Proceedings of the 2nd RP-USA Phycol ory Sympo sium/VYorkshop
Symposium: Cebu Plaza Holel, Cebu City, 6-10 January 1992 Workshop: Silliman University Marine Laboratory, Dumaguete City, i1-18 January 1992
"tfheme: Contemporary concepts and methods in systematics, biology and ecology of marine algae, including seagrasses, in the Philippines.
.NA i I I
{iponsored by: The US National Science Foundation
'SUPPORTING AGENCIES: Department of Foreign Atfairs-Office of lnternational Cooperation for Science and Technology * Department of Agricuiture-Bureau of Fisheries and Aquatic Resources * Department of [:nvironment and Natural Resources-Environmental Management Bureau * Department of Science and T.echnology-Philippine Council for Aquatic and Marine Research and Development * Office of the t'resident-Technology and Livelihood Resource Center * Seaweed lnformation Center, U.P. Marine Science lnstitute * Seaweed lndustry Association of the Philippines * Silliman University * Smithsonian l rstitution.Museum of Natural History RP COORDINATORS " Philippine Council for Aquatic and Marine Research and Development (PCAMRD) * Silliman University Marine Laboratory (SUML) * Seaweed lndustry Association of the Philippines (SIAP)
US COOROINATOR * Smithsonian lnstitution Oceanographic Sorting Center-National Museum of Natural History (SOSC-NM NH)
TECHNICAL COMMITTEE " Seaweed lntormation Center, University of the Philippines Marine Science lnstitute (SICEN, UP-MSl) * Silliman University Marine Laboratory * Department of Agriculture-Bureau of Fisheries and Aquatic Resources (BFAR)
PROGRAM COMMITTEE * Seaweed lndustry Association of the Philippines * Silliman University Marine Laboratory
FINANCE COMMITTEE * Seaweed lndustry Association of the Philippines IHicat€( * Department of Science and Technology-Philippine Council for Aquatic and Marine Research and Development (DOST-PCAMRD) .{JJ PROMOTION COMMITTEE * Office of the President-Technology and Livelihood Resource Center fl-LRC) * Department of Environment and Natural Resources-Environment Management Bureau (DENR-EMB)
SECRETARIAT * Department of Science and Technology-Philippine Council for Aquatic and Marine Research and Development * Seaweed lndustry Association of the Philippines
Editors: Hilconida P. Calumpong Ernani G. Menez
Technical Editor: Roy Olsen D de Leon
Publisher: Philippine Council for Aquatic and Marine Research and Development (PCAMRD) Los Banos, Laguna
Printer: Silliman University Press, Dumaguete City, Philippines 6200
lsBN 971-8s30-04-5 ]- Dedicated to the Philippines'two foremost phyaologists:
: Dr. Gregorio T. Velasquez .{:l.i and
Dr. Artemio V. Manza / t1 .,1 p
Ififi^r 't- I'roccedings of the 2nd RP-USA Phycolog5r Symposium,4Vorkshop
TABLE OF CONTENTS
Foreword
CHAPTER I. INTRODUCTION
Opening Remarks R.D. Guerrero IIL ...... i......
Wclcome Remarks F. Macaranqs ......
Notes on the First USA-Philippines Phycology Workshop E. Menez
Overview of the 2nd RP-USA Phycology SymposiumflVorkshop H. P. Ca\umpon7 ...... 11
Keynote Address S. Bqcani l5
A Review of Research Trends on the Marine Bcnthic Algae of the Philippines G.C.,.Trono, lr...... t9
The State of the Seaweed Industry of the Philippines B.U. Dakay 23
CHAPTER TI. SYSTEMATICS
The Marine Vegetation of Muscat Area, Sultanate of Oman P.A. Cordero, Jr...... 31
Notes on the Taxonomy of Gracilaria and Sargassum G.C. Trono. Jr...... 45 ,1
Biogeographic Affinities of Macrobenthic Algae in the Philippines M.D. Fortes 53
Molecular Characterization of Species and Populations in the Red Algal Agarophytes Gracilaria and Gracilariopsis Tablc of C.ontents
Centric Diatoms of Laguna de Bay, Philippines M.R. Martincz ...'...... 83
Phenetics and Chemosystematics as Applied to Philippine Laurencia H.P. Calumpong ...... 83
CHAPTER III. NATURAL PRODUCTS
Yield and Some Properties of Agar hom Gracilaia corornpifulia and G. eucheumoides B.T. Que ...... 87
Sodium Alginate from Philippine Sargassun: Dependence of its Viscosity and Yield on Seasonality and Pretreatment F. Sumcra, M. Pepito and E. Corpuz ...... 93
Molecular Analysis of Phycocolloid Quality from Philippine Strains of Eu cheuma and Kappaphycus H.P. Calumpong V. Vreeland, E. Solis-Duran, M. Magbanua, F. Cabanag and P. Suarez .... 109
Electrophoretic Analysis of Carrageenan Substructure V. Vreeland, E. Zablackis, H.P. Calumpong F. Cabanag E. Solis-Duran and M. Magbanua 119
Tissue Prints inRapid Screening of Carrageenan Composition V. Vreeland, M. Magbanua, E. Solis-Duran, F. Cabanag and H.P. Calumpong ...... 721,
Agar from Gracilnriopsis heterochdn from Panay Island, Philippines T.R. de Castro .. 123
CHAPTERTV. PHYSIOLOGY
Temporal Distribution and Phenology of Saryassurz Population in Maydolong Reef, Eastern Philippines H.R. Montes, Jr...... 127
Biology of Bostrychb, Stictosiplwnifl and Calogbssa (Rhodophyta, Ceramiales) J.A. West, G. Zuccarello, U. Karsten and H.P. Calumpong ...... 145 Pmceedings of the 2nd RP-USA Phycologt SymposiumAYor*shop
Euclwuma and Kappaphycus Branch and Tissue Culture at LIPMSI C. J. Dawes, G.C.,frorw,lr. and A.O. Lluisma 163 //
Studies on the Culture of Gracilaria coronopifulia J. Agardh from Carpospores W.H. Uy, M. Balanay, D. Dagapioso and M. Ologuin 169
The Reproductive Phenology of Saryassum spp. (Fucales, Phaeophyta) in Bolinao, Pangasinan G.C. TpVo,,/ Jr. and G.L. Tolentirw 181
Photosynthetic Responses of the Different Strains csf Eucheuma de nt ic ulatum and Kappap hy cus alv arezii (Rhodophyta, Solieriaceae) MA.D. de Castro and G.Cflrorw, Jr...... 195
Variability in the Estimated Minimum Number of Quadrats Yielding the Asymptote Value of Diversity in Different Seaweed Communities RN. Rollon, J.P. Tiquio and M.D. Fortes ...... 209
Growth Rates of Kappaphycus alvarezii and Euchcuma denticulatum in Occasionally Exposed and Fully Submerged Environments G.Q. Baltazar ...... 223
Photosynthetic-Irradiance Curves and Pigment Characteristics of Forms of Philippine Eucheuma Cultured in the l^aboratory C.J. Dawes and D. Kohrs ...... 225
Nuclear Genome Characterization of the Carrageenophyte Agardhiella su buhtu (Rhodophyta) D. F. Kapraum, J A. Dutcher and J. Lopez-Bautista ...... 225
The Photosynthetic Responses of the Agarophyte, Gelidizlla acerosa E.T. Ganzon-Fortes 227
Development of the Agarophyte, Gelidiella acerosa from Spores E.T. Ganzon-Forles and B. de Verccia 229
CHAPTERV. PRODUCTION
Evaluation of Seaweed Farming and its Relevance to Rural Agro-Industrial Development of Coastal Communities in the Philippines M.N. Delmendo, V.B. Alvarez and H.R. Rabanal 233 Tablc otConten*
Effects of Biological, Physical and Socio-Economic Factors on the Productivity of Eucheuma I Kappaphycus Farming Industry G.C. !.rono, Jr...... 239
