Biographical Sketch Xiaoliang Sunney Xie Lee Shau Kee Professor Director, Beijing Advanced Innovation Center for Genomics, Peking University Director, Biomedical Pioneeing Inovation Center, Peking University Personal Statement: Xiaoliang Sunney Xie received a B.S. from Peking University in 1984, and his Ph.D. from the University of California at San Diego in 1990, followed by a short postdoctoral experience at the University of Chicago. In 1992, Xie joined Pacific Northwest National Laboratory, where he later became a Chief Scientist. In 1999, he was appointed Professor of Chemistry at Harvard University, and in 2009, Mallinckrodt Professor of Chemistry and Chemical Biology at Harvard. In 2018, he was appointed as the Lee Shau Kee Professor at Peking University. He currently serves as the Director of Biomedical Pioneeing Inovation Center (BIOPIC) and the Director of Beijing Advanced Innovation Center for Genomics. Xie has made major contributions to the emergence of the field of single-molecule biophysical chemistry and its application to biology. In particular, his group pioneered single-molecule enzymology and live cell studies of gene expression and regulation on a single molecule basis. He also led the development of coherent Raman scattering microscopy, enabling sensitive 3D imaging of live cells and organisms based on vibrational spectroscopy. In addition, his team developed methods for sequencing the genome of a single human cell with high precision, which has been applied to in vitro fertilization, allowing many newborns to avoid genetic disorders. His honors include two NIH Director's Pioneer Awards, American Chemical Society’s Harrison Howe Award and Peter Debye Award, Biophysical Society’s Founders Award, Department of Energy’s E.O. Lawrence Award in Chemistry, the Leibinger Innovation Prize, the Sackler Prize for Physical Sciences, and the Albany Prize in Medicine. Xie is a fellow of the American Academy of Arts and Sciences, a member of the US National Academy of Sciences, a member of the US National Academy of Medicine, and a foreign member of Chinese Academy of Sciences. Selected publications of the past five years: Tan, Longzhi; Xing, Dong; Chang, Chi-Han; Li, Heng; Xie, X. Sunney "Three-dimensional genome structures of single diploid human cells," Science 43, 924-928. DOI:10.1126/science.aat5641 (2018). Chen, Chongyi; Xing, Dong; Tan, Longzhi; Li, Heng; Zhou, Guangyu; Huang, Lei; Xie, X. Sunney "Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)," Science 356, 189-194. DOI:10.1126/science.aak9787 (2017). Yan, Liying; Huang, Lei; Xu, Liya; Huang, Jin; Ma, Fei; Zhu, Xiaohui; Tang, Yaqiong; Liu, Mingshan; Lian, Ying; Liu, Ping; Li, Rong; Lu, Sijia; Tang, Fuchou; Qiao, Jie; Xie, X Sunney "Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next- generation sequencing with linkage analyses," Proc Natl Acad Sci USA, 112(52), 15964-15969, DOI:10.1073/pnas.1523297113 (2015). Huang, Lei; Ma, Fei; Chapman, Alec; Lu, Sijia and Xie, X. Sunney "Single-Cell Whole-Genome Amplification and Sequencing: Methodology and Applications," Annu Rev Genomics Hum Genet 16:79-102, DOI:10.1146/annurev-genom-090413-025352 (2015). Xie, X. Sunney "Single Molecules Meet Genomics: Pinpointing Precision Medicine," JAMA, DOI:10.1001/jama.2015.5533 (2015). Ni, Xiaohui; Zhuo, Minglei; Su, Zhe; Duan, Jianchun; Gao, Yan; Wang, Zhijie; Zong, Chenghang; Bai, Hua; Chapman, Alec R.; Zhao, Jun; Xu, Liya; An, Tongtong; Ma, Qi; Wang, Yuyan; Wu, Meina; Sun, Yu; Wang, Shuhang; Li, Zhenxiang; Yang, Xiaodan; Yong, Jun; Su, Xiao-Dong; Lu, Youyong; Bai, Fan; Xie, X. Sunney; Wang, Jie. "Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients," PNAS 110, 21083-21088 DOI: 10.1073/pnas.1320659110 (2013). Hou, Yu; Fan, Wei; Yan, Liying; Li, Rong; Lian, Ying; Huang, Jin; Li, Jinsen; Xu, Liya; Tang, Fuchou; Xie, X. Sunney; Qiao, Jie. "Genome Analyses of Single Human Oocytes," Cell 155, 1492-1506 DOI:10.1016/j.cell.2013.11.040 (2013). Chong, Shasha; Chen, Chongyi; Ge, Hao; Xie, X. Sunney. "Mechanism of Transcriptional Bursting in Bacteria," Cell 158, 314-326 DOI:http://dx.doi.org/10.1016/j.cell.2014.05.038 (2014). Xie, X. Sunney. "Enzyme Kinetics, Past and Present," Science 342, 1457-1459 DOI:10.1126/science.1248859 (2013). Kim, Sangjin; Broströmer, Erik; Xing, Dong; Jin, Jianshi; Chong, Shasha; Ge, Hao; Wang, Siyuan; Gu, Chan; Yang, Lijiang; Gao, Yi Qin; Su, Xiao-dong; Sun, Yujie; Xie, X. Sunney. "Probing Allostery Through DNA," Science 339, 816-819 (2013). .