CASP3 & DFFA Protein Protein Interaction Antibody Pair

Catalog # : DI0148 規格 : [ 1 Set ] List All

Specification Application Image

Product This protein protein interaction antibody pair set comes with two In situ Proximity Ligation Assay (Cell) Description: antibodies to detect the protein-protein interaction, one against the CASP3 protein, and the other against the DFFA protein for use in in situ Proximity Ligation Assay. See Publication Reference below.

Reactivity: Human

Quality Control Protein protein interaction immunofluorescence result. Testing:

Representative image of Proximity Ligation Assay of protein-protein interactions between CASP3 and DFFA. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal antibody 1:1200 and anti-DFFA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

Supplied Antibody pair set content: Product: 1. CASP3 rabbit purified polyclonal antibody (20 ug) 2. DFFA mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols.

Storage Store reagents of the antibody pair set at -20°C or lower. Please aliquot Instruction: to avoid repeated freeze thaw cycle. Reagents should be returned to - Page 1 of 4 2016/5/19 20°C storage immediately after use.

MSDS: Download

Publication Reference 1. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY. Mol Cell Proteomics. 2013 Feb 8. [Epub ahead of print]

Applications In situ Proximity Ligation Assay (Cell)

CASP3 DFFA

Gene Information

Entrez GeneID: 836

Gene Name: CASP3

Gene Alias: CPP32,CPP32B,SCA-1

Gene caspase 3, apoptosis-related cysteine peptidase Description:

Omim ID: 600636

Gene Ontology: Hyperlink

Gene Summary: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq

Other OTTHUMP00000165054,PARP cleavage protease,SREBP cleavage Designations: activity 1,Yama,apopain,caspase 3,caspase 3, apoptosis-related cysteine protease,cysteine protease CPP32,procaspase3

Gene Information

Entrez GeneID: 1676

Gene Name: DFFA

Gene Alias: DFF-45,DFF1,ICAD

Gene DNA fragmentation factor, 45kDa, alpha polypeptide Description:

Omim ID: 601882

Gene Ontology: Hyperlink

Gene Summary: Apoptosis is a cell death process that removes toxic and/or useless Page 2 of 4 2016/5/19 cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq

Other DFF45,DNA fragmentation factor, 45 kD, alpha polypeptide,DNA Designations: fragmentation factor, 45 kD, alpha subunit,OTTHUMP00000001903,OTTHUMP00000001904

Interactome 1

Interactome 2

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