Shivaji University , Kolhapur Seat Paper Code(ENT202015) No. M.Sc Entrance Exam , MAY-2020 58717-MICROBIOLOGY Day And Date : Friday 20/11/2020 Total Marks : 100 Time : 13:00 To 14:30 Marks 1 Available water value for pure water is -----------. 1 A) 1.00 B) 9.99 C) 0.98 D) 0.1 2 LAL test is used for ------------testing. 1 A) pyrogen B) carcinogenicity C) allergy D) toxicity 3 -------------- causes potato spindle Tuber disease. 1 A) Viroid B) TMV C) Prion D) HIV 4 Mycorrhiza is a symbiotic association between -------------. 1 A) algae & fungi B) plant roots & fungi C) bacteria & fungi D) Plant roots & bacteria 5 In carbon cycle, autotrophic bacteria fix CO2 mainly by ---------. 1 A) Calvin-Benson cycle B) Glyoxylate cycle C) EMP pathway D) PP pathway 6 The organic matter usually contributes ----------% of the total volume of the soil. 1 A) 1-2 B) 3-6 C) 10-15 D) 20-30 7 Farm yard manure is prepared by using -----------. 1 A) City waste B) Industrial waste C) Agricultural waste D) Domestic waste 8 For optimal composting, C:N ratio of waste should be ----------. 1 A) 20:1-4O:1 B) 10:1-15:1 C) 5:1-10:1 D) 1:1-5:1 9 ---------- is a non-nodulating nitrogen fixer used to prepare biofertilizer. 1 A) Frankia B) Rhizobium C) Azospirillum D) Klebsiella 10 Insecticidal property of Bacillus thuringiensis is due to --------gene in the genome. 1 A) Lux B) Cry C) Col D) Nif 11 Enzyme ------- attacks native cellulose molecule. 1 A) Beta glucosidase B) C 1 C) C x D) Glycosidase 12 --------- transmission is an example of direct transmission of plant pathogens. 1 A) Germinative B) Water C) Wind D) Animal 13 Staphylococcal enterotoxin ----------- is more heat resistant. 1 A) A B) B C) C D) D 14 ----------broth medium is used for production of rhizobial inoculants, 1 A) Ashby’s B) Jensen’s C) Okon’s D) Yeast extract mannitol 15 Lineweaver-Burk plot is called as---------- 1 A) Michaelis and Menten plot B) Approximate plot C) Inverted plot D) Double reciprocal plot 16 Turnover number is denoted by-------- 1 A) Vmax B) K1 C) Kcat D) Km 17 Ribozyme is……. 1 A) RNA without phosphate B) RNA with sugar C) RNA with extra phosphate D) RNA with enzyme activity 18 In acid base catalysis, acids temporarily ….....a proton 1 A) Accept B) Retain C) Donate D) Reject 19 Electrophoresis of enzymes is based on------------ 1 A) Size of enzymes B) Heat sensitivity of enzymes C) Solubility of enzymes D) Adsorption of enzymes 20 The clover leaf model of t-RNA was proposed by -------- 1 A) Kornberg B) Holley C) Ehrlich D) Jacob 21 ……. is the major enzyme involved in Glyoxylate pathway 1 A) Isocitrate dehydrogenase B) Isocitrate dehydratase C) Isocitrate lyase D) Isocitrate decarboxylase 22 The most widely used inorganic salt in salt precipitation process of enzyme 1 purification is ……. A) ammonium phosphate B) ammonium sulfate C) ammonium nitrate D) ammonium chloride 23 ………. is the first immobilized enzyme 1 A) lipase B) lyase C) invertase D) isomerase 24 Temperature of liquid nitrogen for storage of industrial cultures is ---------oC. 1 A) -120 B) -170 C) -196 D) -96 25 The valency of sulfur in the form of sulfate,S04 is……… 1 A) 6 B) 4 C) 2 D) 3 26 Interferon induces--------- enzyme that cleaves the terminal 3 –CCA sequence from 1 tRNA. A) phosphodiesterase B) protein kinase. C) RNase L D) 2,5 (A) Synthetase. 27 In monoclonal antibody technology, tumor cells that can replicate endlessly are 1 fused with mammalian cells that produce an antibody. The result of this cell fusion is a ----------- A) lymphoblast B) hybridoma C) myeloma D) hybrid 28 The altered Fc portion enables IgE to bind to glycoprotein receptors of ------------- 1 A) eosinophils B) neutrophils C) basophiles D) macrophages 29 Majority of T cells contain --------type of T cell receptor 1 A) γδ B) αβ C) µγ D) αγ 30 Endogenous antigens are processed by ------------ pathway in antigen presenting 1 cell. A) Cytosolic B) Glycolytic C) Endocytic D) Endoplasmic 31 ……… stem cells have the potential to develop into any cell found in the human 1 body but not the placenta. A) Totipotent B) Pluripotent C) multipotent D) unipotent 32 -------- is part of the mechanism for inducing central tolerance in B cells by clonal 1 deletion. A) The presence of soluble antigen B) The ability to undergo receptor editing C) The display of self-antigen in a membrane bound form D) Activity of functional autoimmune regulator gene. 33 In rheumatoid arthritis patients, the classic RA factor is nothing but ……. 1 A) IgM against collagen B) IgM against Fc region of IgG C) IgG against collagen D) IgG against Fc region of IgM 34 ........... reduces histamine level by blocking conversion of histidine to histamine. 