Melan-A Antibody [MLANA/788] Cat. No.: 33-360 Melan-A Antibody [MLANA/788] Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A antibody (MLANA/788). Specifications HOST SPECIES: Mouse SPECIES REACTIVITY: Dog, Human, Mouse, Rat Recombinant human MLANA protein was used as the immunogen for the Melan-A IMMUNOGEN: antibody. TESTED APPLICATIONS: Flow, IF, IHC-P, WB September 26, 2021 1 https://www.prosci-inc.com/melan-a-antibody-mlana-788-33-360.html Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Western blot: 0.5-1 ug/ml APPLICATIONS: Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the Melan-A antibody should be determined by the researcher. 1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Properties PURIFICATION: Protein G affinity chromatography CLONALITY: Monoclonal ISOTYPE: IgG1 CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide CONCENTRATION: 0.2 mg/mL STORAGE CONDITIONS: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. Additional Info OFFICIAL SYMBOL: MLANA ALTERNATE NAMES: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1 GENE ID: 2315 USER NOTE: Optimal dilutions for each application to be determined by the researcher Background and References This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous BACKGROUND: cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. September 26, 2021 2 https://www.prosci-inc.com/melan-a-antibody-mlana-788-33-360.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/melan-a-antibody-mlana-788-33-360.html.