DOCSLIB.ORG

Download Available)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

Distribution Agreement In presenting this thesis as a partial fulfillment of the requirements for a degree from Emory University, I hereby grant to Emory University and its agents the non-exclusive license to archive, make accessible, and display my thesis in whole or in part in all forms of media, now or hereafter now, including display on the World Wide Web. I understand that I may select some access restrictions as part of the online submission of this thesis. I retain all ownership rights to the copyright of the thesis. I also retain the right to use in future works (such as articles or books) all or part of this thesis. Stephanie Pintas April 1, 2018 Antifungal Activity of Botanical Extracts against Malassezia furfur Implicated in Common Skin Conditions By Stephanie Pintas Dr. Cassandra Quave Adviser Department of Dermatology Dr. Cassandra Quave Adviser Dr. Meleah Hickman Committee Member Dr. Sarah Chisolm Committee Member 2018 Antifungal Activity of Botanical Extracts against Malassezia furfur Implicated in Common Skin Conditions by Stephanie Pintas Dr. Cassandra Quave Adviser An abstract of a thesis submitted to the Faculty of Emory College of Arts and Sciences of Emory University in partial fulfillment of the requirements of the degree of Bachelor of Arts with Honors Human Health Department 2018 Abstract Antifungal Activity of Botanical Extracts against Malassezia furfur Implicated in Common Skin Conditions By Stephanie Pintas Malassezia furfur is a commensal lipophilic fungus found on the surface of the skin that is implicated in the pathogenesis of several prevalent skin conditions, including Atopic Dermatitis (AD), Seborrheic Dermatitis (SD), and Tinea Versicolor (TV). With antifungal resistance becoming an increasing concern, it is important to identify new therapeutic options. While several studies have demonstrated anti-Malassezia activity with botanical extracts, the concentration tested is often high (>500 µg/mL) and not evaluated for mammalian cytotoxicity. The purpose of this study was to screen for potential anti-Malassezia activity of botanical extracts using a unique chemical library, the Quave Natural Products Library (QNPL), based on plants used in the traditional treatment of infectious and inflammatory skin disease. Initial screen of the QNPL (~1000 extracts) was conducted at 16 µg/mL, but failed to demonstrate anti- Malassezia activity. A second screen of the library was conducted at 128 µg/mL by broth microtiter plate dilution method in triplicate. The screen revealed potential activity for crude extracts 31, 55, 66, 637, and 1251 and pure compounds 448, 449, and 451. Further analysis via two-fold serial dilution in quadruplicate demonstrated 48-hour MIC90 at 128 µg/mL for extract 1251, Allium amethystinum, and 16 µg/mL for pure compound 451 as compared to 2 µg/mL for Ketoconazole control. Minimum fungicidal concentration (MFC) was determined by agar plate test. MFC was 256 µg/mL for both pure compound 451 and extract 1251. We also conducted two-fold serial dilution on extracts 158 and 237, ethanolic and methanolic crude extracts of Allium cepa (onion) in the same genus as A. amethystinum, but failed to observe any growth inhibition at either 24 or 48-hour optical density reads. To our knowledge, this is the first report of antifungal activity of Allium amethystinum. Mammalian cytotoxicity as determined by testing of human keratinocytes (HaCaTs) via LDH assay revealed that extract 1251 did not achieve an IC50 in HaCaTs at 512 µg/mL, twice the concentration that exhibited fungicidal activity. This provides promising evidence for potential topical treatments that include Allium amethystinum. Chemical inhibition of Malassezia furfur at lesional skin sites could support reduced allergenic responses and inflammation, improving symptoms of disease and decreasing risk of fungal resistance from current topical treatments. Future efforts to isolate the active compound(s) of extract 1251 via iterative bioassay guided fractionation are warranted. Antifungal Activity of Botanical Extracts against Malassezia furfur Implicated in Common Skin Conditions by Stephanie Pintas Dr. Cassandra Quave Adviser A thesis submitted to the Faculty of Emory College of Arts and Sciences of Emory University in partial fulfillment of the requirements of the degree of Bachelor of Arts with Honors Human Health Department 2018 Acknowledgements The idea for my thesis all began because of my own struggles with atopic dermatitis. I grew increasingly frustrated with the inefficacy of topical steroids at relieving my symptoms long-term. I started doing personal research online and found several “natural” remedies such as tea tree oil and apple cider vinegar. However, I wanted to put these ideas to the test and see if I could discover botanical extracts that