Assessment of the Natural Stocks of Gelidiella acerosa (Forssk.) Feld. et Hamel in Mantigue Is., Camiquin, Southern Philippines M.D. Fortes 247
Development of Culture Techniques for Local Abalone Integrated to EucheumaFarm I.V. Manzano andV.B, Manmno 257
CHAPTER VI. DINOFLAGELI,ATES
The Culture of Three Dinoflagellate Species Associated with Ciquatera D.L. Ballantine, A.T- Bardales, M.E. Alvey 26r
The 1990 Red Tide at Banban Bay,Z,ambales, Philippines:Variations in r y\ ro d i n iu m b a hame ns e Cells R. Az a nm - Corabs and V. T. Quimpang ...... 269
CHAPTER vII. SEAGRASSES
Productivity of Seagrasses in Mactan Island and Vicinity (Cebu, Central Philippines) D. Largo 277
Perfbrmance of Seagrass Transplants in Negros Island, Central Philippines and its Implications in Mitigating Degraded Shallow Coastal Areas H.P. Calumpong RC. Philips, E.G. Mefiez, J.S. Estacion, R.O.D. de Leon and M.N.R Alava ...... 295
Evolutionary Strategies in the Allocation of Biochemical Constituents in Seagrass Blades, Short Shoots, Rhizomes and Roots C.J. Dawes 315
Nutrient Enrichment of Nearshore Marine Waters in the Florida Keys and Sarasota Bay, Florida (USA): Effects of Productivity and Biomass of Seagrasses D.A. Tomasko 3r7
FOREWORD
Almost eight years ago, the "First Philippines-USA Phymlory Workshop was held in Manila and Central Visayas, Philippines, during October 24 to November 5, 1984. The goal of the Workshop was to bring together American and Filipino phy- cologists to discuss the status of Philippine phycolory and exchange information. Sup- port for this meeting was generously provided by the US National Science Foundation, the Philippine Technologr Resource Center and Silliman University. Eleven American and 15 Filipino scientists were invited speakers. There were more than 80 officially registered participants representing 37 universities, various govern- ment agencies and private industries. Thirty five papers were presented on subjects covering taxonomy and ecolory, natural products chemistry, uses of seaweeds, aquac- ulture, seaweed processing and marketing.
The first workshop accomplishments extended beyond its primary goal. There have been frequent and increased communications, including initiation of coopera- tive projects between American and Philippine biologists. Many students, researchers and faculty from the Philippines had the opportunity to visit the USA for training, observation or to initiate/complete cooperative projects. The first workshop opened communications and effected a closer relationship between our colleagues in the aca- deme and the seaweed industry. Perhaps, the most valuable result was getting the at- tention of both government and private sectors. This increased awareness has led to substantial support for phycolory in the Philippines. At the close of the "First Philip- pines-USA Phymlory Workshop", the participants resolved to establish a Seaweed Development Program which will assist in the strengthening of research and develop- ments efforts necessary to maintain and enhance marine plant resources in the coun- try. To date, this effort has blossomed into a fuIl-pledged National Seaweed Research and Development Program in the Philippines, represented by professional biologists from the academe, government agencies and private industries.
The "Second Philippines-USA Phycolory Symposium/IVorkshop" held in Cebu and Dumaguete, Philippines on January 6-18,1992 complement the first work- shop in 1984. The meeting was mostly funded by the Division of International Pro- gram of the US National Science Foundation and sponsored by the Smithsonian Institution, Silliman University, Philippine Council for Aquatic and Marine Research and Development, Department of Foreign Affairs, Department of Agriculture, De- partment of Environment and Natural Resources, University of the Philippines, De- partment of Science and Technologr, and the Seaweed Industry Association of the Philippines. Eight USA and27 Philippine scientists were core speakers, in addition to more than 90 officially registered participants representing about 50 universities, various government agencies and private entrepreneurs. Whereas the First Philippines-USA Phycology Workshop emphasized applied research, the thcme of the Second Philippines-USA Phycolory Symposium/lVork- shop was "Contemporary concepts and methods in systematics, biolory and ecology of marine algae, including seagrasses in the Philippines". Forty five papers were pre- sented covering topics on the biolory, ecolcgy, physiolory, taxonomy, laboratory cul- tures, molecular biolory, genetics, cladistics, reproductive biolory and natural products chemistry of algae and seagrasses. Additionally, the participants also visited the rwo largest seaweed processing plants, the Shemberg Marketing Corporation and the Marine Colloids of the Philippines, lncorporated, and the seaweed farrns in Cebu and Bohol islands.
After the symposium in Cebu, 50 colleagues proceeded to Dumaguete in Ne- gros Island to participate in the workshop phase. Seven workshops were held for eight days at the Silliman University Marine I-aboratory. Each was conducted by an American scientist who was assisted by a Filipino rapporteur. There were lectures, field and latoratory sessions on Ploidy Studies, Carbohydrate Chemistry, l-aboratory Ctrlture of Algae, Culture Methods of Eucheuma denticulatum and Kappaphycus al- vorezii, Culture of dinoflagellates and Methods of Measuring Productivity in Seag- rasses. Participants visited Apo Island and Sumilon Island Marine Parks. Field work was done in several offshore sites, including visits to small seaweed farms around Dumaguete.
The "Second Philippines-USA Phycology SymposiumAMorkshop" was an im- portant and unique gathering of Filipino and American phycologists and seagrass specialists and only the second of its kind in the Philippines. It provided an opporhr- nity to expkrre and plan cooperative projects as was successfully accomplished during the 1984 workshop. It was apparent, as shoun by the enthusiasm of Filipino profes- sionals and graduate students, that the meeting had stimulated and encouraged more interest in algae and scagrasses. The workshop sessions at Silliman University Marine laboratory were most popular and well-attended. The exce]lent and systematic scheduling of lecturcs, laboratory and field work allowed most colleagues to join more than one session. The participants were exposed to primary literature and given reasonably detailed lectures on specific techniques and theories which were put into practice during the laboratory periods.
