DSM 2016, Kyoto BOOK OF ABSTRACTS Sampling at the deepest bottom, Mariana Trench Challenger Deep (11,000 m) 5th International Workshop on Deep-Sea Microbiology September 10-11, 2016, Kyoto, Japan ・This workshop is supported by The Kyoto University Foundation. ・This program is supported by a subsidy from Kyoto City and the Kyoto Convention & Visitors Bureau. 1 DSM 2016, Kyoto 5th International Workshop on Deep-Sea Microbiology September 10-11, 2016, Kyoto, Japan The aim of the workshop is to gather international experts in the field of deep-sea microbiology, and provide the participants the opportunity to present very recent data, and to discuss future cooperative works, in a friendly atmosphere. Oral presentations are basically made by selected scientists, but the meeting is open to a broad audience including PhD students and Post-docs. This will give young scientists the opportunity to listen to up to date talks, and meet and discuss with experts during the breaks. Just after this workshop, you can join the Extremophiles 2016 Kyoto congress (Sep. 12-16, http://www.acplan.jp/extremophiles2016/). During the Extremophiles Conference held in Brest, France, in September 2006, the idea to hold a series of workshops dedicated to deep-sea microbiology was launched. Prof. Dr. Xiao Xiang (University of Shanghai, China) took the job and organized the first workshop in Xiamen, China, in November 2008, where he was settled at that time. The meeting was a great success and encouraged the organization of further workshops. The second WS was organized by Prof. Daniel Prieur (University of Brest, France) in September 2010 in Brest, France. The 3rd WS was organized again by Prof. Xiao Xiang, and held in Shanghai, China, in October 2012. The 4th WS was in Brest, France, in September 2014, and organized by Prof. Dr. Mohamed Jebbar (CNRS-Ifremer-UBO, France). The time has come to schedule the 5th WS in Kyoto, Japan, which will be held in September 2016. Cooperated by The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) KANEKA Co. The Dreaming Company Syn Corporation Bioengineering Lab. Co. WDB Environmental & Biological Research Institute Organizing Committee Local Committee: Satoshi Nakagawa (Chair, Kyoto University) Ken Takai (JAMSTEC) Chiaki Kato (JAMSTEC) Haruyuki Atomi (Kyoto University) Takuro Nunoura (JAMSTEC) Scientific advisory board: Mohamed Jebbar (CNRS-Ifremer-UBO, France) Anne Godfroy (Ifremer, France) Douglas H. Bartlett (SIO, UCSD, USA) Anna-Louise Reysenbach (Portland University, USA) Xiang Xiao (SJTU, China) Ken Takai (JAMSTEC, Japan) Office Laboratory of Marine Environmental Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Kyoto 606-8502, Japan E-mail: [email protected] URL: http://www.kanbi.marine.kais.kyoto-u.ac.jp/Site/Workshop2016/Top.html 2 DSM 2016, Kyoto General information About the Venue: Maskawa Hall, located in the North Campus, Kyoto University It’s 5-10 min walk from the venue of Extremophiles 2016 (September 12-16 @ Kyoto University Clock Tower Centennial Hall). About Kyoto: Kyoto is often called "Japan's heartland". Over a period of 1200 years, dating from the decision to move the capital to Kyoto in 794, it nurtured a splendid, delicate and unique kind of culture, and over the course of history came to be considered the mother of