NITM/BGV/LE/16-17/ Due On: 13Th March 2017 at 11.00 AM

TENDER ENQUIRY

Enquiry No. ICMR - NITM/BGV/LE/16-17/ Due on: 13th March 2017 at 11.00 AM

Sealed tenders are invited for supply, erection, testing, commissioning of the following scientific equipments from reputed manufacturers (Indian/Foreign) or their authorized agents / counter parts in India duly registered, on two- bid system i.e. technical and financial. Detailed specifications of the requirements are enclosed as Annexure–II.

All installations will be at ICMR – National Institute of Traditional Medicine (formerly Regional Medical Research Centre), Nehru Nagar, Belagavi 590010.

S. No. Details 1. EMD Amount as per the Annexure–I 2. List and specification enclosed as per the Annexure–II 3. Please refer terms and conditions as per Annexure– III 4. Proforma for Bank Guarantee for Performance Bond / Security Deposit Annexure– IV

The envelopes should contain the following documents: Envelope1: EMD D.D. Amount Envelope2: Technical bid including conditions of supply and maintenance

Envelope3: Commercial bid/Financial bid

All these envelopes should be sealed and super scribed (as mentioned in the terms and conditions) in the separate cover. Separate quotation should be sent for each equipment to The Director, ICMR – National Institute of Traditional Medicine - NITM (formerly Regional Medical Research Centre), Nehru Nagar, Belagavi 590010.

Director

Annexure-I

ICMR – National Institute of Traditional Medicine (NITM), BELAGAVI – 590010

EMD Amount for the following Equipment

EMD to Sl be Brief Particulars of Equipment Quantity No. submitted (Rs)

1 Nanodrop spectrophotometer 1 45,000

2 Spectrophotometer 1 30,000

3 PCR Thermo cycler 2 30,000

4 Digital automatic autoclave 5 22,500

5 Incubator shaker 2 15,000 Gel System Vertical for protein/isozyme studies 6 1 (Medium size) with all accessories and power pack 660 Gel System Horizontal for DNA studies (Medium 7 1 size) with all accessories and power pack 600

8 Water bath shaker 2 9,000

9 Hot air oven 1 3,000

10 Air cleaning / purification system 1 6,000

11 Bio safety cabinet (Type II, Class A2) 3 36,000

12 Controlled Environment chamber 1 24,000 Animal care equipment (Individually ventilated 13 2 Animal caging system) 1,44,000

14 Rabbit cages with rack unit 1 30,000 Metabolic and Diuresis cages for rodents with rack 15 1 unit 16,500

16 Micro injector system 1 19,500

17 Gas Anaesthesia equipment for rodents 1 12,000 Needle electrodes and Data acquisition system for 18 1 ECG recording 18,000

19 Zebra fish aquatic housing system (recirculating) 1 30,000

20 Zebra fish cardiac activity imaging tracking system 1 60,000

21 Biochemical semi auto analyser 1 12,000

22 Bench top multipurpose Centrifuge 1 3,000

23 pH/Conductivity/TDS meter (multiparameter) 1 3,000

24 Animal Surgical platform 1 3,000

25 Tissue homogenizer 1 4,500

26 In vivo imaging System 1 22,500

27 Gel Electrophoresis unit Horizontal 1 600

28 Animal Bedding disposal station 1 30,000

29 Vacuum pump for lyophilizer 1 3,000

30 HPLC- Analytical 1 1,05,000

31 Flow cytometer with sorter 1 1,80,000

32 Ultracentrifuge 4 3,60,000

33 Confocal microscope with live cell imaging system 1 1,80,000

34 Inverted epifluorescence microscopeAxiovar 40 1 54,000

35 Multimode micro plate reader & Washer 1 75,000

36 Real Timer QRT PCR 1 72,000

37 CO2 incubator 28 C 1 ° 30,000

38 CO2 incubator 37 C 1 ° 30,000

39 Dissecting Microscope 1 12,000

40 Analytical Micro balance 1 4,500 41 Adjustable volume single channel pipettes 1 600

Note: 1. Demand Draft should be in the name of The Director, ICMR – National Institute of Traditional Medicine (NITM), payable at BELAGAVI.

Annexure-II DETAILED SPECIFICATIONS

Specifications of Instruments 2016-17 ICMR-NATIONAL INSTITUTE OF TRADITIONAL MEDICINE, BELAGAVI

SPECIFICATIONS OF EQUIPMENTS 2016-2017

1. Nanodrop spectrophotometer

Application For estimation of Nucleic Acids at A260, A260/A280, A260/A230 and Labeled Nucleic Acids; Protein A280 and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Labeled Proteins, OD600, Kinetics, UVVis, and Custom Methods Instrument Control Built-in touchscreen Minimum Sample Volume 1 μL Limit of Detection dsDNA Pedestal 2.0 ng/μL Pedestal 0.06 (0.03) mg/mL Maximum Concentration ds DNA Pedestal 27,500 ng/μL BSA (IgG) Pedestal 820 (400) mg/mL Measurement and Data Processing Time 8 seconds Measurement Repeatability Typical 0.002 A (1.0 mm path) or 1%CV, whichever is greater Wavelength Range 190–850 nm Accuracy ±1 nm Photometric Range (10 mm equivalent) Pedestal: 0550A Resolution (Spectral Bandwidth) ≤1.8 nm Pathlength: 0.030 to 1.0 mm Light Source Xenon flash lamp Detector 2048-element CMOS linear image sensor On-Board Control Operating System Android™ or better CPU Quad Core ARM® Cortex™-A9 Processor or better Display 7-inch, 1280 × 800 high-definition color display Touchscreen Multipoint capacitive touch Gesture Recognition Single point, single point hold, swipe and pinch Glove Compatibility Compatible with lab gloves Internal Storage >32 GB flash memory Audio Built-in speaker Connectivity Three USB-A ports, Ethernet, Bluetooth and Wi-Fi3 PC Software Requirements Windows® 10 Accessory Support: Printer, Bluetooth keyboard, mouse and barcode reader. Warranty 5 year comprehensive

2. Spectrophotometer:

Quantitation of DNA, RNA, and oligonucleotides, Quantitation of proteins via the Bradford, Lowry, and BCA Applications assay methods, Monitoring bacterial culture growth, Simple kinetic assays, Wavelength scans with peak detection Light souce Xenon lamp Detector Photodiode array Display 2 x 24 alphanumeric backlit LCD Inputs Menu driven keypad Users, Up to 10 standard curves can be stored under user- assigned names. Standards should be analyzed in groups of up Memory to 9 replicates. Sample concentration display in µg/ml (additionally in pmol/µl for oligonucleotides) Printer Built-in alphanumeric/graphic printer on board Wavelength range 200–800 nm Wavelength accuracy (at 18–27°C) <±1.0 nm from 200 to 250, 300 to 800 nm <±0.5 nm from 250 to 300 nm Spectral bandwidth <5 nm Absorption accuracy ±0.01 at 0.5 AU, ±0.02 at 1.0 AU Absorption repeatability ±0.005 at 0.5 AU Stray light <0.2% at 230 nm using Nal standard Operating temperature 15–35°C Certification CE certified Warranty Comprehensive 5 year

3. PCR Thermocycler Format 3 x >30 well independent control blocks Sample capacity 0.2ml tubes or strips PCR volume 10- 80 ul Control keys and display Colour TFT LCD touchscreen display and input Memory Stores minimum 50 methods, USB, onboard Temperature range 0–99.9ºC Max block ramp rate Upto 6°C/sec. Max sample ramp rate > 4°C/sec Static temperature uniformity <0.5°C (20 sec after reaching 95°C) Temperature accuracy ±0.25ºC (range: 35–100ºC) Heated cover Should maintains constant temperature of 105ºC for oil-free operation Ramp time reproducibility Should reach thermal set points within ±5 seconds Block replacement Option of fitting block with 96 well X 0.2 ml Block homogeneity Should be + / _ 0.3° C Auto restart Should be available after power resumption Data transfer Via USB Warranty 5 year comprehensive

