CONTENTS • APRIL 2020 • VOLUME 94, NO. 7

COVER IMAGE Cover photograph: HIV-1 viral membrane fusion is mediated through binding of HIV-1 envelope () glycoprotein to CD4, which results in recruitment of coreceptor CCR5 or CXCR4. NF279 or NF449 can inhibit HIV-1 productive infection through inhibition of HIV-1 viral membrane fusion, shown through antagonism of the V1V2 region of HIV-1 Env (dark green dots). The P2X1 receptor is shown nearby in the membrane, and NF279 or NF449 may directly inhibit the receptor in addition to antagonism of HIV-1 Env. (See related article in March 2020, vol. 94, no. 6, e01622-19.) (Courtesy of Ni-ka Ford, Mount Sinai Health System; reprinted with permission.)

SPOTLIGHT Rozanne M. R Articles of Significant Interest in This Issue e00152-20 Sandri-Goldin Spotlight

STRUCTURE AND ASSEMBLY Jae U. Jung T Phase Separation of Epstein-Barr Virus EBNA2 and Its Coactivator EBNALP e01771-19 Structure Controls Gene Expression Qiu Peng, Lujuan Wang, Zailong Qin, Jia Wang, Xiang Zheng, Lingyu Wei, Xiaoyue Zhang, Xuemei Zhang, Can Liu, Zhengshuo Li, Yangge Wu, Guiyuan Li, Qun Yan, Jian Ma Susana Lopez T Conserved NSP5 and VP2 Domains Interact and Affect e01965-19 Structure Antonino Buttafuoco, Kevin Michaelsen, Kurt Tobler, Mathias Ackermann, Cornel Fraefel, Catherine Eichwald

GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION Frank Kirchhoff T Genetic Variability of Long Terminal Repeat Region between HIV-2 Groups e01504-19 Replication Impacts Transcriptional Activity Quentin Le Hingrat, Benoit Visseaux, Mélanie Bertine, Lise Chauveau, Olivier Schwartz, Fidéline Collin, Florence Damond, Sophie Matheron, Diane Descamps, Charlotte Charpentier, on behalf of the ANRS HIV-2 CO5 Cohort Richard M. T B Cell-Specific Transcription Activator PAX5 Recruits p300 To Support e02028-19 Longnecker Replication EBNA1-Driven Transcription Cheng-Der Liu, Hsueh-Ling Lee, Chih-Wen Peng

GENETIC DIVERSITY AND EVOLUTION Julie K. Pfeiffer T Seroreactive Profiling of Filoviruses in Chinese Bats Reveals Extensive Infection e02042-19 Diversity & Evol of Diverse Viruses Chang Zhang, Zhongyi Wang, Jianqiu Cai, Xiaomin Yan, Fuqiang Zhang, Jianmin Wu, Lin Xu, Zongzheng Zhao, Tingsong Hu, Changchun Tu, Biao He

VIRUS-CELL INTERACTIONS Tom Gallagher T Receptor Recognition by the Novel Coronavirus from Wuhan: an Analysis Based e00127-20 Interactions on Decade-Long Structural Studies of SARS Coronavirus Yushun Wan, Jian Shang, Rachel Graham, Ralph S. Baric, Fang Li

