6th EUROPEAN WORKSHOP ON PLANT CHROMATIN 13-14 June 2019
Max Planck Institute for Plant Breeding Research, Cologne
Scientific Organisers:
Claudia Köhler SLU, Uppsala
Valérie Gaudin INRA, Versailles
Franziska Turck (local Organizer) MPIPZ, Cologne Thank you to our Sponsors: Dear participants,
On behalf of the organizing committee, I am pleased to welcome you to the 6th European Workshop on Plant Chromatin.
When the EWPC workshop series started in 2009 at the ETH Zürich, the initiators Valerie Gaudin, Claudia Köhler and Lars Hennig wanted to provide a networking platform to young researchers that were just setting up groups The company of Biologists or new projects in the emerging field of plant chromatin regulation in Europe. The goal was to promote collaborations and personal connections. Indeed, several collective grant applications and joint publications have had their origins at an EWPC, testifying the success of the workshop.
The EWPC has grown into an open biannual 2-day series that has Deutsche been hosted by different European research institutions. Importantly, Forschungsgemeinschaft the EWPC remains the only established series on the topic Plant Chromatin in the European Research Area and as such has become a fixed date in the calendar of many former attendees. At the MPIPZ, we feel very privileged to have been selected to host the 6th EWPC.
Over the years, the EWPC has remained a forum in which younger researchers can make their entry to the research community in a relatively personal atmosphere. As the number of registrations increased over the years, short poster talks were introduced in 2015; in 2017 the event was further Diagenode enhanced by an invited keynote speaker. We have kept these traditions going and are very pleased to welcome Julie Law from the SALK Institute to give a keynote lecture that will be open to all researchers working at the MPIPZ. Furthermore, we are lucky to have the space and facilities at the MPIPZ that allowed us to add an actual poster session to the 6th EWPC and we are looking forward to lively poster lunch discussions.
Dispendix Sadly, Lars Hennig passed away last year. We will honour his memory in a session dedicated to his contribution a peer, mentor and friend. As local organiser, I have to thank many helping hands, from my group and the MPIPZ administration and technical staff, who have made this event possible. We are also very grateful to our sponsors from institutions and companies, whose contributions have considerably enhanced the quality of the event. ZEISS Research Microscopy I wish us all two exciting days on our favourite research topic and Solutions hope that we will all meet again at the 7th EWPC in 2021!
Franziska Turck Program
Thursday, 13. June 2019 08:45 – 9:00 Opening remarks
Session 1: A view on chromatin, techniques and nuclear structure Chair: Daniel Schubert
09:00 - 09:15 Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis
Célia Baroux (Zürich University, CH)
09:15 - 09:30 Ribosomal RNA genes distribution and expression organize nucleolus-associated chromatin domains
Frederic Pontvianne (CNRS LGDP, Perpignan, FR)
09:30 - 09:45 Elucidating the role of epigenetic regulators at the nuclear periphery in Arabidopsis
Kalyanikrishna (Freie Universität Berlin, DE) 09:45 - 10:00 Epigenetic pattern of plant telomeres with non-canonical sequence
Katerina Adamusova (CEITEC, Brno, CZ) 10:00 - 10:15 AT-hook proteins increase histone modification H3K9me2 i ndependently of DNA methylation
Hua Jiang (IPK, Gatersleben, DE) 10:15 - 10:20 RuvBL1 and RuvBL2a, the plant Pontin and Reptin homologues, are involved in telomerase biogenesis and regulation via TRB Proteins
Šárka Schořová (CEITEC, Brno, CZ) 10:20 - 10:25 CRISPR-based approaches to address PRC2 functions in Arabidopsis
Annick Dubois (ENS Lyon, FR) 10:25 - 10:30 Engineered DNA-binding molecule-mediated chromatin immunoprecipitation: a powerful tool to detect the 3D genome architecture in protoplasts
