Catalogue # Aliquot Size

P09-35G-20 20 µg P09-35G-50 50 µg

PCTK1 Protein Recombinant human protein expressed in Sf9 cells

Catalog # P09-35G

Lot # R169 -2

Product Description Purity

Recombinant human PCTK1(107-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_006201 .

The purity was determined to be Gene Aliases >95% by densitometry. Approx. MW 62 kDa . FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE

Formulation

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Storage and Stability

Store product at –70 oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background

PCTK1 or Pctaire protein kinase 1 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases which play a main role in signal transduction cascades in terminally differentiated cells, in exocytosis and in transport of secretory cargo from the endoplasmic PCTK1 Protein reticulum. PCTK1 is ubiquitously expressed with the highest Recombinant human protein expressed in Sf9 cells levels detected in the brain and testis (1). PCTK1 maps distal to the t(X;18) synovial sarcoma breakpoint in Catalog Number P09-35G Xp11.23. A 420-kb YAC clone positive for PCTK1 also Specific Lot Number R169-2 contained the gene coding for ubiquitin-activating Purity >95% enzyme UBE1 previously mapped to Xp11.3. (2). PCTK1 is Concentration 0.1 µ g/ µl Stability 1yr At –70 oC from date of shipment thought to escape X inactivation. Storage & Shipping Store product at –70 oC. For optimal storage, aliquot target into smaller References quantities after centrifugation and store at recommended temperature. 1. Okuda, T. et.al: PCTAIRE-1 and PCTAIRE-3, two members of a For most favorable performance, avoid repeated handling and multiple novel cdc2/CDC28-related protein kinase gene family. freeze/thaw cycles. Product shipped Oncogene 7: 2249-2258, 1992. on dry ice. 2. Knight, J. C. et.al: Physical linkage of the cdc2-related gene (PCTK1) and the ubiquitin-activating enzyme E1 gene (UBE1) on human Xp11.3. Cytogenet. Cell Genet. 71: 155-157, 1995.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.