Lotus Newsletter (2003) Volume 33, 63 – 96.

Lotus literature Database provided by Lotus Newsletter recipients, last updated Nov. 30 2003

ALI S.I. and SOKOLOFF D.D. 2001. A new combination in Pseudolotus Rech. f. (Leguminosae: Loteae). Kew Bull., 56, 721-723. Pseudolotus is a genus segregated from Lotus.

ALLAN G.J., ZIMMER E.A., WAGNER W.L. and SOKOLOFF D.D. 2003. Molecular phylogenetic analyses of tribe Loteae (Leguminosae): implications for classification and biogeography. In: Klitgaard B.B. and Bruneau A. (eds.). Advances in Legume Systematics. Pt. 10, Higher Level Systematics. Kew: Royal Botanic Gardens, pp. 371-393. [In press]

ALTIER N. 2003. Caracterización de la población de Fusarium oxysporum y potencial patogénico del suelo bajo rotaciones agrícola ganaderas. In: Rotaciones. Montevideo, INIA. Serie Técnica, No.134, 37-44. [Altier N. 2003. Characterization of the population of Fusarium oxysporum and soil pathogenical potential under crop-pasture rotations. In: Rotations. Montevideo, INIA. . Serie Técnica, No.134, 37-44. [In Spanish]]. During 1996-2002, soil samplings were performed three times per year in a crop rotation experiment established in 1963 at INIA la Estanzuela, Uruguay. Soil population of Fusarium oxysporum and pathogen potential were estimated for four cropping systems differing in the pasture type (legume or grass-legume) and pasture duration (0-4 years). Recovery of F. oxysporum, the primary pathogen associated with crown and root rot of forage legumes, was proportional to the time that the soil remained under legume pasture. Average CFU/g of soil were 1678, 2967, 2990 and 3934, for 100% agriculture – no pasture, 50% agriculture – 50% birdsdfoot trefoil pasture, 50% agriculture – 50% grass-legume pasture and 33% agriculture – 66% red clover and grass-legume pasture, respectively. Isolates of F. oxysporum recovered from different systems were pathogenic to seed and seedlings of birdsfoot trefoil and red clover; however, significant differences in aggressiveness were observed among them. Isolate aggressiveness to birdsfoot trefoil was higher than to red clover, as expressed by percentage of surviving plants (28.7% and 58.6%, respectively). When sown in soil under legume pasture, birdsfoot trefoil productivity was significantly more depressed than red clover productivity, as expressed by emergence and total biomass. The highest pathogen potential to both legume species was recorded under the system with 50% birdsfoot trefoil pasture. We concluded that the crop sequence that characterizes each system has a long-term effect on the soil fungal populations. Thus, crop rotation needs to be considered as a means for disease management, which contributes toward legume persistence and sustainable cropping systems.

ALTIER N., PÉREZ C., DE LA FUENTE L. and ARIAS A. 2000. Native fluorescent Pseudomonas as biocontrol agents of seedling diseases on birdsfoot trefoil. Proceedings of the Fifth International PGPR Workshop. October 29 – November 3, 2000. Córdoba, Argentina. http://www.ag.auburn.edu/argentina/pdfmanuscripts/altier.pdf

ARANGO N., JACOBS B.C. and BLUMENTHAL M.J. 1998. Seed production of Lotus uliginosus

63 64 Lotus literature

cv. Sharnae in response to plant population density. Australian Journal of Experimental Agriculture, 38, 837-842.

ARCHER A.C., WILSON G.P.M. and WALKER R. 1981. Lotus species. NSW Agriculture Agfact P2.5.12, first edition.

ARMSTEAD I.P. and WEBB K.J. 1987. Effect of age and type of tissue on genetic transformation of Lotus corniculatus by Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture, 9, 95-101.

ARTOLA A., CARRILLO CASTAÑEDA G. and GARCÍA DE LOS SANTOS G. 2003. Hydropriming: a strategy to increase Lotus corniculatus L. seed vigor. Seed Science and Technology, 31, 455-463.

ARTOLA A., GARCÍA DE LOS SANTOS G. and CARRILLO CASTAÑEDA G. 2003. A seed vigor test for birdsfoot trefoil. Seed Science and Technology, 31, 753 -757.

AYRES J.F. 1997. Highlights of lotus grazing management research in NSW. Grassland Society of NSW Newsletter, 12, 20-21.

AYRES J.F. 1997. “Planned lotus planting can reap rewards”. Nornews Rural, September 1997, p 5.

AYRES J.F. 1999. Summary sheet – “Greater lotus”.

AYRES J.F. 2002. Co-learning in pasture R&D – the lotus project. Grassland Society of NSW Newsletter, 17, 18-21.

AYRES J.F. 2002. Co-learning in pasture R&D – the lotus project. District Agronomists Conference held at the Orange campus of the University of Sydney, 5-7 February 2002.

AYRES J.F. 2002. New lotus varieties for acid soils in northern NSW. Acid Soil Action Review Workshop, Agricultural Research Institute, Wagga Wagga, 2-4 September 2002.

AYRES J.F. and BLUMENTHAL M.J. 2000. Lotus grazing management for weaner production. Final Report on DAN 082 (1994/95 – 1998/99) for Meat & Livestock Australia

AYRES J.F. and BLUMENTHAL M.J. 2001. Lotus opens new opportunities for grazing. Farming Ahead 119 pp 58-59.

AYRES J.F., BLUMENTHAL M.J. and EARLY R. 2002. Lotus opens new opportunities for grazing. Grasslands Society of NSW Newsletter, 17, 12-14.

AYRES J.F., BLUMENTHAL M.J. and HOCHMAN Z. 1997. Grazing management of Lotus: use of co-learning to increase adoption of new cultivars. Wool and Sheepmeat Services Program Annual Conference, pp 42-44, October 21 - 23 1997, Orange. Lotus literature 65

AYRES J.F. and BOURKE C. 2003. Lotus – Birdsfoot trefoil. NSW Agriculture Agnote DPI- 413, first edition, January 2003.

AYRES J.F. and BOURKE C. 2003. Lotus – Greater lotus. NSW Agriculture Agnote DPI-412, first edition, January 2003.

AYRES J.F. and LLOYD DAVIES H. 1998. “Lotus use and agronomy”. Grasslands Society of NSW Newsletter, 3, 11-15.

AYRES J.F. and LLOYD DAVIES H. 1999. White clover improvement. Grassland Society of NSW Newsletter, 14, 10-12.

BASSO S.M.S., JACQUES A.V.A. e DALL'AGNOL M. 2002. Alocação da biomassa e correlações morfofisiológicas em leguminosas forrageiras com hábitos de crescimento contrastantes. Scientia Agricola, Piracicaba, 59, 629-634. [Basso S.M.S., Jacques A.V.A. and Dall'agnol M. 2002. Biomass allocation and morphophysiological correlations in forage legumes with contrating growth habits. Scientia Agricola, Piracicaba, 59, 629-634. [Portuguese]] The biomass allocation analysis and the correlation among morphophysiological variables allow greater understanding of the establishment, yield and persistence of perennial species in plant breeding projects, management and ecology of forage plants. To analyze the biomass allocation of forage legumes (Adesmia latifolia, A. punctata, A. tristis, Lotus corniculatus, L. uliginosus), an experiment was carried out under greenhouse conditions for 210 days (4000 degree-days); the plants were cultivated in 1 m2 wooden boxes. Plants were harvested to evaluate the morphological components and to describe their growth habit. The root, stem and leaf biomass allocations were expressed as dry mass percentages. The greatest allocation on leaves occurred for stoloniferous species, A. latifolia (63.5%) and A. punctata (61.4%), which presented the smallest allocation for roots, 10.9 and 14.7%, respectively. Largest stem biomass allocation (39.4%) and roots (24.4%) were found for L. uliginosus, a species with rhizomes. The leaf area index (LAI) was positively correlated with the number of leaves, secondary stems and aerial biomass. A. latifolia and Lotus spp. are extreme examples of the relative importance of morphological components in the formation of LAI and biomass. The accumulation pattern and forage allocation of stoloniferous legumes are mainly characterized by leaf production, pointing out the importance of stolon elongation and rooting, while for A. tristis and Lotus spp., the stem and root fractions had the same importance.

BASSO S.M.S, JACQUES A.V.A, DALL'AGNOL M., RIBOLDI J. e CASTRO S.M.J. 2000. Dinâmica da formação de gemas, folhas e hastes de espécies de Adesmia DC e Lotus L. Revista da Sociedade Brasileira de Zootecnia, Viçosa, 29, 1961-1968. [Basso S.M.S, Jacques A.V.A, Dall'Agnol M., Riboldi J. and Castro S.M.J. 2000. Dynamics of buds, leaves and stems formation of the Adesmia DC. and Lotus L. species. Revista da Sociedade Brasileira de Zootecnia, Viçosa, 29, 1961-1968. [Portuguese]] The genus Adesmia DC. has 17 indigenous species from southern Brazil. The species grow during winter-spring, and their forage potential has recently been studied. This work had as objective to observe the dynamics of bud (B), leaves (L) and stems (S) formation of A. latifolia, A. tristis and A. punctata, using Lotus corniculatus (birdsfoot-trefoil) and L. uliginosus as control species. The assay was conducted under greenhouse conditions, during 210 days (4000 degrees-days); during this period the plants were harvested six times, to evaluate the morphological components. There was a positive response of these variables to the accumulation of degrees day. The densities of B/m2 were, in average, 66 Lotus literature

4679 for Lotus spp., 3000 for A. tristis, 700 for A. punctata and 1200 for A. latifolia. The species of Lotus spp. showed a greater degree of branching (3600 S/m2) and leaf formation, estimated in 15500 L/M2 for birdsfoot-trefoil. In the stoloniferous, A. latifolia and A. punctata, the average percent of stems of the stolon type was approximately 90% and 50%, respectively, at the pre-inflorescence stage. A. tristis and birds foot-trefoil, with an erect growth habit, maintained a mixed population of erect and prostrate stems, while the rhizomatous L. uliginosus, showed 11% of rhizomes at the end of the experimental period. All legumes, but L. uliginosus, reached the flowering stage. A. latifolia maintained about 95 to 99% of its buds at the soil surface level (at the stolon nodes), a desirable characteristic for continuously grazed plants.

BASSO S.M.SCHEFFER., JACQUES A.V.A., DALL'AGNOL M., RIBOLDI J. and CASTRO S M J. 2001. Disponibilidade e valor nutritivo de forragem de leguminosas nativas (Adesmia DC) e exóticas (Lotus L.). Revista Brasileira de Zootecnia, Viçosa, MG, 30, 975-982. [BASSO S.M.SCHEFFER., JACQUES A.V.A., DALL'AGNOL M., RIBOLDI J. and CASTRO S M J. 2001. Availability and nutritive value of the wild (Adesmia DC.) and exotic legumes (Lotus L.). Revista Brasileira de Zootecnia, Viçosa, MG, 30, 975-982. [Portuguese]] The genus Adesmia DC. has 17 species native to Brazil, distributed in the Southern states, whose importance is linked to its adaptation to the soils and climatic conditions of the region, besides being an active winter-growing species (temperate). This work aimed to compare the patterns of dry matter (DM) accumulation and nutritive value of A. latifolia, A. punctata and A. tristis, using Lotus corniculatus (birdsfoot trefoil) and L. uliginosus (big trefoil), as checks. The experiment was carried out in the greenhouse for 210 days (4000 degrees-day). The forage availability (FA) was similar for A. latifolia (276 g DM/m2) and birdsfoot trefoil (275 g DM/m2), as well as for A. tristis (201 g DM/m2) and big trefoil (192 g DM/m2), while A. punctata showed the smallest FD (155 g DM/m2). A. latifolia was characterized by an early FA, due to its fast growth when compared to other species, pointing to its potential utilization during the cold season. In relation to the quality analysis, the crude protein (CP) in A. latifolia leaves was up to 21.6% and the organic matter in vitro digestibility (OMIVD) was up to 72.3%. The highest CP and OMIVD was found in the birdsfoot trefoil leaves, 30.3 and 75.8%, respectively. A. tristis presented a very low OMIVD in the stems, from 34.9 to 44.7%, which could limit its intake by cattle. It is concluded that, among the Adesmia species studied, A. latifolia holds the greatest forage potential and deserves further study.

BASTIANI PEREZ N. 2003. Melhoramento genético de leguminosas de clima temperado - alfafa (Medicago sativa L.) e cornichão (Lotus corniculatus L.) - para aptidão ao pastejo. Tese de Doutorado em Zootecnia, - Plantas Forrageiras, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. (159p.). Orientador: Miguel Dall’Agnol, Julho de 2003. [Bastiani Perez N. 2003. Breeding the temperate legumes – alfalfa (Medicago sativa L.) and birdsfoot trefoil (Lotus corniculatus L.) - to grazing aptitude. Doctoral thesis in Forage Science, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. (159p.) July, 2003. Adviser: Prof. Miguel Dall’Agnol. [Portuguese]] The objective of this work was to assess the genetic variability of different Crioula alfalfa and São Gabriel birdsfoot trefoil populations to grazing tolerance and to select plants more adapted to grazing. The plants were submitted to a heavy and continuous grazing (3-5 cm stubble). At the end of the experimental period there were significant differences (P< 0,05) among alfalfa populations but there were no consistent differences among birdsfoot trefoil treatment, which presented a higher percentage of surviving plants. The alfalfa’s morphological and physiological characteristics were evaluated in order to correlate then with grazing resistance. The results did not show significant differences (P> Lotus literature 67

0,05) among the genotypes in relation to crow contraction, crow area, leaf area, number of stems per plant, type of stem per plant (basilar or axillary), nitrogen content and non-structural carbohydrates of the roots. Greenhouse trials have identified morphological markers on alfalfa and birsfoot trefoil seedlings, which allowed an early identification of genotypes with grazing aptitude. In a greenhouse trial, the effectiveness of the recommend birdsfoot trefoil Rhizobium strains was evaluated in comparison with new strains collected, showing the possibility to isolate new strains. These results allowed the proposition of a new plant functional descriptor named specific buds for characterization of grazing aptitude. Besides that, the term grazing aptitude is proposed to replace grazing tolerance.