culture within Japan. Natural scenery, temples, shrines, towns and homes intermingle with a poignant historical beauty. Whether it is the Tea Ceremony or Japanese flower arrangement or Nishijin-brocade, so many aspects of characteristic Japanese culture continue to thrive in Kyoto. For more information, see websites of travel+leisure and Kyoto city. Kyoto Station 3 DSM 2016, Kyoto Accommodation & Travel Hotels There are lots of hotels available in Kyoto. But, Kyoto is a very popular destination, so it is advised to book AS EARLY AS POSSIBLE (right now if you can). This is pretty important!! Travel From airport From Tokyo, (via Bullet train) 4 DSM 2016, Kyoto From Kyoto station Kyoto-city Guide Map See the following pdf → guidemap.pdf Instruction for oral presentation You can use your own laptop computer. Please make sure that your PC has a VGA output. VGA connector is a three-row 15-pin DE-15 connector (Mini D-sub-15pin). Our computer (Mac OS10.8.5) is also available. PowerPoint (ver 2011) and Key Note (ver 5.3) are installed. All speakers are requested to preview their presentation files during a coffee or lunch break before the start of each session. Presenting allotted time is 20-30 min including discussion and change of speakers, speakers are kindly asked to keep the time of their presentations. Instruction of poster presentation Poster size could be about A0 size (84 cm x 120 cm). The WS office will provide the pin and tapes for fix the poster on board. Poster presenters should set the poster on Sep. 10, morning, and take out on Sep. 11, evening. 5 DSM 2016, Kyoto Scientific program Saturday, 10 September 2016 8:30 - Registration Desk Open 9:05 - 9:10 Opening address Workshop Chair: Satoshi Nakagawa (Kyoto Univ.) Session 1. ・・・・・・・・・・・・・・・・・・・・・・ 9 Session Chair: Chiaki Kato (JAMSTEC) 9:10 - 9:50 O-1 Mohamed Jebbar (Univ. Brest, CNRS, Ifremer) Adaptation to high hydrostatic pressure in hyperthermophilic piezophiles from deep-sea hydrothermal vents. 9:50 -10:20 O-2 Anais Cario (Rensselaer Polytechnic Institute) Experimental high-pressure cultivation with the PUSH50: increased pressure tolerance of a marine bacterium without subsampling decompression. 10:20 - 10:50 Coffee break & Poster session Session 2. ・・・・・・・・・・・・・・・・・・・・・・ 11 Session Chair: Mohamed Jebbar (Univ. Brest, CNRS, Ifremer) 10:50 - 11:20 O-3 André Antunes (Edge Hill University) Extreme biology in the deeps of the Red Sea. 11:20 - 11:40 O-4 Xiang ZENG (Third Institute of Oceanography, SOA) Ferric iron reduction by a piezophilic thermophilic fermentative bacterium Anoxybacter fermentans strain DY22613T from deep-sea hydrothermal vents. 11:40 – 12:10 O-5 Eva Sintes (University of Vienna) The role of sulfur sources for deep ocean microorganisms. 12:10 - 14:00 Lunch (not provided) Session 3. ・・・・・・・・・・・・・・・・・・・・・・ 13 Session Chair: Takuro Nunoura (JAMSTEC) 14:00 - 14:40 O-6 Long-Fei Wu (CNRS-CAS) Occurrence and characteristics of magnetotactic bacteria in seamount habitats. 14:40 - 15:10 O-7 Weijia Zhang (Ins. Deep-Sea Sci. Eng., SOA) Contribution of TMAO to the high-hydrostatic pressure adaptation of deep-sea bacteria. 15:10 - 15:40 O-8 Masayoshi Nishiyama (Kyoto Univ.) Microscopic analysis of the swimming motility of deep-sea bacteria at high pressure. 