As USA coordinator for the "Second Philippines-USA Phycology Sympo- sium/Workshop", I would like to thank all the participants for their contributions and generous efforts.
E. G. Mefrez INTRODUCTION
Pmceedings of the 2nd RP.USA Phycologr Symposium/Vt/or*shop
OPEMNG REMARKS
Rafael D. Guerrero III Execrrtive Director Philippine Council forAquatic and Marine Research and Development
Secretary Bacani, Undersecretary Bengpon, Undersecretary Macaranas, Dr. Mefiez, Mr. Dakay, distinguished participants and guests,ladies and gentlemen:
As Chairman of the Organizing Committee, it is my honor and pleasure to welcome you to the 2nd RP-US Phycolory Symposium.
The holding of the symposium here in Cebu City is most timely and sigrifi- cant. Our Department of Agriculture has forecasted a bright year this 1992 for the seaweed industry which has had its share of doldrums in the past year. Cebu is not only our country's Queen City of the South with a booming economy but also the center of our dynamic seaweed industry.
The First Phycolory Symposium held in Manila in 1984 succeeded in scientific exchange in seaweed research and development opening opportunitibs for our coun- tries. It is hoped that this second meeting after more than seven years will further strengthen the collaboration and cooperation among our scientists.
This program has been made possible through the sponsorship of the United States National Science Foundation. We also wish to thank the Smithsonian Institu- tion-National Museum of Natural History the Seaweed Industry Association of the Philippines, Silliman University, the U.P. Marine Science Institute and the Depart- ments of Foregn Affairs, Agriculture and Science and Technolory for providing sup- port and assistance.
On behalf of the Organizing Committee, I wish to extend to the participants and guests our warm greetings for the New Year and high hopes for a successful meeting.
Thank you. itun Proceedings of the 2nd RP-USA Phycologr SymposiumAilorkshop 339
CULTURE OF EUCHEUMA DENTICUI-ATUM N:]D KAP PAP HY C A S ALVAREZ I I .-5 ml (-80 Clinton J.H. Dawesl ,Gavinolrono Jr.2, and Arturo Lluisma2 ]t re- lDepartment of Biolory, University of South Florida, Tampa, Florida 336720U.S-A. 2Marine d for Science Institute, University of the Philippines, Diliman, ually Quezon City, 1101 Philippines '00f ', C for Introduction .-rme- 'awa- Branch, micropropagation and callus culture has been accomplished for three forms of Eucheuma denticulatum (Brunan) collins and four forms of Kappaphycus alvarezii (Doty) Doty (Dawes and Koch, 1991). The objectives of this study are three fold: 1) To produce a standard inexpensive culture procedures that will permit the rhy'si- maintenance of seed stocks that were selected for optimal seasonal growth and leld nt to of carrageenan from farmed seaweeds (branch culture, micropropagation); 2) To ropi- produce callus tissue for propagation of a large number os selected strains so that lis! clonal material can be made available for field mariculture; 3) to demonstrate that a culture facility could supply seed stock to the seaweed farms in the Philippines. The techniques reported here were developed with funding from the U.S. Agency for In- ,lace- ternational Development and have resulted in the establishment of a pilot culture fa- ltton ciliff in the new Marine Science Institute Building on the carrrpuS of the University of Lples. the Philippines at Quezon City. pets. ; and Handling and Culture Methods.
processing. 'ith a Initial Branches up to 30 cm long are shipped up to four days h the packed detween layers of dried and rewetted Sargassum or wet paper towels. The lina branches should not touch each other and there should be no air spaces between the anes. layers. The branches are gently cleaned in filtered seawater, the epiphytes and debris re or removed, and the branches kept moist during shipping. ezing After arrival, the branches are rinsed in filtered seawater and placed in large flasks such as Fernbach flasks (28ffi ml) with 2000 ryl filtered seawater overnight at ,lace- 25oC and a 10 to 12 hour photoperiod (ca 25 p.E *-2.-'). The next day the bra-nches must are gently damp dried in clean paper towels and dipped (ca 30 sec) in a cleaning anti- : and biotic mixrr"rre (full strength in seawater, 10 min, Table 1). The branches are returned to the Fernbach flasks and placed in a modified ESS media (Saga, 1986; half strength, Table 2) and an antibiotic mixture (one Js or ml L-', Table 3). These procedures are repeated I) :ping over the next two to five days. Myo- inositol can be added (one mg L to improve would healing (in addition to that in standard ESS). After the branches el al.z of Euchcuma dcnticulatum and KappplEats alvarezii Dawes Cultun Pmceedings of thr
arc established in culture (one to two weeks) they should be pulses fed for not more of media. If ba 24 hours per week in ESS media if maintained in Bernbach flasks to avoid bac- than sulfate (stock -, terial qorltamination. Irradiance levels can vary but usually are effective from 25 to 50 -2 -l cillin G (stock pr,m s Sigma N 8878) L-t media). Th Culture procedures. A number of culture media can be used to culture the free. At that pr two species including ESS (Table 2). SWMD-1 (Chen and Taylor, 198), VS media seawater with u (Guiry and Cunningham, 1984) and Erdschreibers soil extract (Maclachlin, 1973). We selected a modified version of ESS enriched autoclaved seawater (Table 2) and Micropagation found that epiphyte contamination was lowest if enriched seawater was alternated with sterile seawater on a weekly basis Myo-inositol appears to be important in A rapid wound hcaling of the cut branches (Azanza-Cnnales and Dawes, 1989). AII stock so- that are 0.5 cm lutions are stored at 10oC or lower. Seawater is autoclaved nvice (30 min, 121oC) and wells) have bee all glassware (l x20 min, 121oC). Media is made up as needed in Fernbach flask us- soaked in distill ing autoclaved pipettor tips because of precipitation or contamination if larger wave oven (full amounts of media are left standing at room temperature. To start Saliniry of the culture media should be 34 to 36 ppt because both species are riched seawater stenohaline. However, during cleaning brief (30 sec) rinses in tap water will not nar flow hood, damage the plants. Philippine species grow best in temperatur.es of 22 to 26oC while explants are so higher (30oq temperatures usually result in bacterial contamination and require mixture (Table more frequent changes of media. Temperature-s lower than 20oC should be avoided seawater enrict
(Dawes, feA9l. t..aAiance levels of 50 pE rn-2r-1 u." sufficient for normal culture and (PGRs) given i growth rates irave been higher (one to three per cent day-l) in irradiances of 100 to (NAA) or indol 160pE m-"s-' if branches are acclimated. At least one half of the flourescent bulbs tenyll adenine) should be "plant grow" types and the photoperiod should be about 12h D and 12h L. stored frozen. I The pH of the media should be 7.5 to 8.0. Aeration was not found to be effective. Table 3) can br and lZ:l2lightlr Branch Culture During t Clean segments, five to nine cm in length are transferred into crystallizing lary area (cut a (200 (Table Plant-s dishes or equivalent to 300 ml) with the modified ESS media 2). plants should b, are rinsed and brushed under tap water for about one minute. If contamination is 2iP or kinetin). visible, the branches can be cleaned with a tooth brush to remove the epiphytes and ml) multiwell tr ice-ice white spots and dipped for 30 sec cleaning antibiotic mixture (Table 1, full out addition of weeks. strength) every two to three A dilute (5 pg L-') solution of GeOZ can be soon as the pla added to the culture media to inhibit diatom development. The addition should not micropropagule pg be used longer than 2 weeks or should the concentration be above 5 as the sea- dish. At that po weeds are sensitive to the compound. Callus Producti Antibiotics can be used both for initial cleaning (full strength, Table 1 or 3) and during continuous culturing adding one milligram of stock (Table 3) to each liter Callus w two types: cryst Proceedings of the 2nd RP-USA Phycologt Symposium/Worlshop 341
of media. If bacterial or blue-green algal contamination becoms visible, polymixin B sulfate (stock -38.5 mg/L - 300 units/L; Sigma P-1m4, 7800 unitslg) is effective. Peni- cillin G (stock - 75 mglL; Sigma 7794) and Naldixic Acid (stock - 75 mg/200 ml; Sigma N 8878) are also useful alternatives during continuous culiure (add 1 ml stock L-t media). The cleaning procedure is repeated weekly until the branches are algae- free. At that point, the media can be changed alternating a week with ESS enriched seawater with up to two or three weeks of sterile seawater.
