Autophagy Governs Pro-tumorigenic Effects of Mitotic Slippage-induced Senescence

Rekha Jakhar, Monique N.H. Luijten, Alex X.F. Wong, Bing Cheng, Ke Guo, Suat P. Neo, Bijin Au,

Madhura Kulkarni, Kah J. Lim, Jiamila Maimaiti, Han C. Chong, Elaine H. Lim, Tee B. K. Tan, Kong W.

Ong, Yirong Sim, Jill S. L. Wong, James B.K. Khoo, Juliana T.S. Ho, Boon T. Chua, Indrajit Sinha,

Xiaomeng Wang, John E. Connolly, Jayantha Gunaratne and Karen C. Crasta

Supplementary information

Supplementary Methods

Supplementary References

Supplementary Figures

Supplementary Tables

Supplementary Methods

Cell lines and culture

Cell lines were maintained at 37°C with 5% CO2 atmosphere and all cell culture media were supplemented with 1% Penicillin/Streptomycin (Invitrogen). For cell synchronisation, cells were treated with 24 h of serum starvation, followed by 24 h of thymidine treatment and 3 h of drug-free media before drug treatment.

Generation of stable cell lines

To establish stable knockdown of TP53 and autophagy-related genes, U2OS cells were infected with viral particles produced from HEK293T cells transfected with targeting plasmids along with lentiviral packaging plasmids. Two days after infection, cells were selected with puromycin (1 μg/ml) for two weeks. The shRNA plasmids used in this study were as follows: shATG5-pLKO.1-puro was cloned using the following targeting sequence (sense): 5’-GACAAGAAGACATTAGTGA-3’ as described previously (1). shp53- pLKO.1-puro (#19119) and pLKO.1-empty vector (control shRNA) (#10878) were purchased from

Addgene. psi-LVRU6MP lentiviral plasmids targeting IL-1β and IL-8 were purchased from GeneCopoeia.

To establish stable expression of GFP-H2B and GFP-LC3, cells were infected with viral particles produced from HEK293T cells transfected with pBabe-puro-H2B-GFP (#26790) or pBabe-puro-GFP-LC3 (#22405)

(Addgene) along with retroviral packaging plasmids.

Flow cytometry

Cells were fixed with ice-cold 70% ethanol overnight. For phospho-histone-H3/propidium iodide (PI) analysis, fixed cells were washed twice in PBS with 0.5% BSA before incubating with 1:500 dilution of phospho-histone H3 antibody conjugated with Alexa Fluor 488 (Cell Signaling) for 1 h in the dark at room temperature. The samples were then washed in 0.5% BSA in PBS and incubated with 250 μg/ml RNase A and 10 μg/ml PI at 37°C for 30 min before analysis. FACS analyses were performed with a BD Accuri C6 flow cytometer (BD Biosciences) using two-parameter scanning; FL1-H (FITC) versus FL2-A (PI) and data were analysed using FlowJo® (FlowJo, LLC). Scratch wound migration assay

Cells were incubated with CM for 2 days. At 90% confluency, the monolayer cell was scratched with a 200

μl pipette tip evenly to generate a wound. To track wound closure rate, live-cell imaging was performed with 20X objective lens as described above. Images were captured every 4 h for 24 h and the distance travelled by cells was measured between the two boundaries of a cellular area using ImageJ. The percentage of wound closure was calculated as follows: [(Area of original wound - Area of actual wound) / Area of original wound] ×100%.

Cell Invasion Assay

U2OS cells expressing chromatin marker H2B-GFP were seeded at 4 x 105 cells per 10 cm dish followed by incubation with CM for 2 days. Cells were then plated on the top surface of transwell filter chambers pre-coated or uncoated with Matrigel (BD Biosciences) and cultured for 24 h. Invasive cells present on the lower surface were fixed in 4% paraformaldehyde and observed under 20X objective lens (5-8 random fields per insert). Percentage of invasive cells was determined using the formula: Invasive cells (%) = (Mean number of cells invading through Matrigel matrix insert membrane / Mean number of cells migrating through uncoated insert membrane) ×100%. Mean number of invasive cells was calculated as average cell number from 8 random fields per condition of 3 independent experiments.

Choroid angiogenesis assay

The choroid tissue from eyes of P3 mice was separated in 10% FBS-PBS solution. The peripheral choroid layer was separated from the retina and cut into approximately 1 mm × 1 mm segments. Each segment was mounted on a 20 μl drop of Matrigel and was incubated with respective CM 1:3 diluted with EGM2 media

(Lonza) over four days. Fresh media was supplemented every other day. Individual explants were imaged under phase contrast on Day 4 using Nikon Eclipse-Ti microscope. The ratio of area of vascular sprouting vs area of the explant was analysed using ImageJ. A minimum of 6 explants was analysed per treatment.

Live-cell imaging

Cells were grown on glass-bottom 6-well plates (MatTek) and imaged on a Nikon inverted fluorescence microscope. The microscope was equipped with EMCCD camera (Photometrics Evolve 512), precision motorised stage and Nikon Perfect Focus. Cells were kept in a controlled atmosphere of 37°C and 5% humidified CO2. Images were captured at multiple sites every 30 min for 48 h - 72 h using a Plan apo 40X objective. Images were processed and analysed by MetaMorph software (Molecular Devices). The duration of mitotic cell death (MCD) was defined as the time of cell rounding to onset of blebbing followed by lysis, whereas mitotic slippage was defined as the time of cell rounding to onset of blebbing followed by cell flattening and reattaching to the glass plate.

