MED28 Antibody Catalog # ASC10737

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Specification

MED28 Antibody - Product Information

Application WB, IHC Primary Accession Q9H204 Other Accession NP_079481, 52851391 Reactivity Human, Mouse Host Rabbit Clonality Polyclonal Isotype IgG Application Notes MED28 antibody can be used for detection of MED28 by Western blot at 1 µg/mL. Antibody can also be used Western blot analysis of MED28 in human for immunohistoc brain tissue lysate with MED28 antibody at 1 hemistry starting µg/mL in (A) the absence and (B) the at 2.5 µg/mL. For presence of blocking peptide. immunofluoresce nce start at 20 µg/mL.

MED28 Antibody - Additional Information

Gene ID 80306 Target/Specificity MED28;

Reconstitution & Storage MED28 antibody can be stored at 4℃ for Immunohistochemistry of MED28 in human three months and -20℃, stable for up to brain tissue with MED28 antibody at 2.5 one year. As with all antibodies care should µg/mL. be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. MED28 Antibody - Background

Precautions MED28 Antibody: The mediator complex is a MED28 Antibody is for research use only multi-protein transcriptional co-activator that is and not for use in diagnostic or therapeutic expressed ubiquitously in eukaryotes from procedures. yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the MED28 Antibody - Protein Information proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a Name MED28 down-regulation of MED28 expression in

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Synonyms EG1 NIH3T3 cells results in a significant induction of several genes associated with smooth muscle Function cell differentiation and conversely its Component of the Mediator complex, a over-expression represses expression of SMC coactivator involved in the regulated genes. MED28 can also form a ternary complex transcription of nearly all RNA polymerase with Grb2 and Merlin, the neurofibromatosis 2 II-dependent genes. Mediator functions as a tumor suppressor protein, indicating that bridge to convey information from gene- MED28 may play a role in Merlin's tumor specific regulatory proteins to the basal suppressive activity. MED28 has also been RNA polymerase II transcription machinery. recently identified as an HIV dependency factor Mediator is recruited to promoters by direct (HDF), suggesting that MED28 may be an interactions with regulatory proteins and important drug target in HIV treatment. At serves as a scaffold for the assembly of a least two isoforms of MED28 are known to functional preinitiation complex with RNA exist. polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in MED28 Antibody - References cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling Sato S, Tomomori-Sato C, Parmely TJ, et al. A pathways. set of consensus mammalian mediator subunits identified by multidimensional protein Cellular Location identification technology. Mol. Cell2004; Nucleus. Cytoplasm. Membrane; Peripheral 14:685-91. membrane protein Note=According to Beyer KS, Beauchamp RL, Lee MF, et al. PubMed:15467741, it is cytoplasmic and Mediator subunit MED28 (Magicin) is a mainly membrane-associated repressor of smooth muscle cell differentiation. J. Biol. Chem.2007; 282:32152-7. Tissue Location Wiederhold T, Lee MF, James M, et al. Magicin, Widely expressed. Highly expressed in a novel cytoskeletal protein associates with the vascular tissues such as placenta, testis NF2 tumor suppressor merlin and Grb2. and liver Oncogene2004; 23:8815-25. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV MED28 Antibody - Protocols infection through a functional genomic screen. Science2008; 319:921-6. Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture

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