1 A) Theophylline B) Cortisone C) Epinephrine D) Antihistamine 35 For production of grape wine, sugar concentration in grape must varies between -- 1 ------- %. A) 2-3 B) 5-6 C) 7-8 D) 3-4 36 In phagocytosis, reactive oxygen species are generated by --------------enzyme 1 complex in phagosome. A) oxygenase B) nitric oxide synthase C) NADPH phagosome oxidase D) dismutase 37 ------------ contains two identical copies of ‘plus’ sense RNA. 1 A) Adenovirus B) HIV C) TMV D) Influenza 38 During lysogenic interaction lambda phage genome gets integrated in between ---- 1 ------ genes of E. coli A) gal & bio B) Pro A & Pro B C) his & bio D) Pro & gal 39 ---------- genes of T4 phage codes for proteins involved in its DNA replication. 1 A) Immediate early B) immediate late C) Late D) Delayed early 40 Under optimum conditions latent period of T4 phage is --------------- min 1 A) 60 B) 123 C) 40 D) 25 41 Cancerous cells are -------------- cells. 1 A) Invasive & immortal B) Mortal & harmful C) Immortal & harmless D) Benign 42 Attenuation is mechanism of regulation of ____________ operon. 1 A) lactose B) arabinose C) tryptophan D) histidine 43 The method used for paternity determination is _________ 1 A) DNA sequencing B) DNA fingerprinting C) PCR D) Western blotting 44 The production of wild type phenotype by combining two genomes carrying 1 mutation in a diplod is called as ____________. A) Suppression B) Complementation C) Transformation D) Electroporation 45 ____________ bring about non genetic suppressor mutation 1 A) Acridine dyes B) Streptomycin C) Nitric acid D) Mustard gas 46 A key intermediate in the production of L-lysine is ------------- acid. 1 A) dipicolinic B) pyruvic C) acetic D) diaminopimelic 47 Dideoxynucleotide chain termination method is used for ___________. 1 A) DNA sequencing B) DNA fingerprinting C) DNA amplification D) DNA degradation 48 pBR 322 is derived from ____________. 1 A) RSF 2124, pMB 1 and pSC 101 B) RSF 101, pMB 1 and pTi C) RSF 01, pSC 101 and pRM 1 D) RSF 21, pTB 11 and pTi 49 Beadle and Tatum selected ____________ as an experimental organism. 1 A) Aspergillus niger B) Snapdragon C) Neurospora crassa D) Saccharomyces sp. 50 The vector pUC 19 possess ______________ gene. 1 A) lac Z B) lac Y C) lac A D) lac B 51 The end of phenotypic lag is the indication of formation of _________. 1 A) Mutant homokaryon B) Wild type homokaryon C) Mutant heterokaryon D) Wild type heterokaryon 52 Paramecium aurelia contain ____________ gene necessary for reproduction of 1 kappa particles. A) P B) K C) D D) N 53 --------------- form of DNA has 11 nucleotide base pairs per turn of helix. 1 A) D B) B C) Z D) A 54 Presence of ------------- protects the bacteria from phage infection. 1 A) flagella B) capsule C) pili D) cell wall 55 A refractive index of oil immersion oil is similar to ----------- 1 A) air B) water C) glass slide D) oil 56 ZNCF is a mixture of --------- 1 A) basic fuchsin and phenol B) phenol and congo red C) malachite green and basic fuchsin D) phenol and ethanol 57 Biogas contains ----------- % of methane. 1 A) 10-30 B) 30-50 C) 50-80 D) 80-90 58 In metachromatic granule staining, the stain ----------- colours volutin granules. 1 A) toluidine blue B) malachite green C) Albert’s iodine D) congo red 59 Scientist ------------- discovered that the causative agent of Tobacco Mosaic 1 Disease was filterable. A) Robert Koch B) Ivanowsky C) Beijerinck D) Loeffler 60 The existence of bacteria in thermolabile and thermostable forms was confirmed 1 by scientist --------------. A) John Tyndall B) Louis Pasteur C) Joseph Lister D) Robert Koch 61 In green and purple bacteria photosynthetic electron transport chain is located in 1 _________. A) Cell membrane B) Periplasmic space C) Thallakoid membrane D) Nuclear membrane 62 Facultative anaerobes use __________ as final electron acceptor. 1 A) Oxygen B) Nitrate C) Phosphate D) Nitrogen 63 __________ enzymes are believed to be essential enzymes. 1 A) Inducible B) Constitutive C) Extracellular D) Inducible and exoenzymes 64 Volutine granules are ....... in nature. 1 A) basophilic B) acidophilic C) neutral D) hydrophilic 65 Chemoreceptors are involved in ........process. 1 A) chemotaxis B) phototaxis C) aerotaxis D) magnetotaxis 66 The DNA polymerase involved in base excision repair is ____________ 1 A) DNA polymerase α B) DNA polymerase β C) DNA polymerase σ D) DNA polymerase γ 67 .......... is the function of enzyme involved in base excision repair. 1 A) Addition of correct base B) Addition of correct nucleotide C) Removal of incorrect base D) Removal of phosphodiester bond 68 Small laboratory fermenters for scale up studies are of ---------- liter capacity. 1 A) 1-10 B) 10-15 C) 15-20 D) 20-25 69 .........