Catalog # Aliquot Size
R298-31H-20 20 µg R298-31H-50 50 µg
RanGAP1 Protein Recombinant human protein expressed in E. coli cells
Catalog # R298-31H
Lot # D2422-6
Product Description Purity
Recombinant human RanGAP1 (418-end) was expressed in E. coli cells using an N-terminal his tag. The gene accession number is NM_002883.
The purity of RanGAP1 protein was Gene Aliases determined to be ~90% by densitometry. Fug1; RANGAP; SD Approx. MW 20kDa.
Formulation
Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, and 25% glycerol.
Storage and Stability Upstream Active Enzymes
Store product at –70oC. For optimal storage, aliquot UBE2I, Active Cat # U224-380H target into smaller quantities after centrifugation and SAE1/UBA2 complex, Active Cat # U208-380H store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background
RANGAP1, Ran GTPase activating protein 1, is the control substrate, which produces a single SUMO modification during SUMOylation (1). As a GTPase activator, RANGAP1 is involved in nuclear envelope assembly and nuclear transport through interacting with Ras-related nuclear protein 1(RAN) and regulating GTP-binding and exchange (2). Also, abnormal localization of RanGAP1 was found in cortex of the patients with Huntington’s RanGAP1 Protein disease (3). Recombinant human protein expressed in E. coli cells
Catalog # References R298-31H Lot # D2422-6 1. Joseph J., et.al. SUMO-1 targets RanGAP1 to kinetochores Purity ~90% and mitotic spindles. J. Cell Biol. 156:595-602, 2002. Concentration 0.5 µg/µl 2. Swaminathan S., et.al. RanGAP1*SUMO1 is phosphorylated Stability 1yr at –70oC from date of shipment o at the onset of mitosis and remains associated with RanBP2 Storage & Shipping Store product at –70 C. For optimal storage, aliquot target into smaller upon NPC disassembly. J. Cell Biol. 164:965-971, 2004. quantities after centrifugation and store 3. Gasset-Rosa F., et.al. Polyglutamine-Expanded Huntingtin at recommended temperature. For most Exacerbates Age-Related Disruption of Nuclear Integrity favorable performance, avoid repeated and Nucleocytoplasmic Transport. Neuron. Apr 5; 94 (1):48- handling and multiple freeze/thaw 57, 2017. cycles. Product shipped on dry ice.
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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.