15:40 - 16:10 O-9 Jun Kawamoto (Kyoto Univ.) Physiological function of polyunsaturated fatty acids in microbial cold and high-pressure adaptation. 16:10 - 16:40 Coffee break & Poster session Session 4. ・・・・・・・・・・・・・・・・・・・・・・ 16 Session Chair: Long-Fei Wu (CNRS-CAS) 16:40 - 17:00 O-10 Chiaki Kato (JAMSTEC) Microbial diversity of the intestine from the deep-sea sharks and isolation of the Photobacterium sp. 17:00 - 17:20 O-11 Sotaro Fujii (Hiroshima Univ.) Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea 17:20 - 17:50 O-12 Shunsuke Sato (Kaneka Co.) Environment compatible KANEKA biopolymer AONILEX®. 18:45 - 21:00 Dinner (Fortune garden; applicant only) →URL: http://www.fortunegarden.com/en/ 6 DSM 2016, Kyoto Sunday, 11 September 2016 Session 5. ・・・・・・・・・・・・・・・・・・・・・・ 18 Session Chair: Peter R. Girguis (Harvard Univ.) 9:00 - 9:30 O-13 So Fujiyoshi (Kyoto Univ.) Unique morphological features of deep-sea endemic crab ventral setae. 9:30 - 10:00 O-14 Yong Wang (Ins. Deep-Sea Sci. Eng., SOA) Genomic characterization of mycoplasma symbiotic bacteria from the stomach of deep-sea isopod Bathynomus sp. 10:00 -10:30 Coffee break & Poster session Session 6. ・・・・・・・・・・・・・・・・・・・・・・ 19 Session Chair: Elizaveta Bonch-Osmolovskaya (Winogradsky Ins. Microbiol., RAS) 10:30 - 11:10 O-15 Peter R. Girguis (Harvard Univ.) Short, fat, skinny or tall: Rates and mechanisms of carbon fixation among siboglonid tubeworms at hydrothermal vents. 11:10 - 11:40 O-16 Masahiro Yamamoto (JAMSTEC) Electro-ecosystem in deep-sea hydrothermal fields. 11:40 - 12:00 O-17 Cécile Dalmasso (Univ. Brest, CNRS, Ifremer) Thermococcus piezophilus sp. nov., an hyperthermophilic archaeon with a broad pressure range for growth, isolated from the Mid-Cayman Rise. 12:00 - 14:00 Lunch (not provided) Session 7. ・・・・・・・・・・・・・・・・・・・・・・ 21 Session Chair: Satoshi Nakagawa (Kyoto Univ.) 14:00 - 14:30 O-18 Brett J. Baker (Univ. Texas) Genomics uncovers the functional potential of microbial communities in deep-sea hydrothermal vent sediments. 14:30 - 15:00 O-19 Shingo Kato (JAMSTEC) Metabolic potential of uncultured bacteria in massive sulfide deposits below the deep seafloor revealed by metagenomic analyses. 15:00 - 15:30 O-20 Mercier Coraline (UBO) Discovery of bacteriophages amongst the order of Thermotogales. 15:30 - 16:00 O-21 Daniel De Corte (JAMSTEC, Univ. Vienna) Large-scale distribution of microbial and viral populations in the South Atlantic Ocean 16:00 - 16:30 Coffee break & Poster session Session 8. ・・・・・・・・・・・・・・・・・・・・・・ 24 Session Chair: Ken Takai (JAMSTEC) 16:30 - 17:00 O-22 Tomoyo Okumura (JAMSTEC) Brucite-carbonate chimneys discovered in the Shinkai Seep Field, a serpentinite-hosted vent system in the Southern Mariana Forearc. 17:00 - 17:30 O-23 Sayaka Mino (Hokkaido Univ.) Population genetics and phenotypic differences of cosmopolitan mesophilic Sulfurimonas at deep-sea hydrothermal vents. 17:30 - 18:00 O-24 Elizaveta Bonch-Osmolovskaya (Winogradsky Ins. Microbiol., RAS) New catabolic processes that can fuel anaerobic deep-sea hydrothermal ecosystems. 18:00 - 18:05 Closing remarks Ken Takai (JAMSTEC) 7 DSM 2016, Kyoto Poster ・・・・・・・・・・・・・・・・・・・・・・ 26 P-1 Xuegong Li (Ins. Deep-Sea Sci. Eng., SOA) Genomic analysis of an extremely piezophilic Shewanella benthica DB21MT-2 isolated from the Mariana Trench. P-2 Qunjian Yin (Ins. Deep-Sea Sci.