Worm Breeder’s Gazette 16(5) Table of Contents Worm Breeder’s Gazette 16(5) Table of Contents Reminder of the 13th International C. elegans Meeting. [p 3] Paul Sternberg 2001 Genetic Map of C. elegans: Call for Data. [p 4] Jonathan Hodgkin and Sylvia Martinelli Practical Course Announcement: 31st Wellcome Trust Advanced Course. [p 5] Jonathan Hodgkin, Alan Coulson and Patricia Kuwabara Ver midi IV. [p 6] Nathalie Pujol and Jonathan Ewbank Center for C. elegans Anatomy Expands its Archives. [p 7] David Hall Postdoctoral Position Available. [p 8] Eli Y. Adashi CGC Bibliographic References. [p 9] Theresa Stiernagle and Bob Herman Improved Survivorship of Frozen C. elegans Deletion Mutant Libraries. [p 31] Andrew M. Beld, David M. Miller and Kevin Strange Mapping Useful GFP Insertions: Evidence for Local Suppression of Recombination. [p 33] Jonathan Hodgkin, Alan Coulson, Patricia Kuwabara and Participants in WTAC24 and WTAC27 On the Reproducibility of Large-Scale RNAi Screens. [p 35] Fabio Piano, Aaron Schetter, Diane Morton, Kris Gunsalus, Valerie Reinke, Stuart Kim and Ken Kemphues N2(ancestral) Is a Mutant with Reduced Fertility and Longevity. [p 37] Diana McCulloch and David Gems Tight-Seal Whole-Cell Patch Clamping of C. elegans Neurons: Erratum. [p 38] Shawn Lockery and Miriam Goodman Thiabendazole (with Dimethyl Sulfoxide and Methylene Blue) as a Possible Aid in the "Limited Contamination" Culture of Caenorhabditis elegans. [p 39] William C. Campbell Using Caenorhabditis elegans as a Model to Study Autophagy. [p 41] Willisa Liou Characterization of rcn-1, a Cacipressin Homologue in C. elegans. [p 44] Jin Lee, Jaya Bandyopadhyay, Tami Kingsbury, Kyle Cunningham and Joohong Ahnn Microevolution of Vulval Cell Lineages within Two Nematode Genera: Caenorhabditis and Oscheius. [p 45] Marie Delattre and Marie-Anne Felix Promoter Dissection of C. elegans Hox Gene egl-5. [p 46] Yingqi Teng and Scott W. Emmons egl-5 Promoter Analysis Provides Clues Toward Understanding Anterior/Posterior Addressing at the Cis-Regulatory Level. [p 47] Lisa Girard and Paul Sternberg Reverse Genetic Approach to Identify Genes Required for Thermotaxis in C. elegans. [p 49] Hitoshi Inada, Hidetoshi Komatsu, Mikihiro Kosaki and Ikue Mori RNAi Screen and Functional Analysis of Genes Required for Germline Development. [p 50] Momoyo Hanazawa, Makoto Mochii, Naoto Ueno, Yuji Kohara and Yuichi Iino Monoclonal Antibody MH33 Recognizes a Gut-Specific Intermediate Filament. [p 51] Tetsunari Fukushige and Jim McGhee Transcript Specific RNAi Phenocopies Two Classes of mig-6 Mutant. [p 52] Takehiro Kawano and Joseph G. Culotti Identification of Genes Required for Ventralward Axon Guidance in C. elegans. [p 53] Kazuko Fujisawa and Joe Culotti All Available Mutations in flp-1 Also Delete daf-10 Sequences, Confounding Phenotype Interpretation. [p 54] Michael Ailion and James H. Thomas GLC-3: A Fipronil and BIDN-Sensitive, but Picrotoxinin-Insensitive, L-Glutamate-Gated Chloride Channel Subunit from Caenorhabditis elegans. [p 55] Lucy Horoszok, Valerie Raymond, David Sattelle and Adrien Wolstenholme VEGF-Like Receptors in C. elegans. [p 56] Gratien Dalpe, LiJia Zhang, Enza Colonna, Marina Tarsitano, Paolo Bazzicalupo, Tetsunari Fukushige, Jim McGhee, Joe Culotti and Graziella Persico 6 Feb 2001 16:25 1 Worm Breeder’s Gazette 16(5) Table of Contents smu-2 Affects the Alternative Splicing of unc-52 Pre-mRNA and Is Homologous to a Mammalian Spliceosome-Associated Protein. [p 58] Angela Spartz, Jocelyn Shaw and Robert Herman An Attempt to Slow Aging in C. elegans. 18. No Effect of Sodium Fusidine. [p 59] Vladimir V. Bakaev An Attempt to Slow Aging in C. elegans. 19. A Positive Effect of 1-Butylbiguanide Hydrochloride. [p 60] Vladimir V. Bakaev Author Index. [p 61] 6 Feb 2001 16:25 2 Reminder of the 13th International C. elegans Meeting Reminder of the 13th International C. elegans Meeting Paul Sternberg HHMI and Division of Biology, 156-29, Caltech, Pasadena, CA 91125 The 13th International C. elegans Meeting will be held June 22-26, 2001 at The University of California, Los Angeles, which is located in the Westwood section of Los Angeles. Members of the Organizing Committee for this meeting are Paul Sternberg (chair), Susan Strome, Ralf Schnabel, Monica Driscoll, Jim Thomas, Jocelyn Shaw and Alex van der Bliek. The format of the meeting will be different from that of previous C. elegans meetings to accommodate the growth of the field. The mornings will have two-hour parallel platform sessions and one-hour workshops. There will be afternoon poster sessions and evening plenary sessions. We plan to have four plenary sessions of oral presentations and three poster sessions. More people will be able to speak with this format. A pamphlet describing meeting details and how to register, submit abstracts, and request financial aid will be emailed to all Worm Breeder’s Gazette subscribers, and will be downloadable from WormBase (http://www.wormbase.org ). Abstract submission will be handled electronically at http://elegans.swmed.edu (thanks to Leon Avery!). Registration will be handled electronically at a site to be announced. The deadline for registration, submission of abstracts, and sending financial aid requests is April 5, 2001. 