truly exhibited antimicrobial effects in the lab. My hope is that this research will provide insights and information for future clinical testing and topical natural products that may help individuals who also suffer from chronic skin conditions. I would like to express my sincere appreciation and gratitude to Dr. Quave, for supporting my research, guiding me through this immense process, and pushing me to learn more about botanical medicine than I could ever imagine. Without her guidance and expertise, this project would not have been possible, and I am so appreciative that she was willing to accept me into her lab to pursue this project. I would also like to thank my thesis committee members, Dr. Chisolm, for providing knowledge on the skin conditions I researched and Dr. Hickman for her interest and expertise in fungal microbes. I would like to especially thank Kate Nelson for teaching me the microbiology techniques necessary for my experiments and Dr. Lyles for his expertise in phytochemistry. A huge thank you to Dr. Freeman for providing her support and endless tips for creating a thorough thesis and presentation. I am thankful to my parents, especially my mother who sparked my interest in this topic because of its clinical relevance in dermatology. I would also like to thank my father, grandparents, and brothers for supporting and encouraging me throughout the process. TABLE OF CONTENTS Chapter 1 Introduction........................................................................................................... 1 1.1 Skin Microbiome .................................................................................................................. 1 1.2 Characteristics of Malassezia spp. ........................................................................................ 7 1.3 Atopic Dermatitis .................................................................................................................. 9 1.4 The Role of Malassezia species in Atopic Dermatitis ........................................................ 11 1.5 Seborrheic Dermatitis ......................................................................................................... 16 1.6 Tinea Versicolor.................................................................................................................. 20 Chapter 2 Background ......................................................................................................... 22 2.1 Antifungal Resistance and Toxicity: The Need for New Solutions .................................... 22 2.2 Implications of Other Treatments ....................................................................................... 28 2.3 Current Research on Botanical Extracts ............................................................................. 31 2.4 In Vitro Research against Malassezia species .................................................................... 32 2.5 Clinical Research Utilizing Botanical Extracts................................................................... 42 2.6 Relevance of Studying Botanicals ...................................................................................... 52 Chapter 3 Materials and Methods....................................................................................... 53 3.1 Collection and Extraction ................................................................................................... 53 3.2 Growing Malassezia species: Agar and Broth Microdilution Method ............................... 56 3.3 Microbiological Assay: Initial Screen ................................................................................ 57 3.4 Serial Dilution and Fungicidal Plate Test ........................................................................... 59 3.5 XTT Assay .......................................................................................................................... 62 3.6 Cytotoxicity Assay .............................................................................................................. 66 Chapter 4 Results .................................................................................................................. 67 4.1 Screening Outcomes ........................................................................................................... 67 4.2 Antifungal Activities—MIC Results .................................................................................. 92 4.3 Fungicidal Plate Results ...................................................................................................... 98 4.4 LDH Cytotoxicity Results................................................................................................. 102 Chapter 5 Discussion
Recommended publications
  • Discovery of Anti-Amoebic Inhibitors from Screening the MMV Pandemic Response Box on Balamuthia Mandrillaris, Naegleria Fowleri