Micropagation
A rapid (2 month) method for clonal propagation of branches utilizes explants that are 0.5 cm long. Falcon Multiwell Tissue Culture Plates (24 x 3 and 6 x 10 ml wells) have been used. These plates can be sterilized in 10 per cent bleach overnight, soaked in distilled water for 24 hours and after thorough dry,ng, sterilized in a micro- wave oven (full strength, four by four minute exposure).
To start the micropropagation procedure, epiphyte-free branches from en- riched seawater culture care cut into 0.5 cm explants under sterile conditions (lami- nar flow hood, flamed tweezers and scalpels, sterilized glassware and liquids). The explants are soaked for 30 minutes to one hour in full antibiotic strength cleaning mixture (Table 1). Each explant is placed in a 3 ml multiwell with 2 ml of sterilized seawater enriched with modified ESSs without the standard plant growth regulators 1 (PGRs) given in Table 2, and 10 mg L of the auxin alpha napthalenacetic acid iNeel oi indol-3-brtlric acid (IBA) and 1 mg L-l of the cytokinini Zip gt' lisopen- tenyl] adenine) or kinetin (Stock- | mglL).The stock solutions of all PGR's can be stored frozen. I-ower levels of the appropriate antibiotic (1 ml of stock L-l of media; Table 3) can be added. Multiwell plates should be incubated at 25oC, 25 p.E m'ts-l and 12:12 light/dark photoperiod.
During the first week, one to two millimeter growth is observed at the medul- lary area (cut area) as well as elongation of the spines. After the four weels, the ex- plants should be transfered into new multiwells with media (ESS + NAA or IBA + 2iP or kinetin). After six to eight weeks, the explants can be transferred to larger (10 ml) multiwell trays or crystallizing dishes with ESS enriched seawater (Table 2) with- out addition of rytokinins. The auxins can be eliminated from the culture media as soon as the plant reaches one to two cm in size. After four months in culture, the micropropagules will reach three to four grams (wet weight) filling up a 300 ml petri dish. At that point, these plants can be used to generate new explants.
Callus Production
Callus will develop at the cut ends of explants of both species. The callus is of two types: crystalline hairs which will not regenerate into new plants and brown to and Kappaplryan alvarezit Dawes e/ aJ.: Cultun o! Eucheuma dentiatlafiua Proceedings ofth,
tissue that eioes not regenerate. Plants with active growth and white undifferentiated Literature Cite no evident contamination are ideal as explants, thus micropropagules are a good source. Azanza-Conal, alvarezii The branches are cut into 0.4 to 0.5 cm sections under sterile conditions and (Table transferred to an antibiotic solution 1) or 300 units 100 m1-1 of polymixin B. Chen, L.C. and prepare
Dawes, C.J. 19I Callus production has been succesful {50 to 757o of explants) in three to eigh: Florida: per ESS enriched (Table 2) autoclaved seawatc: cent agar {very hard) containing 59_65. with the addition of FGRs. The agar mixture is viscous and the test tubes or platc: must be poured while hot. These tubes and plates are then autoclaved and allowed tt' Dawes, C.J. and cool on a slant. A concentration oi' tr rng tr--' each of ihe auxin IBA and either cytok- red nin BAP or 2iP is recommended for callus production although different forms c: algae Philippir each spccies responded to other PGRs as well.
