EUROPE MOUSE SOPF EUROPEAN Charles River Laboratories France Location Health Status CRL France C03 Mice SOPF Most Recent Past 18 Months Summary Item Method Primary Lab Test Date Positive / Tested Positive / Tested Viruses Minute Virus of Mouse (MVM) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 95 Mouse Parvovirus (MPV) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 95 Mouse Virus (MHV) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Mouse Norovirus (MNV) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Theiler Virus (TMEV - GDVII) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Rotavirus (EDIM) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Sendai (SEND) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Reovirus 3 (REO3) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Virus (PVM) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Mouse Thymic Virus (MTV) ae MFIA RADS US ------Mouse Cytomegalovirus (MCMV) ae MFIA RADS US ------Hantaan Virus (HANT) ae MFIA RADS US ------Lymphocytic choriomeningitis (LCMV) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Mouse Adenovirus (MAV 1&2) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 K Virus (K) ae MFIA RADS US ------Ectromelia (ECTRO) ae MFIA RADS US 03-Jan-2019 0 / 2 0 / 4 Polyoma Virus (POLY) ae MFIA RADS US ------Lactate Dehydrogenase Virus (LDV) ae MFIA RADS US ------ Tyzzer's Disease (CPIL) ae Necropsy Exam RADS EU ------Bordetella bronchiseptica ae Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 Citrobacter rodentium ae Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 Corynebacterium kutscheri ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 Corynebacterium bovis af PCR RADS EU 03-Jan-2019 0 / 2 0 / 4 Mycoplasma pulmonis ae MFIA RADS EU 03-Jan-2019 0 / 2 0 / 4 Pasteurella pneumotropica ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 ad Culture RADS EU ------Salmonella spp ae Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 Streptobacillus moniliformis ae Direct Exam RADS EU 03-Jan-2019 0 / 2 0 / 4 Beta Hemolytic Streptococci ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 Streptococcus pneumoniae ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 4 hepaticus ae PCR RADS US 03-Jan-2019 0 / 2 0 / 4 Helicobacter bilis ae PCR RADS US 03-Jan-2019 0 / 2 0 / 4 Helicobacter, other spp ae PCR RADS US 03-Jan-2019 0 / 2 0 / 4 Staphylococcus aureus ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 5 ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 5 ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 5 ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 5

Page 1 of 2 Reported on Friday, January 18, 2019 at 6:21 GMT EUROPE MOUSE SOPF EUROPEAN Charles River Laboratories France Location Health Status CRL France C03 Mice SOPF Most Recent Past 18 Months Summary Item Method Primary Lab Test Date Positive / Tested Positive / Tested Bacteria Pneumocystis spp. af PCR PCR 03-Jan-2019 0 / 2 0 / 4 ad Culture RADS EU 03-Jan-2019 0 / 2 0 / 5 CAR Bacillus ae MFIA RADS EU 03-Jan-2019 0 / 2 0 / 4 Lesions observed Gross Exam ae Clinical exam and necropsyRADS EU ------Parasites Ectoparasites ae Direct Exam RADS EU 03-Jan-2019 0 / 2 0 / 4 Helminths ae Direct Exam RADS EU 03-Jan-2019 0 / 2 0 / 4 Enteric Pathogenic Protozoa ae Direct Exam RADS EU 03-Jan-2019 0 / 2 0 / 4 Other protozoa ae Exam EU RADS 03-Jan-2019 0 / 2 0 / 4 E. cuniculi ae MFIA RADS US ------* dashes indicate not tested during specified period. HOUSED STRAINS Outbred Inbred Hybrid Other Strains NMRI C3H OT I Congenic SWISS NUDE C57BL/6J CB17SCID Legend: RADS EU = Research Animal Diagnostic Services Europe Lyon, France RADS US = Research Animal Diagnostic Services United States Wilmington Massachusetts

Primary laboratory for Necropsy Exam, Direct Exam, and Culture is RADS EU Primary laboratory for MFIA and PCR is RADS US

COLONY POLICY FOR POSITIVE RESULT: a = immediate termination; b = planned future recycle of the colony; c = no action.

TESTING SCHEDULE: d = screened every six weeks; e = screened quarterly on 1/4 of isolators; f = screened annually

C3 CRLF Mice SOPF

Page 2 of 2 Reported on Friday, January 18, 2019 at 6:21 GMT