environmental 11:20 V4 and V9 datasets: contrasting taxonomic composition and putative new clades MONIKA WISNIEWSKA: Phylogenomic analyses of ten 11:40 transcriptomes of secondarily free-living diplomonads ANNA GANYUKOVA: Angomonas deanei (Trypanosomatidae) life 12:00 cycle in intestines of two fly species: Calliphora vicila and Lucilia sericata (Brachycera, Calliphoridae) 12:20 LUNCH 6 PROGRAM SCHEDULE Cell Biology of Protozoa JANA PILÁTOVÁ: The purine microcrystals – particularly guanine 13:40 and uric acid – are commonly present in all main groups of protists serving as the N-rich depots INGRID SVERÁKOVÁ: Paramylon synthesis in Diplonema 14:00 papillatum HANA VÁCHOVÁ: Studying of the processes at the ciliary 14:20 tip of eukaryotic cilium 14:40 LUBOŃ VOLEMAN: Mitosomal dynamics in Giardia intestinalis ALŅBĚTA MOTYČKOVÁ: How does ISC system work 15:00 in the mitosomes of Giardia intestinalis? 15:20 COFFEE BREAK Molecular Biology of Protozoa LUCIA HADARIOVÁ: Lipid analyses of E. gracilis cells, organelles 15:40 and plastid subfractions sheds light on the origin of plastid membranes KATEŘINA ŅENÍŃKOVÁ: Copper metabolism of Naegleria gruberi 16:00 and Naegleria fowleri 16:20 DANIEL SOJKA: Targetable proteolytic events in Babesia TAMARA SMUTNÁ: Multidomain flavodiiron protein 16:40 from Trichomonas vaginalis cytosol VENDULA RAŃKOVÁ: ZapE/Afg1 is Oxa1-dependent regulator 17:00 of respiratory complexes in Trypanosoma brucei TOMÁŃ PÁNEK: Dynamin-related GTPases involved in cristae 17:20 remodelling and their evolution VOJTĚCH ŅÁRSKÝ: Mastigamoeba balamuthi 17:40 and preadaptations to parasitism of Entamoeba 7 PROGRAM SCHEDULE 18:00 DINNER 19:20 CONFERENCE ANNOUNCEMENTS 19:40 DEMONSTRATION OF PROTISTS ֎ Friday April 26th, 2019 ֎ 8:00 BREAKFAST Friday symposium פ Plenary lecture פ 9:00 MATTHEW BROWN: Between a pod and a hard test: Deep evolution and development of amoeboid microbes 10:00 WORKSHOP I – Alex Tice 11:00 COFFEE BREAK 11:20 WORKSHOP II – Alex Tice 12:20 LUNCH 13:40 WORKSHOP III – Serafim Nenarokov 14:40 DEPARTURE 8 ABSTRACTS Abstracts Adaptive utilization of iron in Naegleria fowleri Dominik Arbon1, Jan Mach1, Kateřina Ņeníńková1, Maria Grechnikova1, Ronald Malych1, Róbert Ńuťák1 1. Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Czech Republic Iron is a vital trace element for all known organisms. Its significance lies in its attribute to convert between different oxidation states, thus being able to facilitate a number of redox reactions. The availability of iron is known to be, in some cases, the limiting factor of growth in certain habitats. This phenomenon is even further augmented in the environment of another living organism, which is the case of pathogens and parasites. Naegleria fowleri is a human pathogen, the causative agent of the fatal disease primary amoebic meningoencephalitis (PAM). This condition is marked by the fast onset of symptoms such as fever, nausea, vomiting, followed by coma and death in a span of only a few weeks. Treatment is complicated and not very successful, the fatality rate is above 97%. The pathogen is found all around