SAN TA C RUZ BI OTEC HNOL OG Y, INC . GSE1 (G-6): sc-515046

BACKGROUND APPLICATIONS 16 encodes over 900 in approximately 90 million base GSE1 (G-6) is recommended for detection of GSE1 of mouse, rat and human pairs, makes up nearly 3% of human cellular DNA and is associated with a origin by Western Blotting (starting dilution 1:100, dilution range 1:100- variety of genetic disorders. The GAN is located on 1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total (1 ml and, with mutation, may lead to giant axonal neuropathy, a nervous system of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range disorder characterized by increasing malfunction with growth. The rare dis - 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range or der Rubinstein-Taybi syndrome is also associated with chromosome 16, 1:30- 1:3000). though through the CREBBP gene which encodes a critical CREB binding Suitable for use as control antibody for GSE1 siRNA (h): sc-93036, GSE1 protein. Signs of Rubinstein-Taybi include mental retardation and predisposi - siRNA (m): sc-145800, GSE1 shRNA Plasmid (h): sc-93036-SH, GSE1 shRNA tion to tumor growth and white blood cell neoplasias. Crohn’s disease is a Plasmid (m): sc-145800-SH, GSE1 shRNA (h) Lentiviral Particles: sc-93036-V gastrointestinal inflammatory condition associated with chromosome 16 and GSE1 shRNA (m) Lentiviral Particles: sc-145800-V. through the NOD2 gene. An association with systemic lupus erythematosis and a number of other autoimmune disorders with the pericentromeric region Molecular Weight of GSE1 isoforms: 136/129/126 kDa. of chromosome 16 has led to the identification of SLC5A11 as a potential Positive Controls: T-47D cell lysate: sc-2293, CCRF-CEM cell lysate: sc-2225 autoimmune modifier. The KIAA0182 gene product has been provisionally or MCF7 whole cell lysate: sc-2206. designated KIAA0182 pending further characterization. RECOMMENDED SUPPORT REAGENTS REFERENCES To ensure optimal results, the following support reagents are recommended: 1. Ben Hamida, C., et al. 1997. Homozygosity mapping of giant axonal neu - 1) Western Blotting: use m-IgG κ BP-HRP: sc-516102 or m-IgG κ BP-HRP (Cruz ropathy gene to chromosome 16q24.1. Neurogenetics 1: 129-133. Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ 2. Karlsson, J., et al. 2003. Novel quantitative trait loci controlling develop - Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: ment of experimental autoimmune encephalomyelitis and proportion of sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- lymphocyte subpopulations. J. Immunol. 170: 1019-1026. cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3) Immunofluorescence: use m-IgG BP-FITC: sc-516140 or m-IgG BP-PE: 3. Forabosco, P., et al. 2006. Meta-analysis of genome-wide linkage studies κ κ sc-516141 (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: of systemic lupus erythematosus. Genes Immun. 7: 609-614. sc-24941 or UltraCruz ® Hard-set Mounting Medium: sc-359850. 4. Carneiro, L.A., et al. 2007. Nod-like receptors in innate immunity and inflammatory diseases. Ann. Med. 39: 581-593. DATA

CHROMOSOMAL LOCATION ABC AB 210 K – Genetic locus: GSE1 (human) mapping to 16q24.1; Gse1 (mouse) mapping 210 K – to 8 E1. GSE1 GSE1 120 K – SOURCE 120 K – GSE1 (G-6) is a mouse monoclonal antibody raised against amino acids 86K– 86K– 257-336 mapping near the N-terminus of GSE1 of human origin.

GSE1 (G-6): sc-515046. Western blot analysis of GSE1 GSE1 (G-6): sc-515046. Western blot analysis ofGSE1 PRODUCT expression in CCRF-CEM (A), MDA-MB-231 (B) and expression in T-47D (A) and MCF7 (B) whole cell SK-BR-3 (C) whole cell lysates. lysates. Each vial contains 200 µg IgG 1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. STORAGE GSE1 (G-6) is available conjugated to agarose (sc-515046 AC), 500 µg/ Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of 0.25 ml agarose in 1 ml, for IP; to HRP (sc-515046 HRP), 200 µg/ml, for shipment. Non-hazardous. No MSDS required. WB, IHC(P) and ELISA; to either phycoerythrin (sc-515046 PE), fluorescein (sc-515046 FITC), Alexa Fluor ® 488 (sc-515046 AF488), Alexa Fluor ® 546 RESEARCH USE ® ® (sc-515046 AF546), Alexa Fluor 594 (sc-515046 AF594) or Alexa Fluor 647 For research use only, not for use in diagnostic procedures. (sc-515046 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Alexa Fluor ® 680 (sc-515046 AF680) or Alexa Fluor ® 790 (sc-515046 AF790), PROTOCOLS 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM. See our web site at www.scbt.com for detailed protocols and support Alexa Fluor ® is a trademark of Molecular Probes, Inc., Oregon, USA products.

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