BAVAGE A.D., ROBBINS M.P., SKØT L. and WEBB K.J. 1998. Introducing and expressing genes in Legumes. In: Cunningham C. and Porter A. (Eds.) The production of recombinant proteins in plants. Methods in Biotechnology Vol.3 Humana Press. Totowa. N.J. (Dec.1997). pp. 27-38.

BLUMENTHAL M.J., AMPT P., HOCHMAN Z. and AYRES J.F. 1996. Grazing management of lotus: a participatory approach. Proceedings of the 8th Australian Agronomy Conferenc, p. 622.

BLUMENTHAL M.J., AYRES J.F. and HOCHMAN Z. 1997. Grazing management of Lotus in eastern Australia: A participatory approach. Proceedings of the Eighteenth International Grasslands Congress, 29.1-29.2.

BLUMENTHAL M.J., BOWMAN A.M., COLE A., JONES R.M., KELMAN W.M., LAUNDERS T.E. and NOCOL H.I. 1999. Establishment, growth and persistence of greater lotus (Lotus uliginosus) at six sites in eastern Australia. Australian Journal of Experimental Agriculture, 39, 819-827.

BLUMENTHAL M.J. and HARRIS C.A. 1998. Effects of Photoperiod and Temperature on Shoot, Root and Rhizome Growth in Three Lotus uliginosus Schkuhr Populations. Annals of Botany, 81, 55-59.

BLUMENTHAL M.J. and HARRIS C.A. 1993. Maku Lotus soil seedbanks in farmers’ fields. In ‘Proceedings 7th Australian Agronomy Conference’, Adelaide pp 414.

BLUMENTHAL M.J., HOCHMAN Z., AYRES J.F. and NICHOL H. 1995. Lotus Grazing Management for Weaner Production. MRC DAN 082 Protocol.

BLUMENTHAL M.J., KELMAN W.M., HOCHMAN Z. and AYRES J.F. 1995. Improving lotus persistence through breeding and management. Proceedings of the Tenth Annual Conference of the Grassland Society of NSW, p. 86.

BLUMENTHAL M.J., KELMAN W.M., HOCHMAN Z. and AYRES J.F. 1995. Improving lotus persistence through breeding and management. In: Michalk DL (ed) Latest Developments in Pasture Species, Annual Autumn Seminar, 1995, p 16.

BLUMENTHAL M.J., KELMAN W.M., LOLICATO S., HARE M.D. and BOWMAN A.M. 1993. 68 Lotus literature

Agronomy and improvement of Lotus: A review. Alternative Pastures Legumes, pp 74-85.

BLUMENTHAL M.J., KELMAN W.M., LOWTHER W.L., WIDDUP K.H. 1994. The use and management of Lotus in Australian and New Zealand. In ‘The 1st International Lotus Symposium’, 22-24 March 1994, Missouri, USA, pp 125-129.

BLUMENTHAL M.J. and MCGRAW R.L. 1999. Lotus Adaptation, Use, and Management. Trefoil: The science and Technology of Lotus. CSSA Special Publication, 28, Madison, USA.

BLUMENTHAL M.J., O'CONNOR J., AYRES J.F., LANE L.A., HOCHMAN Z. and HINDMARSH J. 1996. Improving lotus persistence through management: site establishment. Proceedings of the Eleventh Annual Conference of the Grassland Society of NSW, pp. 134-135.

BONFANTE P., GENRE A., FACCIO A., MARTINI, I., SCHAUSER, L., STOUGAARD J., WEBB K.J. and PARNISKE M. 2000. The Lotus japonicus LjSym4 gene is required for the successful symbiotic infection of root epidermal cells. Molecular Plant-Microbe Interactions, 13 (10), 1109-1120.

BONFANTE P., GENRE A., FACCIO A., NOVERO M., MARTINI I, SCHAUSER L, STOUGAARD J., WEBB K.J., and PARNIKE M 2000. The Lotus japonicus Sym4 gene is required for the successful infection of root cells by arbuscular mycorrhizal fungi. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. S29.

BOWMAN A.M. 1993. Sharnae – a new Lotus pedunculatus for Australia. Lotus Newsletter, 24, 13-16.

CALLEJAS S., HIDALGO L.G., CAUHÉPÉ M.A. y OTERO M.J. 1999. Efecto del destete precoz sobre la performance reproductiva de vacas de cría multíparas. Revista Argentina de Producción Animal, 19, 323-329. [Callejas S., Hidalgo L.G., Cauhépé M.A. and Otero M.J. 1999. Effect of the early weaning on the reproductive performance of twin breeding cows. Revista Argentina de Producción Animal, 19, 323-329 [Spanish]].

CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1992. Modification of tannin accumulation in Lotus corniculatus using heterologous antisense. SEB 5-10 April, University of Lancaster, UK. J. Expl. Bot. (suppl.) 43:250 (Abs. No. P10.83) p64.

CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1991. Genetic modification of condensed tannin accumulation in transgenic Lotus corniculatus. 4th International Society of Plant Molecular Biology, Tucson, University of Arizona, USA, 6-12th October 1991. Abs. No. 1114.

CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1991. Production and initial analysis of Lotus corniculatus hairy roots transformed with an antisense tannin biosynthetic gene. AFRC Plant Molecular Biology Meeting, University of Reading, UK.

Lotus literature 69

CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1991. Modification of tannin biosynthesis in transgenic root cultures. SEB Meeting, University of Birmingham 7-12 April 1991. P8.69.

CHAMBERLAIN D.A. and WEBB K.J. 1986. A simple and convenient protoplast electrofusion methodology as used in attempts to optimise the production of forage legume heterokaryons. 6th International Congress of Plant Tissue and Cell Culture. University of Minnesota, USA. Abstract no 114.

CITERNE H.L., LUO D., PENNINGTON R.T., COEN E. and CRONK Q.C. 2003. A phylogenomic investigation of CYCLOIDEA-like TCP genes in the Leguminosae. Plant Phisiology, 131, 1042-1053.

COOK R., BARDGETT R.D., EASON W.R., SKØT L. and WEBB K.J. 2001. Biological interactions in grassland soils in relation to productivity. Brazil Abs No.

COOK R., BARDGETT R.D., EASON W.R., SKØT L. and WEBB K.J. 2001. Biological interactions in grassland soils and productivity. In: (Ed.) Grassland Ecosystems: an outlook into the 21st century. Proceedings of the XIX International Grassland Congress, Sao Paulo, Brazil, February 2001, pp.167-168.

COOKE D.E. and WEBB K.J. 1992. Stability of foreign gene expression (CaMV 35S-GUS) in hairy root cultures of Lotus corniculatus under different environmental regimes. SEB University of Lancaster, UK. 5th-10th April 1992. J. Expl. Bot. (suppl.) 43:250 (Abs. No. P10.16) p52.

COOKE D.E. and WEBB K.J. 1994 Considerations when using GUS activity in Lotus corniculatus. LOTUS Newsletter. Ed: PR Beuselinck USDA-Agricultural Research Service. 25:21-24.

COOKE D.E. and WEBB K.J. 1997. Stability of CaMV 35S-gus gene expression in (Bird's foot trefoil) hairy root cultures under different growth conditions. Plant, Cell, Tissue, Organ Culture, 47, 163-168.

COOMBER S, WEBB K.J. and PARNISKE M. 2000. Genes regulating arbuscular mycorrhiza in Lotus japonicus. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. P15.

DALE P.J., FAY M.F., PIKE L.S., WOODCOCK S. and WEBB K.J. 1984. The transfer of new characters to forage legumes. British Grassland Society Occasional Symposium No 16. In: Forage Legumes. Ed: DJ Thomson 66-69.

DALE P.J., WEBB K.J., HALE A., DALTON S.J., PIKE L.S. and WOODCOCK S. 1984. Introducing new characters into forage grasses and legumes. Proceedings of 1st Welsh Research and Development Conference, University College of Wales, Aberystwyth.

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DE LA FUENTE L., QUAGLIOTTO L., BAJSA N., FABIANIO E., ALTIER A. and ARIAS A. 2000. Effect of the introduction of biocontrol Pseudomonas strains on the symbiosis rhizobia - forage legumes. October 29 – November 3, 2000. Proceedings of the Fifth International PGPR Workshop. Córdoba, Argentina. http://www.ag.auburn.edu/argentina/pdfmanuscripts/delafuente2.pdf

DE LA FUENTE L., QUAGLIOTTO L., BAJSA N., FABIANIO E., ALTIER A. and ARIAS A. 2002. Inoculation with Pseudomonas fluorescens biocontrol strains does not affect the symbiosis between rhizobia and forage legumes. Soil Biology and Biochemistry 34:545- 548. Pseudomonas fluorescens strains UP61, UP143 and UP148, isolated from Uruguayan soils, have shown the ability to control soil-borne fungal pathogens that cause damping-off in birdsfoot trefoil. In this communication, we study the effect of these strains on the symbiotic efficiency of rhizobia from commercial inoculants in birdsfoot trefoil, alfalfa and white clover. Shoot dry weights and the rate of nodulation by rhizobia were not modified by the presence of Pseudomonas strains, despite antagonistic activity against rhizobia in vitro. Survival of P. fluorescens UP61 and rhizobia on roots in non-sterile soil were not affected by co-inoculation of the selected forage legumes.

DALTON S.J., JACKSON J.A., CHAMBERLAIN D.A., WEBB K.J. and DALE P.J. 1986. Protoplast culture in forage legumes. Genetic Engineering of Plants and Micro-organisms 65-66.

EASON W.R., ABBERTON M, MICHAELSON-YEATES T., PARNISKE M., TEODOSIO H.R., WEBB K.J. and HOOKER J. 1999. Tools for unravelling genetic control of arbuscular mycorrhizal symbiosis in legumes. 9th International Congress Molecular Plant-Microbe Interactions, Amsterdam, The Netherlands. 25-30th July 1999. Abs. no. 11.14.

FITZGERALD D. and FOGARTY P. 1992. Species for Saline Soils on the Northern Tablelands. In: Proceedings 7th Annual Conference of Grassland Society NSW, pp 91-92.

GIBBS M.J., GATEHOUSE J.A. and WEBB K.J. 1990. Lotus corniculatus: a transformation system with Agrobacterium tumefaciens. 20th Meeting of FEBS, Budapest 19-24 August 1990. Abstr. no. P-Th 484.

GIBBS M.J., GATEHOUSE J.A. and WEBB K.J. 1992. Expression and inheritance patterns of the reporter gene (35S-GUS) in the herbage legume Lotus corniculatus. SEB 5-10 April, University of Lancaster, UK. J. Expl. Bot. (suppl.) 43:250 (Abs. No. P10.61) p60.

GIBBS M. and WEBB K.J. 1989. Lotus corniculatus: A transformation system with Agrobacterium tumefaciens. In: 49th Nottingham Easter School. Genetic Engineering of Crop Plants. 17-21 April 1989.

GORDON A.J., SKØT L., MINCHIN F.R., WEBB K.J., WANG T.L., HEDLEY C.L., CRAIG J. and SMITH A.M. 1997. Down regulation of nodule sucrose synthase by mutation and antisense. Nitrogen Fixation Congress Paris.

GORDON A.J., SKØT L., MINCHIN F.R., WEBB K.J., WANG T.L., HEDLEY C.L., CRAIG J. and Lotus literature 71

SMITH A.M. 1998. Down regulation of nodule sucrose synthase by mutation and antisense. IAPTC Jerusalem 14-19th June 1998 Abstract book p185.

HARRIS C.A., BLUMENTHAL M.J., KELMAN W.M. and MCDONALD L. 1997. Effect of cutting height and cutting interval on rhizome development, herbage production and herbage quality of Lotus pedunculatus cv. Grasslands Maku. Australian Journal of Experimental Agriculture, 37, 631-637.

HARRIS C.A., BLUMENTHAL M.J. and SCOTT J.M. 1992. Survey of use and management of Lotus pedunculatus cv ‘Grasslands Maku’ in Eastern Australia. In: Proceedings 6th Australian Society of Agronomy Conference, Armidale, p 545.

HARRIS C.A., BLUMENTHAL M.J. and SCOTT J.M. 1993. Survey of use and management of Lotus pedunculatus cv. Grasslands Maku in eastern Australia. Australian Journal of Experimental Agriculture, 33, 41-47.

HAYASHI M., MIYAHARA A., SATO S., KATO T., YOSHIKAWA M., TAKETA M., HAYASHI M., PEDROSA A., ONDA R., IMAIZUMI-ANRAKU H., BACHMAIR A., SANDAL N., STOUGAARD J., MUROOKA Y., TABATA S., KAWASAKI S., KAWAGUCHI M. and HARADA K. 2001. Construction of a genetic linkage map of the model legume Lotus japonicus using an intraspecific F2 population. DNA Research, 8, 301-310.

