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AK3L1 Polyclonal antibody Catalog Number:13206-1-AP Featured Product 1 Publications www.ptglab.com

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 13206-1-AP BC040224 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 513 μg/ml by 205 WB 1:500-1:3600 Bradford method using BSA as the Full Name: IP 0.5-4.0 ug for IP and 1:200-1:1000 standard; 3-like 1 for WB Source: IHC 1:50-1:500 Calculated MW: IF 1:50-1:500 Rabbit 223 aa, 25 kDa Isotype: Observed MW: IgG 30 kDa Immunogen Catalog Number: AG3966

Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : MCF7 cells, human liver tissue Cited Applications: IP : MCF-7 cells, WB IHC : human breast cancer tissue, Species Specificity: human, mouse IF : MCF-7 cells, Cited Species: human Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It Background Information catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the and pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

Notable Publications Author Pubmed ID Journal Application Chunbao Zang 30479565 Cancer Cell Int WB

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

MCF-7 cells were subjected to SDS PAGE followed Immunohistochemical analysis of paraffin- Immunohistochemical analysis of paraffin- by western blot with 13206-1-AP (AK3L1 antibody) embedded human breast cancer tissue slide using embedded human breast cancer tissue slide using at dilution of 1:400 incubated at room temperature 13206-1-AP (AK3L1 antibody) at dilution of 1:200 13206-1-AP (AK3L1 antibody) at dilution of 1:200 for 1.5 hours. (under 10x lens. Heat mediated antigen retrieval (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). with Tris-EDTA buffer (pH 9.0).

IP result of anti-AK3L1 (IP:13206-1-AP, 4ug; Immunofluorescent analysis of (4% PFA) fixed Detection:13206-1-AP 1:300) with MCF-7 cells MCF-7 cells using 13206-1-AP (AK3L1 antibody) at lysate 3200 ug. dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).