4. Digital automatic Autoclave

Sterilizing 105 ∼ 135°C（0.019 ∼ 0.212MPa） Operating temperature Heating 45 ∼ 104°C（0 ∼ 0.015MPa） range Warming 45 ∼ 95°C Maximum operating pressure 0.263MPa Temperature Display Digital Display Analog Pressure gauge Display range 0 ∼ 0.4MPa Heat source 2kW electric heater Water level sensor, Current leakage breaker, Lid interlock, Over-heat Safety device prevention, Over-pressure prevention, Open temperature sensor detection, Safety valve Display Digital, programmable Sterilizing 0:00 - 9:59, 0 – 99 hr, 0 – 999 min.(0:00 - Time Display Heating 9:59,10 – 99 hr changeable) range Warming Variable: 0 - 99hours Effective internal Chamber capacity volume:50L, Internal volume:58L Chamber material SUS304 Should have provision of circulation of steam back to water Should operate on only one water inlet and Site specific installation requirements should not require any drainage system. Should be easily movable for easy placement to various locations in the laboratory. Warranty Comprehensive 5 years

5. Incubator shaker

Temperature Ambient + 5°C to 75°C Storage and footprint Stackable Capacity >200 litres. Should holds flask up to approx 6 - 7L Speed Approx 50 - 400 rpm Operation Continuous and timed operation Display LED, should indicate time, temperature and speed Shelves Min one for incubation Housing Triple eccentric counterbalanced drive made of heavy duty cast iron Other features 1. Built in water reservoir to prevent sample loss due to evaporation 2. Auto-restart function 3. It should auto shut off when the high temperature limit is exceeded 4. Should have a large viewing window Platform Universal platform with clamps for 250ml (5 nos), 500ml (5 nos), 1 L (5 nos), 25 ml (10 nos) Certification Should be CE / UL certified Power and Should be eco-friendly and power saving (details of power consumption to environment be provided) Warranty 5 year comprehensive

6. Gel System Vertical for protein/isozyme studies (Medium size) with all accessories and power pack

Type Vertical, Mini, Wing-clamp assembly No of gels Two simultaneously Glass Plates 5 sets About 10 X 7 cm (notched) and 10 X9 cm for spacer Buffer volume in tank 700 ml approx for 2 gels Run time for SDS-PAGE 45 mins approx at 200 V constant Combs 10 well, 1.0 mm thickness, one preparative Casting stand 2 frames Tank With buffer level marks, sample loading guide, electrode assembly, lid with power cables, mini- cell buffer dam Should contain a western blotting module Should be quoted with a cooling unit to absorb Should be used stand-alone apparatus and also a heat generated during quick transfer protocol as module. add-on Power-pack Should be quoted along with suitable power pack of the same brand Warranty 5 year comprehensive

7. Gel System Horizontal for DNA studies (Medium size) with all accessories and power pack 1 Type Horizontal Cell 2 Buffer capacity 1.2 Lit approx 3 Sample capacity Upto 550 sample per gel for large tray 4 Gel run length Approx 25 cm for large tray 5 Tray nos. & sizes Three (25 cm X 20 cm; 20 cm X 20 cm; & 10 cm X 20 cm) Corresponding to standard blotter sizes Combs Multichannel pipette compliant 0.75 mm thick, Prep 1+ marker 1 X 1no 0.75 mm thick, Prep 2+ marker 2 X 1no 0.75 mm thick, Prep 4+ marker 2 X 1no 0.75 mm thick, 10 samples X 1no 0.75 mm thick, 16 samples X 1no 0.75 mm thick, 20 samples X 1no 0.75 mm thick, 25 samples X 1no 0.75 mm thick, 30 samples X 1no 0.75 mm thick, 36 samples X 1no 0.75 mm thick, 40 samples X 1no 0.75 mm thick, 50 samples X 1no 1.0 mm thick, Prep 1+ marker 1 X 1no 1.0 mm thick, Prep 2+ marker 2 X 1no 1.0 mm thick, Prep 4+ marker 2 X 1no 1.0 mm thick, 10 samples X 1no 1.0 mm thick, 16 samples X 1no 1.0 mm thick, 20 samples X 1no 1.0 mm thick, 25 samples X 1no 1.0 mm thick, 30 samples X 1no 1.0 mm thick, 36 samples X 1no 1.0 mm thick, 40 samples X 1no 1.0 mm thick, 50 samples X 1no 7 Accessories Casting tray, Buffer dams, Gel scoops 10 Power pack  Voltage:0-400V  Current :0-300 mA  Power:0-50W  Timer:0-99.59 hrs  Display: LCD  Programmable: 9x9 set of parameters  Output: 3 in parallel sockets 11 Warranty Comprehensive 5 year warranty

8. Water bath shaker

Operable temperature range RT+ 50Cto 600C Shaking method Reciprocal shaking motion Temperature setting range 100Cto 800C Accuracy ±0.10C or less (at 370C) Shaking speed 20 to 160 times/min (Feed back control) Temperature control Microprocessor P.I.D. control Auto-tuning function Temperature setting & display Membrane key pad, Increment 0.10C, Digital setting & read-out Shaking speed setting & display Membrane key pad, 0 to 180rpm (Increment 1 rpm), Digital setting & read-out Timer setting & display Membrane key pad, 0 to 99 hr 59min (Increment 1min), Digital setting & read-out Program Auto-start, Auto-stop, Step program Safety feature Self-diagnose function (Heater fault, Sensor fault, Upper/Lower temperature limit alarm, Shaker motor fault) Empty boil protector with float, Independent overheat protector (fixed), Breaker for electrical leakage and excess current, Fuse Other features Temperature compensation, Auto-tuning, Alarm buzzer, Power failure detector Sensor PT Shaker motor Induction motor Stirring of bath Water jet Suitable for at a time shaking of: Ø12mm test tube, 180 pcs Ø16.5mm test tube, 90 pcs 50mL centrifuge tube, 30 pcs 50mL Erlenmeyer flask, 20 pcs 100mL Erlenmeyer flask, 12 pcs 200mL Erlenmeyer flask, 8 pcs 300mL Erlenmeyer flask, 6 pcs 500mL Erlenmeyer flask, 4 pcs Shaking basket Spring shelf x 2 pcs. + Bottom plate Accessories Should include all accessories required Stabilizer Should be supplied with suitable stabilizer, if recommended Power 220 V; 50 Hz Warranty 5 years comprehensive After sales service Should quote AMC for 6th to 10th year as percentage of basic cost of equipment

9. Hot air oven

Temperature range 5 above ambient -180ºC Accuracy ±2ºC upto 80ºC, ± 1ºC from 80ºC to 180ºC Control Thermostatic Capacity 45 x 45 x 45 cms Wall and Material Should be made of double walled chamber - Inner made of stainless steel SS 304 grade and powder coated outer surface. Shelves S S Shelves, 2 nos with holes Heating elements Should provide three heating elements on three sides of the equipment for uniform temperature on all shelves. Circulation Should be provided with air circulating fan Controls and display Variable microprocessor based digital temperature controller with digital display Thermometer Provided separately Power input 230V, 50Hz single phase AC Warranty 5 year comprehensive

10. Air Cleaning/Purification System Air purification system for the control of allergens, animal dander, viruses, bacteria and ammonia gas etc. in the air. Specifications:  Built-in high efficiency centrifugal blower for AirFlow: 265 cfm.  System should be with germicidal UV lamp with light emission approximately 254 nm  Higher Filter Efficiency for particle size 0.30 micron  Activated Charcoal gas absorption  Very low noise level (<63.5 dBA)

Warranty: 5 year comprehensive

11. Biosafety cabinet Specifications:  Cabinet should be class II type A2  Internal Dimension (W x D x H) should be -1200 x780 x630 mm approx.  Should have vertical Laminar Air flow with UV-lamp  Dual long life HEPA filter for supply and exhaust with 99.995% efficiency for particle size between 0.1 to 0.3 microns to provide around 70% down flow Air and 30% exhaust with under pressure condition in working area  Working area should match requirement for work with agents assigned to Biosafety level 1, 2&3.  Frameless front window and sides are non-reflecting safety glass with height limit alarm for front window  Very low noise level (<63.5 dBA) and vibration free  High capacity centrifugal fans for impulsion and exhaust  Control system with visual and buzzer alarm with cabinet information  Meet American (NSF/ANSI) or European standard EN 12469 (type tested) or both  Power Supply of 220V/50Hz  Cabinet should have light Intensity>1400 Lux  Main body should made up of electro-galvanized steel with, white oven-backed epoxy powder-coated finish  Should have Ergonomically Sloped front  Cabinet Should have drain out system inside the work top  System should be with built-in high efficiency centrifugal blower, germicidal UV lamp with light emission approximately 254 nm, choke-less fluorescent lamps, service fixtures for gas/vacuum/air inlet and electrical sockets.