HIDALGO S., CALLEJAS L., CAUHÉPÉ M. y OTERO M.J. 2002. Efecto del destete precoz sobre la ganacia de peso de los terneros y sus madres. Investigación Agraria, España. ITEA, 98A, 347-354. [Hidalgo S., Callejas L., Cauhépé M. and Otero M.J. 2002. Effect of the early weaning on the weight gains of the calves and their mothers. Investigación Agraria, España. ITEA, 98A, 347-354. [Spanish]]

HIDALGO L., ERNIE A. y CAUHÉPÉ M. 1998. Digestibilidad de materia seca y contenido de proteína bruta en especies de pastizal de la Pampa Deprimida, Argentina. Investigación Agraria (España), 1,2,3, 1-13. [Hidalgo L., Ernie A. and Cauhépé M. 1998. Dry matter digestibility and protein content in species of the Depressed Pampas grasslands, Argentina. Investigación Agraria (España), 1,2,3, 1-13. [Spanish]]

HUMPHREYS M.O., HAYWARD M.D., MORRIS P. and WEBB K.J. 1997. Applying biotechnology to grass and forage legume breeding; new solutions, new horizons and new challenges. In: Staszewski Z., Mlyniec W. and Osinski R. (Eds) Ecological Aspects of Breeding Fodder Crops and Amenity Grasses. Proceedings of Eucarpia 20th Fodder Crops and Amenity Grass Section Meeting, Plant Breeding and Acclimatisation Institute, Radzikow, Poland, 7-10 October 1996. pp. 283-292.

HUMPHREYS MO., HAYWARD M.D., MORRIS P. and WEBB K.J. 1997. Applying biotechnology to grass and forage legume breeding new horizons, new challenges and new solutions. Eucarpia Fodder Crops and Amenity Grass Section 20th Meeting. October 7-10 1996. Radzikow, Poland. pp. 157-158. (Astract)

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JØRGENSEN B., SKØT L. and WEBB K.J. 1994. T-DNA insertional mutagenesis and chemical mutagenesis in L. japonicus. CEC AMICA Consortium Book of Abstracts.

JØRGENSEN B., SKØT L. and WEBB K.J. 1995. Initial screening of putative T-DNA tagged Lotus japonicus. SEB St Andrews Meeting, 3-7 April 1995. Abs no. 6.65.

JØRGENSEN B., WEBB K.J. and SKØT L. 1994. Evaluation of Lotus japonicus for a T-DNA tagging programme. VIII International Congress on Plant Tissue and Cell Culture. Firenze, 12-17 June 1994. Abs. No. S1-170.

JØRGENSEN B., WEBB K.J. and SKØT L. 1994. New techniques for identifying nitrogen fixation and nodulation genes using T-DNA tagging in Lotus japonicus. Proceedings of the 1st European Nitrogen Fixation Conference Szeged, Hungary. August 29 - September 2. Eds: GB Kiss and G Endre. Officina Press. P79.

JØRGENSEN B., SKØT L. and WEBB K.J. 1994. A study of the flexibility of the transformation system in Lotus japonicus. Lotus Newsletter. Ed: PR Beuselinck USDA-Agricultural Research Service. 25:25-27.

JUAN V., MONTERROSO L., SACIDO M. and CAUHÉPÉ M. 2000. Postburning legume seeding on a Paspalum quadrifarium community in the Flooding Pampas, Argentina. Journal of Range Management, 53, 300-304.

JUAN V., SACIDO M., CAUHÉPÉ M.A. y BALLETO F.I. 1998. Implantación y establecimiento de Lotus tenuis posquema de Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. Serie Técnica, INIA Uruguay, No. 94, 69-72. ISBN 9974-38-087-1. [Juan V., Sacido M., Cauhépé M.A. and Balleto F.I. 1998. Installation and establishment of Lotus tenuis after burning of Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. Serie Técnica, INIA Uruguay, No. 94, 69-72. ISBN 9974- 38-087-1. [Spanish]]

KELLY R., TEODOSIO R., EASON W., HOOKER J.E. and WEBB K.J. 2001. Physiological markers target key genes in Arbuscular Mycorrhizal clover. ICOM Adelade, Australia July 2001.

KELMAN W.M. and AYRES J.F. 2002. Genetic analysis of seed yield components in birdsfoot trefoil (Lotus corniculatus). In: McComb J.A. (Ed.) Plant Breeding for the 11th Millennium. Proceedings of the 12th Australasia Plant Breeding Conference, Perth WA, 15-20 September 2002, pp 504-506 (Australasian Plant Breeding Assoc Inc).

KELMAN W.M. and AYRES J.F. 2003. Genetic variation for seed yield components in birdsfoot trefoil (Lotus corniculatus L.) Australian Journal of Experimental Agriculture 42, (1) [In press] Lotus literature 73

The persistence of hard-seeded perennial legumes can be facilitated by breeding and management programs that enhance seedling recruitment and maintain plant population density. In northern New South Wales, day-length limits optimal flowering intensity and seed set of the otherwise promising birdsfoot trefoil cultivar, Grasslands Goldie (Lotus corniculatus L.). We studied the feasibility of improving flowering and seed production traits in this cultivar. To measure the extent of genetic variation for seed yield components, half-sib families derived from 45 parent plants collected from a population of cv. Goldie collected from Inverell, New South Wales were grown in the field at Glen Innes NSW and Canberra ACT. Flowering time, flowering intensity and seed yield components (umbels per stem, pods per umbel, pods per stem, seeds per pod and seed mass) were recorded at both sites, while plant weight, height and width were also measured at the Canberra site. There was significant genetic variation among half-sib families and high narrow-sense heritabilities (on a family mean basis) for flowering time (0.73 ± 0.11), flowering intensity (0.66 ± 0.12) and plant height (0.78 ± 0.10). High estimates of narrow-sense heritability for seed yield components indicated that selection advance would be achieved under both sward and spaced plant conditions. There was evidence that selection for prolific flowering in cv. Grasslands Goldie was associated in the progeny populations with an increase in the number of umbels per stem. This component is a readily measured indicator of flowering intensity and should prove useful in developing more prolific and persistent populations from further selection within cv. Goldie and other birdsfoot trefoil populations.

KELMAN W.M. and AYRES J.F. 2003. Genetic variation for seed yield components in birdsfoot trefoil (Lotus corniculatus L.). Australian Journal of Experimental Agriculture, 44, (1) [In press]

KELMAN W.M. and BLUMENTHAL M.J. 1992. Lotus in South-Eastern Australia: Aspects of Forage Quality and Persistence. In: Proceedings 6th Australian Society of Agronomy Conference. The University of New England, Armidale, 10-14 February, pp 460-463.

KELMAN W.M., BLUMENTHAL M.J. and HARRIS C.A. 1992. Evaluation of Lotus pedunculatus and L. corniculatus accessions in south-eastern Australia. Lotus Newsletter, 23, 8-10.

KELMAN W.M., BLUMENTHAL M.J. and HARRIS C.A. 1997. Genetic variation for seasonal herbage yield, growth habit, and condensed tannins in Lotus pedunculatus Cav. and Lotus corniculatus L. Australian Journal of Agricultural Research, 48, 959-968.

KELMAN W.M. and BOWMAN A.M. 1996. Monitoring improvements in seed production and seedling vigour of Greater Lotus (Lotus Pedunculatus Cav.). In: Proceedings 8th Australian Agronomy Conference. The University of Southern Queensland, Toowoomba, 30 January-2 February, pp 341-344.

KIRKHAM K., WEBB K.J., SKØT L. and HUMPHREYS M.O. 1996. Inheritance of transgenes through three generations in an outbreeding legume, Lotus corniculatus. Eucarpia Fodder Crops and Amenity Grass Section 20th Meeting. October 7-10 1996. Radzikow, Poland. p159.

KIRKHAM M., WEBB K.J., SKØT L. and HUMPHREYS M.O. 1997. Inheritance of transgenes in Lotus corniculatus L. Seeds of Progress Conference. 18-19 February 1997. Nottingham, U.84. 74 Lotus literature

Крамина Т.Е. Морфологическая изменчивость Lotus corniculatus L. s. l. в Средней России в связи с систематикой данной группы // Проблемы современной биологии: Тр. 18 науч. конф. мол. ученых биол. фак. МГУ, Москва, 20-24 апр. 1987. Ч. 3 / МГУ. М., 1987. С. 114—116. Деп. в ВИНИТИ 14.09.1987, № 6654-В87. [Kramina T.E. 1987. Morphological variability of Lotus corniculatus L. s. l. in Middle Russia in connection with the systematics of the group // Problems of modern biology: Proc. 18th sci. conf. of young scientists of Biol. Faculty of Moscow State University, Moscow, April 20-24, 1987. Part 3 / MSU. M., 1987. pp. 114-116. Deposited in VINITI 14.09.1987, № 6654-В87 [Russian]].

Крамина Т.Е. Внутривидовая изменчивость Lotus corniculatus L. s. l. на территории Европейской части СССР // Тр. III молодеж. конф. ботаников Ленинграда, Ленинград, апр. 1990. Ч. 1 / Бот. ин-т АН СССР. Л., 1990. С. 116—122. Деп. в ВИНИТИ 14.11.1990, № 5700-В90. [Kramina T.E. 1990. Intraspecies variability of Lotus corniculatus L. s. l. in the European part of the USSR. Proc. III youth conf. of botanists of Leningrad, Leningrad, April 1990. Part 1 / Bot. Inst. of Academy of Science of the USSR. L., 1990. pp. 116-122. Deposited in VINITI 14.11.1990, № 5700-В90 [Russian]].

Крамина Т.Е. Изучение географической изменчивости отдельных морфологических признаков Lotus corniculatus L. s. l. (Leguminosae Juss.) в Европейской России и на сопредельных территориях // Бюл. МОИП. Отд. Биол. 1992. Т. 97, вып. 6. С. 108— 119. [Kramina T.E. 1992. Study of geographical variation of some morphological characters of Lotus corniculatus L. s. l. (Leguminosae Juss.) in European Russia and adjacent territories // Bull. Mosc. Soc. Nat. Biol. Ser., 97, 108-119. [Russian]]. Study of geographical variation pattern of 18 morphological characters of Lotus corniculatus L. s. l. and their relations to variation of macroclimate has been conducted. Polymorphism of the majority of the characters in most populations has been established. Some characters (especially calyx and leaves hair density, length to width ratio for leaflets of a compound leaf, flower size) showed a tendency to clinal variation pattern from north-west to south-east. Mean value correlation between hair density and leaflets size and climatic parameters can suggest their adaptability.

Крамина Т.Е. Изучение морфологической изменчивости в популяциях Lotus corniculatus L. s. l. с применением многомерных методов анализа // Проблемы охраны Дудергофских высот: Тез. IV молодеж. конф. ботаников Санкт-Петербурга (секция сист. и геогр. высших растений). СПб., 1992. С. 32. [Kramina T.E. 1992. Study of morphological variability in populations of Lotus corniculatus L. s. l. by multivariate analysis methods // Problems of conservation of Dudergof heights: Abstr. IV youth conf. of botanists of St.-Petersburg (section of higher plants systematics and geography). SPb., 1992. p. 32 [Russian]].

Крамина Т.Е. Морфологическая и географическая изменчивость Lotus corniculatus L. s. l. (Leguminosae) и проблемы систематики этой группы // Тр. IV молодеж. конф. ботаников Санкт-Петербурга, С.-Петербург, май 1992. Ч. 1 / Бот. ин-т РАН. СПб., 1993. 192 с. Деп. в ВИНИТИ 10.06.1993, № 1621-В93. [Kramina T.E. 1993. Lotus literature 75

Morphological and geographical variability of Lotus corniculatus L. s. l. (Leguminosae) and problems of the systematcs of the group. Proc. IV youth conf. of botanists of St.- Petersburg, St.-Petersburg, May 1992. Part. 1 / Bot. Inst. of RAS. SPb., 1993. Deposited in VINITI 10.06.1993, № 1621-В93 [Russian]].

KRAMINA T.E. 1993. Within- and interpopulation variability of Lotus corniculatus L. species complex in the European Russia and adjacent countries. Proc. IV conf. “Species and its productivity in the distribution area. SPb.: Hydrometeoizdat, 1993. pp. 356-357. // [Материалы IV совещ. “Вид и его продуктивность в ареале”. СПб.: Гидрометеоиздат, 1993. С. 356—357.]

KRAMINA T.E. 1994. Lotus corniculatus L. complex in the European Russia. In: Beuselink P.R. and Roberts C.A. (Eds) Proc. First Intern. Lotus Symposium, 22-24 March 1994. Missouri Botanical Gardens. St. Louis, Missouri, USA / Univ. of Missouri-Columbia, 1994. pp. 25-26.

Крамина Т.Е. Морфологическая изменчивость в популяциях комплекса Lotus corniculatus L. s. l. на юге европейской части России и проблема межвидовых границ в этой группе // IX Московское совещание по филогении растений: Материалы / Под ред. чл.-корр. РАН, проф. В.Н.Тихомирова. - М.: Изд. секции ботаники Моск. о-ва испытателей природы и каф. высших растений биол. ф-та Моск. ун-та, 1996. С. 74—76. [Kramina T.E. 1996. Morphological variation in populations of Lotus corniculatus L. s. l. complex in the south of the European part of Russia and the problem of interspecific limits in this group. In: Tikhomirov V.N. (ed.). Proceedings IXth Moscow conf. of plants phylogeny. M.: 1996. pp. 74-76 [Russian]].

KRAMINA T.E. 1997. Variability of some morphological characters of Lotus corniculatus complex in the European Russia and Ukraine // In: Smets E., Ronse Decraene L.P. and Robbrecht E. (eds) 13th Symposium Morphology, Anatomy and Systematics: Program. and abstr. / Meuse: Nat. Bot. Garden of Belgium, 1997. (Scripta Botanica Belgica; Vol. 15). p. 97.

Крамина Т.Е. Морфология пыльцы некоторых представителей рода Lotus (Fabaceae) // Проблемы ботаники на рубеже XX-XXI веков: Тез. докл., предст. II (X) съезду Рус. бот. о-ва (26-29 мая 1998 г., Санкт-Петербург). Т. 1. СПб.: Изд. БИН РАН, 1998. С. 47—48. [Kramina T.E. 1998. Pollen morphology of several representatives of Lotus (Fabaceae) // Problems of botany in the border of XX-XXI centuries: Abstr. of II (X) Congress of Rus. Bot. Society (May 26-29, 1998, St.-Petersburg). T. 1. SPb.: Bot. Inst. RAS, 1998. pp 47-48. [Russian]].