    Discovery of Anti-Amoebic Inhibitors from Screening the MMV Pandemic Response Box on Balamuthia Mandrillaris, Naegleria Fowleri

    bioRxiv preprint doi: https://doi.org/10.1101/2020.05.14.096776; this version posted May 15, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Article 2 Discovery of anti-amoebic inhibitors from screening 3 the MMV Pandemic Response Box on Balamuthia 4 mandrillaris, Naegleria fowleri and Acanthamoeba 5 castellanii 6 Christopher A. Rice1,2,Δ,†,*, Emma V. Troth2,3,†, A. Cassiopeia Russell2,3,†, and Dennis E. Kyle1,2,3,* 7 1 Department of Cellular Biology, University of Georgia, Athens, Georgia, USA. 8 2 Center for Tropical and Emerging Global Diseases, Athens, Georgia, USA. 9 3 Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA. 10 Δ Current address: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, 11 University of Georgia, Athens, Georgia, USA. 12 † These authors contributed equally to this work. 13 14 *Author correspondence: [email protected] (D.E.K) and [email protected] (C.A.R) 15 Received: date; Accepted: date; Published: date 16 Abstract: Pathogenic free-living amoebae, Balamuthia mandrillaris, Naegleria fowleri and several 17 Acanthamoeba species are the etiological agents of severe brain diseases, with case mortality rates 18 >90%. A number of constraints including misdiagnosis and partially effective treatments lead to 19 these high fatality rates. The unmet medical need is for rapidly acting, highly potent new drugs to 20 reduce these alarming mortality rates. Herein, we report the discovery of new drugs as potential 21 anti-amoebic agents.
  • Integrated Molecular Profiling for Analyzing and Predicting Therapeutic Mechanism, Response, Biomarker and Target

    Integrated Molecular Profiling for Analyzing and Predicting Therapeutic Mechanism, Response, Biomarker and Target

    INTEGRATED MOLECULAR PROFILING FOR ANALYZING AND PREDICTING THERAPEUTIC MECHANISM, RESPONSE, BIOMARKER AND TARGET Jia Jia (B. Sci & M. Sci, Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF PHARMACY NATIONAL UNIVERSITY OF SINGAPORE 2010 Acknowledgements ACKNOWLEDGEMENTS I would like to deeply thank Professor Chen Yu Zong, for his constant encouragement and advice during the entire period of my postgraduate studies. In particular, he has guided me to make my research applicable to the real world problem. This work would not have been possible without his kindness in supporting me to shape up the manuscript for publication. I am also tremendously benefited from his profound knowledge, expertise in scientific research, as well as his enormous support, which will inspire and motivate me to go further in my future professional career. I am also grateful to our BIDD group members for their insight suggestions and collaborations in my research work: Dr. Tang Zhiqun, Ms. Ma Xiaohua, Mr. Zhu Feng, Ms. Liu Xin, Ms. Shi Zhe, Dr. Cui Juan, Mr. Tu Weimin, Dr. Zhang Hailei, Dr. Lin Honghuang, Dr. Liu Xianghui, Dr. Pankaj Kumar, Dr Yap Chun wei, Ms. Wei Xiaona, Ms. Huang Lu, Mr. Zhang Jinxian, Mr. Han Bucong, Mr. Tao Lin, Dr. Wang Rong, Dr. Yan Kun. I thank them for their valuable support and encouragement in my work. Finally, I owe my gratitude to my parents for their forever love, constant support, understanding, encouragement and strength throughout my life. A special appreciation goes to all for love and support. Jia Jia August 2010 I Table of Contents TABLE OF CONTENTS 1.1 Overview of mechanism and strategies of molecular-targeted therapeutics ...................................
  • 1,4-Disubstituted-1,2,3-Triazole Compounds Induce Ultrastructural Alterations in Leishmania Amazonensis Promastigote

    1,4-Disubstituted-1,2,3-Triazole Compounds Induce Ultrastructural Alterations in Leishmania Amazonensis Promastigote