Warranty: 5 year comprehensive

12. Controlled environment chamber Specifications:  Chamber Volume approx. 300L / 10cu ft  Dimension (mm) (WxDxH): approx. 500×550×1100  Illumination: 0-2000 lux with uniformed luminescence distribution  Temperature: 20-50ºC  Humidity: 40 to 80% RH at (at 20~35 ºC)  Shelves: 10Number

Warranty: 5 year comprehensive

13. Animal care equipment (Individually ventilated Animal caging system) Individually Ventilated Cage (IVC) system consisting of the following items: Air Handling Unit, Rack Single Side (56 cages), Cage complete with feeding bottles Specifications:  Rack dimensions: L 1400-2200mm, W 400-900mm, H 1700-1900mm with capacity for 56cages  Compatible AHU and necessary connections  HEPA filtered supply and exhaust air  No. of Cages 56 Individually ventilated cages with the unit  Filtered seal safe top  Even air diffusion into each cage  Water bottle should be replaceable without opening the cage  Nylon seal and quick release clamp between cover and cage base  Full visibility in cages  Direct exhaust from the cage that should provide enhanced ventilation efficiency  Protection of animals against equipment disorder and electricity breakdown  Cages should be compatible to house mice infected with infectious agents like Herpes simplex virus  Protection of animals and research personnel from cross-contamination  Barrier system for each cage

Warranty: 5 year comprehensive

14. Rabbit cages with rack unit Stainless Steel Complete Rabbit Cage Rack System. Specifications:  Self-contained removable cages (Six numbers) suspended on three-tier rack system.  Rack size (WxDxH) inches: Approx.60x25x75  Cage size (WxDxH) inches: Approx.24x24x20  The unit should be have Food hopper, provision for Bottle Watering, excreta tray and casters.

Warranty: 5 year comprehensive

15. Metabolic and Diuresis cages for rodents with rack unit Complete metabolic rack system with under shelves on each row. Eight Rat cages units with interchangeable parts for mice. Specifications:  Stainless steel rack to accommodate eight cages.  Rack size (WxDxH) inches: Approx.50x20x70  Complete cage system for rats/mice and each cages positioned on removable shelf.  The unit should be have food hopper/feeder, water bottle holder, water bottle, urine deflector, urine collection funnel and vessel, and casters.

Warranty: 5 year comprehensive

16. Microinjection System: A menu-controlled, programmable micromanipulator microinjector system should be ideally suited for manual and semiautomatic microinjection into and for serial microinjections into fish larvae, embryos, etc. Specifications: 1. Micromanipulator:  Integrated coarse & fine manipulator with proportional control via central joystick.  Work range 20mm per axis  Automated axial injection movement with programmable z-axis.  Automated home function for rapid capillary exchange.  Displayable menu control 2. Micro injector:  Programmable micro injector with external pressure supply  Application: Microinjection into small organisms or embryos at early developmental stages  Serial injection volumes from Picolitres to Microlitres  Special Features:  Choice of automatic or manual injection for maximum flexibility.  Simple and rapid adjustment of parameters most frequently modified: compensation pressure, injection pressure and time.  Automatic cleaning function.  Injection time: 0.0- 99.9 sec; can be set in increments of 0.1 sec 3. Foot switch and other accessories 4. Apochromatic stereo Zoom Microscope compatible for micromanipulator with illuminator, full range optics, cables and software’s 5. Adapter for Microscope. 6. Needle puller. Warranty: 5 year comprehensive

17. Gas Anesthesia equipment for Rodents Isoflurane Table Top Anaesthesia Unit for Rat/Mice The complete anesthesia systems with all the parts, regulators, tubing and connectors, flowmeter, vaporizer, induction chamber/box needed for induction of anaesthesia in mice and rats Specifications:  Anesthesia Machine with 0.2 to 4.0 litres per minute O2 flow with an attached absorber system for re-breathing system.  System should be preferably powder coated to keep it clean & chip free. The machine should have such a design such as it minimizes the use of hose and fittings and reduces chances of leakages of anesthetic gases. It should also have provisions for conversion for rebreathing/non-rebreathing circuits.  The system should include a vaporizer for isoflurane (anesthetic agent). The vaporizer should have an appropriate knob (preferably funnel knob) having ease of handling and provide controlled amounts of the gas anesthetic under standard ranges of delivery to the oxygen that is flowing through the machine. The vaporizer should include end caps for tubing attachment.  The system should also provide absorber canisters along with connection tubing for scavenging of excess anesthetic gas from the working area.  The system should include appropriate gas anesthesia masks for Rats and Mice with Diaphragm should have an appropriate shape that fit to the facial contours of the animal's snout.

Warranty: 5 year comprehensive

18. Needle electrodes & data acquisition system for ECG recording Data acquisition system with needle electrodes for ECG recording in Zebrafish Specifications:  The Data Acquisition system should have ability to record on 8 channels simultaneously and should be capable of recording up to 200K samples per second on each channel (400K samples per second aggregate) with 16 bit resolution  Stimulation Output range should be at least: ±200 mV to ±10 V full scale in six steps and Maximum output current ±50 mA  Communication with a high-speed plug and play USB only and it should allow variable sampling rates per channel.  The software should record and provide special advanced analysis feature for online and offline analysis. It should allow software controlled sampling rates, range and filter setting.  It should be upgradable to continuously record and display up to 32 channels of data (up to 32 calculated signals).It should include automated analysis and interpretation tools such as beat classifier, averaging, water fall plot, Dose response (EC50) curve plots and many other automatic analysis features.  It should allow user to export, MATLAB, Excel, Graph Pad Prism, Text etc.  It also has an option for file sharing with distinct user free of cost.  Software should have free update & upgrades for 5 years.  Dual channel Bio-amplifier with suitable needle electrodes for measuring bio-potential (ECG, EMG, EEG etc) in Zebra fish along with ECG, HRV, BP analysis software.  A highly sensitive physiological pressure transducer should be supplied for monitoring of beat to beat Blood Pressure analysis.  Software controlled two channel bridge amplifier for measuring force, displacement, and pressure with automatic zeroing circuitry.  Fluid less micro-tip Blood pressure catheter of 2F should be provided along with the system.  The system should be supplied with the branded PC with latest configuration, with at least i5 processor, 4 GB or better RAM, 1TB HDD, DVD RW, Genuine Windows OS,  Monitor 20”, keyboard and mouse should be provided. UPS 2KvA.  Accessories: 2 set of needle electrodes, Tabletop farade cage (1.5x1.5x1.5) ft, Tissue bath.

Warranty: 5 year comprehensive

19. Zebrafish aquatic housing system (recirculating) Aquatic Housing System (Stand Alone Rack) for Zebrafish Specifications:  The system should be easiest and least expensive to maintain  Equipped with facilities to get optimal quality water for zebrafish.  Stainless steel/Powder coated aluminum frame.  Water filtration system (Mechanical, Chemical, Biological and flow through UV.  System equipped with automatic/adjustable temperature controller so that desire temperature of water for zebrafish culture achieved.  Autoclavable polycarbonate tanks with lid & baffles 3-4 liters (10-15 No) and 1.5- 2 liters (15-20 No.).  Electrical power in running the system should be compatible with Indian electrical power suppliers.  Brine Shrimp Hatcher with Stand.

Warranty: 5 year comprehensive

20. Zebrafish cardiac activity imaging tracking system Specifications: Complete system (hardware and software) for recording and analysis to investigate live and in a non-invasive way zebrafish embryo and larvae physiological parameters such as cardiovascular activity (heartbeat, blood flow, and the size of the blood vessel), and gut transit under the microscope without fluorescence or other markers.

Warranty: 5 year comprehensive

21. Biochemical Semi-auto Analyzer Specifications:  The system should have Endpoint, kinetic, fixed time and turbidimetric mode.  The system should have tungsten halogen lamp with lamp saver facility.  The aspiration volume should be 250 ul to 500 ul.  Should have complete visual range.  The system should have memory at least 500 samples.  System should have online graphic display of reaction second to second.  System should have previous blank and standard memory facility.