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Frank Kirchhoff T -Labelled HIV To Investigate the Role of Capsid during Nuclear Import e01024-19 Interactions and Integration Irena Zurnic Bönisch, Lieve Dirix, Veerle Lemmens, Doortje Borrenberghs, Flore De Wit, Frank Vernaillen, Susana Rocha, Frauke Christ, Jelle Hendrix, Johan Hofkens, Zeger Debyser Viviana Simon T A Conserved Acidic-Cluster Motif in SERINC5 Confers Partial Resistance to e01554-19 Interactions Antagonism by HIV-1 Charlotte A. Stoneham, Peter W. Ramirez, Rajendra Singh, Marissa Suarez, Andrew Debray, Christopher Lim, Xiaofei Jia, Yong Xiong, John Guatelli Stacey T PARP1 Enhances Influenza A Virus Propagation by Facilitating Degradation of e01572-19 Schultz-Cherry Interactions Host Type I Interferon Receptor Chuan Xia, Jennifer J. Wolf, Chuankai Sun, Mengqiong Xu, Caleb J. Studstill, Jun Chen, Hanh Ngo, Hua Zhu, Bumsuk Hahm Mark T. Heise T Sensitivity of Alphaviruses to G3BP Deletion Correlates with Efficiency of e01681-19 Interactions Replicase Polyprotein Processing Benjamin Götte, Age Utt, Rennos Fragkoudis, Andres Merits, Gerald M. McInerney Viviana Simon T Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive e01708-19 Interactions Binding of Core-Binding Factor Beta Eri Miyagi, Sarah Welbourn, Sayaka Sukegawa, Helena Fabryova, Sandra Kao, Klaus Strebel Guido Silvestri T The Late Domain of Prototype Foamy Virus Gag Facilitates Autophagic Clearance e01719-19 Interactions of Stress Granules by Promoting Amphisome Formation Yingcheng Zheng, Guoguo Zhu, Jun Yan, Yinglian Tang, Song Han, Jun Yin, Biwen Peng, Xiaohua He, Wanhong Liu Mark T. Heise T A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose e01751-19 Interactions Synthase Complex as a Host Dependency Factor for Dengue Virus Infection Athena Labeau, Etienne Simon-Loriere, Mohamed-Lamine Hafirassou, Lucie Bonnet-Madin, Sarah Tessier, Alessia Zamborlini, Thierry Dupré, Nathalie Seta, Olivier Schwartz, Marie-Laure Chaix, Constance Delaugerre, Ali Amara, Laurent Meertens Felicia Goodrum T Human Cytomegalovirus Decreases Major Histocompatibility Complex Class II by e01901-19 Interactions Regulating Class II Transactivator Transcript Levels in a Myeloid Cell Line Praneet K. Sandhu, Nicholas J. Buchkovich Richard M. T Nascent Transcriptomics Reveal Cellular Prolytic Factors Upregulated Upstream e01966-19 Longnecker Interactions of the Latent-to-Lytic Switch Protein of Epstein-Barr Virus Tiffany R. Frey, Jozan Brathwaite, Xiaofan Li, Sandeepta Burgula, Ibukun A. Akinyemi, Saurabh Agarwal, Eric M. Burton, Mats Ljungman, Michael T. McIntosh, Sumita Bhaduri-McIntosh Viviana Simon T HIV-1 Vpu Downregulates Tim-3 from the Surface of Infected CD4؉ T Cells e01999-19 Interactions Jérémie Prévost, Cassandra R. Edgar, Jonathan Richard, Steven M. Trothen, Rajesh Abraham Jacob, Mitchell J. Mumby, Suzanne Pickering, Mathieu Dubé, Daniel E. Kaufmann, Frank Kirchhoff, Stuart J. D. Neil, Andrés Finzi, Jimmy D. Dikeakos Richard M. T Roles of the Different Isoforms of the Pseudorabies Virus Protein Kinase pUS3 in e02029-19 Longnecker Interactions Nuclear Egress Julia Sehl, Sandy Pörtner, Barbara G. Klupp, Harald Granzow, Kati Franzke, Jens P. Teifke, Thomas C. Mettenleiter

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Frank Kirchhoff T Two Functional Variants of AP-1 Complexes Composed of either ␥2or␥1 e02039-19 Interactions Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef Lucas A. Tavares, Julianne V. de Carvalho, Cristina S. Costa, Roberta M. Silveira, Andreia N. de Carvalho, Eduardo A. Donadi, Luis L. P. daSilva Rebecca Ellis T Typical Stress Granule Proteins Interact with the 3= Untranslated Region of e02041-19 Dutch Interactions Enterovirus D68 To Inhibit Viral Replication Jinyan Cheng, Shuai Gao, Cheng Zhu, Sihua Liu, Jinyu Li, Jun Kang, Zhiyun Wang, Tao Wang Stacey T Elongin C Contributes to RNA Polymerase II Degradation by the Interferon e02134-19 Schultz-Cherry Interactions Antagonist NSs of La Crosse Orthobunyavirus Andreas Schoen, Simone Lau, Paul Verbruggen, Friedemann Weber Rozanne M. T Virus Glycoproteins A33, A34, and B5 Form a Complex for Efficient e02155-19 Sandri-Goldin Interactions Endoplasmic Reticulum to trans-Golgi Network Transport Stephanie R. Monticelli, Amalia K. Earley, Raychel Stone, Christopher C. Norbury, Brian M. Ward

CELLULAR RESPONSE TO INFECTION Jae U. Jung T Human Epitopes Identified from Virus Tegument Protein VP11/12 e01991-19 Response ؉ (UL46) Recall Multifunctional Effector Memory CD4 TEM Cells in Asymptomatic Individuals and Protect from Ocular Herpes Infection and Disease in “Humanized” HLA-DR Transgenic Mice Ruchi Srivastava, Pierre-Gregoire A. Coulon, Swayam Prakash, Nisha R. Dhanushkodi, Soumyabrata Roy, Angela M. Nguyen, Nuha I. Alomari, Uyen T. Mai, Cassendra Amezquita, Caitlin Ye, Bernard Maillère, Lbachir BenMohamed

