Efficient Professional and Antibody Platforms INCENP Antibody Basic information: Catalog No.: UMA60519 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: Protein A affinity purified Useful Information: WB:1:500-1:1000 ICC:1:100-1:500 Applications: IHC:1:50-1:200 FC:1:100-1:200 Reactivity: Human Specificity: This antibody recognizes INCENP protein. Immunogen: Recombinant protein A replicated includes two kinetochores that control chromo- some segregation during . The CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the and are involved in the formation of the centromere and the organization and func- Description: tion of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for . During mitosis it is also required for onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase and during anaphase, to the equatorial cortex. Uniprot: Q9NQS7(Human) BiowMW: 105 kDa Buffer: 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of INCENP on human IN- CENP recombinant protein using anti-INCENP an- tibody at 1/1,000 dilution.

Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 di- lution.

ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraf- fin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with he- matoxylin.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]