Warranty: 5 year comprehensive

22. Benchtop Multipurpose centrifuge Specifications:  Must have wide temperature range of -10°C- + 40°C  Must have automatic temperature start up at a specific time to automatically pre-prepare temperature before centrifugation  Must be able to maintain at 4°C at max. speed  Max speed of >17,000rpm, max rcf of 30,000g adjustable from 100rpm  Must have digital display of time, speed and temperature.  Programmable time and speed.  Able to switch display between rcf and rpm speed setting.  Separate short spin key.  Automatic locking when lid almost closed.

Warranty: 5 year comprehensive

23. Multi-parameter (pH / Conductivity / TDS/ Salinity / Temperature) tester The instrument should be able to measure wide-range measurements of pH, Conductivity, TDS, Salinity and Temperature. Specifications:  One sensor should measures all parameters without need to switch sensors for different measurements  pH : Range 0.00 to 14.00, Resolution 0.01 pH, Accuracy 0.01 pH, up to 5 point auto calibration and Auto-Buffer Recognition  Conductivity: Range0.0 to 1999μS/cm, Resolution 1μS/cm, Accuracy ±1 % full scale, 3 point auto calibration  TDS: Range 0.0 to 999 ppm, Resolution 0.1 ppm / 1 ppm, Accuracy ±1 % full scale, 3 point calibration  Salinity: Range 0 to 999 ppm, Resolution 0.1 ppm / 1 ppm, Accuracy C % full scale, 3 point calibration  Temperature: Range 0 to 50.0 ºC / 32 to 122.0 ºF, Resolution 0.1 ºC / 0.1 ºF, Accuracy 0.5 ºC / 0.9 ºF.

Warranty: 5 year comprehensive

24. Animal Surgical platform Specifications:  Adjustable temperature control system (Ambient to 45±1 0C).  Continuous temperature monitoring with display.  Shadow and heat free LED lighting system with control knob for optimum illumination  Magnifying glass (4X)  Platform size L 12” x W 14”

Warranty: 5 year comprehensive

25. Tissue Homogenizer Tissue homogenizer suitable for quick processing of small sample quantities and volumes, for pulverizing plant and animal tissue, grain and seed, DNA/RNA extraction. Specifications:  Digital speed control from 2,000 to 30,000rpm  Soft start and LED display  Speed should remain constant even when viscosity changes  Should be easy to handle and clean  Should fit various models of dispersing aggregates  Suitable size stainless steel reusable and synthetic disposable aggregates and other necessary accessories  Should be suitable for handheld or stand mounted operation. Warranty: 5 year comprehensive

Warranty: 5 year comprehensive

26. In vivo Animal Imaging System  Should be fully automatic  Should be able to use image small animals (mice & rats)  The In-Vivo Optical imaging system should be licensed and suitable for fluorescence, bioluminescence (filters ranges up to 400nm to 900nm) and Cerenkov studies for in- vivo and in-vitro.  Complete system inclusive of light tight cabinet, CCD camera, excitation and emission filters, sample stage, computer workstation, optional gas anaesthesia system, other standard and essential accessories, calibration standards, start-up kit, etc. should be supplied. Light Tight Cabinet:  Complete light tight imaging chamber/cabinet  System should have excitation light source for better deep tissue imaging  System should have min of 10 excitation and 4emission filters. Both Excitation and emission filter wheels should be motorized and software controlled  Fitted with LED or transmitted light’s for photographic imaging  Cabinet should be able to accommodate gas anesthesia manifold and its tubing. Essential accessories/attachment should be supplied.  Data generated should be in absolute calibrated according to the National Institute of Standards and Technology (NIST) Specimen Stage:  Stage movement should be software controlled  Stage temperature should be controllable between 20 deg. cel to 40 deg. cel.  Able to accommodate at least 3 nos. of mice at largest FOV. CCD Camera:  High resolution CCD camera grade 1 CCD  Pixel size: 13 x 13microns or better  Thermoelectrically cooled camera- 90oC (absolute)  Quantum efficiency: >80% for 500-700nm range. Computer workstation:  Latest computer configuration compatible with system hardware and software should be provided. Licensed operating system, 22 inch TFT high resolution monitor, 2500 GB HDD, 4 GB RAM, CD /DVD/combo reader/writer, etc. (Provide details of the configuration quoted) Software:  Image acquisition and analysis software should be licensed and suitable for fluorescence, bioluminescence and Cerenkov studies for in-vivo and in-vitro.  Licensed Software package for equipment control should be supplied. Software should also be able to do Absolute calibration, background subtraction and the image math algorithms for producing high-quality, reproducible, quantitative results. Quote optionally for:  Gas anesthesia ports and 5 position manifold with gas flow controller should be supplied. Complete working configuration inclusive of hardware/software, tubing, valves, should be supplied. (optional)  The company should have minimum 10 such installations in India of similar systems. Provide list of current installations (in the last 2-3 years) of the quoted model in India.

Warranty: 5 year comprehensive

27. Gel electrophoresis unit – Horizontal  Horizontal apparatus for separating DNA, RNA and Protein  Should be made of high quality material that is resistance to cleaning agents  UV transparent tray approx 10 (w) x 15 (l) cm  Migration should be uni-directional  Upgradable to gel sizes (approx 18 cm length and 10 cm width)  The tray should accommodate combs for 25 – 30 wells  Electrical cords with male and female connectors  The Comb stand should have positioning pins for height adjustment and metal screws for durability  Buffer requirement approx 400 mL  Gel slicer, niche cleaner, levelling table, gel scoops and casting tray should be included  Suitable Power pack and comb should be provided.

Warranty: 5 year comprehensive

28. Animal bedding disposal station Specifications: Size: approx. 45x30x65(WxDxH)  Cabinet should be stainless steel  Internal Dimension should be 40 x20 (W x D) approx.  Dual long life HEPA filter with 99.99% efficiency for particle size 0.3 microns  Solid work surface cover plate to optionally use the cabinet as a negative work station  Cabinet inside panel should be of stainless steel facilitating easy cleaning

Warranty: 5 year comprehensive

29. Vacuum pump must be compatible with Alpha 2-4 LD Laboratory Freeze Dryer equipment.

Max. pumping speed at 50/60 Hz 5.9 / 6.9 m3/h, Ultimate partial vacuum (abs.) 4 x 10-4 mbar, Ultimate vacuum (abs.) 2 x 10-3 / 1.5 x 10-3 mbar/torr, Ultim. vac. (abs.) with gas ballast 1 x 10-2 / 0.75 x 10-2 mbar/torr, Water vapor tolerance with gas ballast >> 40 mbar, 230 V, 50/60 Hz, 1.6 A. Warranty: 5 year comprehensive

30. Analytical HPLC or UFLC UFLC system Ultra Flow Liquid Chromatographic System for natural product analytical work The system should have the following requirements  Quaternary gradient pump  Wide range of flow rate  Minimum dead volume  The system must be strictly provided with all required modules and accessories Following modules with desired specifications are required A. Quaternary Solvent Delivery System: - Quaternary Pump Should be compatible with In-Line Degasser:4-Channel Vacuum Degasser for effective removal of dissolved gases in chromatography solvents - Pump with necessary accessories viz. solvent cabinet, solvent bottles, tubing, filters, connections etc - Flow Rate Range :- From ~ 0.001 to 10.0 ml/min, with 0.001 ml/ min increments - Operating Pressure :- Equal to or more than 600 bar - Instrument must have hydraulic system comprising of dual piston in series with servo- controlled variable stroke drive, floating pistons and inlet valve B. Manual injector and Auto-sampler: - Option for both auto-sampler and manual (rheodyne) injector should be available - Auto-sampler should have Sample capacity of 100 vials or more - Pressure operating range equal to or more than 600 bar - Should have Injection volume of 0.1–100 μl in 0.1 ul increments - Sample Cooling Thermostat for Auto-sampler required from 4-40 °C - Accessories required must be provided C. Thermostat Column Compartment: - Thermostat with temperature range of ~10oC below ambient to 75oC or more - Column identification accessory should be included - Capacity to hold 3 columns of 30 cm length simultaneously with guard columns - Thermostatted column compartment (TCC) should be equipped with a contact-free column- identification system - TCC must contain all the necessary accessories as tubing, connections, column clip, column identification module etc. D. Detectors (1) Diode-Array Detector –  High Sensitivity Diode array detector  Features like tags for all flow cells and UV lamp to provide new levels of data traceability by recording parameters, cell dimensions, lamp usage, optofluidic waveguides etc  Scanning speed 70 Hz or above  Signal noise should be more than +/-0.6 x 10-6 AU  Should be compatible with 10mm flow cell and 60 mm flow cell for highest sensitivity  Wavelength 190-800 nm (2) UV-Vis Detector  Detection type Double-beam photometer  Number of signals 1  Maximum data rate 80 Hz  Noise more than ± 0.25 x 10-5 AU, at 230 nm*  Drift 0.5 x 10-4 AU/hr, or above at 230 nm