VACCINES AND ANTIVIRAL AGENTS Mark T. Heise T Rapid Generation of Attenuated Infectious Bursal Disease Virus from e01569-19 Vaccines Dual-Promoter Plasmids by Reduction of Viral Ribonucleoprotein Activity Hui Yang, Yu Wang, Chengjin Ye Frank Kirchhoff T Autologous Antibody Responses to an HIV Envelope Glycan Hole Are Not Easily e01861-19 Vaccines Broadened in Rabbits Yuhe R. Yang, Laura E. McCoy, Marit J. van Gils, Raiees Andrabi, Hannah L. Turner, Meng Yuan, Christopher A. Cottrell, Gabriel Ozorowski, James Voss, Matthias Pauthner, Thomas M. Polveroni, Terrence Messmer, Ian A. Wilson, Rogier W. Sanders, Dennis R. Burton, Andrew B. Ward Guido Silvestri T Glycan Profiles of gp120 Protein Vaccines from Four Major HIV-1 Subtypes e01968-19 Vaccines Produced from Different Host Cell Lines under Non-GMP or GMP Conditions Shixia Wang, Yegor Voronin, Peng Zhao, Mayumi Ishihara, Nickita Mehta, Mindy Porterfield, Yuxin Chen, Christopher Bartley, Guangnan Hu, Dong Han, Lance Wells, Michael Tiemeyer, Shan Lu Richard M. T Evasion of a Human Cytomegalovirus Entry Inhibitor with Potent Cysteine Reactivity e02012-19 Longnecker Vaccines Is Concomitant with the Utilization of a Heparan Sulfate Proteoglycan-Independent Route of Entry M. J. Murray, N. I. Bonilla-Medrano, Q. L. Lee, S. J. Oxenford, R. Angell, D. P. Depledge, M. B. Reeves

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Joanna L. Shisler T Development of a Highly Effective African Swine Fever Virus Vaccine by Deletion e02017-19 Vaccines of the I177L Gene Results in Sterile Immunity against the Current Epidemic Eurasia Strain Manuel V. Borca, Elizabeth Ramirez-Medina, Ediane Silva, Elizabeth Vuono, Ayushi Rai, Sarah Pruitt, Lauren G. Holinka, Lauro Velazquez-Salinas, James Zhu, Douglas P. Gladue Colin R. Parrish T Identification and Characterization of Novel Compounds with Broad-Spectrum e02149-19 Vaccines Antiviral Activity against Influenza A and B Viruses Jun-Gyu Park, Ginés Ávila-Pérez, Aitor Nogales, Pilar Blanco-Lobo, Juan C. de la Torre, Luis Martínez-Sobrido

PATHOGENESIS AND IMMUNITY Adolfo T Hemagglutinin and Neuraminidase Antibodies Are Induced in an Age- and e01385-19 García-Sastre Pathogenesis Subtype-Dependent Manner after Influenza Virus Infection Sook-San Wong, Ben Waite, Jacqui Ralston, Tim Wood, G. Edwin Reynolds, Ruth Seeds, E. Claire Newbern, Mark G. Thompson, Q. Sue Huang, Richard J. Webby, the SHIVERS Investigation Team Julie K. Pfeiffer T Genetic Determinants of Altered Virulence of Type O Foot-and-Mouth e01657-19 Pathogenesis Disease Virus Fan Yang, Zixiang Zhu, Weijun Cao, Huanan Liu, Ting Wei, Min Zheng, Keshan Zhang, Ye Jin, Jijun He, Jianhong Guo, Xiangtao Liu, Haixue Zheng J.-H. James Ou T Discovery and Selection of -Derived T Cell Epitopes for e01663-19 Pathogenesis Global Immunotherapy Based on Viral Indispensability, Conservation, and HLA-Binding Strength Monique T. A. de Beijer, Diahann T. S. L. Jansen, Yingying Dou, Wim J. E. van Esch, Juk Yee Mok, Mariëlle J. P. Maas, Giso Brasser, Robert A. de Man, Andrea M. Woltman, Sonja I. Buschow Bryan RG T Thioredoxin 2 Negatively Regulates Innate Immunity to RNA Viruses by e01756-19 Williams Pathogenesis Disrupting the Assembly of the Virus-Induced Signaling Adaptor Complex Dan Li, Wenping Yang, Yi Ru, Jingjing Ren, Xiangtao Liu, Haixue Zheng Mark T. Heise T A Factor I-Like Activity Associated with Chikungunya Virus Contributes to Its e02062-19 Pathogenesis Resistance to the Human Complement System Joydeep Nag, Reshma Koolaparambil Mukesh, Sreenath Muraleedharan Suma, Umerali Kunnakkadan, Nisha Asok Kumar, John Bernet Johnson

ERRATUM Rozanne M. X Erratum for Rak et al., “Human Cytomegalovirus UL135 Interacts with Host e00748-19 Sandri-Goldin Erratum Adaptor Proteins To Regulate Epidermal Growth Factor Receptor and Reactivation from Latency” Michael A. Rak, Jason Buehler, Sebastian Zeltzer, Justin Reitsma, Belen Molina, Scott Terhune, Felicia Goodrum

AUTHOR CORRECTION Rozanne M. X Correction for Shakya et al., “Interferon Gamma Inhibits Varicella-Zoster Virus e02138-19 Sandri-Goldin Correction Replication in a Cell Line-Dependent Manner” Akhalesh K. Shakya, Dennis J. O’Callaghan, Seong K. Kim

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