6 Feb 2001 16:25 3 2001 Genetic Map of C. elegans : Call for Data 2001 Genetic Map of C. elegans : Call for Data Jonathan Hodgkin 1 , Sylvia Martinelli 2 1 Genetics Unit, Dept of Biochemistry, University of Oxford, Oxford OX1 3QU, UK 2 The Sanger Centre, Wellcome Trust Genome Campus, Hinxton Hall, Hinxton, Cambridge CB10 1SA, UK The deadline for new genetic map data to be incorporated into the 2001 Genetic Map of Caenorhabditis elegans (Printed Version) is 31 May 2001. As usual, the 2001 Map will be distributed as the last issue of this volume (Volume 16) of the Worm Breeders Gazette. This will also include lists of registered laboratories, gene names, genes, rearrangements and balancers. Genetic map data, as well as information on gene/sequence links, new loci, polymorphisms and rearrangements, and gene name proposals can be communicated to the CGC via the Sanger Centre website: http://www.sanger.ac.uk/Projects/C_elegans/CGC/ In particular, please note that proposed new gene names should be registered with the CGC before publication. This helps to ensure conformity with standard nomenclature, to avoid duplicated, unnecessary or inappropriate gene names, and to update the relevant databases. A summary of current recommendations for genetic nomenclature in Caenorhabditis elegans can be found at: http://elegans.swmed.edu/Genome/nomen.html Data can also be sent by e-mail to [email protected], or by fax or mail to J. Hodgkin. For large datasets, this may be a more convenient method of submission than the website. Standard forms for data submission are available by e-mail or fax on request to: Jonathan Hodgkin Genetics Unit, Dept of Biochemistry, University of Oxford, Oxford OX1 3QU, UK e-mail: [email protected] Fax: (+44) 1865 275318 6 Feb 2001 16:25 4 PRACTICAL COURSE ANNOUNCEMENT: 31st WELLCOME TRUST ADVANCED COURSE PRACTICAL COURSE ANNOUNCEMENT: 31st WELLCOME TRUST ADVANCED COURSE Jonathan Hodgkin 1 , Alan Coulson 2 , Patricia Kuwabara 2 1 Genetics Unit, Dept of Biochemistry, University of Oxford, Oxford OX1 3QU, UK 2 The Sanger Centre, Wellcome Trust Genome Campus, Hinxton Hall, Hinxton, Cambridge CB10 1SA, UK A practical course on Molecular, Genetic and Informatic Methods for C. elegans will take place at the Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, England, from Monday 10th September -- Tuesday 18th September 2001. Course instructors will be Alan Coulson, Jonathan Hodgkin and Patricia Kuwabara. The course will be similar in format and content to the 27th Wellcome Trust Advanced Course, which was held at the Sanger Centre in September 2000, with the same instructors. For further details, see the Wellcome Trust Courses website (http://www.wellcome.ac.uk/en/1/biosersss.html). Application deadline: 22nd June 2001 6 Feb 2001 16:25 5 Ver midi IV Ver midi IV Nathalie Pujol 1 , Jonathan Ewbank 2 1 IBDM, Marseille 2 CIML, Marseille La quatrième réunion annuelle des équipes françaises ayant un intérêt pour C. elegans se tiendra le Vendredi 23 février 2001 à Luminy, Marseille. Contact: [email protected] [email protected] 6 Feb 2001 16:25 6 Center For C. elegans Anatomy Expands its Archives Center For C. elegans Anatomy Expands its Archives David Hall Albert Einstein College of Medicine Bronx, NY 10461 We have recently received thousands of archival prints from the collections of the MRC-LMB in Cambridge and from the White lab in Madison. When added to the extensive collections we have received from the former Russell lab, and from our own files, we now have a staggering number of prints and negatives. Over the next few years we will scan selected prints from wild type and mutant animals into digital format, including the original annotations used in analysing and reconstructing the various tissues. We expect to make some digital data available to the community by the time of the International meeting in late June 2001. Due to bandwidth restrictions, we expect to write the data onto either CDs or DVDs for now, as the large file sizes required for the images will make it difficult to transfer files over the web. John White has retained archival prints covering the region of the vulva in Madison, but virtually all the rest of the MRC-LMB collection is now stored here in New York. We are very appreciative of the consideration of both John White and Jonathan Hodgkin in choosing us as the new repository for this collection. We welcome visitors to visit our laboratory if they wish to view the data in the print archive. Directions on how to reach us are listed on our website.