 Linearity more than 2.5 AU upper limit  Wavelength range 190-800 nm  Wavelength accuracy ± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter  Slit width 6.5 nm typical over whole wavelength range (3)Fluorescent Detector  Detection type: Programmable single wavelength fluorescence detector  Light source Xenon flash lamp, lamp life should be 4000 hours or maximum (with extra spare lamp)  Wavelength accuracy ±1 nm (4) RI Detector  Short-term noise ±2.0 x 10-9 RIU or more  Refractive index range 1.00–1.75, calibrated  Flow cell should be more than 5 μL, 5 bar pressure maximum  Temperature control Ambient +5 °C to 55 °C  pH range 2.3–9.5, Automatic zero  Data rate more than 40 Hz (5) ELS Detector. Should be compatible with equipment. E. Chromatography Software:  Compatible data processing unit (Computer) with duplex color laser printer  Licensed Software with a CD and upto date up-gradation of the same  Report making capabilities with built-in calculations, multichromatogram reports etc  Diagnostics/maintenance  Parameter validation management  Compatibility with diode array  Compatibility to import and export the data to other softwares  Ability to acquire and analyses and store spectral library The computer-based control software for the photo diode array detector must have features such as: - Automated peak purity evaluation using peak spectra and peak signal information - Automated spectra library searches - Mathematical operations like normalize, subtract, smooth and derive - Must be able to modify set points and time-programs even during a run - Automatically switch off the lamp at a pre-selected date and time - Syringes 10 µl (2 Nos), 25 µl (2 Nos), 50 µl (2 Nos), 100 µl (2 Nos) 250 µl (2 Nos) - Loops 10, 20, 50, 100 and 250 µl (2 Nos each) - Columns Silica 4.6 x 150 mm 5µ (2 No.), Silica 250 x 4.6 mm 5 µ (2 No.), Silica 10 × 250 mm, 5-7 µm (2Nos), C18 4.6 x 150 mm 5µ (2 Nos), C18 250 x 4.6 mm 5 µ (2 No.), C18 10 × 250 mm, 5-7 µm (2Nos); C8 4.6 x 150 mm 5µ (2 No.), C8 250 x 4.6 mm 5 µ (2 No.), C810 × 250 mm, 5-7 µm (2Nos); Carbohydrate column 4.6 x 150 mm 5µ (1 No.), Carbohydrate column 250 x 4.6 mm 5 µ (1 No.), Amino column 4.6 x 150 mm 5µ (1 No.), amono column 250 x 4.6 mm 5 µ (1 No.), chiral column 250 x 4.6 mm 5 µ (1 No.), Chiral column 4.6 x 150 mm 5µ (1 No.), C18 100e 4.6 x 250 mm 5µ (1 No.), C18, 80, 4.6 x 250 mm 5µ (1 No.); Chiral column 10 × 250 mm, 5-7 µm (1Nos). - Additional Plunger seals (2 Nos.), Seals check valves, cell parts - Peek tubing and fittings, PTFE/ETFE tubing and capillaries - Compression screws (nut and plugs) and ferrules - Tool kit related to Instruments

- Accessories such as guard column, extra ferrules etc. should be provided with the equipment.

Warranty: 5 year comprehensive

31. Flow Cytometer with Sorter: Technical specifications: 1. Machine Type: Should be a bench top model with both the analyser and sorter function and a specific user friendly software for analysis, sorting or instrument setup. 2. Optics: Fibre optics both for Lser and Emission. Excitationa dn Emission optics should be prealigned. Fixed optics should be configurabel up to 13 fluoroscence colours. 3. Lasers: Should have capabilities for 4 prealigned, solid state and air cooled lasers. Required lasers are 488nM (Blue), 375nM (near UV), 635 nM (Red laser). 4. Functional Capacity: a) Should have at least 11 parameters (forward scatter, side scatter and fluoroscent detectors). b) Capability to perform colour compensation/ autocompensation. c) Acquisition speed: More than 100, 000 events per second. d) Temperature controlled sample injection system with flexibility to use microtubes 12x75 mm and 15 ml tubes. e) Multiple sized nozzle tips: 70 micron, 100 micron and 130 micron f) The system should be capable to track the performance and forecast the quality of the components well in advance. 5. Sorter function: a) Both two way and Four way sorting b) Sorting speed > 50,000 events per second c) Automated cell deposition unit for plate sorting/ microwell sorting d) > 98% sorting purity 6. Fluidics cart: Should come sheath, waste, cleaning containers controlled by software. 7. Operating system: a) Windows based platform for both offline and online use b) Computer workstation with latest processor 16 GB memory, 160GB + hard drive, CD-DVD ROM, USB card reader option c) 19” LCD monitors (2 Nos. for instrument control and analysis/ sorting) d) Compatible nertwork colour laser printer (should also be USB compatible) e) Compatible software for instrument control and analysis for both online /offline use. 8. Suitable UPS (at least 30 min backup), high quality strong steel tables, startup kits and post maintenance kits etc. required for installation and smooth running of machine should be included. 9. Should come with a minimum 5 years comprehensive warranty (with spares). 10. A trained technician to be stationed at RMRC, Belagavi to operate the instrument during institutional work hours for five years. Complete training for two institutional personnel to be included free of cost.

32. Ultracentrifuge: General: Tender is invited from reputed original manufacture or their authorized agents/dealers only. Information regarding installation in India and satisfactory service and maintenance may be forwarded with all the details for verification. Technical specifications: - Max. Speed (rpm):100,000 - Max.RCF (xg):803,000 - Speed Control Accuracy: ±2rpm (1,000 rpm to max. speed) - Set Speed Range: 1,000 rpm to max. speed in increments of 100 rpm - Timer from :1 min to 999 hours - Set Temperature Range: from 0ºC to 40ºC with 1ºC increments (Accuracy : +/0.5ºC) - Vacuum System: Rapid vacuum creation facility - Operating Sound: not more than 51dB(A) (running at set speed, under in-house test condition) - Heat Radiation: low - Cooling System: Thermo-module cooling system (CFC/HCFC/HFC-free) - Control Panel: Touch-sensitive color LCD panel (6.5 inch) - Rotor Life Management: Automatic Rotor Life Management for rotors with RLMadapter Registered by serial number to the system for rotors with optical overspeed disk - Single phase, AC230V and 240V +/-10%, Max. 16A (normally 7A) - Touch-sensitive LCD panel - Should have a Visual Balancing by non-contact imbalance sensor - Should be supplied with rotor for 12-15 ml tube - Should be supplied with swing bucket rotor for 40 ml tube for density gradient centrifugation function - Rates for each rotors must be provided separately