Tingting Yang (MPIPZ, Köln, DE)
EWPC 2019 | 1 6th European Workshop on Plant Chromatin, 13-14 June 2019 Program
Coffee break 14:30 - 14:45 Resetting epigenetic memory in the plant paternal genome 10:30 – 11:00 Coffee and more Michael Borg (GMI, Wien, AT) Keynote lecture 14:45 - 15:00 Identification of novel components of the MEDEA-containing Chair: Claudia Köhler complexes and their targets Sara Simonini (University of Zurich, CH) 11:00 - 12:00 Locus- and tissue-specific control of DNA methylation in 15:00 - 15:15 mHi-C reveals distinct chromatin architecture in endosperm and Arabidopsis thaliana leaf of Arabidopsis Julie Law (SALK, La Jolla, USA) Vikash Kumar Yadav (SLU, Uppsala, SE) 15:15 - 15:30 Interference with CG DNA methylation by a novel RdDM mutant in Arabidopsis Poster Lunch I 12:00 - 14:00 Finger food and Posters Gergely Molnár (BOKU, Tulin, AT) 15:30 - 15:45 Ploidy-dependent paramutation in Arabidopsis thaliana
Session 2: Chromatin, inheritance and generation changes Heinrich Bente (GMI, Wien, AT) Chair: Ortun Mittelsten Scheid Coffee break 14:00 - 14:05 Understanding functions of STRUCTURAL MAINTENANCE OF 15:45 - 16:15 Coffee and cake CHROMOSOMES 5/6 complex during generative development Fen Yang (CAS, Olomouc – Holice, CZ) Session 3: Chromatin variations 14:05 - 14:10 Evolutionary conservation of the Polycomb repressive complex Chair: Jiří Fajkus 2 (PRC2) and its role in photoautotropic growth in the moss Physcomitrella patens Katerina Kabelacova (CAS, České Budějovice, CZ) 16:15 - 16:30 Histone H3.3 deposition in Arabidopsis 14:10 - 14:15 NAP- deletion suppresses fas1 mutant phenotype and enhances Aline V. Probst (GReD, Clermont-Ferrand, FR) genome stability 16:30 - 16:45 The repressive role of Arabidopsis H2A.Z in transcriptional Martina Nešpor Dadejová (CEITEC, Brno, CZ) regulation depends on AtBMI1 activity 14:15 - 14:20 The genotypic impact on the rate of spontaneous epimutations in Wiam Merini (IBVF, Seville, Spain) Arabidopsis thaliana 16:45 - 17:00 Arabidopsis YAF9 histone readers modulate flowering time Rashmi R. Hazarika (TUM, Freising, DE) through NuA4-complex-dependent H4 and H2A.Z histone acetylation at FLC chromatin 14:20 - 14:25 Evolutionary and functional impact of epigenetic variation in forest trees (EPITREE) José A. Jarillo (UPM-INIA, Madrid, ES) Stéphane Maury (Orléans University-INRA, FR) 17:00 - 17:15 NASPSIM3 functions as a CenH3 chaperone in Arabidopsis 14:25 - 14:30 Evolution of DNA methylation profiles in Cape Verde Arabidopsis Inna Lermontova (CEITEC, Brno, CZ) Johan Zicola (MPIPZ, Köln, DE)
2 | EWPC 2019 EWPC 2019 | 3 6th European Workshop on Plant Chromatin, 13-14 June 2019 Program
17:15 - 17:30 Circuit of phosphorylations events on histone H2A variants Friday, 14. June 2019 during DNA damage response in Arabidopsis Session 4: A tribute to Lars Hennig Anna Schmücke (GMI, Wien, AT) Chair: Valerie Gaudin 17:30 - 17:35 A conserved protein complex acetylates histone H4 to enable chloroplast development 09:00 - 09:25 New tricks for old dogs - repurposing transposases for host Tomasz Bieluszewski (Adam Mickiewicz University, Poznan, PL) epigenetic regulation 17:35 - 17:40 The SSRP1 subunit of the FACT histone chaperone: Requirement Justin Goodrich (University Edinburgh, UK) of the HMG-box domain and role in anthocyanin induction 09:25 - 09:40 ATRX and chromatin remodeling Philipp Michl-Holzinger (University of Regensburg, DE) Sylvain Bischof (University of Zürich, CH) 17:40 - 17:45 Isolation and characterization of negative regulators of heat stress memory 09:40 - 09:55 PRC2 modulates response to light during the embryo-to-seedling transition in Arabidopsis Inês Trindade (University of Potsdam, DE) Iva Mozgová (Biologické centrum, České Budějovice, CZ) 17:45 - 17:50 Tissue-specific investigations upon transposable element movement into a glucosinolate transporter 09:55 - 10:10 The Arabidopsis