KRAMINA T.E. 1999. A contribution to the taxonomic revision of the Lotus corniculatus complex (Leguminosae, Loteae) in the European part of the former USSR. Syst. Geogr. Pl., 68, 265-279. The variability in twenty morphological characters was studied in 54 local populations of Lotus corniculatus L. complex in the European part of Russia and adjacent areas. Chromosome numbers 76 Lotus literature were counted for samples taken from 15 locations. A tendency towards a clinal variation has been demonstrated for several characters, especially for leaflets indexes, indumentum density and flower length. Correlations between some morphological characters (leaflets size, indumentum density and others) and climate parameters have been revealed. On the basis of a factor analysis, four species are distinguished in the complex occurring in the study area: L. krylovii Schischk. et Serg. (2n=12), L. tenuis Waldst. et Kit. ex Willd. (2n=12), L. ucrainicus Klok. (2n=12), and L. corniculatus L. (2n=24).

Крамина Т.Е. Значение признаков строения семян для систематики секции Lotus рода Lotus L. (Leguminosae - Papilionoideae - Loteae) // X Московское совещание по филогении растений: Материалы. под ред. проф. Л.И.Лотовой и проф. А.П.Меликяна. - М.: Изд. секц. ботаники Моск. о-ва испытателей природы и каф. морфол. и систематики высш. раст. Моск. гос. ун-та им. М.В.Ломоносова, 1999. С.90—93. [Kramina T.E. 1999. The significance of seed characters in the systematics of the genus Lotus L. sect. Lotus (Leguminosae - Papilionoideae - Loteae) // In: Lotova L.I. and Melikian A.P. (eds.). Proceedings of the X Moscow Conf. on Plants Phylogeny, M.: MSU, pp 90-93. [Russian]].

Крамина Т.Е. Таксономическая ревизия секции Lotus рода Lotus L. (Leguminosae) на территории Европейской России и сопредельных государств. — Моск. гос. ун-т. Дисс. на соиск. уч. степ. канд. биол.наук. М., 1999. [Kramina T.E. 1999. Taxonomic revision of the section Lotus (genus Lotus L., Leguminosae) in the European Russia and adjacent countries. Moscow State Univ., Ph.D. Thesis, Mocsow [Russian]].

Крамина Т.Е. Новый вид рода Lotus L. (Leguminosae) из группы L. corniculatus L. // Бюл. МОИП. Отд. Биол. 2000. Т. 105, вып. 1. С. 35—40. [Kramina T.E. 2000. A new species of Lotus L. (Leguminosae) from L. corniculatus L. group // Bull. MOIP. Sect. Biol., 105, 35-40 [Russian]]. A new species, Lotus stepposus Kramina, distributed in steppe areas of European Russia (and partly in Siberia), Ukraine, Moldova, Romania, Kazakhstan and Turkey is described. The species is characterized by diploid chromosome number 2n=12. Its relations to L. tenuis Waldst. et Kit. ex Willd. (=L. glaber Mill.) and L. corniculatus L. s. str. are discussed.

KRAMINA T.E. 2001. A taxonomic study of the genus Lotus (Papilionoideae, Loteae): generic limits, infrageneric division and the position of some critical species // The Fourth Intern. Legume Conf., 2—6 July 2001, Austral. Nat. Univ., Canberra, Australia: Program and Abstracts. Legume Down Under. pp 46—47.

Крамина Т.Е. Использование корреляций признаков в микросистематике // Междунар. науч. конф. по систематике высших растений, посвященная 70-летию со дня рождения чл.-корр. РАН, проф. В.Н.Тихомирова (Москва, 28-31 января 2002 г.): Тез. докл. — М.: Изд-во Центра охраны дикой природы, 2002. — С.58—59. [Kramina T.E. 2002. The use of feature correlations in microsystematics // International Sci. Conf. on the systematics of higher plants dedicated to 70th anniversary of Associate Academician, Prof. V.N.Tikhomirov’s birthday (Moscow, 28-31 January 2000): Abstr. - M.: BCC-Press. pp 58-59 [Russian]].

Lotus literature 77

Крамина Т.Е., Барыкина Р.П. Сравнительно-анатомический анализ некоторых лядвенцев Японии // Тез. докл. II Междунар. конф. по анат. и морфол. растений. Санкт-Петербург, 14-18 окт. 2002 г. СПб., 2002. С.58-59. [Kramina T.E. and Barykina R.P. 2002. Comparative anatomical analysis of several species of Lotus from Japan // Abstr. II International Conf. Anat. Morphol. of Plants. St.-Petersburg, October 14-18, 2002. SPb. pp.58-59 [Russian]]. Anatomical structure of vegetative organs (i.e. stems, leaves, and roots) in Lotus corniculatus L. var. japonicus Regel, L. corniculatus var. corniculatus, L. krylovii Schischk. et Serg. and discovered in Miyako-jima island in the south of Japan L. sp. nova has been examined. The first two taxa are perennials and the last two are predominantly annuals. Some difference in microstructure of vegetative organs between studied taxa has been revealed. The roots of L. krylovii and L. sp. nova are characterized by thin cork with small number of layers and by the absence of reserve starch that is connected with their annual life cycle. Leaves of L. sp. nova distinguish from those of other studied taxa by the highest number of mesophyll layers and the highest ratio of palisade to spongy mesophyll thickness. L. corniculatus var. japonicus has no additional bundles in stems that are usual for other taxa.

KRAMINA T.E. and SOKOLOFF D.D. 1991. Lotus roudairei Bonnet and taxonomic relationships between African and north American species of the tribe Loteae (Papilionaceae) // Adansonia. Ser. 3, 19, 321-328. A comparative analysis of morphological features in NW African Lotus roudairei, N. American Lotus sect. Simpeteria, and American Lotus sect. Microlotus (gen. Acmispon s. str.) was carried out. According to the data obtained, these three taxa seemed not to form the distinct genus or subgenus Acmispon sensu P. Lassen (1986). A new section Pseudosimpeteria with a single species, L. roudairei, is described within the Old World Lotus subgen. Lotus. The relationships between Old World and New World Loteae are briefly discussed.

KRAMINA T.E. and SOKOLOFF D.D. 1997. Lotus roudairei Bonnet and taxonomic relationships between African and North American species of the tribe Loteae (Papilionaceae). Adansonia, sér. 3., 19, 321-328.

KRAMINA T.E. and SOKOLOFF D.D. 1999. Taxonomic bearing of stylodium tooth in the genus Lotus (Papilionaceae) with special reference to Lotus creticus L. Feddes Repert., 110, 521-527. Stylodium morphology in 62 species of Lotus is studied. In Lotus creticus, a stylodium tooth is detected for the first time. Therefore, L. creticus should be transferred from subgenus Edentolotus into subgenus Pedrosia. L. cytisoides and L. longisiliquosus which were often indicated as scarcely distinguishable from L. creticus, are retained in the subgenus Edentolotus and can be easily separateв from each other if L. creticus is excluded from the subgenus.

Крамина Т.Е., Соколов Д.Д. Kebirita — новый род Leguminosae—Loteae из северо- западной Африки // Бюл. МОИП. Отд. Биол., 2001. Т. 106, вып. 3. С. 58—63. [Kramina T.E. and Sokoloff D.D. 2001. Kebirita - a new genus of Leguminosae— Loteae from North-Western Africa. Bull. Moscow Soc. Natur. Biol. Ser., 106, 58-63. [Russian]]. A new monotypic genus Kebirita Kramina et D.D.Sokoloff (= Lotus sect. Pseudosimpeteria Kramina et D.D.Sokoloff) is decribed from NW Africa. These are perennial herbs without rhizomes with pinnate leaves with 3-6 usually alternate leaflets and glandular stipules. They possess one-flowered umbels on short peduncles. On the peduncle there are two opposite small scaly leaves, one of them is the flower 78 Lotus literature covering leave. There are no leaves with normally developed blade on the peduncle. Ovules micropylae alternates. Pollen grains with 3 apertures. The single species of the genus, Kebirita roudairei (Bonnet) Kranima et D.D.Sokoloff (= Lotus roudairei Bonnet), is distributed in arid areas of Algeria, Tunisia, Morocco, Mauritania and West Sahara.

KRAMINA T.E. and SOKOLOFF D.D. 2003. On section Erythrolotus of the genus Lotus and related taxa. Bull. Moscow Soc. Natur. Biol. Ser., 108, N 5. [Russian](in press). Sect. Erythrolotus is accepted in narrow sense, comprising only L. conibricensis Brot., the species which was selected as a lectotype of the section. L. conimbricensis share with members of sect. Lotus mainly Mediterranean distribution and base chromosome number x=6. L. conimbricensis is characterised by long and narrow, incurved and tardily dehiscent fruit. The majority of species traditionally included in section Erythrolotus is placed now in the section Heinekenia Webb et Berth. A new section, Chamaelotus is described to include three annual species with shortened peduncles and small pink or red flowers. A new combination at sectional level is made, namely sect. Benedictella (Maire) Kramina et D.D. Sokoloff. The section Benedictella is monotypic and includes L. benoistii (Maire) Lassen from Morocco.

Крамина Т.Е., Соколов Д.Д. Дисткретность и континуальность таксонов и их диагностических признаков в систематике рода Lotus L. (Leguminosae) // XI Международное совещание по филогении растений. Тезисы докладов (Москва, 28- 31 января 2003 г.) / Под ред. В.С.Новикова, А.К,Тимонина и А.В.Щербакова. — М.: Изд-во Центра охраны дикой природы, 2003. С. 54-55. [Kramina T.E. and Sokoloff D.D. 2003. Classification and evolution of the genus Lotus L. (Leguminosae) // XI International plant phylogeny symposium. Abstracts (Moscow, 28-31 January 2003) / V.S.Novikov, A.K.Timonin, A.V.Shcherbacov (eds.). M.: BCC-Press, 2003. p. 54-55 [Russian]].

Крамина Т.Е., Тихомиров В.Н. Внутрипопуляционная изменчивость отдельных морфологических признаков Lotus corniculatus L. s. l. (Leguminosae) на территории европейской части СССР // Бюл. МОИП. Отд. Биол. 1991. Т. 96, вып. 6. С. 117—126. [Kramina T.E. and Tikhomirov V.N. 1991. Within populational variability of some morphological characters of Lotus corniculatus L. s. l. (Leguminosae) in the European part of the USSR. Bull. Mosc. Soc. Nat. Biol. Ser., 96, 117-126. [Russian]]. The study of variability limits and estimation of diagnostic values of 18 morphological characters, being usually referred to in determination of taxa within the Lotus corniculatus L. s. l. complex from the European part of the USSR have been carried out, basing on the data of samples from 22 local populations. Each character was shown to be of overlapping variability within the adjacent populations, representing the argument against the independence of the studied races (L. ucrainicus Klok., L. ruprechtii Min., L. balticus Min., L. zhegulensis Klok., L. callunetorum (Juxip.) Min., and L. corniculatus L. s. str.). The received data require further examination with the help of multidimentional methods.

LATERRA P. 1997. Post-burn recovery in the flooding Pampa: impact of an invasive legume. J. Range Manage, 50, 274-277.

LATERRA P. and BAZZALO M.E. 1999. Seed to seed allelopathic effects between two invaders of burned grasslands in the Flooding Pampa. Weed Research, 39, 297-308. Lotus literature 79

The establishment of Lotus tenuis Waldst et Kit. (narrowleaf birdsfoot trefoil) can interfere with colonisation by Carduus acanthoides L. (musk thistle) during the early postburn recovery of Flooding Pampa grasslands. The purpose of this research was to determine the potential role of L. tenuis seeds as source of allelopathic compounds involved in that interaction. Imbibed seeds of L. tenuis and aqueous leachates from them were bioassayed for their ability to inhibit germination and seedling growth of C. acanthoides, both on sterilised filter paper and on pasteurized soil as substrata. Germination or emergence of C. acanthoides were inhibited and root length was reduced on filter paper or soil, by both the presence of L. tenuis seeds and by their leachate, at densities of L. tenuis near the maximum values observed in the field. Germination and seedling growth of C. acanthoides were less affected by the presence of L. tenuis seeds than by the addition of their leachate, and the presence of L. tenuis seeds or their leachate showed stronger effects on emergence of C. acanthoides from soil than on its germination on filter paper. Methods applied for leachate sterilization, ultrafiltration or autoclaving, did not modify C. acanthoides responses. Neither the germination rate nor the root length of C. acanthoides seedlings were affected by solutions of polyethylene glycol with similar osmolarity to the leachates. We conclude that the release of inhibitory substances onto filter paper and into pot soil from imbibed L. tenuis seeds would be the mechanism responsible for the observed effects.

LATERRA P. and SOLBRIG O.T. 2001. Dispersal strategies, spatial heterogeneity and colonization success in fire-managed grasslands. Ecological Modelling, 139, 17-29. Interactions between fire regime, dispersal strategies and patch structure were examined as key issues for the management of floristic composition of grasslands, through a model that simulates the population dynamics of two competing fire-cued and non-sprouting species. The model describes a heterogeneous environment composed by several patches of grassland, only related by seed dispersal. The last burn date at each patch determines the accumulation level of fuel-biomass provided by a third, dominant species, which in turn controls for the exclusion rate of both colonizer species. The population dynamics of both species was approached following density-dependent models and parameterized for two opposite dispersal strategies: low spatial and high temporal dispersion of seeds (type 1), high spatial and low temporal dispersion of seeds (type 2). Only under the most variable scenarios (when non-synchronous and irregular fire regimes were combined with a proportion of patches (p) with initially depleted seed banks) did the relative success of dispersal strategies vary with the length of the fire-free period. Irrespective of p, smaller interval lengths favored the postburn density of the strategy 1. Strategy 2 was favored over strategy 1 when the fire-free interval increased, such difference being maximum for intermediate p values. These general tendencies agree with those observed from a reference system: the Flooding Pampa grasslands dominated by Paspalum quadrifarium where short no-fire intervals promote the postburn abundance of a type 1 species (Lotus tenuis) over two type 2 species (Carduus acanthoides and Cirsium vulgare) while for long fire-free intervals the opposite is true. These results suggest that frequency, time since last burn, and burning synchrony are useful components of a fire regime to take advantage of variation in dispersal strategies.