Warranty: 5 year comprehensive

33. Confocal Microscope with live cell imaging system: The system should be latest state of the art confocal technology to meet challenging and demanding imaging applications including live cell imaging FRAP, FRET, FCS/ Autocollation, Photo activation/conversion with modular and upgradable option for two laser photon, FLIM and TIRF application in future. Technical specifications: A. Fully motorized confocal laser scanning microscope: 1. Bright field, Flouroscence and DIC observations with accessories for Confocal and head attachment with suitable anti-vibrartion table for the complete system 2. Motorized Z-focus drive with minimum z-step resolution of 15 nm or better with dedicated TFT/LCD control panel/ screen for the control of motorized functions of microscope 3. Auotofocussing facility must be provided 4. 6 position motorized FL filter wheel, 6 position motorized DIC nose piece 5. Motorized programmable X-Y scanning sppecimen stage with universal sample holder and multi well-plate holder 6. 12v/100w hallogen illumination for transmitted light and 120w/130w metal halide or mercury lamp (with at least 1500 hrs or more of lamp life) for flourescence observation with automatic shutter having DC (direct Current) for cnstant and non-fluctuating light 7. High resolution plan apochromat objectives 10x/0.40 or better, 20x/0.80, 40x/1.3 oil, 60/63x/1.40 oil immersion with complete DIC accessories and multi immersion 20/25x (oil/glycerene/ water) objectives for live imaging 8. Fluorescent filters for DAPI, FITC/GFP, Alexa, Cy5 and TRITC/Rhodamine 9. Complete automated and programmable on stage environmental control chamber (incubator) with bigger chamber for long time live cell imaging applications without focus drift with temperature, CO2, Humidity control. The system should be able to use 100% CO2 gas to the chamber to be offered optionally. Facilities for imaging under anarobic conditions should aslo be provided. B. Confocal Scan Head & Detection System: 1. Scan head should have capability of simultaneous detection (best fluorescence signal collection for imaging of even weakly bonded dyes) and separation of at least 4 or higher Flurochromes with independent spectral PMT detectors. Scan head with future upgradability/capability for more simultaneous fluorochromes detection/separation would be given preference. 2. The detection unit should have capability of conventional intensity based Confocal Imaging as well as Spectral Confocal Imaging for all channels. 3. All the detectors of the scan head should be filter free and freely selectable emission bandwidth detection capability to suit to the emission spectra of the all dyes. 4. Equipped with either spectral detector having multiple gratings options or with prism based spectral dispersion including Acoustic beam splitter for enhanced/ improved emission signal. 5. All the detectors should be capable of spectral detection with freely selectable spectral range with at least spectral resolution of 10 nm or better. 6. Laser scan optics should cover the entire wavelength range from 350 to 1100 nm and should have simultaneous laser coupling ports for UV, Visible and IR lasers. 7. Technology should be latest & fast & capable to avoid any photobleachingor phototoxicity of the sample. 8. Computer controlled continuously variable single pinhole system to cover more area for higher brightness and without affecting sectioning performance. 9. High speed XY galvo-scanner with at least 180 or higher degree scan rotation with total scan flexibilities of Line, free hand curved line and different axis combinations.

10. The laser scanner should have dual scan capability of fast ROI scan for bleaching/ and normal scan for Imaging, of any irregular shape to conduct experiments like FRAP, FLIP, photo activation, photo conversion and photo bleaching. The system that doesn’t have such dual scan capability should include a separate laser scanner for bleaching, photo- activation and photo conversion with appropriate upgradable software. 11. Maximum scan resolution of up to 4Kx4K with a scan field of 20mm or higher and should be selected freely down to 4x4 pixels. 12. Scan zoom of 1-40x or more continuous variable. Multistep scanning zoom will be preferred. 13. System should be capable of acquiring minimum 5-10 fps in both channel and spectral mode at 512x512 pixel resolution and should increase with ROI and zoom selection to 200fps or better Digitization capability of 8/12/16 bit should be available with the system. 14. An additional transmitted light detector should be there for bright field and DIC imaging. 15. Laser module with various wavelength covering the range of 405 nm to IR range (>1024 nm) to be included. All the visible and UV laser lines should be controlled through AOTF for fast laser attenuation and switching in synchronization with scanner. Solid-state lasers and pulse lasers at higher wavelengths is preferred. Accessories for polarized laser may be included. 16. Focus drifts control mechanism/ stability manager (Preferably laser based) for long term time lapse imaging application to be offered.

C. System Control and Imaging Software: 1. Software (With latest controlled computer) should be capable of controling motorized functions of microscope, digital camera, scan head control and image aquisition/ processing. 2. Software module (stand-alone version may be included) for co-localization analysis and volume rendering 3D/$D image re-construction, spectral imaging/ emmission fingerprinting, multiple point time lapse, imaging and analysis for FRAP, FRET, photo activation/ conversion and photobleaching. 3. Should have Line, curved line, frame, Z-stack, Time series imaging capabilities. 4. Additional offline analysis software of similar version should be provided.

D. Additional Requirements: 1. Equipment should come with five years comprhensive warranty for all components of the equipment and accessories, including UPS. 2. Trained personnel for handling the equipment should be provided for five years with no additional cost. 3. Both on site and off site training for minimum of TWO RMRC staff shopuld be provided by the company. 4. Free installation requirement including UPS and site preparation to be done by the company. 5. Product catalogue and public website of the manufacturer to be provided to support all claims. 6. Documented recommendations for service complience from users in Southern India should be provided.

34. Inverted Fluorescence Microscope: General: Tender is invited from reputed original manufacture or their authorized agents/dealers only. Information regarding installation in India and satisfactory service and maintenance may be forwarded with all the details for verification. Inverted Fluorescence Microscope suitable for observation under bright field, Phase Contrast and Epifluorescent attachment preferably for both glass and plastic containers. Technical specifications: Objectives: Objectives 10x, 20x, 40x and 60-63X, where 40X and 60X to be long distance lens type. Objectives should be suitable for all purpose requirements. Eyepiece tube: Trinocular tube with camera attachment. Eyepiece lens: 10X with field of view 22~23 mm. Nosepiece: Quintuple (5) nosepiece or higher Stage. Moveable stage: Hard anodized surface with standard size, objective guide and universal mounting frame. Light source: LED type only; preferable with reflector turret. Filters: Two or more position filter slider; Filters for UV (DAPI, Hoechxt, Alexa Fluor) Blue (GFP, FITC, Cy2), Green (RFP, Rhodamine, TRITC, Cy3, PI) to be included. Filters to be interchangeable by just pull and push without use of any tool. Focusing: Facility for coarse and fine focusing Photographic attachments: High resolution CCD color microscopy camera (with 5 megapixel or more,and supported licensed software); interactive measurement of morphologic parameters with u bar and text annotation;and photography and microscopic image analysis.Suitable PC along with UPS compatible to support simultaneous usage of PC and the microscope to be supplied. Operating voltage 220 Volts.

Warranty: Warranty: 5 year comprehensive

35. Multimode Microplate Reader and Washer: General: Tender is invited from reputed original manufacture or their authorized agents/dealers only. Information regarding installation in India and satisfactory service and maintenance may be forwarded with all the details for verification. Technical specifications: A. Elisa Reader: Detection Mode: Absorbance Light Source: LED Photo detector: Photo diode Plate type: 96 well microplate including strip well microplate. Measurement mode: Endpoint, kinetic and multi-label measurements Measurement Channel: 8 channel Plate Shaking mode Measurement range: 0-4 OD Resolution: 0.001 OD or better Wavelength Range: 400-750nm Wavelength selection up to 8 filters Standard filters 405nm, 450nm, 492nm, 620nm. Accuracy: 0.010 OD or better Precision: 0.005 OD or better Linearity: ≤ 1 % or better Full qualitative and quantitative analysis including all major curve fittings andcomprehensiveQC function. Windows based software Display: Operation through PC: i3 processor 4GB RAM, 500GB HDD, 18.5” TFT, Win10 ect, b/w laser printer, UPS (capability to work as standalone or with P.C. Connectivity.

B. Elisa Plate Washer Cross wise aspiration, over flow washing, bottom washing 16channel manifold (8 channel × 2) Manifold, plate carrier auto cleavable and also can be sterilized 1-4 Wash liquid channel Equipment should be un-pressurized, capable of using any bottle or container. Volume of wash liquid dispensed 50 to 3000μl with 50μl increment Suitable for U,V& flat bottom micro plate etc Residual volume per well <2 μl

Warranty: 5 year comprehensive

36. Real Time RT-PCR: Tender is invited from reputed original manufacture or their authorized agents/dealers only. Information regarding installation in India and satisfactory service and maintenance may be forwarded with all the details for verification.

Technical specifications: 1. Table top model 2. Complete system including basic system, essential accessories, state-of-art computer workstation, acquisition and analysis software, start up kit including calibration standards etc. 3. Open system to accommodate TaqMan, SYBR green and all other fluorescent dye based chemistries 4. Pettier based 96 well block 5. Standard optical 96 well plates, 0.2 ml strips, 0.2 ml tubes compatibility 6. Main sample value requirement- 5µl 7. CCD camera with halogen/LED and at least five excitation and five emission filters 8. Multiplexing ability up-to five dyes in a single run 9. Calibrated dyes at installation: FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5, should offer flexibility in dye selection 10. Facility to calibrate new dye within the wavelength range without addition of new filters 11. Passive reference dye ROX or any other calibrated dye and should be optional 12. Option for melt curve analysis 13. Temperature range 4°C to 100°C 14. Sensitivity: Detection of 1 copy of template 15. Software applications: Comparative Ct, Standard Curve, Relative Standard Curve, Allelic Discrimination/SNP Genotyping, Plus/Minus, Dissociation/ melt Curve 16. 220 V/50 Hz All accessories CE mark or equivalent.

