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Vol. 8(37), pp. 3422-3434, 10 September, 2014 DOI: 10.5897/AJMR2014.6802 Article Number: B5A96EE47927 ISSN 1996-0808 African Journal of Microbiology Research Copyright © 2014 Author(s) retain the copyrighht of this article http://www.academicjournals.org/AJMR Full Length Research Paper Morphological, physiological and pathogenic variability of small-spore Alternaria sp. causinng leaf blight of Solanaceous plants in Allgeria BESSADAT Nabahat1*, SIMONEAU Philippe2, BENICHOU Soumaya1, SETTI Benali3, Kihal Mabrouk1 and HENNI Djamel Eddine1 1Laboratoire de Microbiologie Appliquée, Université d’Oran Es-Senia, BP15224 El M’naouer 31000 Oran, Algeria. 2SFR QUASAV 4207, UMR 1345 IRHS, Université d’Angers, 2 boulevard Lavoisier, France. 3Institut des Sciences Agronomiques, Université de Chlef, Algeria. Received 25 February, 2014; Acceppted 9 June, 2014 Due to premature defoliation, early blight epidemics can cause major yield losses. Large-spore Alternaria species such as A. solani and A. tomatophila have long been recognized as important pathogens responsible for such blight disease in the famiily Solanaceeae and thus represent a serious risk for crop production. Small-spore Alternaria species have also been frequently isolated from plant samples with typical blight symptoms but their incidence as primary pathogens is often controversial. In order to study the diversity of small-spore Alternaria species, 32 isolates were selected from a larger collection of 130 isolates from infected leaves, fruits and sttems of tomato from various growing regions of North-West Algeria. Morphological characterization under standard conditions annd polymerase chain reaction (PCR) analyses using specific primers to amplify a part of the ITS regions and the 5.8S gene were conducted to confirm their identification as members of the altternata section.
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