methylome is robust to dark-induced senescence Emma Aller (Copenhagen University, DK) Minerva S. Trejo-Arellano (SLU, Uppsala, SE) 17:50 - 17:55 PICKLE RELATED 1 regulates floral transition of Arabidopsis 10:10 - 10:20 Role of chromatin-bound DEK proteins in the regulation of PRC2 environmental insensitive quintuple mutant soc1 svp flc ft tsf target genes Alice Pajoro (MPIPZ, DE) Miyuki Nakamura (SLU, Uppsala, SE) 17:55 - 18:00 SR45 regulates FLOWERING LOCUS C through RNA polymerase II 10:20 - 10:30 UBIQUITIN SPECIFIC PROTEASES 12 and 13 remove H2Aub1 for checkpoint activity in Arabidopsis stable repression of responsive genes Philip Wolff (JIC, Norwich, UK) Lejon E. M. Kralemann (SLU, Uppsala, SE) Coffee break 10:30 – 11:00 Coffee and cake Shuttle bus to City Center Dinner Session 5: An open view on chromatin 19:00 – 21:00 Kölsch Brewery culture and scientific discussions Chair: Klaus Grasser Brauhaus Sion, Unter Taschenmacher 5-7, Köln 11:00 - 11:15 Lateral organ founder cells display cell type specific chromatin accessibility patterns within the Arabidopsis inflorescence meristem Julia Engelhorn (University Düsseldorf, DE) 11:15 - 11:30 Ubiquitination machineries acting at the interface between light signaling and epigenome dynamics in plants Sandra Fonseca (CNB-CSIC, Madrid, ES)
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11:30 - 11:45 Role of the HMGAs proteins in chromatin dynamics and 15:00 - 15:15 How to control transcriptional output? How VAL1 and VAL1- transcription interactors limit FLC expression. Simon Amiard (GRED, Clermont-Ferrand, FR) Pawel Mikulski ( JIC, Norwich, UK) 11:45 - 12:00 Arabidopsis S2Lb links AtCOMPASS-like and SDG2 activity in 15:15 - 15:30 Chromatin features predicting immunity-related transcription histone H3 Lys-4 trimethylation independently from histone H2B reprogramming in Arabidopsis monoubiquitination Dmitry Lapin (MPIPZ, Köln, DE Clara Bourbousse (IBENS, Paris, FR) 15:30 - 15:45 Functional characterization of Super determinant 1 (Sde1) 12:00 - 12:05 Understanding and harnessing inter-individual gene expression suggests an epigenetic regulation of axillary meristem initiation variability in Arabidopsis thaliana in Tomato Sandra Cortijo (Sainsbury Laboratory CU, UK) Hernan Lopez-Marin (MPIPZ, Köln, DE) 12:05 - 12:10 Bromodomains are essential for the functions of SWI/SNF 15:45 – 16:00 Concluding remarks and discussion complex in Arabidopsis Rafal Archacki (University of Warsaw, PL) Shuttle bus to train stations 12:10 - 12:15 Investigating the involvement of TELOMERE REPEAT BINDING 16:00 – 16:30 Shuttle bus to train station Bahnhof-Ehrenfeld protein mediated gene regulation in photosynthesis Ailbhe Jane Brazel (MPIPZ, Köln, DE) Poster Lunch II 12:15 - 14:00 Finger food and Posters
Session 6: Chromatin and Regulation Chair: Manuel Piñeiro
14:00 - 14:15 ULTRAPETALA: at the crossroads of chromatin regulators? Cristel Carles (Université Grenoble, FR) 14:15 - 14:30 Interplay between co-transcriptional chromatin states and transcript isoform diversity Sebastian Marquardt (Copenhagen University, DK) 14:30 - 14:45 High-throughput precision phenotyping of a large panel of A. thaliana epigenetic hybrids reveals widespread heterosis in developmental traits Ioanna Kakoulidou (TUM, Freising, DE) 14:45 - 15:00 PWO1 INTERACTORS (PWIs) interact and co-localize with components of the PcG pathway Sara Farrona (National University of Ireland Galway, IE)
6 | EWPC 2019 EWPC 2019 | 7 6th European Workshop on Plant Chromatin, 13-14 June 2019 List of Participants
