JNK2 Antibody Cat. No.: 62-957

JNK2 Antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

This JNK2 (MAPK9) antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 349-379 amino acids from the C-terminal region of human JNK2 (MAPK9).

TESTED APPLICATIONS: IHC-P, WB

For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:50~100

PREDICTED MOLECULAR 48 kDa WEIGHT:

September 27, 2021 1 https://www.prosci-inc.com/jnk2-antibody-62-957.html Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: MAPK9

Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated ALTERNATE NAMES: protein kinase 1a, SAPK1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, MAPK9, JNK2, PRKM9, SAPK1A

ACCESSION NO.: P45984

PROTEIN GI NO.: 85700366

GENE ID: 5601

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

JNK2 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between BACKGROUND: binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. JNK2 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. It is inhibited by dual specificity phosphatases, such as DUSP1. The protein has been shown to bind to at least three scaffolding , MAPK8IP1/JIP-1, MAPK8IP2/JIP-2 and MAPK8IP3/JIP-3/JSAP1. These proteins also bind other components of the JNK signaling pathway

REFERENCES: 1) Gupta, S., et al., EMBO J. 15(11):2760-2770 (1996).

September 27, 2021 2 https://www.prosci-inc.com/jnk2-antibody-62-957.html 2) Sluss, H.K., et al., Mol. Cell. Biol. 14(12):8376-8384 (1994).

3) Kallunki, T., et al., Dev. 8(24):2996-3007 (1994).

4) Fleming, Y., et al., Biochem. J. 352 Pt 1, 145-154 (2000).

ANTIBODIES FOR RESEARCH USE ONLY.

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