Guiry, M.D. The axenic explants are transfered to the tissue culture media, and insertc; anr response into the agar so that one cut end of the cxplant is cxposed to the air, which appears l. (Rhodop be crucial for rallus psroduction. Within the first two weeks, fuzry crystalline hairs r'. an amorphous brown to white to pink callus develops on the cut end. Develciprncl': , . the callus may take two to 10 weeks, depending on the fbrm. Mcl-achlan, J. 1l of Phycol Regeneratloi'n Cambridl
The callus is e,. Conclusioms The culturc techniques devcloped tor Eucheumo denticulatum and Kappaph. cus alvarezii will allow thc production of sccd stock for the se aweed farms in the Ph ippincs. Cost analysis and the modification of culturc mcthods are now being tcst--: to rcduce the expense of seaweed culture in the laboratory. A cuiture faciliry is bci:_- designcd to function in ihc seawc,:d tarming regions to test uscfulness of the cultu: proccciure . Proceedings of the 2nd RP-USA Phycologt SymposiumAilorkshop 343 Literature Cited Azanza-Corrales, R. and C.J. Dawes. 1989. Wound healing in cultured Euchcuma alv arezi i v ar. ta mbal a ng D oly. Bot. Mar. 32:229 -234. Chen, L.C. and R.A. Taylor. 1978. Medullary tissue culture of the red alga Chondrus crispus. Can. J. Bot.,56:883-886. Dawes, C.J. 1989. Temperature acclimation of cultured Eucheuma bifurme from Florida and Eucheuma alvarezii from the Philippines. J. Applied Phycol. 59-65. Dawes, C.J. and E.W. Koch. 1991. Branch, micropropagule and tissue culture of the red algae Eucheums denticulatum and Kappaphycus alvarezii farmed in the Philippines. J. Appiied Phycol. 3. Guiry, M.D. anmmd E.M. Cunningham. 1984. Photoperiodic and temperature responses in the reproduction of north-eastern Atlantic Gigartinn acicularis ( Rhodophyta : Gigartinales). Phycol o gia 23 : 3 57 -367 . Mclachlan, J.1973. Growth media marine. pp25-51.In: J.R. Stein (ed.), Handbook of Phycological Methods. Culture Methods and Growth Measurements. Cambridge, Univ. Press, London. Saga N. 1986. Pure culture of algae (in Japanese). pp 55. In: Y. Yamada and Y. Okada (eds.), Plant Biotechnolog5z., Tokyo Kaymkes Dojin, Tokyo. 34 Dawes et aL: Cultun of Eucluunu dcntiaiatum and lhppptryats alvaraii Proceedings of tht Table l. Cleaning antibiotic mixture to be used full strength for cleaning of fresh Table 3. Standr branches with short (30 sec to 30 min) periods. It can be added to either (add ESS or SWMD-I culture media (add 1 ml of stock solution to each liter of also I culture media) for short periods (one week). 36 mgA Polymixin B Sulfate 15 mgA Erythromycin 30 mg/l Neomycin 10 mg/l Gentamicin 30 mgn Ripfamin 300 sl Naldixic acid Table 2. Ess-l seawater enrichment modified from saga (1986). one milligram of each stock is added to a liter ofsterilized seawater. Metals and Salts Stock NaO: 15.0g Na glycerophosphate 2.0s(26 pMP) Fe sequestrene (EDTA) 1.og KI 0.00259 ZnAz 0.$4Alg t I"CP .4HzO 0.04948g CoCI'.6 HzO 0.0592169 FeSoa .7 Hzo 0.069489 EDTA .zLIzO 0.09305g HaBO: 0.015469 Vitamins and Minerals Stock Vitamins Brz 0.002509 Biotin 0.002509 Thiamine HCI 0.250009 Nicotinic acid 0.250009 D-panthotenic acid 0.250009 p-aminobenzonic acid (hemicalciuur salt) 0.025009 Myo-inositol 2.500009 Thymine 0.250009 Auxin Stock Alpha-NAA or tBA 0.002509 Qtokinin Stock 2iP or kinetin 0.002509 Proceedings of the 2nd RI'-U SA Phycology Symposiunr,AVorks hop 'l'able 3. Standard antibiotic mixture to be added to ESS or SWMD-l culture media (add I ml of stock solution to each liter of culture media). l'his mixture can also be used full strength as a rinse (30 sec to 30 min). 0.3 g/lPenicillin G 0.1 g/lStreptomycin 0.05 gl Neomycin 0.2 g/l Kanamycin I I Proceedings ofthr Grego New York Gty Botany at the U He war his elementary college at the t tant in Botany ir was appointed a 1931, he finishe Procecdings of the 2nd RP-USA Phycologi SymposiumlWorkshop 347 Abelated IN MEMORIAM Gregorio Tiongson Velasquez 2 September l90l - 29 July 1989 Gregorio T. Velasquez died of heart attack at the Montefiore Hospital ln New York City, on 29 July 1989, at the age of 87. He was Professor Emeritus 1n Botany at the University of the Philippines where he had worked since 1923. He was born on 2 September, 1901 in Calumpit, Bulacan, Phiiippines. After his elementary and secondary schooling in his hometown, Dr. Velasquez attended college at the University of the Philippines where he started as a l-aboratory Assis- tant in Botany in 1923" Upon completion of his Bachelor of Science in 1,925,he was appointed as Assistant Instructor. By 1926, he rose to full Botany Instructor. In 1931, he finished his Master of Science and stayed until 1936 when he left on a fel- 3.t8 MEMORIAM: Grcprio Tnngon Yelaqrzz Proceedings of the lowship to pursue advanced studies in the Botany Department of the University of 1969 Certifice Michigan in Ann Arbor. There, he completed a Master of Arts in 1937 and a Ph.D. Emeritu in 1939. Dr. Velasquez returned to the Philippines with his wife and a daughter and joined the faculty of the Department of Botany, University of the Philippines as As- 1970 Biologisr sistant Professor and Head of the department in 1945 to 1955. He was the Founder (BrorA and President of the Phycological Society of the Philippines, Incorporated from 1955 until his death in 1989. Dr. Velasquez was Founder and Adviser of the Alpha Chi 1972 Cultural Chapter, Phi Sigma Biological Honor Society at the University of the Philippines and its Permanent Council Representative since 1950. The Society made him an Honor- ary Member in 1968 and honored him as their Most Distinguished Member in 1V76. Award fc The Grel Five In 1958, he became Fuli Professor and upon his retirement in 1967 was ac- mol corded the rank of Professor Emeritus in Botany. For his many contributions to sci- Biologiu ence and for pioneering in Philippine phycolory, he received h 1982 the most prestigious Presidential Award in Science, the rank and title of "National Scientist". 1914 Apprecia Dr. Velasquez' thirly years of productive work, as researcher and educator. 1975 Most Ou has earned him many other awards and honors which include the following: Homecor Philippin 1950 Outstanding Educator, Philippines Herald's "Who's Who in Education". Most Out 1956 Scientist of the Year and recipient of the Distinguished Science Medal and Celebrati Diploma of Honor, Republic of the Philippines 1976 Outstand John Simon Guggenheim Memorial Foundation Fellowship Associati 1965 Certificate of Appreciation, University of the Philippines Natural Science 1977 Plaqueol Research Council in recognition of his contributions and unselfish devotion Philippin to science and higher education. . growth al Recrgniti 1967 Certificate of Appreciation, awarded by the Alpha Chi Chapter, Phi Sigma "978 Biological Honor Society for his continuous services to the Chapter as scientific Founder, University of the Philippines. 