SANTA CRUZ BIOTECHNOLOGY, INC. TMCO1 shRNA Plasmid (h): sc-88439-SH

BACKGROUND STORAGE AND RESUSPENSION TMCO1 (transmembrane and coiled-coil domain-containing 1), also Store lyophilized shRNA plasmid DNA at 4° C with desiccant. Stable for known as TMCC4 (transmembrane and coiled-coil domains protein 4) or xeno- at least one year from the date of shipment. Once resuspended, store at geneic cross-immune protein PCIA3, is a 188 amino acid multi-pass mem- 4° C for short term storage or -80° C for long term storage. Avoid repeated brane protein that belongs to the TMCO1 family and exists as two alternative- freeze thaw cycles. ly spliced isoforms. While expressed abundantly in thymus, prostate, testis Resuspend lyophilized shRNA plasmid DNA in 200 µl of the deionized water and small intestine, TMCO1 is expressed to a lower extent in brain, placenta, provided. Resuspension of the shRNA plasmid DNA in 200 µl of deionized lung and kidney. The that encodes TMCO1 contains approximately water makes a 0.1 µg/µl solution in a 10 mM Tris, 1 mM EDTA buffered 44,890 bases and maps to human 1q24.1. Comprising nearly 8% solution. of the , spans 260 million base pairs, contains over 3,000 and houses a large number of disease-associated genes, APPLICATIONS including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher TMCO1 shRNA Plasmid (h) is recommended for the inhibition of TMCO1 syndrome. expression in human cells.

REFERENCES SUPPORT REAGENTS 1. Watson, M.L., Kingsmore, S.F., Johnston, G.I., Siegelman, M.H., Le Beau, For optimal shRNA Plasmid transfection efficiency, Santa Cruz Biotechnology’s M.M., Lemons, R.S., Bora, N.S., Howard, T.A., Weissman, I.L., McEver, shRNA Plasmid Transfection Reagent: sc-108061 (0.2 ml) and shRNA Plasmid R.P., et al. 1990. Genomic organization of the selectin family of leukocyte Transfection Medium: sc-108062 (20 ml) are recommended. Control shRNAs adhesion molecules on human and mouse chromosome 1. J. Exp. Med. are available as 20 µg lyophilized plasmid DNA. Each encodes a scrambled 172: 263-272. shRNA sequence that will not lead to the specific degradation of any known cellular mRNA. Control shRNA Plasmids include: sc-108060, sc-108065 and 2. Blackwood, D.H., Fordyce, A., Walker, M.T., St. Clair, D.M., Porteous, D.J. sc-108066. and Muir, W.J. 2001. Schizophrenia and affective disorders — cosegrega- tion with a translocation at chromosome 1q42 that directly disrupts brain- RT-PCR REAGENTS expressed genes: clinical and P300 findings in a family. Am. J. Hum. Genet. 69: 428-433. Semi-quantitative RT-PCR may be performed to monitor TMCO1 gene expres- sion knockdown using RT-PCR Primer: TMCO1 (h)-PR: sc-88439-PR (20 µl, 3. Weise, A., Starke, H., Mrasek, K., Claussen, U. and Liehr, T. 2005. New 409 bp). Annealing temperature for the primers should be 55-60° C and the insights into the evolution of chromosome 1. Cytogenet. Genome Res. 108: extension temperature should be 68-72° C. 217-222. 4. Lans, H. and Hoeijmakers, J.H. 2006. Cell biology: aging nucleus gets out RESEARCH USE of shape. Nature 440: 32-34. The purchase of this product conveys to the buyer the nontransferable right 5. Gregory, S.G., Barlow, K.F., McLay, K.E., Kaul, R., Swarbreck, D., Dunham, to use the purchased amount of the product and all replicates and derivatives A., Scott, C.E., Howe, K.L., Woodfine, K.C., Spencer, C.A., Jones, M.C., for research purposes conducted by the buyer in his laboratory only (whether Gillson, C., Searle, S., Zhou, Y., Kokocinski, F., McDonald, L., et al. 2006. the buyer is an academic or for-profit entity). The buyer cannot sell or other- The DNA sequence and biological annotation of human chromosome 1. wise transfer (a) this product (b) its components or (c) materials made using Nature 441: 315-321. this product or its components to a third party, or otherwise use this product or its components or materials made using this product or its components CHROMOSOMAL LOCATION for Commercial Purposes. Genetic locus: TMCO1 (human) mapping to 1q24.1. PROTOCOLS PRODUCT See our web site at www.scbt.com for detailed protocols and support products. TMCO1 shRNA Plasmid (h) is a pool of 3 target-specific lentiviral vector plasmids each encoding 19-25 nt (plus hairpin) shRNAs designed to knock down gene expression. Each plasmid contains a puromycin resistance gene for the selection of cells stably expressing shRNA. Each vial contains 20 µg of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also see TMCO1 siRNA (h): sc-88439 and TMCO1 shRNA (h) Lentiviral Particles: sc-88439-V as alternate gene silencing products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com