    International Journal of Molecular Sciences Article 1,4-Disubstituted-1,2,3-Triazole Compounds Induce Ultrastructural Alterations in Leishmania amazonensis Promastigote: An in Vitro Antileishmanial and in Silico Pharmacokinetic Study Fernando Almeida-Souza 1,2,* , Verônica Diniz da Silva 3,4, Gabriel Xavier Silva 5, Noemi Nosomi Taniwaki 6, Daiana de Jesus Hardoim 2, Camilla Djenne Buarque 3, 1, , 2, Ana Lucia Abreu-Silva * y and Kátia da Silva Calabrese y 1 Pós-graduação em Ciência Animal, Universidade Estadual do Maranhão, São Luís 65055-310, Brazil 2 Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; [email protected] (D.d.J.H.); calabrese@ioc.fiocruz.br (K.d.S.C.) 3 Laboratório de Síntese Orgânica, Pontifícia Universidade Católica, Rio de Janeiro 22451-900, Brazil; [email protected] (V.D.d.S.); [email protected] (C.D.B.) 4 Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2825-149 Caparica, Portugal 5 Rede Nordeste de Biotecnologia, Universidade Federal do Maranhão, São Luís 65080-805, Brazil; [email protected] 6 Núcleo de Microscopia Eletrônica, Instituto Adolfo Lutz, São Paulo 01246-000, Brazil; [email protected] * Correspondence: [email protected] (F.A.-S.); [email protected] (A.L.A.-S.) These authors equally contributed to this work. y Received: 26 June 2020; Accepted: 14 July 2020; Published: 18 September 2020 Abstract: The current standard treatment for leishmaniasis has remained the same for over 100 years, despite inducing several adverse effects and increasing cases of resistance. In this study we evaluated the in vitro antileishmanial activity of 1,4-disubstituted-1,2,3 triazole compounds and carried out in silico predictive study of their pharmacokinetic and toxicity properties.
  • Malassezia Species Associated with Dermatitis in Dogs and Their Antifungal Susceptibility

    Malassezia Species Associated with Dermatitis in Dogs and Their Antifungal Susceptibility

    Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 1994-2007 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 06 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.706.236 Malassezia Species Associated With Dermatitis in Dogs and Their Antifungal Susceptibility Uday Seetha, Sumanth Kumar, Raghavan Madhusoodanan Pillai, Mouttou Vivek Srinivas*, Prabhakar Xavier Antony and Hirak Kumar Mukhopadhyay Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Pondicherry-605 009, India *Corresponding author ABSTRACT The present study was taken with the objective of isolation, characterization, molecular detection and antifungal sensitivity of Malassezia species from dermatitis cases from dogs in and around Pondicherry state. A total of 100 skin swabs were collected from the dogs K e yw or ds showing dermatological problems suggestive of Malassezia. Out of 100 swabs, 41 Malassezia isolates were successfully isolated and had good growth on Sabouraud’s Malassezia pachydermatis, Dextrose Agar (SDA) during the primary isolation from the skin swabs. Biochemical tests Sabouraud’s dextrose agar, Modified Dixon’s agar, for catalase, β- glucosidase activities and the capability to grow with three water soluble Polymerase chain reaction, lipid supplements, namely Tween 20, Tween 80 and Cremophor EL concluded that the M. Antifungal Susceptibility testing pachydermatis was the sole species isolated from the cases of canine dermatitis in Pondicherry state. Cytological examination revealed that direct skin swab smear was more Article Info sensitive than adhesive tape technique and impression smear. The frequency of isolation of Accepted: M. pachydermatis was higher in neck region (8) followed by other regions in canine.
  • Review Article Sporotrichosis: an Overview and Therapeutic Options