LATERRA P., VIGNOLIO O.R., HIDALGO L.G., FERNÁNDEZ O.N., CAUHÉPÉ M.A. y MACEIRA N.O. 1998. Dinámica de pajonales de paja colorada (Paspalum spp) manejados con fuego y pastoreo en la pampa deprimida argentina. Ecotrópicos, 11, 11-149. [Laterra P., Vignolio O.R., Hidalgo L.G., Fernández O.N., Cauhépé M.A. and Maceira N.O. 1998. Dynamics of grasslands of red straw (Paspalum spp) managed with fire and grazing in the Argentinean depressed pampas. Ecotrópicos, 11, 11-149. [Spanish]]

LATERRA P., VIGNOLIO O.R., LINARES P., GIAQUINTA A. and MACEIRA N.O. 2003. Cumulative effects of fire on the structure and function of a tussock pampa grassland. Journal of Vegetation Science, 14, 43-54. 80 Lotus literature

In order to test for cumulative effects of fire on Paspalum quadrifarium-dominated grasslands (“pajonal”), we analyzed the impact of single and repeated fires on the community structure and post- fire recovery of canopy after a final, simultaneous fire event. Nine plots were defined within a homogeneous pajonal stand, and treatments of low (LF), medium (MF) and high frequency (HF) of fire were defined by the application of one, two or four cold-season burnings, respectively, along a six-year period. Both burned and unburned plots were exposed to grazing by cattle during the summer following the first and the third years of that period. High cattle preference for burned sites conditioned fire temperature and vegetation responses to the following burning events. Cumulative effects between successive burning events were observed on the cover of basal area of the dominant and other sprouter species, the cover and thickness of the litter layer, the seed bank size of the principal recruiter species, and the floristic composition. While light interception by the canopy was positively related to fire frequency during the early growth season, further growth of P. quadrifarium determined a greater light interception in LF than in MF and HF. These patterns of light interception were associated with a faster occupation of the between-tussock areas by opportunistic species in plots subjected to frequent fires (HF and MF) than in plots with low fire frequency (LF), and a more lasting regrowth of P. quadrifarium in the LF plot than in the HF ones. High fire frequencies reduced the dominance of P. quadrifarium. Percent of species classified as subordinated graminoids or forbs did not vary among treatments. However, the abundance of different forb species was differentially favored by contrasting frequencies of fire, describing some coarse relationships between their specific responses and their dispersal strategies.

LEPEK V.C., D’ANTUONO A.L., TOMATIS P.E., UGALDE J.E., GIAMBIAGI S., and UGALDE R.A. 2002. Analysis of Mesorhizobium loti glycogen operon: effect of phosphoglucomutase (pgm) and glycogen synthase (glgA) null mutants on nodulation of Lotus tenuis. Molecular Plant-Microbe Interactions 15, 368-375.

LEWIS M., SKØT L. and WEBB K.J. 1993. The manipulation of soil microbe populations via transgenic plants. Molecular Biology and Evolution. UCW-IGER Aberystwyth UK 4th-6th January 1993.

MARTINS E.S. and SOKOLOFF D.D. 2003. Lotus robsonii E.S. Martins & D.D. Sokoloff, sp. nov. In: Flora Zambesiaca. Vol. 3. Pt. 7. Kew: Royal Botanic Gardens, pp. 3–4. A new species of Lotus is described from Malawi. It differs from related species by leaves with 3, not 5 leaflets.

MCLAUGHLIN B.D. and CLARKE J.B. 1989. Lotus for pasture and seed production. NSW Agriculture Agfact P2.5.30, first edition.

MONTERROSO L., JUAN V., SACIDO M. y CAUHÉPÉ M.A. 1998. Incidencia y severidad de patógenos en Lotus tenuis durante su implantación y establecimiento en pajonales de paja colorada (Paspalum quadrifarium). Revista Latinoamericana de Fitopatología, 33, 224- 227. [Monterroso L., Juan V., Sacido M. and Cauhépé M.A. 1998. Disease incidence and severity in Lotus tenuis during their installation and establishment in grasslands of red straw (Paspalum quadrifarium). Revista Latinoamericana de Fitopatología, 33, 224-227. [Spanish]]

MORRIS, P., CARRON T.R., ROBBINS M.P. and WEBB K.J. 1993. Distribution of condensed Lotus literature 81

tannins in flowering plants of Lotus corniculatus var japonicus and tannin accumulation by transformed root cultures. LOTUS Newsletter.

MORRIS P., ROBBINS M.P. and WEBB K.J. 1994. Elicitation of phytoalexins in hairy root culutres of Lotus cornciculatus, Trifolium repens and Cicer arietinum. SEB Univeristy College of Swansea, UK. 11-15 April 1994. [P3.1]

MORRIS P., SKØT L., WEBB K.J., JØRGENSEN B. and ROBBINS M.P. 1998. Application of Biotechnology to Lotus breeding. In: Trefoil: Beuselinck P., Nelson J. and Hoveland C. (eds) The Science and Technology of Lotus. American Society of Agronomy and Crop Science. (ASA-CSSA-SSSA).

MORRIS P., WEBB K.J. and ROBBINS M.P. 1990. Phenyl-propanoid metabolism in Agrobacterium rhizogenes transformed cultures of herbage legumes. VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam 24-29 June 1990. Abstr. no. C5-40

MORRIS P., WEBB K.J., ROBBINS M.P. and SKØT L. 1994. Applications of tissue culture, molecular biology and genetic manipulation in Lotus research. Proceedings First International Lotus Symposium, 22-24 March 1994, St Louis, Missouri, USA. Ed: P Beuselinck. pp. 103-108.

MORRIS P., WEBB K.J., ROBBINS M.P., SKØT L. and JØRGENSEN B. 1999. Application of Biotechnology to Lotus Breeding. In: Beuselinck P., Nelson J. and Hoveland C. (eds) Trefoil: The Science and Technology of Lotus. CSSA Special Publication 28. pp. 199-228. Crop Science Society of America and American Society of Agronomy (ASA-CSSA), Madison, USA. ISBN 089118550X.

NICHOLSON M. [WEBB K.J., SKØT L., JØRGENSEN B. and MIZEN S.] 1998. GUS activity. Microscopical detection of gene activity in legume roots is one step closer to cloning nitrogen fixation genes. Microscopy and Imaging News 150 April p5.

NICHOLSON M.M., JØRGENSEN B., MIZEN S., SKØT L. and WEBB K.J. 1996. Tagging nodulation and nitrogen fixation genes in Lotus japonicus. Molecular Plant-Microbe Interactions. Knoxville, Tennessee, USA. 14-19 July 1996. Abs. No. 31.

NICHOLSON M.M., JØRGENSEN B., MIZEN S., SKØT L. and WEBB K.J. 1996. Tagging nodulation and nitrogen fixation genes in Lotus japonicus. SEB, Dublin, Eire. 22-26 July 1996. Abstract no P12.

NICHOLSON M.M., JØRGENSEN B., MIZEN S., SKØT L. and WEBB K.J. 1997. Tagging nodulation and nitrogen fixation genes in Lotus japonicus. PMBII Final Meeting, 12-14th February 1997. Norwich, U.K. Abs. No. 5.

NICHOLSON M., WEBB K.J. and SKØT L. 1996. Nitrogen fixation genes - falling into the promoter trap. Conference of Plant Science Wales 9-10 January 1996. IGER, Aberystwyth, UK. 82 Lotus literature

NISBET G.S. and WEBB K.J. 1990. Transformation in legumes. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry Vol 10. Legumes and Oilseed Crops I. Springer- Verlag, Berlin. pp. 38-48.

NOVERO M., FACCIO A., GENRE A., STOUGAARD J., WEBB K.J., MULDER L., PARNISKE M. and BONFANTE P. 2002. Dual requirement of the LjSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots. New Phytologist, 154 (3), 741-749.

OLMOS F. 2001. Mejoramiento de pasturas con lotus en la región noreste. INIA Uruguay, Serie Técnica INIA Tacuarembó, No. 124, 48 p. [Olmos F. 2001. Improvement of natural grasslands with lotus in the northeast region. INIA Uruguay, Serie Técnica INIA Tacuarembó, No. 124, 48 p. [Spanish]]. In the period 1992-1996 four experiments were carried out to identify forage legumes (Lotus, Trifolium and Ornithopus) adapted to grow in the natural pastures of the northeast region of the country. Lotus was the genera better adapted when oversown in the natural pastures. The soils generally have a low P content compared to the legume requirements. In the fourth growing season 5 and 75 % of the -1 improved pasture was Lotus corniculatus L. when 0 and 80 kg of P2O5 ha were applied each year. -1 LAI of the legume increased from 1.5 to 5.0 as the P2O5 increased from 0 to 120 kg ha . There was a strong correlation between seed production and seedling recruitment in the following season. In the first growing season 1 plant dm-2 was recorded, while in the third recording period 3 and 6.4 plants dm- 2 -1 were recorded when 0 and 120 kg ha of P2O5 were applied respectively. Based on plant survival and fecundity a model using a Leslie matrix was developed. The model emphasize the need of seedling recruiment every year to keep the introduced population in natural pasture.

OREA A., PAJUELO P., PAJUELO E., MÁRQUEZ A.J. and ROMERO J.M. 2001. Characterisation and expression studies of a root cDNA encoding for ferredoxin-nitrite redcutase from Lotus japonicus. Physiologia Plantarum, 113, 193-202.

OREA A., PAJUELO P., PAJUELO E., QUIDIELLO C., ROMERO J.M. and MÁRQUEZ A.J. 2002. Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase. Physiologia Plantarum, 115, 352-361.

ORTEGA E. and LATERRA P. 2003. Fire-cued colonization of a Flooding Pampa grassland by thistle species: remnant litter and interference effects. Applied Vegetation Science, 6, 35- 44. Winter and Spring burnings constitute a frequent management tool of native grasslands dominated by the bunch grass Paspalum quadrifarium (hereafter "pajonal stands") in the Flooding Pampa of Argentina. In addition to increasing the primary productivity and the nutritious quality of the regrowth, this practice favours the establishment of opportunistic species, especially Lotus tenuis ("lotus"), Cirsium vulgare and Carduus acanthoides ("thistles"). The aims of the present study were to assess the effects of burning and those of the remnant litter on L. tenuis and thistle recruitment, as well as the effects of L. tenuis density on the emergence, survival and flowering of thistles. Two field experiments were carried out. In the first, a completely randomised factorial design with occurrence of L. tenuis and fire was used and, in the second, a completely randomised design with L. tenuis seed density as the only factor. Lotus tenuis and thistle recruitment within the pajonal stand was absolutely dependent on Lotus literature 83 fire. While the presence of L. tenuis and thistles at small spatial scale depended on nearly complete combustion of litter, early presence of thistle seedlings in denuded microsites showed a negative association with the proximity of L. tenuis seedlings. By the end of the first post-fire growing season, the survivorship of established thistles was linearly reduced with the sowing density of L. tenuis. Keeping a significant litter coverage as well as favouring the presence of a high density of L. tenuis in the pre-fire seed bank would constitute management measures aimed to preclude thistle invasion of burned pajonal stands, thus reducing the dependency on chemical control.

ORTEGA E., VERGARA P., VIGNOLIO O. y LATERRA P. 2000. Efectos de la densidad de Lotus tenuis sobre la emergencia, supervivencia y cobertura de Cirsium vulgare. Ecología Austral 10: 143-149. [Ortega E., Vergara P., Vignolio O. and Laterra P. 2000. Effects of the density of Lotus tenuis on the establishment, survival and covering of Cirsium vulgare. Ecología Austral 10: 143-149. [Spanish]] After burning for raising the stocking rate, the Paspalum quadrifarium-dominated grasslands of the Flooding Pampa become invaded by a forage legume, Lotus tenuis, and /or by Cirsium vulgare between other weed species. Aiming to compare the relative impact of L. tenuis on pre and post-emergence stages of C. vulgare, the emergence, survivorship and coverage of C. vulgare, were evaluated in response to four sowing densities of L. tenuis (0,0; 0,25; 0,5 and 1,0 seeds / cm²) and two spatial patterns of C. vulgare (15 uniformly distributed seeds within a circle of 2,5 cm diameter and 15 evenly spaced seeds on a circle of 14 cm diameter), by using a factorial design under greenhouse conditions. The emergence of C. vulgare lineally declined with increasing densities of L. tenuis, independently of its spatial patterns of sowing. While the emergence of C. vulgare was reduced up to 40% by the presence of L. tenuis, the effects of this species on the seedling mortality of the emerged seedlings reached up to 270% of increment. In contrast with the emergence , which was mostly affected by the maximum densities of L. tenuis, the final coverage of C. vulgare was similarly reduced for the presence of L. tenuis irrespective of its density. This results confirm the occurrence of pre-emergent effects of L. tenuis seeds on C. acanthoides seeds, as previously detected under laboratory conditions, even under potentially adverse conditions for the activity of allelopathic compounds.

