Moore et al. Microb Cell Fact (2021) 20:159 https://doi.org/10.1186/s12934-021-01632-0 Microbial Cell Factories

CORRECTION Open Access Correction to: Refactoring of a synthetic raspberry ketone pathway with EcoFlex Simon J. Moore1,3,5, Yonek B. Hleba1,3, Sarah Bischof5, David Bell1,3, Karen M. Polizzi1,2,4* and Paul S. Freemont1,3,6,7*

Correction to: Microb Cell Fact (2021) 20:116 Author details 1 https://doi.​ org/​ 10.​ 1186/​ s12934-​ 021–01604-4​ Centre for Synthetic Biology and Innovation, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK. 2 Depart- Following publication of the original article [1], the ment of Life Sciences, Imperial College London, South Kensington Campus, authors identifed an error in Fig. 1b. Exhibition Road, London SW7 2AZ, UK. 3 Department Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College Te correct Fig. 1b and its caption is given in this erra- London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK. tum. Te original article has been revised. 4 Department of Chemical Engineering, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK. 5 Present Address: School of Biosciences, University of Kent, CT2 7NJ Canterbury, England. 6 The London Biofoundry, Imperial College Translation & Innovation Hub, White City Campus, 80 Wood Lane, London W12 0BZ, UK. 7 Dementia Research Institute Care Research and Technology Centre, Imperial College London, Hammer- smith Campus, Du Cane Road, London W12 0NN, UK.

The original article can be found online at https://​doi.​org/​10.​1186/​s12934-​ 021-​01604-4. *Correspondence: [email protected]; [email protected] 1 Centre for Synthetic Biology and Innovation, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK Full list of author information is available at the end of the article

© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat​ iveco​ mmons.​ org/​ licen​ ses/​ by/4.​ 0/​ . The Creative Commons Public Domain Dedication waiver (http://creat​ iveco​ ​ mmons.org/​ publi​ cdoma​ in/​ zero/1.​ 0/​ ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Moore et al. Microb Cell Fact (2021) 20:159 Page 2 of 2

L-Tyrosine p-coumarate p-coumaroyl-CoA HBA Raspberry ketone NAD(P)H + H+ OH OH OH OH OH NAD(P)+ ATP 3 NH3 CoA AMP 0.03 s-1 A O 1 O 2 O O 5 O -1 -1 -1 0.4 s 2.3 s 2CO2 0.4 s CoA 2CoA-SH OH OH S O O - NH2 O S-CoA

AMP 4 1 TAL - Tyrosine ammonia lyase Pathway key -1 ATP 18 s 2 PCL - p-coumaroyl-CoA synthetase CoA k O O 3 BAS - Benzalacetone synthase cat Fast Slow - - 4 MatB - Malonyl-CoA synthetase OO 5 RKS- Raspberry ketone reductase

Raspberry ketone 100 HBA p-coumarate B L-tyrosine

M) 75 µ (

Key 50 Intermediate

J23114 25 J23100 0 1 tal No gene 2 pcl 3 bas 4 matB 5 rks

Pathway Combination Fig. 1 A comparison of raspberry ketone biosynthesis in vitro and in vivo identifes pathway limitations. A Biosynthetic pathway for raspberry

ketone and Kcat values are referenced within the main text. Intermediates quantifed throughout LC–MS include L-tyrosine (white box), p-coumarate (yellow box), HBA (orange box) and raspberry ketone (red box). B EcoFlex refactoring of the raspberry ketone pathway. See methods for details of growth conditions. Data and error bars (standard deviation) is representative of three biological repeats

Reference Publisher’s Note 1. Moore SJ, Hleba YB, Bischof S, Bell D, Polizzi KM, Freemont PS. Refactoring Springer Nature remains neutral with regard to jurisdictional claims in pub- of a synthetic raspberry ketone pathway with EcoFlex. Microb Cell Fact. lished maps and institutional afliations. 2021;20:116. https://​doi.​org/​10.​1186/​s12934-​021-​01604-4.