    Review Article Sporotrichosis: an Overview and Therapeutic Options

    Hindawi Publishing Corporation Dermatology Research and Practice Volume 2014, Article ID 272376, 13 pages http://dx.doi.org/10.1155/2014/272376 Review Article Sporotrichosis: An Overview and Therapeutic Options Vikram K. Mahajan Department of Dermatology, Venereology & Leprosy, Dr. R. P. Govt. Medical College, Kangra, Tanda, Himachal Pradesh 176001, India Correspondence should be addressed to Vikram K. Mahajan; [email protected] Received 30 July 2014; Accepted 12 December 2014; Published 29 December 2014 Academic Editor: Craig G. Burkhart Copyright © 2014 Vikram K. Mahajan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Sporotrichosis is a chronic granulomatous mycotic infection caused by Sporothrix schenckii, a common saprophyte of soil, decaying wood, hay, and sphagnum moss, that is endemic in tropical/subtropical areas. The recent phylogenetic studies have delineated the geographic distribution of multiple distinct Sporothrix species causing sporotrichosis. It characteristically involves the skin and subcutaneous tissue following traumatic inoculation of the pathogen. After a variable incubation period, progressively enlarging papulo-nodule at the inoculation site develops that may ulcerate (fixed cutaneous sporotrichosis) or multiple nodules appear proximally along lymphatics (lymphocutaneous sporotrichosis). Osteoarticular sporotrichosis or primary pulmonary sporotrichosis are rare and occur from direct inoculation or inhalation of conidia, respectively. Disseminated cutaneous sporotrichosis or involvement of multiple visceral organs, particularly the central nervous system, occurs most commonly in persons with immunosuppression. Saturated solution of potassium iodide remains a first line treatment choice for uncomplicated cutaneous sporotrichosis in resource poor countries but itraconazole is currently used/recommended for the treatment of all forms of sporotrichosis.
  • Flora Mediterranea 26

    Flora Mediterranea 26

    FLORA MEDITERRANEA 26 Published under the auspices of OPTIMA by the Herbarium Mediterraneum Panormitanum Palermo – 2016 FLORA MEDITERRANEA Edited on behalf of the International Foundation pro Herbario Mediterraneo by Francesco M. Raimondo, Werner Greuter & Gianniantonio Domina Editorial board G. Domina (Palermo), F. Garbari (Pisa), W. Greuter (Berlin), S. L. Jury (Reading), G. Kamari (Patras), P. Mazzola (Palermo), S. Pignatti (Roma), F. M. Raimondo (Palermo), C. Salmeri (Palermo), B. Valdés (Sevilla), G. Venturella (Palermo). Advisory Committee P. V. Arrigoni (Firenze) P. Küpfer (Neuchatel) H. M. Burdet (Genève) J. Mathez (Montpellier) A. Carapezza (Palermo) G. Moggi (Firenze) C. D. K. Cook (Zurich) E. Nardi (Firenze) R. Courtecuisse (Lille) P. L. Nimis (Trieste) V. Demoulin (Liège) D. Phitos (Patras) F. Ehrendorfer (Wien) L. Poldini (Trieste) M. Erben (Munchen) R. M. Ros Espín (Murcia) G. Giaccone (Catania) A. Strid (Copenhagen) V. H. Heywood (Reading) B. Zimmer (Berlin) Editorial Office Editorial assistance: A. M. Mannino Editorial secretariat: V. Spadaro & P. Campisi Layout & Tecnical editing: E. Di Gristina & F. La Sorte Design: V. Magro & L. C. Raimondo Redazione di "Flora Mediterranea" Herbarium Mediterraneum Panormitanum, Università di Palermo Via Lincoln, 2 I-90133 Palermo, Italy [email protected] Printed by Luxograph s.r.l., Piazza Bartolomeo da Messina, 2/E - Palermo Registration at Tribunale di Palermo, no. 27 of 12 July 1991 ISSN: 1120-4052 printed, 2240-4538 online DOI: 10.7320/FlMedit26.001 Copyright © by International Foundation pro Herbario Mediterraneo, Palermo Contents V. Hugonnot & L. Chavoutier: A modern record of one of the rarest European mosses, Ptychomitrium incurvum (Ptychomitriaceae), in Eastern Pyrenees, France . 5 P. Chène, M.
  • Tohum Çimlenme Hizinin Ekolojik Önemi Ve Farkli Çimlenme Hizi Hesaplama Yöntemlerinin Karşilaştirilmasi