List of Participants Bente, Heinrich Gregor Mendel Institute [email protected] Adamusova, Katerina Mendel Centre for Plant Genomics and Proteomics, CEITEC, Masaryk University Bieluszewski, Tomasz [email protected] Department of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Aller, Emma [email protected] Copenhagen University [email protected] Bischof, Sylvain University of Zürich Amelung, Lisa [email protected] Universität Hamburg - Institut für Pflanzenwissenschaften und Mikrobiologie [email protected] Borg, Michael Gregor Mendel Institute Amiard, Simon [email protected] GRED - Université Clermont Auvergne [email protected] Brazel, Ailbhe Max Planck Institute for Plant Breeding Research Archacki, Rafal [email protected] University of Warsaw [email protected] Calonje, Myriam Institute of Plant Biochemistry and Photosynthesis (IBVF-CSIC) Atanassov, Hristo [email protected] Centre Hospitalier Universitaire de Poitiers, FRANCE [email protected] Carles, Christel Université Grenoble Alpes Atanassova, Rossitza [email protected] University of Poitiers, FRANCE [email protected] Cerise, Martina University of Milan, Fabio Fornara Laboratory Barneche, Fredy [email protected] IBENS - CNRS [email protected] Cortijo, Sandra Sainsbury Laboratory Cambridge University Baroux, Célia [email protected] Institute of Plant and Microbial Biology, University of Zürich [email protected] Coupland, George Max Planck Institute for Plant Breeding Research Becker, Claude [email protected] Gregor Mendel Institute of Molecular Plant Biology [email protected]
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Creppe, Catherine Gigolashvili, Tamara Diagenode University of Cologne [email protected] [email protected]
Crevillén, Pedro Goodrich, Justin Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) University of Edinburgh crevillé[email protected] [email protected]
Dubois, Annick Grasser, Klaus RDP Lab, Ecole Normale Superieure de Lyon University of Regensburg [email protected] [email protected]
Engelhorn, Julia Hazarika, Rashmi Rekha Max Planck Institute for Plant Breeding Research Technical University of Munich [email protected] [email protected]
Fajkus, Petr Jacobs, Chean Sern Institute of Biophysics of the CAS, v. v. i. RDP, ENS de Lyon, France [email protected] [email protected]
Fajkus, Jiří Jariilo, Jose Antonio CEITEC, Masaryk University Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de [email protected] Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Farrona, Sara [email protected] National University of Ireland Galway [email protected] Jiang, Hua The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Fojtova, Miloslava [email protected] CEITEC Masaryk University Brno [email protected] Kabelacova, Katerina Institute of Plant Molecular Biology Fonseca, Sandra [email protected] Centro Nacional de Biotecnologia (CNB-CSIC) [email protected] Kakoulidou, Ioanna Technical University of Munich Gaudin, Valérie [email protected] INRA [email protected] Kalyanikrishna, Kalyanikrishna Free University of Berlin [email protected]
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Köhler, Claudia Manuel, Piñeiro Linnean Center of Plant Biology Instituto Nacional de Investigaciones Agrarias [email protected] [email protected]
Kralemann, Lejon Marquardt, Sebastian Swedish University of Agricultural Sciences (SLU) University of Copenhagen [email protected] [email protected]
Krause, Kristin Maury, Stéphane Max Planck Institute for Plant Breeding Research Université Orléans INRA [email protected] [email protected]
Kumar, Amit Mehrotra, Rajesh Indian Institute of Science Education and Research (IISER) - Pune Birla Institue of Technology and science [email protected] [email protected]
Lapin, Dmitry MERINI, WIAM Max Planck Institue for Plant Breeding Research Institute of Plant Biochemistry and Photosynthesis, Seville, Spain [email protected] [email protected]
Law, Julie Michl-Holzinger, Philipp SALK Institute University of Regensburg [email protected] [email protected]
Lermontova, Inna Mikulski, Pawel Central European Institute of Technology (CEITEC), Masaryk University, Leibniz John Innes Centre Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben [email protected] [email protected] Mitreiter, Simon Lopez-Marin, Hernan Dario University of Cologne Max Planck Institute for Plant Breeding Research [email protected] [email protected] Mittelsten Scheid, Ortrun Maas, Lena Gregor Mendel Institute Wageningen University and Research [email protected] [email protected] Mohan, Rajinikanth Mangilet, Anchilie Colgate University Uni Oldenburg [email protected] [email protected]
