For Research Use Only PSMD5 Polyclonal antibody

Catalog Number:11469-1-AP www.ptgcn.com

Basic Information Catalog Number: GenBank Accession Number: Recommended Dilutions: 11469-1-AP BC014478 WB 1:1000-1:8000 Size: GeneID (NCBI): IP 0.5-4.0 ug for IP and 1:500-1:2000 for WB 600 μg/ml 5711 IHC 1:20-1:200 Source: Full Name: Rabbit proteasome (prosome, macropain) 26S subunit, Isotype: non-ATPase, 5 IgG Calculated MW: Purification Method: 504 aa, 56 kDa Antigen affinity purification Observed MW: Immunogen Catalog Number: 50 kDa AG2026

Applications Tested Applications: Positive Controls: IHC, IP, WB,ELISA WB : HeLa cells; Jurkat cells, human liver tissue, Species Specificity: mouse liver tissue human, mouse, rat IP : mouse liver tissue; Note-IHC: suggested angen retrieval with TE buffer pH 9.0; (*) Alternavely, angen IHC : human prostate cancer tissue; retrieval may be performed with citrate buffer pH 6.0

PSMD5, also named as KIAA0072, belongs to the proteasome subunit S5B/HSM3 family. It acts as a chaperone during the assembly of the 26S proteasome, Background Information specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly, it is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a SMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. (PMID:15489334). This antibody is specific to PSMD5.

Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under T: 4006900926 E: [email protected] W: ptgcn.com Proteintech Group brand and is not available to purchase from any other manufacturer. Selected Validation Data

HeLa cells were subjected to SDS PAGE followed Immunohistochemical analysis of paraffin- IP result of anti-PSMD5 (IP:11469-1-AP, 4ug; by western blot with 11469-1-AP (PSMD5 embedded human prostate cancer using 11469-1- Detection:11469-1-AP 1:1000) with mouse liver antibody) at dilution of 1:5000 incubated at room AP (PSMD5 antibody) at dilution of 1:100 (under tissue lysate 4000 ug. temperature for 1.5 hours. 10x lens).