    Tohum Çimlenme Hizinin Ekolojik Önemi Ve Farkli Çimlenme Hizi Hesaplama Yöntemlerinin Karşilaştirilmasi

    TOHUM ÇİMLENME HIZININ EKOLOJİK ÖNEMİ VE FARKLI ÇİMLENME HIZI HESAPLAMA YÖNTEMLERİNİN KARŞILAŞTIRILMASI THE ECOLOGICAL IMPORTANCE OF THE GERMINATION RATE AND THE COMPARISON OF VARIOUS GERMINATION RATE ESTIMATION METHODS CİHAN ÜNAL DEĞİRMENCİ DOÇ. DR. ÇAĞATAY TAVŞANOĞLU Tez Danışmanı Hacettepe Üniversitesi Lisansüstü Eğitim-Öğretim ve Sınav Yönetmeliğinin Biyoloji Anabilim Dalı için Öngördüğü YÜKSEK LİSANS TEZİ olarak hazırlanmıştır. 2019 ÖZET TOHUM ÇİMLENME HIZININ EKOLOJİK ÖNEMİ VE FARKLI ÇİMLENME HIZI HESAPLAMA YÖNTEMLERİNİN KARŞILAŞTIRILMASI Cihan Ünal Değirmenci Yüksek Lisans, Biyoloji Bölümü Tez Danışmanı: Doç. Dr. Çağatay Tavşanoğlu Haziran 2019, x + 61 sayfa Akdeniz havzasının coğrafi konumu, iklim tipi ve kendi içindeki değişkenliği, insan etkisi, yangının yaygın bir etmen olması ve bitki çeşitliliği bölgeyi bitki ekolojisi çalışmaları için ideal bir alan yapmaktadır. Bu tez çalışmasında, Akdeniz havzasında yapılmış olan çalışmaların bulguları ve verileri kullanılarak çimlenme hızı verileri içeren bir veri tabanı oluşturulmuştur. Bu veri tabanı kullanılarak çimlenme hızının sıcaklık şoku ve duman uygulamaları ile inkübasyon sıcaklığından nasıl etkilendiği incelenmiştir. Bitkilerin büyüme şekli, sürgün verme yeteneği ve familyasının çimlenme hızı üzerindeki etkileri de çalışma kapsamında araştırılmıştır. Çalışmanın sonucunda, büyüme şeklinin Akdeniz Havzası bitkilerinde çimlenme hızının önemli bir belirleyicisi olduğu ve tek yıllık bitkilerin çok yıllık otsu ve odunsu türlere göre daha hızlı çimlendiği bulunmuştur. Büyüme şekli
  • Federico Selvi a Critical Checklist of the Vascular Flora of Tuscan Maremma

    Federico Selvi a Critical Checklist of the Vascular Flora of Tuscan Maremma

    Federico Selvi A critical checklist of the vascular flora of Tuscan Maremma (Grosseto province, Italy) Abstract Selvi, F.: A critical checklist of the vascular flora of Tuscan Maremma (Grosseto province, Italy). — Fl. Medit. 20: 47-139. 2010. — ISSN 1120-4052. The Tuscan Maremma is a historical region of central western Italy of remarkable ecological and landscape value, with a surface of about 4.420 km2 largely corresponding to the province of Grosseto. A critical inventory of the native and naturalized vascular plant species growing in this territory is here presented, based on over twenty years of author's collections and study of relevant herbarium materials and literature. The checklist includes 2.056 species and subspecies (excluding orchid hybrids), of which, however, 49 should be excluded, 67 need confirmation and 15 have most probably desappeared during the last century. Considering the 1.925 con- firmed taxa only, this area is home of about 25% of the Italian flora though representing only 1.5% of the national surface. The main phytogeographical features in terms of life-form distri- bution, chorological types, endemic species and taxa of particular conservation relevance are presented. Species not previously recorded from Tuscany are: Anthoxanthum ovatum Lag., Cardamine amporitana Sennen & Pau, Hieracium glaucinum Jord., H. maranzae (Murr & Zahn) Prain (H. neoplatyphyllum Gottschl.), H. murorum subsp. tenuiflorum (A.-T.) Schinz & R. Keller, H. vasconicum Martrin-Donos, Onobrychis arenaria (Kit.) DC., Typha domingensis (Pers.) Steud., Vicia loiseleurii (M. Bieb) Litv. and the exotic Oenothera speciosa Nutt. Key words: Flora, Phytogeography, Taxonomy, Tuscan Maremma. Introduction Inhabited by man since millennia and cradle of the Etruscan civilization, Maremma is a historical region of central-western Italy that stretches, in its broadest sense, from south- ern Tuscany to northern Latium in the provinces of Pisa, Livorno, Grosseto and Viterbo.
  • Malassezia Baillon, Emerging Clinical Yeasts