PACIOS-BRAS C., SCHLAMAN H.R.M., BOOT K., ADMIRAAL P., MATEOS LANGERAK J., STOUGAARD J. and SPAINK H.P. 2003. Auxin distribution in Lotus japonicus during root nodule development. Plant Molecular Biology, 52, 1169-1180. http://www.kluweronline.com/issn/0167-4412/current For this work, Lotus japonicus transgenic plants were constructed expressing a fusion reporter gene consisting of the genes β-glucuronidase (gus) and green fluorescent protein (gfp) under control of the soybean auxin-responsive promoter GH3. These plants expressed GUS and GFP in the vascular bundle of shoots, roots and leafs. Root sections showed that in mature parts of the roots GUS is mainly expressed in phloem and vascular parenchyma of the vascular cylinder. By detecting GUS activity, we describe the auxin distribution pattern in the root of the determinate nodulating legume L. japonicus during the development of nodulation and also after inoculation with purified Nod factors, N- naphthylphthalamic acid (NPA) and indoleacetic acid (IAA). Differently than white clover, which forms indeterminate nodules, L. japonicus presented a strong GUS activity at the dividing outer cortical cells during the first nodule cell divisions. This suggests different auxin distribution pattern between the determinate and indeterminate nodulating legumes that may be responsible of the differences in nodule development between these groups. By measuring of the GFP fluorescence expressed 21 days after treatment with Nod factors or bacteria we were able to quantify the differences in GH3 expression levels in single living roots. In order to correlate these data with auxin transport capacity we measured the auxin transport levels by a previously described radioactive method. At 48 h after inoculation with Nod factors, auxin transport showed to be increased in the middle root segment. 84 Lotus literature

The results obtained indicate that L. japonicus transformed lines expressing the GFP and GUS reporters under the control of the GH3 promoter are suitable for the study of auxin distribution in this legume.

PAJUELO P., PAJUELO E., OREA A., ROMERO J.M. and MÁRQUEZ J.A. 2002. Influence of plant age and growth conditions on nitrate assimilation in roots of Lotus japonicus plants. Functional Plant Biology, 29, 485-494

PARNISKE M., COOMBER S., KISTNER C., MULDER L., PITZCHKE A., STOUGAARD J., SZCYGLOWSKI K., WEBB K.J. and STRACKE S. 2000. Plant genetics of symbiosis. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. S27.

PARNISKE M., COOMBER S., KOYAMA M., WEBB K.J., SZCZYGLOWSKI K., SANDAL N. and STOUGAARD J. 1999. Mycorrhiza mutants of Lotus japonicus. 9th International Congress Molecular Plant-Microbe Interactions, Amsterdam, The Netherlands. 25-30th July 1999. Abs. no. 11.10.

PARNISKE M., WEBB K.J., MARTINI I., GENRE A.and BONFANTE P. 1998. Analysis of Gigaspora and Glomus infection sites in symbiotic mutants of Lotus japonicus. International Congress of Mycorrhiza. Upsala, Sweden. Abs no.231.

PARNISKE M., WEBB K.J., MARTINI I., GENRE A., BONFANTE P., SZCYGLOWSKI K., DE BRUIJN F., SCHAUSER L., SANDAL N. and STOUGAARD J. 1998. Mycorrhiza mutants of Lotus japonicus. First International Lotus japonicus Workshop. 20-22nd November 1998, University of Aarhus, Denmark.

PARNISKE M., WULFF B., HUGHES D. and WEBB K.J. 1996. Mycorrhizal mutants of Lotus japonicus. Emerging Model Legume Systems: Tools and Recent Advances. Knoxville, Tennessee July 12-14, 1996. Abs. no. 15.

PARUELO J.M., OESTERHELD M., DI BELLA C.M., ARZADÚN M., LAFONTAINE J., CAUHÉPÉ M. and REBELLA C.M. 2000. Estimation of primary productivity of subhumid rangelands from remote sensing data. Appl. Vegetation Science, 3, 188-195.

PEDROSA A., SANDAL N., STOUGAARD J., SCHWEIZER D.and BACHMAIR A. 2002. Chromosomal map of the model legume Lotus japonicus. Genetics, 161, 1661-1672.

PÉREZ C., DE LA FUENTE L., ARIAS A. y ALTIER A. 2001. Uso de Pseudomonas fluorescentes nativas para el control de enfermedades de implantación en Lotus corniculatus L. Agrociencia, V, 41-47. [Pérez C., De La Fuente L., Arias A. and Altier A. 2001. Use of native fluorescent Pseudomonas for controlling seedling diseases of Lotus corniculatus L. Agrociencia, V, 41-47. [Spanish]] Seedling diseases caused by Pythium spp. are one of the main constraints for pasture productivity and persistence. During 1996, 1997 and 1998, experiments were conducted under field conditions to Lotus literature 85 evaluate the ability of three strains of native fluorescent Pseudomonas (UP61, UP143, UP148) that produce HCN, siderophores and antibiotics to suppress seedling diseases on Lotus corniculatus. The experimental design was a Randomized Complete Block with five replications. One hundred viable seeds were sown per plot, previously treated as follows: Mesorhizobium loti B816 with each one of the strains of Pseudomonas; M. loti B816 without Pseudomonas; M. loti B816 + metalaxyl (fungicide) sprayed on the soil. Favorable conditions for the development of damping-off only occurred during 1996 (rainfall and low temperatures). That year, despite differences were not statistically significant, the treatments inoculated with Pseudomonas had higher establishment percentage and higher dry matter production per plot than the control without Pseudomonas. In 1997 and 1998, the inoculation with Pseudomonas did not increase the number of established plants but induced an increase on dry matter production per plot suggesting the possible occurrence of an effect on plant growth promotion. The observed trends indicate that the experiments should be repeated in order to validate the practical implementation of this management strategy, under the various soil and environmental conditions and microbial community structures found in Uruguay.

PEREZ N.B., DALL'AGNOL M., DALBOSCO M., BANGEL E.V., MAEYER J. e OLIVEIRA A.M.R. 2003. Caracterização e seleção de estirpes de Rizobium spp em plantas de cornichão. In: Reuniao Anual da SBZ, 2003, Santa Maria. Anais da SBZ. Editora SBZ- CD ROM, 40, p.1-5. [Perez N.B., Dall'Agnol M., Dalbosco M., Bangel E.V., Maeyer J. and Oliveira A.M.R. 2003. Characterization and selection of birdsfoot trefoil Rhizobium isolates. In: Reuniao Annual da SBZ, 2003, Santa Maria. Anais da SBZ. Editora SBZ- CD ROM, 40, p.1-5. [Portuguese]] The objective of this work was to select rhizobium isolates for birsdfoot trefoil, ‘Lotus corniculatus’, by collecting nodules on plants on four cities of Rio Grande do Sul. Through a collaborative work between MIRCEN-FEPAGRO and UFRGS it was possible to characterize the collected material by using immuno-specific serum. The isolated characterization has revealed the existence of antigenic variability. After the characterization the isolated were tested in a complete block design experiment in the greenhouse with three replicates. The results indicated that there is variability for the isolates and that it is possible to select isolated more efficient than the ones that are recommended.

PERRY J.A., WANG T.L., WELHAM T.J., GARDNER S., PIKE J.M., YOSHIDA S. and PARNISKE M. 2003. A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. Plant Physiology, 131, 866-871.

PIPPOLO L. 1998. Incidencia de la avispita Bruchophagus platypterus Walker (Hymenoptera, Eurytomidae) en la producción de semilla de Lotus corniculatus L. en diferentes fechas de floración. Montevideo, Facultad de Agronomía. Tesis Ingeniero Agrónomo. 39 p. Orientador: Alzugaray R. [Pippolo L. 1998. Incidence of the seed chalcid Bruchophagus platypterus Walker (Hymenoptera, Eurytomidae) on seed production of Lotus corniculatus L. in different flowering dates. Montevideo, Faculty of Agronomy. Bachelor Degree Thesis. 39 p. Adviser: Alzugaray R. [Spanish]] The production of seeds of Lotus corniculatus is a very important item within the Uruguayan farming system, especially in the southern-coast region. The loss of seeds caused by the "leguminous seed wasp" (Bruchophagus platypterus Walker) has been observed and mentioned in several publications but no quantification of these losses has been made. With the purpose of doing so and in an attempt to evaluate the incidence of the damage produced at different dates during the flowering stage, an experiment on a land-parcel basis was carried out during the '88-'89 crop. The experiment in question 86 Lotus literature was carried out at La Estanzuela, in Colonia. During November and December, open flowers were remarked the same day they opened and the pods were separately harvested, and manually thrashed. Furthermore, the count of -sound seeds and pods damaged by the wasps was carried out the different dates. The count of wasps and parasites was also made on each flowering date. The damage caused was expressed as the coefficient between the number of damaged seeds and the number of sound seeds, and the relative incidence of parasitoids was expressed as the coefficient between the number of parasitoids and the number of wasps. The highest damage levels were recorded during the earlier dates of flowering (39.6% and 37.7% on 9.11.88 and 16.11.88, respectively) while the lower levels were observed at the end of the period under analysis (4.46% and 5.01% on 6.12.88 and 14.12.88, respectively). In accordance with the results obtained, it was possible to corroborate the existence of Bruchophagus platypterus in Lotus corniculatus, as well as the direct damage it causes on the yield of seeds. It was furthermore proved that the number of wasps is regulated by parasitoids.

QUAGLIOTTO L., AZZIZ G., BAJSA N., VAZ P., PÉREZ C., DUCAMP F., ALTIER N. and ARIAS A. 2003. Pseudomonas strains isolated from Lotus corniculatus rhizosphere as biocontrol agents in alfalfa. Proceedings of the Sixth International PGPR Workshop. October 5-10, 2003. Calicut, India.

RISPAIL N., MORRIS P., NASH R. and WEBB K.J. 2001. Characterisation of metabolic interactions between Lotus japonicus and its symbionts (Rhizobium and arbuscular mycorrhiza). Molecular Genetics of Model Legumes: Impact for legume Biology and Breeding 15-19th Sept 2001 101.

RISPAIL N., MORRIS P., NASH R. and WEBB K.J. 2002. Influence of infection with AM fungi or Rhizobium on root flavonoid content of Lotus japonicus. Ist International Conference on Legume Genomics and Genetics. Minneapolis USA. June 2002. PM14

ROBBINS M.P., CARRON T.R. and WEBB K.J. 1993. Detecting transgenes in Lotus corniculatus using the polymerase chain reaction. LOTUS Newsletter.

ROBBINS M.P., CARRON T.R. and WEBB K.J. 1994. Detecting transgenes in Lotus corniculatus using the polymerase chain reaction. Lotus Newsletter.

ROBBINS M.P., DAVIES T.E. and WEBB K.J. 2000. Lotus japonicus as a model for studying condensed tannin pathways. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. P41.

ROBERTS K., COUTTS J., AYRES J.F. and BILSTON L. 2002. Co-learning in the development of lotus pasture technology in Australia. Australian Journal of Experimental Agriculture, 42, 527-533. A five-year project was undertaken in New South Wales, Australia to develop and implement lotus pasture technology (Greater lotus, Lotus uliginosus; birdsfoot trefoil, Lotus corniculatus). The project was modelled on a core experiment/co-learning paradigm. A core experiment investigated issues of species adaptation and grazing management, and a co-learning phase aimed at promoting simultaneous, improvement and adoption of lotus technology. Most participants felt that involvement with co- learning gave them increased technical knowledge of lotus. Increased knowledge facilitated changes that included farming practice changes (such as establishing lotus pastures or modifications to grazing Lotus literature 87 management), or changing the approach to learning and problem solving towards a co-learning mode. The strengths of the co-learning model were that it was flexible, farmers and industry sponsors worked together, and it increased the effectiveness of the project. The weaknesses were that those groups with informal structures found it difficult to function as a team, and the time limits imposed by the project limited the learning experience.

RHODES I. and WEBB K.J. (1993) Improvement of white clover. CAB International. Outlook on Agriculture. pp. 189-194.

SACIDO M. y CAUHÉPÉ M.A. 1998. Calidad de los rebrotes posquema de pajonales de Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. INIA Uruguay, Serie Técnica, No. 94, 73-78. ISBN 9974-38-087-1. [Sacido M. and Cauhépé M.A. 1998. Quality of the regrowth after burning grasslands with Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. INIA Uruguay, Serie Técnica, No. 94, 73-78. ISBN 9974-38-087-1. [Spanish]]

SANDAL N., KRUSELL L., RADUTOIU S., OLBRYT M., PEDROSA A., STRACKE S., PARNISKE M., BACHMAIR A., KETELSEN T. and STOUGAARD J. 2002. An AFLP based genetic linkage map of the model legume Lotus japonicus developed from an interspecific F2 mapping population. Genetics, 161, 1673-1683.