Warranty: 5 year comprehensive

37. CO2 Incubator 28° C: Incubator can be set at 28º C but without CO2. The cells can be grown with a specialised media which don’t require CO2 for Dengue culture in C6/36 cells. Technical specifications: - Should have an Interior volume of 100-150 liters - Should be a Direct Heat air Jacketed with uniform temperature circulation - Temperature control range: ambient to 28ºC - Temperature control uniformity : ±0.25ºC - CO2 control range: 0% to 20%. ±0.15% in 25ºC ambient, setting 28ºC - CO2 calibration: Automatic, continuous zero reference calibration, optional semi-automatic one point gas calibration system. - Interior humidity: 95% RH at 28ºC by natural evaporation with humidifying pan with reflective optional low water sensor - Temperature and CO2 control : P.I.D control system set point resolution 0.1ºC,0.1% - Display: Alphanumeric LCD digital display - Should have a uniquely designed trays without any additional clamping and screws for additional storagespace andto avoid cumbersome cleaning procedure. - Insulation: Rigid foam poly urethane - Outer door: Reversible and Heated. - Should have a programmable ultraviolet lamp, isolated from cell cultures, thatsterilizes conditioned air and humidity reservoir water to prevent contaminationwithout effecting cell cultures in vitro

Warranty: 5 year comprehensive

38. CO2 Incubator 37° C: General: Tender is invited from reputed original manufactureor their authorized agents/dealers only.Information regarding installation in India and satisfactory service and maintenance may beforwarded with all the details for verification. Technical specifications:  Should have an Interior volume of 100-150 liters  Should be a Direct Heat air Jacketed with uniform temperature circulation  Temperature control range: ambient to 37ºC  Temperature control uniformity : ±0.25ºC  CO2 control range: 0% to 20%. ±0.15% in 25 ºC ambient, setting 37 ºC  CO2 calibration: Automatic, continuous zero reference calibration, optional semiautomatic one point gas calibration system.  Interior humidity: 95% RH at 37ºC by natural evaporation with humidifying pan with reflective optional low water sensor  Temperature and CO2 control : P.I.D control system set point resolution 0.1ºC,0.1%  Display: Alphanumeric LCD digital display  Should have a uniquely designed trays without any additional clamping and screws for additional storagespace andto avoid cumbersome cleaning procedure.  Insulation: Rigid foam poly urethane  Outer door: Reversible and Heated.  Should have a programmable ultraviolet lamp, isolated from cell cultures, thatsterilizes conditioned air and humidity reservoir water to prevent contaminationwithout effecting cell cultures in vitro.

Warranty: 5 year comprehensive

39. Dissecting Microscope: General: Tender is invited from reputed original manufacture or their authorized agents/dealers only. Information regarding installation in India and satisfactory service and maintenance may be forwarded with all the details for verification. Technical specifications: - Should have paired adjustable wide-field eye piece: 10x (field no. 23mm) with dioptre ring for focussing through both eyes. - With turret mounted inbuilt objectives (2x and 4x) - Should have fine focussing control knobs - Zoom control should be provided - Working distance should be at least 105mm - Should have a stage plate with clips to hold glass slide in place. - There should be an inbuilt light stand (incident and transmitted illumination). - Light/ power switch and dimmer for adjusting desired level of illumination for incident illumination should be provided. - The microscope should have a solid metallic body with sturdy stand - Microscope should be CE/FDA/BIS certified. - Mandatory for the Bidders to enclose proper Brochures from the Respective Principles along with the WEB link for their Global Website.

Warranty: 5 year comprehensive

40. Analytical Micro Balance:

General: Tender is invited from reputed original manufacture or their authorized agents/dealers only. Information regarding installation in India and satisfactory service and maintenance may be forwarded with all the details for verification. Technical specifications: - Capacity: 220 gm, - Readability: 0.01 mg, - Repeatability (+/-):0.03mg - Viewing withA.E.P, RS232 Interface, Hanger for below balanceweighing - Automatic External Calibration Conforms GLP/GMP and ISO 9001.

Warranty: 5 year comprehensive

41. Adjustable volume Single Channel Pipettes [Range 0.2-2μl; 2-20μl; 20-200μl; 100- 1000μl]:

Technical specifications: Must be of a reputed brand, from manufacturer/authorized dealers having calibration facility in Karnataka. Necessary evidence to be provided. 1. ISO8655 certified multichannel pipettes of variable volume compatible with universal tips. 2. Totally adjustable, air-displacement pipette. 3. Usable for dispensing of aqueous fluids of moderate viscosity. 4. As the parts of pipette may be exposed to chemicals, so it should have provisions for autoclaving. 5. Long self-life and high resistance to aggressive chemicals. 6. Permissible volume errors should be minimum.

Range Increment Accuracy Precision 0.2 to 2.0 μL 0.01 μL ±12.0 to 2.5% 10.0 to 2.0% 2 to 20 μL 0.10 μL ±3.0 to 1.0% 2.5 to 0.4% 20 to 200 μL 1.0 μL ±1.8 to 0.6% 0.7 to 0.2% 100 to 1000 μL 5.0 μL ±1.0 to 0.6% 1.6 to 0.2%

Warranty: 5 year comprehensive

All instruments must work at 220 V supply. Any other supply needs to be suitably compensated with appropriate transformer, stabilizer etc.

ANNEXURE-III

TERMS AND CONDITIONS TO SUBMIT QUOTATION FOR SUPPLY OF EQUIPMENTS

IMP. Note: PLEASE READ CAREFULLY these Terms &Conditions and copy of the same, duly signed by the supplier must accompany the Technical Quotation. Otherwise the quotation will not be considered.

1. EMD Envelope: EMD Draft / Bankers guarantee should be sealed in separate envelope (along with the exemption certificate, if applicable). Mark this as Envelope I

2. Technical Bid Envelope: This should be sealed in separate envelope marked as Envelope II indicating on the cover Reference No. and Equipment name. This envelope should contain list of users of the product quoted along with adequate technical literature. No indications pertaining to price or commercial terms may be made in this envelope. It should also carry complete specification, warranty and AMC particulars as required by us.

3. Commercial Bid Envelope: This should be in separate envelope marked as Envelope III indicating on the cover Reference No. and product name. Cost of the equipments should be mentioned clearly in the commercial offer only. The optional and any other essential items/accessories required for the maintenance of the facility for the next three years should also be specified separately. Packing, Forwarding, Freight & Insurance and Commissioning Charges, if any extra, must be quoted.

4. All these envelopes I, II and III should be sealed inside and super scribed in a separate cover with the words as under:

“Quotation for ______”

Enquiry No. ………………………………………. Due Date…………………………

Please use separate packets for each equipment quoted

5. Your offer must be kept open up to 31.03.2017.

6. Specific Information should be furnished as to whether the items have been offered Ex- stock or will have to be imported. Delivery period should be clearly indicated in the quotations. 7. The rates of Sales Tax, G.S.T, C.S.T and Excise Duty must be clearly indicated. Wherever chargeable S.T/ G.S.T / C.S.T Numbers must be indicated. It will be deemed that No Taxes are payable by us if such items are not indicated.

8. A Performance Bank Guarantee equivalent to 10% of the cost of the equipment, is compulsorily, required for 3 years from the principals or their counter parts or their Agents in India commencing from the date of satisfactory installation (copy of the format is enclosed), is to be provided upon placement of purchase order.

9. The supply of spare parts is to be guaranteed at least for a period of 10 years after the supply of the equipment.

10. The name of the manufacturer must be clearly specified while indicating the make /brand and name and address of the manufacturer. Country of origin, country of shipment and currency in which rates are quoted are to be mentioned. Supplies are to be made in the manufacturer’s original packing wherever possible.

11. If similar or identical equipment was supplied to other ICMR Labs. / Institutes, the details of such equipment for the preceding three years may also be given together with the price eventually or finally paid.

12. The name of the Indian Institution(s) where is the requisite Equipment has been supplied may also be given along with full postal Address / Telephone / Fax /E- mail / Address of the users.