    Malassezia Baillon, Emerging Clinical Yeasts

    FEMS Yeast Research 5 (2005) 1101–1113 www.fems-microbiology.org MiniReview Malassezia Baillon, emerging clinical yeasts Roma Batra a,1, Teun Boekhout b,*, Eveline Gue´ho c, F. Javier Caban˜es d, Thomas L. Dawson Jr. e, Aditya K. Gupta a,f a Mediprobe Research, London, Ont., Canada b Centraalbureau voor Schimmelcultures, Uppsalalaan 8, 85167 Utrecht, The Netherlands c 5 rue de la Huchette, F-61400 Mauves sur Huisne, France d Departament de Sanitat i dÕ Anatomia Animals, Universitat Auto`noma de Barcelona, Bellaterra, Barcelona E-08193, Spain e Beauty Care Technology Division, Procter & Gamble Company, Cincinnati, USA f Division of Dermatology, Department of Medicine, Sunnybrook and WomenÕs College Health Science Center (Sunnybrook site) and the University of Toronto, Toronto, Ont., Canada Received 1 November 2004; received in revised form 11 May 2005; accepted 18 May 2005 First published online 12 July 2005 Abstract The human and animal pathogenic yeast genus Malassezia has received considerable attention in recent years from dermatolo- gists, other clinicians, veterinarians and mycologists. Some points highlighted in this review include recent advances in the techno- logical developments related to detection, identification, and classification of Malassezia species. The clinical association of Malassezia species with a number of mammalian dermatological diseases including dandruff, seborrhoeic dermatitis, pityriasis ver- sicolor, psoriasis, folliculitis and otitis is also discussed. Ó 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Keywords: Malassezia; Yeast; Identification; Animals; Disease 1. Introduction a positive staining reaction with Diazonium Blue B (DBB) [3]. The genus was named in 1889 by Baillon Members of the genus Malassezia are opportunistic [6] with the species M.
  • Plant Species Richness and Composition of a Habitat Island

    Plant Species Richness and Composition of a Habitat Island

    Biodiversity Data Journal 8: e48704 doi: 10.3897/BDJ.8.e48704 Research Article Plant species richness and composition of a habitat island within Lake Kastoria and comparison with those of a true island within the protected Pamvotis lake (NW Greece) Alexandros Papanikolaou‡‡, Maria Panitsa ‡ Division of Plant Biology, Department of Biology, University of Patras, Patras, Greece Corresponding author: Maria Panitsa ([email protected]) Academic editor: Gianniantonio Domina Received: 22 Nov 2019 | Accepted: 07 Jan 2020 | Published: 15 Jan 2020 Citation: Papanikolaou A, Panitsa M (2020) Plant species richness and composition of a habitat island within Lake Kastoria and comparison with those of a true island within the protected Pamvotis lake (NW Greece). Biodiversity Data Journal 8: e48704. https://doi.org/10.3897/BDJ.8.e48704 Abstract Lake Kastoria is one of the potentially “ancient” Balkan lakes that has a great environmental importance and ecological value, attracts high touristic interest and is under various anthropogenic pressures. It belongs to a Natura 2000 Special Protection Area and a Site of Community Interest. The city of Kastoria is located at the western part of the lake and just next to it, towards the centre of the lake, is a peninsula, a habitat island. In the framework of research concerning the flora of lake islands of Greece, one of the main objectives of the present study is to fill a gap concerning plant species richness of the habitat island within the protected Lake Kastoria, which is surrounded by the lake except for its north-western part where the border of the city of Kastoria is located.
  • Janis Ruksans, Dr.Biol.H.C. Late Summer/Autumn 2006 Bulb Nursery Box 2, P.O