SCHEFFER-BASSO S.M., VENDRUSCOLO M.C., BARÉA K., et al. 2002. Comportamento de leguminosas (Adesmia, Lotus, Trifolium) em mistura com festuca. R. Bras. Zootec., 31, 2197-2203. [Scheffer-Basso S.M., Vendruscolo M.C., Baréa K., et al. 2002. Response of legumes (Adesmia, Lotus, Trifolium) in mixture with tall fescue. R. Bras. Zootec., 31, 2197-2203. [Portuguese]] Overseeding temperate legumes is one of the most practices to increase the quantity, quality and sustainability of perennial pastures in Southern Brazil. During the years of 2000 and 2001 the response of six temperate legumes (Adesmia latifolia (wild), Lotus corniculatus, L. subbiflorus, L. uliginosus and Trifolium repens cv. Yi and cv. Regal) was evaluated in mixture with tall fescue (Festuca arundinacea). There was a period of 475 days between sowing date and the last cut and six cuts were made; on the autumn-winter/2000 only one cut was made, on the spring-summer, three cuts and on the autumn-winter/2001, two cuts. The intervals between cuts ranged between 43 and 91 days, being reduced in the warm season. During the total assay period, the average of the mixtures yielded 15038 kg/ha of DM; in the warm season 54% of this total was produced, with an average growth rate of 46 kg/ha/day of DM. In the cold season of 2000 and 2001 this rate was of 22 and 24 kg/ha/day of DM. The birdsfoot trefoil and white clover cv. Yi were the most productive legumes; the former was the best in the warm season (3500 kg/ha of DM) and the last participated with 86,3% in the mixture in the autumn-winter/2001, with 2300 kg/ha/day of DM. The mixtures containing these legumes presented the smallest quantity of weeds and produced 13663 and 11,184 kg/ha of DM respectively, being 82% and 71% of these totals composed of legumes, fescue and ryegrass. A. latifolia did not have a good establishment and its contribution was only 0,84% in the first cut. The L. subbiflorus had a good establishment on the first year, but it disappeared from the mixture in the second year. L. uliginosus had a good establishment and persistence producing up to 1400 kg/ha of DM in the autumn/2001.

88 Lotus literature

SCHEFFER-BASSO S.M., VOSS M. e JACQUES A.V. 2001. Nodulação e fixação biológica de nitrogênio de Adesmia latifolia e Lotus corniculatus em vasos de Leonard. Rev. Bras. Zootec., 30, 687-693. [Scheffer-Basso S.M., Jacques A.V., Voss M. 2001. Nodulation and biological Nitrogen fixation of Adesmia latifolia and Lotus corniculatus in Leonard jars. Rev. Bras. Zootec., 30, 687-693. [Portuguese]] Adesmia latifolia is a forage legume native from Southern Brazil which is outstanding due to stoloniferous growth habit and for forage production during the cool season. The Lotus corniculatus (birdsfoot trefoil) is a forage usually utilized as cool season species in subtropical and temperate regions. For both species, however, there is a reduced number of scientific works related to the nodule type and biological nitrogen fixation (BNF). This work had the purpose to analyze such processes in these species and also to compare their morphological development under different nitrogen sources: mineral-N (ammonium nitrate - 5%), symbiotic-N (inoculation) and without nitrogen (control). The experiment was carried out in greenhouse conditions, utilizing Leonard Jars with nutritive solution; the substrate consisted of mixture of sand, vermiculite and charcoal. It was a randomized complete design with four replications. At the end of 65 days the plants were harvested and evaluated for length and volume of roots, number and weight of nodules, dry matter (DM) accumulation and BNF. A. latifolia stand out for the character number of nodules (126/jar) and total nodule weight (82.22 mg DM/jar) as compared to birdsfoot trefoil with 82 nodules/jar and 20.25 mg DM/jar. The BNF was more effective in A. latifolia, whose inoculated plants produced an average of 37% of DM that was obtained by plants supplied with mineral-N, while birdsfoot reached only 15% of DM production with BNF. The amount of symbiotic fixed nitrogen was 43.12 mg N/jar in Adesmia and 9.92 mg in birdsfoot trefoil.

SCHILLER K.N. and AYRES J.F. 1993. The effects of winter conditions on the nutritive value of Lotus pedunculatus cv. Grasslands Maku and Trifolium repens cv. Haifa. Tropical Grasslands Society of Australia Newsletter, 9, 12.

SCHILLER K.N. and AYRES J.F. 1993. The effects of winter conditions on the nutritive value of Lotus pedunculatus cv. Grasslands Maku and Trifolium repens cv. Haifa. Tropical Grasslands, 27, 43-47.

SKØT L., GORDON A.J., TIMMS E., JAMES C.L., WEBB K.J. and MIZEN S. 1997. Down- regulation of sucrose synthase expression and activity in transgenic hairy roots of Lotus japonicus. Symbiosis, 22, 241-254.

SKØT L., GORDON A.J., TIMMS E., WEBB K.J. and MIZEN S. 1996. Use of antisense RNA strategies to down-regulate sucrose synthase gene expression in transgenic Lotus japonicus. Emerging Model Legume Systems: Tools and Recent Advances. Knoxville, Tennessee, USA. 13-14 July 1996. Abs. No. 23.

SKØT L., GORDON A.J., TIMMS E., WEBB K.J. and MIZEN S. 1996. Use of antisense RNA strategies to down-regulate sucrose synthase gene expression in transgenic Lotus japonicus. Molecular Plant- Microbe Interactions. Knoxville, Tennessee, USA. 14-19 July 1996. Abs. No. 28.

SKØT L., GORDON A.J., WEBB K.J., TIMMS E., JAMES C.L. and MIZEN S. 1997. Down- regulation of sucrose synthase expression and activity in transgenic hairy roots of Lotus japonicus. Society of Experimetnal Biology Meeting. 7-11 April 1997. University of Kent, Lotus literature 89

Canterbury, U.K.

SKØT L., MINCHIN F.R., TIMMS E., FORTUNE M.T., WEBB K.J. and GORDON A.J. 1995. Analysis of the two nodulins, sucrose synthase and ENOD2, in transgenic Lotus plants. The First New Phytologist Conference: Molecular Approaches to the Study of Plant-Microbe Symbioses, York November 13-17.

SKØT L., MINCHIN F.R., TIMMS E., FORTUNE M.T., WEBB K.J. and GORDON A.J. 1995. Analysis of the two nodulins, sucrose synthase and ENOD2, in root nodules of Lotus spp. using antisense strategies. Fifteenth North American Conference on Symbiotic Nitrogen Fixation; North Carolina State University, August 13-17. Abstract no 79.

SKØT L., MINCHIN F.R., TIMMS E., FORTUNE M.T., WEBB K.J. and GORDON A.J. 1996. Analysis of the two nodulins, sucrose synthase and ENOD2, in transgenic Lotus plants. Plant and Soil, 186, 99-106.

SKØT L. and WEBB K.J. 1991. Engineering an insect pest resistance gene into legume root nodules. First International Conference on Bacillus thuringiensis 28-31 July 1991. St Catherine's College, Oxford UK. Abs. No. 48.

SKØT L. and WEBB K.J. 1991. Engineering an insect pest resistance gene into legume root nodules. 3rd AFRC Meeting on Plant and Soil Nitrogen Metabolism. UCW Aberystwyth. Abs. No. 19.

SKØT L. and WEBB K.J. 1991. Engineering an insect resistance gene into Rhizobium and legumes. 4th International Society of Plant Molecular Biology, Tucson, University of Arizona, USA. 6-12 October 1991. Abs. No. 1406.

SKØT L. and WEBB K.J. 1991. Engineering insect pest resistance into Rhizobium and legumes. Symbiosis and Crop Growth. The Rank-Prize Funds. Mini-Symposium on Symbiosis and Crop Growth. Molecular and Organismal Aspects. Organisers: The Nutrition Committee. Grasmere, Cumberland, UK. 30th April - 3rd May 1991.

SKØT L., WEBB K.J., NICHOLSON M.M., JØRGENSEN B. and MIZEN S. 1998. Use of T-DNA tagging to identify plant genes involved in symbiotic nitrogen fixation. 16th North American Conference on Symbiotic Nitrogen Fixation. Feb 1-6, 1998. Cancun, Mexico.

SKØT L., WEBB K.J., NICHOLSON M.M., JØRGENSEN B. and MIZEN S. 1999. Use of T-DNA tagging to identify plant genes involved in symbiotic nitrogen fixation. In: Martínez and Hernández. (Eds) Highlights of Nitrogen Fixation Research. 16th North American Conference on Symbiotic Nitrogen Fixation. 1- 6 Feb, 1998. Cancun, Mexico. 153- 156. Plenum Publishing Corporation, New York.

SOKOLOFF D.D. 1998. What is Tetragonolobus wiedemannii Boiss. (Fabaceae)? Novit. Syst. Pl. Vasc. (St. Petersburg), 31, 139-142. [Russian]. Tetragonolobus wiedemannii = Lotus wiedemannii is in fact a synonym of Hammatolobium 90 Lotus literature lotoides.

SOKOLOFF D.D. 1999. Typification of several specific and generic names in the tribe Loteae (Papilionaceae). Taxon., 48, 57-59. Several species of Lotus are typified. The species Lotus wrangelianus is moved into the genus Acmispon. The genus Syrmatium (a genus segregated from Lotus) is typified.

SOKOLOFF D.D. 1999. Ottleya, a new genus of Papilionaceae-Loteae from North America. Feddes Repert., 110, 89-97. The genus is segregated from Lotus and corresponds to Lotus sect. Simpeteria Ottley.

SOKOLOFF D.D. 2000. New combinations in Acmispon (Leguminosae, Loteae). Ann. Bot. Fennici., 37, 125-131. Acmispon is an American genus segregated from Lotus.

SOKOLOFF D.D. 2000. New combinations in Hosackia Douglas ex Benth. (Leguminosae: Loteae). Kew Bull., 55, 1009-1010. Hosackia is a North American genus segregated from Lotus.

SOKOLOFF D.D. 2001. New records of Lotus (Leguminosae: Papilionoideae: Loteae) from Africa and southwest Asia. Kew Bull., 56, 715-720.

SOKOLOFF D.D. 2003. On system and phylogeny of the tribe Loteae DC. (Leguminosae). Bull. Moscow Soc. Natur. Biol. Ser., 108, 35-48. [Russian]. The following new combinations and names in Lotus and its segregate genera are published: Hosackia sect. Protohosackia D.D. Sokoloff, Ottleya plebeia (Brandegee) D.D. Sokoloff, Ottleya mollis (A.A. Heller) D.D. Sokoloff et K.N. Gandhi, Lotus sect. Bonjeanea (Reichenb.) D.D. Sokoloff, Lotus sect. Dorycnium (Mill.) D.D. Sokoloff, Lotus axilliflorus (Hub.-Mor.) D.D. Sokoloff, Lotus fulgurans (Porta) D.D. Sokoloff, Lotus sanguineus (Vural) D.D. Sokoloff, Lotus sect. Canaria (Rikli) D.D. Sokoloff, Lotus sect. Rhyncholotus (Monod) D.D. Sokoloff.

Lotus literature 91

SOSTER M.T.B., SCHEFFER-BASSO S.M. e DALL’AGNOL M. 2003. Caracterização morfofisiológica de genótipos de cornichão (Lotus corniculatus L.). Submetido à Revista SBZ. [Soster M.T.B., Scheffer-Basso S.M. and Dall’agnol M. 2003. Morphophysiological characterization on the birdsfoot trefoil (Lotus corniculatus L.). Submeted to Revista SBZ. [Portuguese]] The birdsfoot trefoil cv. São Gabriel is an important winter-spring growing legume in the Southern Brazil, which main limitation is the low persistence under grazing. The objective of this work was to evaluate eight populations of this cultivar, selected under grazing or cut, comparing with two rhizomatous genotypes and a cultivar from Uruguay (Agrosan Trueno), with the purpose to evaluate the morphological variability and characters linked to grazing tolerance. Two assays were conducted in the greenhouse with plants grown in pots until the full flowering stage. It was observed morphological variability in the populations, with variation in the leaf, stem and crown morphology as well as in growth habit. Only in the rhizomatous genotypes were observed tipical rhizomes, but all the genotypes showed subterraneous stems. The rhizomatous genotypes were susceptible to mites and two populations showed symptoms of anthracnose. There are some morphophysiological variability of the cv. São Gabriel populations can be used in a birdsfoot trefoil breeding programs.

SOSTER M.T.B., SCHEFFER-BASSO S.M., DALL’AGNOL M., BRUSTOLIN R.E and FONTANELI R.S. 2003. Caracterização agronômica de genótipos de cornichão (Lotus corniculatus L.). Submetido à Revista SBZ. [Soster M.T.B., Scheffer-Basso S.M., Dall’agnol M., Brustolin R.E and Fontaneli R.S. 2003. Agronomic characterization if birdsfoot trefoil genotypes (Lotus corniculatus L.) This work was carried out to characterize agronomically birdsfoot trefoil (Lotus corniculatus L.) genotypes, including eight populations derived from the cultivar São Gabriel, one rhizomatous cultivar (ARS-2620) and one cultivar without rhizomes (Trueno). Individual plants were submitted to seven cuts under field conditions during one year, at Passo Fundo, Rio Grande do Sul, Brazil. There was little variation among the populations in relation to forage yield, quality and persistence. On the average, the populations and the cultivar Trueno were about 50% more productive than the cultivar ARS-2620. The persistence presented a range varying from 80% to 100% of survival. The crude protein content decreased from 21,8 (vegetative) to 11,2% (flowering). The overall mean for fiber insoluble in acid detergent was 24,2% and the fiber insoluble in neutral detergent ranged from 55,3% to 58,8%. Overall, the populations presented a higher yield potential in relation to the cultivar ARS-2620.

STENGLEIN S.A., COLARES M.N., ARAMBARRI A.M., NOVOA M.C., VIZCAÍNO C.E. and KATINAS L. 2003. Leaf epidermal microcharacters of the Old World species of Lotus (Leguminosae: Loteae) and their systematic significance. Australian Journal of Botany, 51, 1-11.

STENGLEIN S.A., ARAMBARRI A.M., COLARES M.N., NOVOA M.C. and VIZCAÍNO C.E. 2003. Epidermal characteristics of the New World species of Lotus: subgenus Acmispon (Fabaceae: Loteae) and a numerical taxonomic evaluation. Canadian Journal of Botany, 81, 933-944.

TIKHOMIROV V.N. and SOKOLOFF D.D. 1997. Taxonomic position of Vermifrux abyssinica (A. Rich.) Gillett and taxonomy of the tribe Loteae s.l. (Papilionaceae). Feddes Repert., 108, 335-344. The name Vermifrux was included by Polhill (1981) into synonymy of Lotus. We have 92 Lotus literature demonstrated that this genus should be merged with Dorycnopsis.