1978 Philippin Teacher, Scientist and Researcher award, Department of Botany, Universiq recogniti< of the Philippines. 7th Gera 1968 Member Emeritus, New York Academy of Sciences, USA.. biological -982 Outstand l+r: Proceediogs of the 2nd RP-USA Phycologr SymposiumAilorkshop 3.19 Sn of i969 Certificate of Merit for distinguished service as a phycologist and Professor Ph.D. Emeritus of Botany, Dictionary of International Biography, Vol. VI, l-ondon. :r and rs As- 7970 Biologist of the Year award, Biolory Teachers Association of the Philippines under (BrorA). r 1955 ra Chi l97Z Cultural Heritage Award in Science, Republic of the Philippines. :s and lonor- for to the National Botanic Garden activities. r976. Award dedication The Gregorio T. Velasquez lrcture Series, held in his honor in1972-7973. Five monthly lectures sponsored by the Alpha Chi Chapter of the Phi Sigma as ac- Biological Honor Society. to sci- most ist". 1974 Appreciation Award, as Director of the Philippines ScienceHigh School. rcator, lg75 Most Outstanding Alumnus,in the Natural Sciences, awarded during the First Homecoming Reunion of the College of Arts and Sciences, University of the Philippines. Most Outstanding Scientist, Calumpit, Bulacan Fourth Centennial Celebration. nd 1,976 Outstanding Scientist award, 25th Foundation Anniversary of the Philippine Association for the Advancement of Science. 1977 Plaque of Merit, awarded by the National Research Council of the for his unselfish and dedicated service, 1966 to 1973, to the ton Philippines, . growth and development of biological research in the country. 1,978 Recognition Award, from the Phycological Society of the Philippines for his scientific accomplishments and dedicated efforts as educator and researcher. 1978 Philippine National Academy of Science and Technologi, Member, in recognition of his distinguished achievements in the field of Botany. sity 7th Gerardo O. Ocfemia Memorial Award, for his contributions to biological research in the Philippines. lg82 Outstanding Bulakefro Award in the field of science and invention. 350 MEMORIAM: Cregotio T'iongan Yela.qrcz Proceedings oft Outstanding Scientist Award, Philippine Association for the Advancement of 1939. On rhe Science. (Doros 2z(2):. 1984 Service Award, 1965-1973 and 1975-1983, National Research Council of the Philippines. L940a. A list r 1985 Order of the Oblation, Dean's List, University of thePhilippines. 1940b. Filame Science Plaque of Appreciation for his meritorious services and outstanding contributions to initiate the founding of the Micro-biological laboratory for 1941a. Filame service to science and education. 1941b. Filame 1986 Plaque of Appreciation, presented by the National Committee on Marine Sciences, Quezon Gty. 1948. Survey r Americi 1987 Member Emeritus Award, National Research Council of the Philippines,54th Annual Meeting, Metro Manila. 1950. Studies Applied After his retirement in 1967, he continued teaching at the University of the Philippines as Professorial kcturer without compensation and also taught at the 1951a. (M. Can (Manila graduate Pamantasan ng Lungsod ng Maynila City School) and the school properti of Centro Escolar University. In 1974-195, he accepted the position of SEATO Pharma< (Southeast Asia Treaty Organization) Professor at Kasetsart University in Bangkok Thailand. From 1959 to 1987, he held the position of Contributing Editor of the Phil l9s1b. ippineJournalof Scienceand Chairman of the Biological Sciences Division,Phii- (G.T. V ippine National Science Society. Dr. Velasquez' name is listed in the Philippin: seaweed: Men of Science, American Men of Science, World's Who's Who in Science and the Dictionary of International Biography in Science. He belonged to about 30local anc 19-51c. (G.T. Vr international organizations and societies, and has represented the Philippines ir-. vicinity. many international meetings and conferences. Even after his retirement, Dr. Ve- lasquez continued to conduct research on the blue-green algae resulting in more tha;: 1951d. Additior 20 publications. Builetin , Dr. Gregorio T. Velasquez, the educator and scientist, had more than 50 br- ,952a. (G.T.VeJ tanical articles and books published, including 29 other articles on various topics coll- vicinity. ) cerning science which he presented as invited speaker or lecturer. - 9-52b. 1lr..rnu1 G.T. Velasquez' publications: !,i2c. Algalpol 1931. Anatomical study of the culm of five Philippine bamboos. Natural and rbid. 12(3 Applied Science Bulletin 1(4): 281-318. Proceedings of the 2nd RP-USA Phycologr SymposiumfiYorlchop 351 1939. On the viability of algae obtained from the digestive tract of giuard shad (Dorosoma cepedianum) (ksueur, 1818). American Midland Naturalist 22(2):376-412. 1940a. A list of filamentous Myxophyceae from Michigan. Ibid.23(1): 178-181. 1940b. Filamentous Myxoptiyceae of the Philippines. 1. Natural and Applied Science Bulletin 7 (3): 269 -27 1. l94la. Filamentous Mlxophyceae of the Philippines. II. Ibid. 8(2): 189-200. 194tb. Filamentous Myxophyceae of the Philippines. III. Ibid. 8(2):203-210. 1948. Survey of the algae and economic algal resources (Progress Report). American Philosophical Society Yearbook, pp. 154-155. Y o 1950. Studies on the Mlxophyceae of Puerto Galera and vicinity. Natural and Applied Science Bulletin 10(4): 187-19a. 1951a. (M. Cantoria, P. Valenzuela and G.T. Velasquez) Pharmacopoeial properties of agar from three Philippine seaweeds. Journal of the Philippine Pharmaceutical Association 38(7): 187-194. I951b. (G.T. Velasquez and M. Cantoria) A botanical study of rhree Philippine seaweeds. Ibid. 38(10): 295-304, 9 figs. 1951c. (G.T. Velasquez and J. D. Soriano) Sub-aerial Myxophyceae of Manila and vicinity. Ibid. 38(12): 372-380. 1951d. Additional record of Philippine Myxophlceae. Natural and Applied Science Builetin 11(3 & 4):208-229. 1952a. (G.T.velasquez andJ.D.Soriano) Studies on the Myxophyceae of Manila and vicinity. Ibid. 12(1): Z-92,1map, 4 pls. and 36 figs. 1 952b. Thermal algae of the Philippines. Ibid. 12(2): 137 -1,47 . 1952c. Algal poliution from ponds at Puerto Galera, oriental Mindoro, philippines. Ibid. 12(3): 239-257, 3 pls. 352 MEMORIAM: Grcgorio Twrymn Vehqwz Proceedings ofth 1953a. Studies on the marine algae of the Philippines. In: Proceedings of the 1962. How al Eight Pacific Science Congress (November 16-18, 1953, Quezon City, 32-34. Philippines). pp. ?n5 -206. 1963. (c.T. V 1953b. Seaweed resources of the Philippines. In: Proceedings of the First occurre International Seaweed Symposium (July 14-17, 1952, Edinburgh, Scotland). 483-522 pp. 100-101. 