Leader in Biomolecular Solutions for Life Science Phospho-Histone H3-T11 Rabbit pAb Catalog No.: AP0361 Basic Information Background Catalog No. Histones are basic nuclear proteins that are responsible for the nucleosome structure of AP0361 the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is Observed MW wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with 15kDa linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent Calculated MW histone that is a member of the histone H3 family. Transcripts from this gene lack polyA 15kDa tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. Category Primary antibody Applications WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 8356 P68431 Immunogen A phospho specific peptide corresponding to residues surrounding T11 of human HIST1H3J Synonyms HIST1H3J;H3/j;H3FJ Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts from U-87MG cells, using Phospho-Histone H3-T11 Antibody (AP0361).U-87MG cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum- starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..