TSUTSUPA T.A., BARYKINA R.P., KRAMINA T.E. and SOKOLOFF D.D. 2001. On the reduction of terminal bud in seedlings of some papilionoid legumes // Feddes Repert., 112, 459-467. Morphological and anatomical structure of seeds and seedlings of Securigera securidaca (L.) Degen & Doerfl. and several other members of Loteae tribe [Ornithopus sativus Brot., Lotus tetragonolobus L., Tripodion tetraphyllus (L.) Fourr. and Hymenocarpus circinnatus (L.) Savi] was studied. Development of the plumule, cotyledons, shoots system and main root of the seedlings are described with special attention to the vascular system. In cotyledonary axils, serial buds early appear and develop that makes identification of the main shoot rather difficult. However, the apical bud and normally developed main shoot were found in all examined species. Dormer’s (1945) idea on the reduction of the main bud and Compton’s (1912) supposition on extra-axillary branching of epicotyl into equivalent shoots were disproved.

TUCK E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2001. Signal transduction during early symbiotic events (2001). Molecular Genetics of Model Legumes: Impact for legume Biology and Breeding 15-19th Sept 2001 109.

TUCK E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2002. Symbiosis- specific expression of a gene encoding a calcium-binding protein in the model legume Lotus japonicus during early interactions with root-nodulating bacteria (Mesorhizobium loti) and the arbuscular mycorrhiza fungi Glomus mosseae. Plant Science Wales 7-8th January, Bangor. Abs no. 4.

TUCK, E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2002. Expression of a gene encoding a calcium-binding protein in the model legume Lotus japonicus during early interactions with root-nodulating bacteria (Mesorhizobium loti) and the arbuscular mycorrhiza fungi Glomus mosseae. Society of Experimental Biology April 2002 Swansea. Abs no. P8.2.

TUCK E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2002. Identiying symbiosis-specifc genes in the model legume Lotus japonicus. Ist International Conference on Legume Genomics and Genetics. Minneapolis USA. June 2002. PM17

TUCK E., MUR L., SKØT L., COOK R., PARNISKE M. and WEBB K.J. 2000. Early gene expression during plant-microbe interactions. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. P48.

WANG T.L., DOMONEY C., HEDLEY C.L., CASEY R. and GRUSAK M.A. 2003. Can we improve the nutritional quality of legume seeds? Plant Physiology, 131, 886-891.

WEBB K.J. 1985. Transformation of the forage legumes using the natural gene vector, Agrobacterium tumefaciens. White Clover Discussion Group meeting, AGRI Hurley 1- 2 August 1985.

Lotus literature 93

WEBB K.J. 1986. Transformation in the forage legumes. Genetic Manipulation in Plant Breeding. Eds: W Horn, CJ Jensen, W Odenbach and O Schieder. Proc. International Symposium organised by Eucarpia. 8-13 Sept 1985. Berlin (West) Germany. 831-833.

WEBB K.J. 1986. Transformation of forage legumes using Agrobacterium tumefaciens. Theoretical Applied Genetics, 72, 53-58.

WEBB K.J. 1988. Recent developments in the regeneration of agronomically important crops from protoplasts. Plant Cell, Tissue and Organ Culture, 12, 127-131.

WEBB K.J. 1990. Transformation in herbage legumes. UCW Cell Genetics Group Meeting UK 8th-9th January 1990.

WEBB K.J. 1996. Opportunities for Biotechnology in Forage Legume Breeding. Legumes in Sustainable Farming Systems. British Grassland Occasional Symposium No. 30. 2-4 September Aberdeen, Scotland. Ed D Younie. pp. 77-85.

WEBB K.J. and ARMSTEAD I.P. 1986. Transformation in the herbage legumes. IAPTC, UK Symposium, Edinburgh, UK. 1986.

WEBB K.J., CHAMBERLAIN D.A., WOODCOCK S. and DALE P.J. 1986. Cell and protoplast culture in forage legumes. In: Genetic Engineering of Plants and Micro-organisms 88-89.

WEBB K.J., EASON W., ABBERTON M., MICHAELSON-YEATES T., TEODSIO H.R., PARNISKE M. and HOOKER J. 2000. Tools for unravelling genetic control of arbuscular mycorrhizal symbiosis in legumes.

WEBB K.J., FAY M.F., WOODCOCK S., PIKE L.S. and DALE P.J. 1985. Protoplast culture of forage legumes. In: Genetic Engineering of Plants and Micro-organisms Important for Agriculture. Eds: E Magnien & D de Nettancourt. Martinus Nijhoff 162-163.

WEBB K.J., FAY M.F., PIKE L.S., WOODCOCK S. and DALE P.J. 1984. Selection of responsive genotypes for protoplast culture in forage legumes. British Grassland Society Occasional Symposium No 16. In: Forage Legumes. Ed: DJ Thomson 168 -169.

WEBB K.J., FAY M.F., JONES M.G.K., PIKE L.S., WOODCOCK S. and DALE P.J. 1983. Protoplast culture in forage legumes. Research and training programme in Biomolecular Engineering, Louvain-la-Neuve, Belgium. Abstract no 83.

WEBB K.J., FAY M.F., JONES M.G.K., PIKE L.S., WOODCOCK S. and DALE P.J. 1983. Protoplast culture in forage legumes. AFRC Genetic Manipulation of Crop Plants: Five years on 12-13 December , University of Cambridge, UK. Abstract no 76.

WEBB K.J., GIBBS M., MIZEN S., SKØT L. and GATEHOUSE J. 1996. Genetic transformation of Lotus corniculatus with Agrobacterium tumefaciens and the analysis of the inheritance of trangenes in the T1 generation. Transgenic Research, 5, 303-312. 94 Lotus literature

WEBB K.J., HUMPHREYS M.O., SKØT L., GIBBS M. and GATEHOUSE J. 1999. Inheritance and expression of transgenes in T2 and T3 generations of Lotus corniculatus transformed using Agrobacterium tumefaciens. Euphytica, 108, 169-179.

WEBB K.J., JONES S. and GIBBS M. 1990. Use of Agrobacterium rhizogenes binary vector to transform herbage legumes. VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam 24-29 June 1990. Abstr. no.A2-147.

WEBB K.J., JONES S., ROBBINS M.P. and MINCHIN F.R. 1990. Characterization of transgenic root cultures of Trifolium repens, T.pratense and Lotus corniculatus and transgenic plants of L.corniculatus. Plant Science, 70, 243-254.

WEBB K.J., JØRGENSEN B., SKØT L. and DOWNIE J.A. 1994. Evaluation of Lotus japonicus for a T-DNA tagging programme. PMBII Mid-term Meeting, University of East Anglia, 6-8th July, Norwich.

WEBB K.J., MEREDITH M.R. and NISBET G.S. 1986. Study of variation in regenerants of Lotus corniculatus. IAPTC, UK Symposium, Edinburgh, UK. 1986.

WEBB K.J., MIZEN S. and COOKE D.E. 1994. A long term study of GUS activity in hairy root cultures and primary transformants in Lotus corniculatus. LOTUS Newsletter. Ed: PR Beuselinck USDA-Agricultural Research Service. 25:28-30.

WEBB K.J., MIZEN S., ROBBINS M.P., GATEHOUSE J.A. and ROWE M.J. 1994. Patterns of inheritance of transgenes in the herbage legumes Lotus corniculatus transformed by Agrobacterium rhizogenes and A.tumefaciens. VIII International Congress on Plant Tissue and Cell Culture. Firenze, 12-17 June 1994.

WEBB K.J. and MORRIS P. 1992. Methodologies of Plant Transformation. In: Gatehouse A., Hilder V.A. and Boulter D. (Eds) Plant Genetic Engineering for Crop Protection. CAB International. pp. 7-43.

WEBB K.J., NICHOLSON M.M., JØRGENSEN B., MIZEN S. and SKØT L. 1996. T-DNA mutagenesis in Lotus japonicus. Emerging Model Legume Systems: Tools and Recent Advances. Knoxville, Tennessee July 12-14, 1996. Abs. No. 5.

WEBB K.J. and RHODES I. 1991. Opportunities for biotechnology in clover breeding. Fodder Crops Breeding: Achievements Novel Strategies and Biotechnology. Proceedings of the 16th Meeting of Eucarpia, Wageningen, The Netherlands. Eds: APM den Dijs and A Elgersman. Pudac, Wageningen. 18-22 November 1990. pp. 111-116.

WEBB K.J., ROBBINS M.P. and MIZEN S. 1993. Expression of GUS in primary transformants and the inheritance of GUS, TL and TR in progeny of Lotus corniculatus L. International Symposium on Genetic Manipulation of Plant Metabolism and Growth. University of East Anglia, Norwich, UK. 29-31 March 1993. Abstract no 1. Lotus literature 95

WEBB K.J., ROBBINS M.P. and MIZEN S. 1994. Expression of GUS in primary transformants and segregation patterns of GUS, TL- and TR-DNA in the T1 generation of hairy root transformants of Lotus corniculatus. Transgenic Research, 3, 232-240.

WEBB K.J., ROBBINS M.P. and MIZEN S. 1994. Segregation of Agrobacterium rhizogenes T- DNA from other inserted genes in the T1 progeny of Lotus corniculatus. Proceedings First International Lotus Symposium, 22-24 March 1994, St Louis, Missouri, USA. Ed: P Beuselinck.

WEBB K.J., ROBBINS M.P. and MIZEN S. 1994. 1st International Symposium, St Louis, USA. February 1994.

WEBB K.J. and SKØT L.. 2000. A calcium binding protein homologue identified using promoter tagging in Lotus japonicus. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No.S51.

WEBB K.J., SKØT L., COOKE D.E., GIBBS M.J., MIZEN S. and ROBBINS M.P. 1995. Expression and inheritance of transgenes in Lotus corniculatus. XIV EUCARPIA. Adaptation in Plant Breeding. Jyväskylä, Finland. July 31-Aug 4. p121.

WEBB K.J., SKØT L. and DOWNIE A. 1994. Identification of plant genes specifically involved in nodulation and nitrogen fixation. PMBII Mid-Term Meeting 6-8th July 1994.

WEBB K.J., SKØT L. and DOWNIE J.A. 1994. Identification of plant genes specifically involved in nodulation and nitrogen fixation. PMBII Mid-term Meeting, University of East Anglia, 6- 8th July, Norwich.

WEBB K.J., SKØT L. and JØRGENSEN B. 1994. Plant genes involved in nodule development and nitrogen fixation. Proceedings of the 1st European Nitrogen Fixation Conference, Szeged, Hungary. August 29 - September 2. Eds: GB Kiss and G Endre. Officina Press. pp. 234-238.

WEBB K.J., SKØT L., and JØRGENSEN B. 1995. Progress in mutagenesis, mapping and carbon metabolism in Lotus japonicus. EU Project of Technological Priority. Joint Meeting of Themes D1 and D2. 2nd Joint Meeting on Nitrogen Utilization Efficiency. September 1995, Sevilla, Spain.

WEBB K.J., SKØT L., MIZEN S., PARNISKE M., JØRGENSEN B. and NICHOLSON M. 1998. Identification of genes expressed in roots and nodules of Lotus japonicus using a promoter trap. Plant Biotechnology and In vitro Biology in the 21st Century. IX International Congress on Plant Tissue and Cell Culture, Jerusalem, Israel, 14-19 June 1998. p. 44.

WEBB K.J., SKØT L., MIZEN S., PARNISKE M., JØRGENSEN B. and NICHOLSON M. 1998. Using EMS mutagenesis and promoter trapping to search for mutants and promoters in symbiotic interactions of Lotus japonicus. First International Lotus japonicus Workshop. 96 Lotus literature

20-22nd November 1998, University of Aarhus, Denmark.

WEBB K.J., SKØT L., MIZEN S., PARNISKE M., JØRGENSEN B. and NICHOLSON M. 1999. Identification of genes expressed in roots and nodules of Lotus japonicus using a promoter trap. In: Altman A., Ziv M. and Izhar S. (Eds) Current Plant Science and Biotechnology in Agriculture. Plant Biotechnology and In vitro Biology in the 21st Century. IX International Congress on Plant Tissue and Cell Culture, Jerusalem, Israel, 14-19 June 1998. 227-229.

WEBB K.J., SKØT L., NICHOLSON M.N., JØRGENSEN B. and MIZEN S. 2000. Mesorhizobium loti increases ot-specific expression of a calcium-binding protein homologue identified by promoter tagging in Lotus japonicus. Molecular Plant-Microbe Interactions, 13 (6), 606- 616.

WEBB K.J. and WATSON E.J. 1991. Lotus corniculatus L.: Morphological and cytological analysis of regenerants from three sources of tissue and selected progeny. Plant, Cell, Tissue and Organ Culture, 25, 27-33.

WEBB K.J., WOODCOCK S. and CHAMBERLAIN D.A. 1987. Plant regeneration from protoplasts of Trifolium repens and Lotus corniculatus. Journal of Plant Breeding, 98, 111-118.

WEBB K.J., WOODCOCK S., PIKE L.S. and DALE P.J. 1986. Plant regeneration in the forage legumes. In: Plant Tissue Culture and its Agricultural Applications. Eds: LA Withers and PG Alderson. 17-21 Sept 1984. Butterworth Scientific, London. 99-104.

WEBB K.J., WOODCOCK S., PIKE L.S., FAY M.F. and DALE P.J. 1984. Protoplast culture in forage legumes. University of Wales Staff Colloquium. Biotechnology 20-21 March.

WILSON G.R.M. and ORAM R.N. 1991. B. Legumes 22. Lotus (a) Lotus pedunculatus Cav. (greater lotus) cv. Sharnae. Register of Australian herbage plant cultivars, pp 794-795.

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