1966a- Prepara 1955a. The ecological distribution of the Myxophyceae in Eastern Palawan and Sulu Philippi Province. Natural and Applied Science Bulletin 1,5Q-\:155-184,5 pls. 1966b. Biolog 1955b. Anomalous growth in coconut palms from the Philippines. Turtox News 33(5): 10G101. 1967a. Science 1956. The marine biological station of the University of the Philippines. tbid. 3a(f : 1967b. Seawee 2,2figs. 1968a. Ten edi 1957a. Onthestate of phycblogical knowledge in the Philippines. In: Proceedings of tt8-122 the Ninth Pacific Science Congress (November 18-December 9,195'1, Bangkok, Thailand). pp.2i4?38. 1968b. A biolo The Bio 1957b. Further study of the thermal algae of the Philippines. In: Proceedings of the Eight Pacific Science Congress (November 16-?3,1953, City, Quezon 1969. Seaweec Philippines). pp. 455 -457. Proceed sources. 1957c. (G.T. Velasquez and J. D. Soriano) The ecolory of the Philippine Phiiippir Myxophyceae. Ibid. pp. 483-490. i97C. (G.T. Ve 1957d. Studies on the marine algae of the Philippines. Philippine Journal of Science and prot 9l(3):267-380, 13 pls., 149 figs. Science ! 1957e. Alpha Chi Chapter in Retrospeet. The Biologist 39(1-a):59. 197La. (G.T. Vr commun 1959a. (G.T. Velasquez and J.IC Santos) Practical Botany, A laboratory Manual. Bataan. Revised Edition. Macaraig PublishingCompany, [ncorporated, Manila, 144 pages. 1971b" Studies r Seventh 1959b. The re-evaluation of teaching biological sciences in the University of the Japan). p Philippines. The Biologist XLII(1-2): 5-7. Proceedings of the 2nd RP-USA Phycologr Symposium/Worlshop 353 1962. How a Iocal biological station should be organized. The Biologist 46(1-2): 32-34. 1963. (G.T. Velasquez and E.C. Galutira) Taxonomy, distribution and seasonal occurence of edible marine algae in llocos Norte, Philippines. Ibid' 92(a): 483-522, 9 pls.,37 figs. 1966a. Preparation of algae exsiccatae for the Philippines. University of the Philippines Research Digest Y (2): 52-5 4. 1966b. Biolory today. The Philippine Biota 1(2):53-56. i967a. Science and public policy. Science Review 8(7): 31-33. I 967b. Seaweed resources and conselvation. Ibid. 8(10): 19-22. w - 968a. Ten edible seaweeds of the Philippines. The Philippine BIOTA 2(3): he 118-122,10 figs. - 168b. A biological approach to the national food sufficiency of the Philippines. The Biologist L(3-a): U2-147. +,9. Seaweed utilization and sugg;estion for development in the Philippines. In: Proceedings of the Seminar in Production of protein-rich foods from local sources. (January 23-25,1969, Centro Escolar University, Maniia. Philippines). pp. 120-130. -- , {G.T. Velasquez and D.F. Cornejo) Study on the algai epiphytes or exnosei and protected marine water.i of Batangas Frovince. Fhilippine Joui'r:?l i:i Science 99(3-4):165-190, 1 nrap, 5 pll.,40 rige. ,r. (C.T" Veiasquez, D.F. Comejo, A.F.. Santiago and 1..8. Arcega) Ngai communities of exposed anri proiected rnarine waters r:f Batangas ani Bataan. lbid. 1C0(1): l-4,J.,11pls., 69 photosrapn" -,. Studies and utilization of th; Fhilipprne marine aigae. II: Froceedings of the Seventh lnternational Seaweed Symposium iAugust 8-12, 19?1, Sappor'"r, r.i Japan). pp. 62-65, 2 tigs. e.Ce ,.4^ 354 MEMORIAI\4' Gregofio Twngrcn Velaqwz Proceedings ofth 1971c. Taxonomic study and ecology of the useful marine algae of the Philippines. 1985b. The tec In: Proceedings of the Twelfth Pacific Science Congress (August Philipp 1S-September 3,1971, Canberra, Australia). pp. 89-90. Techno l97ld. Some Philippine marine algae. [n: Plants of the Philippines. University of 1987. Abriefl the Philippines Press. pp.419-455,39 figs. Agricul 1972. (G.T. Velasquez, G.C. Trono and M.S. Doty) AJgal species reported from the Dr. Vel Philippines. Philippine Journal of Science 101(3-a): 115-169. parasitoiogist i University of tl N 1975a. Seeing quadruple. Turtox News 52: 2. Emperatriz, 1975b. (G.T. Velasquez and Khanjanapaj kwmanomont) A checklist on the study On Au1 with milita of the benthic marine algae of Thailand. Kasetsart University Fishery full cials of the Dr Research Bulletin, No. 8. Science and T Bonifacio in M 1977. T\e blue-green algae of Kasetsart University campus and vicinity. Ibid. No. 9. Dr. Gr 19'79a. The microscopic algae of the hard coral communities. Ibid. 108(3-4): larly to me. H 121-135,4 figs. scientist and et of his fellowme 1979b. Seaweed utilization in the Philippines. Reprinted from Marine Production Mechanisms, edited by M.J. Dunbar. International Biological Programme 20: 229-232. 1979c. Food habitat in the deep. Dilliman Review, pp. 13-16. 1981. A second look at the lowly algae. The Dilliman Review 29(\: a6-a7. 1983. Blue-green algae in the Philippines. Natural and Applied Science Bulletin 35(3):207-210. 1984. Collection and taxonomic studies of the blue-green algae and natural pollution. Transactions of the National Academy of Science and Technology, Vol. VI, pp.53-60. 1985a. History of phycology in the Philippines, Part I. Review Article. Philippine Journal of Science 11a(3-a): 247-250. By: Ernani G. I Institution, Wash Silliman Universi Proceedings of the 2nd RP-USA Phycologr Symposium/YVor*shop 355 1985b. The technology of research in the blue-green algae and relevance to Philippine economy. Transactions of the National Academy of Science and Technology. Vol. VII, pp" 119-721. 1987. A brief history of the study of Philippine blue-green algae. Philippine Agriculfure 69(aB): 545-546. Dr. Velasquez is survived by his wife, Dr. Carmen Camacho-Velasquez, a rarasitologist and also a National Scientist and Professor Emeritus in Zoology at the University of the Philippines, and three daughters, Julieta (Mrs. David Butler), Maria Emperatriz, Maria Milagros (Mrs. Donald Reid) and several grandchildren. On August 8, 1989, Dr. Gregorio T. Veiasquez was accorded a state funeral * ith full military honors by the Philippine government. His family was joined by offi- .