    Janis Ruksans, Dr.Biol.H.C. Late Summer/Autumn 2006 Bulb Nursery Box 2, P.O

    Janis Ruksans, Dr.biol.h.c. Late summer/autumn 2006 Bulb Nursery Box 2, P.O. ROZULA LV-4150 Cesis distr. LATVIA /fax +371 – 41-33-223 +371 - 941-84-40, 41-00-326 All prices for single bulb E-mail: [email protected] in EURO Dear friends! New Year and new gardening season comes and my new catalogue goes to you. It is a little shorter than usually - not for shortage of items includable (with pain in heart I striped out many names) but this summer I want to rebuild my bulb shed and it will limit my possibilities to harvest bulbs and to work with them. Last summer was very busy year. I rebuilt one of my greenhouses - it was very nervous process due shortage of workers (three teams were changed), I was forced to dig out all bulbs, to change soil - but now working in it is much more comfortable. I and my wife Guna organized for the first time “Open door days” and we had 18 visitors from Britain, arranged programm including lectures, visits of gardens, museum, nature, Opera etc. All visitors were very satisfied with visit. Sorry, this season we can’t to arrange OPEN DOOR DAYS due my lecture tour in North America and expedition plans. But I’m planning repeat such days in future, too. Last season Mrs. Kristl Walek from Canada (Gardens North – Seeds from the world) in her catalogue revealed the fact that I had been bearing in my mind an idea about a book on bulbs and my experience with them.
  • (12) United States Patent (10) Patent No.: US 6,767,552 B2 Narang (45) Date of Patent: Jul

    (12) United States Patent (10) Patent No.: US 6,767,552 B2 Narang (45) Date of Patent: Jul

    USOO6767552B2 (12) United States Patent (10) Patent No.: US 6,767,552 B2 Narang (45) Date of Patent: Jul. 27, 2004 (54) ADHESIVE TREATMENT FOR ORAL 5.530,037 A 6/1996 McDonnell et al. FUNGAL INFECTION 5,554,365 A 9/1996 Byram et al. 5,575.997 A 11/1996 Leung et al. (75) Inventor: Upvan Narang, Raleigh, NC (US) 5,580,565 A 12/1996 Tighe et al. 5,582,834 A 12/1996 Leung et al. (73) Assignee: Closure Medical Corporation, 5,613,942 A 3/1997 Lucast et al. Raleigh, NC (US) 5,624,669 A 4/1997 Leung et al. 9, 5,653,769 A 8/1997 Barley, Jr. et al. 5,661,170 A 8/1997 Chodosh (*) Notice: Subject to any disclaimer, the term of this 5,665,817 A 810: E. al. patent is extended or adjusted under 35 5,684,042 A 11/1997 Greff et al. U.S.C. 154(b) by 359 days. 5,716,607 A 2/1998 Byram et al. 5,717.005 A 2/1998 Richardson (21) Appl. No.: 09/898,092 5,725,491 A 3/1998 Tipton et al. 5,730,994. A 3/1998 Askill et al. (22) Filed: Jul. 5, 2001 5,753,699 A 5/1998 Greff et al. 5,755,680 A 5/1998 Ghodsian (65) Prior Publication Data 5,762.919 A 6/1998 Greff et al. 5,762.955 A 6/1998 Smith US 2003/0007947 A1 Jan. 9, 2003 5,783,177 A 7/1998 Greff et al.