13. Rates quoted by outstation firms should be on F. O. R ICMR - NITM, Belagavi, basis only and the rates quoted by local Firms should be on free delivery basis at ICMR – National Institute of Traditional Medicine (NITM), Belagavi 590010

14. The Supplier is required to furnish the Permanent Account Number (PAN) allotted by the Income Tax Department. If registered with the National Small Industries Corporation, the registration number, purpose of registration and the validity period of registration etc. should also be provided along with Quotation.

15. The Supplier has to state in detail the Electrical Power / UPS requirements, floor Space, headroom, foundation needed and also to state whether Air conditioned environment is needed to house the equipment etc. and to run the tests. All essential accessories needed must be quoted in addition to the main equipment otherwise the offer will not be considered.

16. Please note that all the Bank Charges inside and outside India, including opening of LC, communication, confirmation, amendments etc., if any, will be borne by Beneficiary’s.

17. (A) In case of Indigenous Equipments, the offer should contain the Basic Price and percentage of Excise Duty should be shown separately, since ICMR - NITM, Belagavi is exempted from payment of Excise duty vide Govt. Notification No. 10 / 97-Central Excise dated 1st March, 1997.

(B) ICMR - NITM is exempted from payment of Custom Duty vide Govt. Notification No.51 / 96- Customs dated 23rdJuly, 1996. Since the suppliers are requested to quote only on FOR basis, custom duty will not be applicable for the suppliers.

18. The Director, the ICMR – National Institute of Traditional Medicine (NITM), Belagavi does not bind himself to accept only the Lowest bidder. He reserves the right to accept or to reject any or all quotations without assigning any reason.

19. No interim queries will be entertained.

20. The payment of bill for Goods / Equipments supplied will be subject to inspections of Goods / Equipments by competent authority.

21. Payment will be made by crossed cheques /DD on Canara Bank, Khade Bazar Belagavi, after receipt of equipment by the stores in satisfactory conditions in full. In case of foreign manufactures, the necessary LC will be opened.

22. In case of Supply Orders, the dates must be strictly adhered to. In case of delayed supplies without prior approval of this Centre, the Director, ICMR - NITM reserves the right to reject /refuse the items in part or in full at the discretion of the Director. The Director also reserves the right to claim liquidated damages for non- delivery of the items in time. 23. Demurrage charges arising out of delay / fault on part of suppliers must be borne by the suppliers. 24. INSTALLATION: The Equipments are required to be installed at the ICMR – National Institute of Traditional Medicine (NITM), Nehru Nagar, Belagavi 590010, Karnataka and Compulsory three years’ comprehensive warranty and two years’ non-comprehensive warranty is to be given from the date of Installation. Subsequently Training is to be provided to the concerned scientists / officials at suppliers cost. The supplier shall be responsible for installation, commissioning, demonstration, training etc. and for after sales service during the warranty and thereafter. 25. In case the equipments that require stabilizers, or any compulsory accessory, company has to provide the same along with the equipment. 26. The firm should provide non-comprehensive maintenance from 4th year to 10th year at 1.5% of the basic cost of the instrument with an increment of 10% subsequent i.e from 11th year onwards. Deviation, if any is to be clearly mentioned in commercial bid. 27. The firm should provide discount of 20% on the list price of all spares parts up to 10years. 28. ICMR-NITM will not be responsible: a) For delayed / late quotations submitted/ sent by Post/ Courier etc. b) For submission/ delivery of quotations at wrong places other than the Office of the ICMR-NITM, Belagavi. 29. (A) Quotations without following the Two-Bid Offer System, (B) Late/Unsigned/incomplete Quotation, (C) Conditional Offers, (D) Quotation submitted by Fax / E-mail /Telegraphic / Telex, will summarily be rejected. 30. ICMR - NITM will not provide any accommodation/transportation for the Engineers / Representatives for attending Installation, Commissioning, Demonstration, training etc. It is the absolute responsibility of the Principal Supplier/ Indian Agent to make their own arrangements.

Annexure-IV

PROFORMA FOR BANK GUARANTEE FOR PERFORMANCE BOND/SECURITY DEPOSIT (To be stamped in accordance with Stamp Act)

(STAMP PAPER SHOULD BE PURCHASED IN THE NAME OF ISSUING BANK)

Ref:

Dated: ______

Bank Guarantee No.

To, The Director, ICMR – National Institute of Traditional Medicine (NITM) Opp: KLE Hospital, Nehru Nagar, Belagavi- 590010.

Dear Sir,

1. In consideration of ICMR – National Institute of Traditional Medicine (NITM) (here in after referred to as ICMR - NITM which expression shall unless repugnant to the context or meaning thereof, includes all its successors, administrators, executors and assignees) having its head / registered office at here in after referred to as ‘the Contractor’ which expression unless repugnant to the context or meaning thereof, include all its successor, administrators, executors and assignees ) and ICMR - NITM having agreed that the Contractor shall furnish to ICMR - NITM, for (scope of work)______the faithfully performance of the entire contract.

2. We (Name of Bank)______registered under law of having head / registered office at (here in after referred to as “the Bank” which expression shall unless repugnant to the context or meaning thereof, include all its successors, administrators, executors and permitted assignees) do hereby guarantee and undertake to pay immediately on first demand in writing and any/all monies to the extent of Indian Rs.______(in figures) Indian Rs.______(in words) Without any demur, reservation, Contractor. Any such demand by ICMR - NITM on the Bank by serving a written notice shall be conclusive and binding without any proof on the bank as regards the amount due and payable not withstanding any dispute(s) pending before any court, Tribunal, Arbitrator, or any other authority and/or any other matter or thing what so ever as liability under these presents being absolute and unequivocal.

We agree that the Guarantee here in shall be irrevocable and shall continue to be enforceable until it is discharged by ICMR - NITM in writing. The guarantee shall not be determined, discharged or affected by the liquidation, winding up, dissolution or insolvency of the Contractor and shall remain valid binding and operative against the bank.

3. The Bank also agree to that ICMR - NITM at its option shall be entitled to enforce this guarantee against the Bank as a principal debtor, in the first instance, without proceeding against the Contractor and not withstanding any security or other guarantee that Institute may have in relation to the Contractor’s liabilities.

4. The Bank further agree that ICMR - NITM shall have the fullest liberty without our consent and without affecting in any manner our obligations here in under to vary, any of the terms and conditions of the said contractor to extend time of performance by the said Contractor(s) from time to time to postpone for any time or from time to time exercise of any of the powers vested in ICMR - NITM against the said Contract(s) and to forebear or in force any of the terms and conditions relating to the said agreement and we shall not be relieved from our liability by reason of any such variation, or extension being granted to the said Contractor(s) or for any such matter or thing what so ever which under the law relating to sureties would, but for this provision, have effect of so relieving us.

5. The Bank further agrees that the guarantee here in contained shall remain in full force during the period that is taken for the performance of the Contract and all the dues of ICMR - NITM under or by virtue of the contract have been fully paid and its claim satisfied or discharged or till RMRC discharge this guarantee in writing, whichever is earlier.

6. This Guarantee shall not be discharged by any change in our constitution, in the constitution of RMRC or that of the Contractor.

7. The Bank confirms that this guarantee has been issued with observance of appropriate laws of the country of issue.

8. The Bank also agree that this guarantee shall be governed and construed in accordance with Indian Laws and subject to the exclusive jurisdiction of Indian Court of the Place from where tender have been invited.

9. Not with standing anything-contained herein above, our liability under this guarantee is limited to India Rs. (in figures) (in words) and our guarantee shall remain in force until (indicate the date of expiry).

Any claim under this Guarantee, must be received by us before the expiry of this Guarantee. If no such claim has been received by us by the said dated, the rights of ICMR - NITM under this Guarantee will cease. However, if such claim has been received by us within the said date, all the rights of ICMR - NITM under this Guarantee shall be valid and shall not cease until and we have satisfied that claim.

In witness where of, the bank through its authorized officer has set its hand and stamp on this day of ______20 .

Witness 1

(Signature) (Signature) Full Name, Designation Full Name, Designation Official Address Official Address with Stamp

Attorney As Power of Attorney No……………………………….

Date…………………………

Witness No.2

(Signature) Full Name and Official Address (in legible letters)

Note: This Bank Guarantee/all further communication relating to the Bank Guarantee should be forwarded to: The Director, ICMR – National Institute of Traditional Medicine (NITM), Nehru Nagar, Belagavi 590010, Karnataka.