-ials of the Department of Science and Technoiogy and the National Academy of iience and Technology during the funeral services at the Heroes Cemetery at Fort Bonifacio in Manila, Philippine. w \e Dr. Gregorio Velasquez v/as a great inspiration to all his students, particu- .arly to me. He was my mentor and close friend until his death in 1989. This great .cientist and educator, and all his contributions to science and his deeds for the good 'f his fellowmen shall be remembered for all eternity. :- Ernani G. Menez, Curator, Department of Botany, Museum of Natural History, Smithsonian -rirution, Washington, D.C. 20560; Hilconida P. Calumpong, Director, SU Marine Laboratory, i .:iman University, Dumaguete City 6200, Philippines. Proceedings oftt Artem was Professor c sity of the Philitr He wa In his early year was poor and cr spent his underl Philippines in L paid for his sch and was appoinr Proceedings of the 2nd RP-USA Phycologr SymposiumAtrorkshop 357 l w he Abelated IN MEMORIAM ARTEMIO VALDERRAMA MANZA 20 October 1896 - 17 March 1964 Artemio V. Manza died of cancer, on 17 March 1964, at the age of 68. He u,as Professor of Botany and Dendrology at the College of Forestry of the Univer- sity of the Philippines in Los Baflos, laguna, where he had worked since 1939. He was born on 20 October 1896, in Lopez, Quezon Province, Philippines. In his early years in school, he wanted to pursue a medical profession but his family \r'as poor and could not afford to send him to a medical school. Instead, in 1915 he ipent his undergraduate years at the College of Agriculture of the University of the Philippines in Los Baflos as a student assistant in Mathematics and Agronomy which :aid for his schooling. He received his Bachelor of Science in Agriculture in l9?i '!he ,nd was as Instructor year. !F!Ce appointed Assistant in Botany the same He held this po- ra 358 MEMORIAM: Atlemio VaWenama Manza Proceediogs of sition until 1927 when he left for the USA to enroll at the University of California in Xi, Phi Sigm Berkeley. While there, he did various part-time jobs, e.g., lawnmower operator, poul- Fraterniry-. try keeper, dishwasher, jobs to pay school. Later, he was able to find work as a mi- crotechnologist under the late Dr. Wiliiam Albert Setchell, foremost American Dr. V phycologist. Under the latter's guidance and the assistance of Dr. A. Weber van- gae of the N Bosse, a renowned European phycologist, in 1937, Manza mmpleted his Ph.D. and five of them. dissertation entitled, "A revision of the genera of articulated corallines". After gradu- as new. Pre ation and upon Dr. Setchell's advice, Dr. Manza accepted the offer as Professor of thron was plz Botany at Lingnan University in Canton, China for about 10 months. (1940) on ar publications, Dr. Manza barely missed the Sino-Japanese war when he left Canton in Au- gust 1938, to assume his new position as Assistant Professor of Botany at the newly Dr. Manza's established branch of the University of the Philippines in Baguio City and to get mar- ried to Asuncion Anacion, his sweetheart since the time he left for the USA. His re- 1937a. The g sponsibilities as a teacher and administrator in the university kept him busy and left of Scit no time for his study of the articulated coralline algae. In his letters to Dr. Setchell, he bemoaned about his later situation in the university. 1937b. Some Natior At the time he was in Baguio City, it was the height of the Second World War and Manila, then capital of the Philippines and suburbs were frequently being bom- 1937c. New s Japanese military forces. Japanese Imperial Army invaded barded by the When the 239-31 the Philippines in 1942, they marched in and orcupied strategic and major areas of the country including Baguio City. It was then that the Manza family decided to Dr. Manzats t move to Quezon Province. In 1943, Dr. Manza accepted a position at the Bureau Science. He left the bureau during the American Liberation period in 1945. of Stu( Mount Makili ln 1946, Harold Cuzner, Forester-in-Charge at the Collegeof Forestry of the University of the Philippines in l-os Banos hired him as Assistant Professor in Bot- Grass service earned Full-Professorship in any. His distinguished at the university him a family Laurac 1955. Dr Artemio V. Manza was Founder of the ZetaBeta Rho Honor Fraternity the Philippines. his retirement October 2n,1962 he was at the University of Upon on Grass: honored by his brothers a Plaque Recognition his meritorious perform- with of for cies of the fa service profession of Forestry and for being ance and distinguished to the the Frater- characters anr 13 years. 1963, nity's guiding light for In the University of the Philippines Board campus and vi of Regents confirmed him as Professor Emeritus in Botany. During the next year, mechanical Dr. Manza died. Victor Jose A. Manza is an only child, a engineer, of We art Dr. and Mrs. Manza. Jose Vera San Dr. Manza's committee and other extra-curricular work include memberships in the University Council Committee on Student Progress, College Committee on In- vestigation, Committee on Dormitories, and Adviser for Junior, Sophomore and Jun- ior Classes. He was also a member of scientific and learned organizations as Sigma By: Ernani G. . Institution, Wasl Silliman Universi Proceedings of the 2nd RP-USA Phycologr SymposiumAVor*shop l Xi, Phi Sigma, Society for the Advancement of Research andZ,eta Beta Rho Honor Fraternity. Dr. Manza was known for his taxonomic works on the articulated coralline al- gae of the Northeastern Pacific. In his first paper (1973a), he reported 12 genera, five of them, Bossea, foculator, Calliarthron, Duthiea and Pachyarthron, he recorded as new. Presently, the new name for Bossea is Bossiclla (Silva, 1957) and Pachyar- thron was placed as a subgenus of Bossiella (Johansen, 1969). In his last publication (1940) on articulated corallines he summed up the 22tw Dr. Manza's publications: 1937 a. The genera of articulated corallines. Proceedings of the National Academy of Sciences, USA23(\: aa-a8. 1937b. Some North Pacific species of articulated corallines. Proceedings of the w National Academy of Sciences, USA 23(11): 561-567. he 1937c. New species of articulated corallines. Philippine Journal of Science, 71(3): 239-316,20 pls. Dr. Manza's unpublished works: Study of the distinctive characters of the different species of Moraceae in Mount Makiling. Grass morphology and the taxonomic importance of the timber species of the family Lauraceae inMount Makiling. Grass morphology and the taxonomic importance of leaves of the timber spe- cies of the family Dipterocarpaceae in Mount Makiling. Study of the vegetative characters and the taxonomic importance of the species of bamboos on the Forestry campus and vicinity. We are grateful to Prbf. O. Valderrama, Dr. Benito S. Tan and the late Dr. Jose Vera Santos, University of the Philippines, for their contribution and assistance. ri ct Bv: Ernani G. Mefrez, Curator, Department of Botany, Museum of Natural History, Smithsonian :he Institution, Washington, D.C. 20560; Hilconida P. Calumpong, Director, SU Marine Laboratory, Silliman University, Dumaguete City 6200, Philippines. ,.4- Proceedings oftl Renoir A. Abr Mindanao Sta Naawan, Misa Jalil Ahqia Philagar Corp, Paknaan, Man Jolly Ahqia Philagar Corpr Paknaan, Man Moonyeen Nid Marine labor: Silliman Unive Dumaguete Ci Vicente B. Alva FMC Internati 2nd Floor V & 8 Gorordo Ave Erick I. Ask c/o FMC Marir Philippines, P.O. Box 126 Mandaue Cent Mandaue City, Mariefe S. Bala Mindanao Statt \aawan, Misan David Ballantir Department of University of Pr Mayaguez, Puer