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Molnar, Gergely Pratx, Loris Dept. of Applied Genetics and Cell Biology, University of Natural Resources and Potsdam University Life Sciences (BOKU) [email protected] [email protected] Probst, Aline Moreno-Romero, Jordi GReD, CNRS UMR6293, Université Clermont Auvergne, INSERM U1103 Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB [email protected] [email protected] Randall, Ricardo Mozgova, Iva University of Zürich Biologické centrum AV ČR, v. v. i. [email protected] [email protected] Richet-Bourbousse, Clara Nakamura, Miyuki IBENS The Swedish University of Agricultural Sciences [email protected] [email protected] Rubio, Vicente Nešpor Dadejová, Martina National Center of Biotechnology-CSIC Central European Institute of Technology (CEITEC) and Faculty of Sciences of [email protected] Masaryk University in Brno [email protected] Rutowicz, Kinga University of Zurich, Institute of Plant and Microbial Biology Pajoro, Alice [email protected] Max Planck Institute for Plant Breeding Research [email protected] Sandmann, Michael Freie Universität Berlin Pernickova, Katerina [email protected] Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research Schmücker, Anna [email protected] Gregor-Mendel-Institute [email protected] Philippe, Johann to Berens IBMP - Institut de biologie moléculaire des plantes - CNRS UPR 2357 Schořová, Šárka [email protected] Faculty of Science, Masaryk University/CEITEC [email protected] Pontvianne, Frederic CNRS LGDP Schubert, Daniel [email protected] Freie Universität Berlin [email protected] Poza-Viejo, Laura Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) [email protected]
94 | EWPC 2019 EWPC 2019 | 95 6th European Workshop on Plant Chromatin, 13-14 June 2019 List of Participants
Shen, Wen Hui Tsiantis, Miltos IBMP-CNRS Max Planck Institute for Plant Breeding Research [email protected] [email protected]
Siemons, Charlotte Turck, Franziska Wageningen University & Research Max Planck Institute for Plant Breeding Research [email protected] [email protected]
Simonini, Sara Tyshenko, Anastasiya University of Zurich SFedU [email protected] [email protected]
Srivastava, Anjil Kumar Vachon, Gilles Durham University CNRS [email protected] [email protected]
Sykorova, Eva Varotto, Serena Institute of Biophysics University of Padova [email protected] [email protected]
Theres, Klaus von Pein, Linn Max Planck Institute for Plant Breeding Research Institute for plant science and microbiology, University of Hamburg [email protected] [email protected]
Theune, Marius Lasse Wolff, Philip Botanisches Institut CAU-Kiel John Innes Centre [email protected] [email protected]
Thomson, Bennett Yadav, Vikash Smurfit Institute of Genetics Swedish University of Agricultural Sciences [email protected] [email protected]
Trejo-Arellano, Minerva Susana Yang, Tingting SLU Max Planck Institute for Plant Breeding Research [email protected] [email protected] Yang, Fen Trindade, Ines Institute of Experimental Botany CAS University of Potsdam [email protected] [email protected] Zhou, Haoran Max Planck Institute for Plant Breeding Research [email protected]
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Zicola, Johan Max Planck Institute for Plant Breeding Research [email protected]
Zündorf, Simone Max Planck Institute for Plant Breeding Research [email protected]
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Cover illustration and poster: Franziska Turck, MPIPZ Layout abstract book: Britta Hoffmann, MPIPZ
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author
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