Immunoblotting

Cells were lysed in RIPA buffer (Pierce) containing Protease/Phosphatase Inhibitor Cocktail (Thermo

Scientific). Protein concentration was measured using Bradford assay (Bio-Rad). An appropriate volume of Laemmli sample buffer was added to equal amounts of total protein followed by boiling for 5 min.

Samples were subjected to SDS–PAGE electrophoresis before transfer to a nitrocellulose membrane (Bio-

Rad). After overnight incubation at 4°C in primary antibody appropriately diluted in 3% milk (Bio-Rad) in

PBS, membranes were incubated with HRP-conjugated secondary antibodies appropriately diluted in 3% milk in PBS. Signal was detected using enhanced chemiluminescence (ECL) (GE Healthcare Life Sciences) and ChemiDoc IP imaging system (Bio-Rad).

Quantification of cellular migration and metastasis in zebrafish (ZgraftTM)

The Zebrafish Research Facility as well as the Animal Care Committee in Acenzia is certified through

Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) and all protocols used in the laboratories were monitored by the Animal Health and Welfare Branch, OMAFRA. Fishes were housed and maintained using Tecniplast’s ZebTec system with a controlled day-night (14 h light/10 h dark) light cycle in the facility. Embryos were obtained from natural spawnings and developmental stages are reported as hours post-fertilization (hpf) at 28.5°C. CM was prepared from U2OS cells carrying either pLKO.1 control vector or shATG5 plasmid (as described above). U2OS cells carrying chromatin marker H2B-GFP were incubated with CM two days before injection. Xenotransplanted U2OS cells were visualised in the zebrafish embryos with both green fluorescence (H2B-GFP) as well as red fluorescence by staining with

DiI (Vybrant Life Technologies). Cells from each treatment were detached at 80% confluence using

Trypsin-EDTA solution (Sigma). Cells were further incubated for 45 min at 37°C in serum free culture media containing DiI (Vybrant, Life Technologies) at a concentration of 5 μl dye per one million cells.

After incubation, cells were washed twice and re-suspended in serum free media for injection. Zebrafish embryos at 48 hpf were then dechorionated when required and anesthetised with tricaine (Sigma). Using

Nanoject II (Drummond) injector, approximately 100 cells were injected into the yolk of each embryo.

After injection, embryos were incubated for 2 h at 32°C and checked for successful injection. Embryos with fluorescent cells outside the yolk sac were excluded from further experimentation and analysis. Properly injected embryos were further incubated at 32°C in fresh fish tank system water for 48 h and imaged.

Embryos were anesthetised on a microscope slide at 48 h post injection and imaged using Leica M165 LC.

Images were gathered, aligned to a specific orientation and analysed by ImageJ software to determine metastatic tumour foci position relative to injection site (0,0 on graph). Tumour foci observed in all tested larvae, in each treatment group (presented as n on the graph), were presented as scatter plots. All foci in one larva were represented by one colour. For consistent quantitative comparison of the differentially treated cells, Invasion Index measured the ability of cells to invade and Migration Index measured the ability of cells to migrate. These were calculated as follows: Invasion index = Number of metastases formed per 100 cells injected. Migration index = Distance travelled by metastases as percent of the maximum distance cells can migrate within the fish. To rule out the influence of differential proliferation and viability of U2OS cells in the four treatments, the total residual GFP fluorescence intensity of the tumour cells in the larvae was measured using ImageJ as described previously (2). As only the GFP reporter genes drive viable cells to fluoresce, the effects of the treatment on the viability and/or proliferation of the tumour cells were assessed by the fluorescence of the tumour foci in the larvae. Murine xenograft assays

BALB/c athymic nude mice (nu/nu) purchased from InVivos were kept under specific pathogen–free (SPF) conditions. 2.5 x 106 HCT116 colon cancer cells were mixed with 20% BD Matrigel matrix and injected subcutaneously into both dorsal flanks of female nude mice (n = 5 mice in each treatment group). 10 days later, when tumour volume reached around 300 mm3, mice were injected intraperitoneally with vehicle control (10% DMSO, 12.5% Cremophor, 12.5% ethanol, and 65% saline based diluent), Noc (10 mpk),

PTX (10 mpk), chloroquine (CQ, 30 mpk), Noc + CQ (10 mpk:30 mpk) or PTX + CQ (10 mpk:30 mpk) every three days for two weeks. Tumour volume was measured every three days since beginning of treatment by using the formula: (Length × Width2)/2. Mice were sacrificed at the end of the treatment schedule and all tumours were harvested for weight measurement, histochemistry and immunofluorescence.

Tumour tissue was fixed in 10 % buffered neutral formalin solution for histological studies. After fixation, tissues were embedded in paraffin wax and solid sections were cut at 4 μm. The sections were deparaffinised in xylene, hydrated using graded series of alcohol and blocked using 3% BSA in PBS for 1 h at room temperature and then stained according to immunofluorescence protocol described above.

Immunohistochemistry

For paraffin-embedded tissues, the sections were deparaffinised in xylene using graded series of alcohol, rehydrated with decreasing graded series of alcohol and washed in PBS. For cryosectioned tissue, the sections were fixed in 4% paraformaldehyde and washed in PBS. Paraffin-embedded sections were subjected to heated antigen retrieval in citrate buffer (pH 6.0). Tissue endogenous peroxidase activity was blocked using 3% hydrogen peroxide for 15 min at room temperature. Blocking was performed using PBS containing 3% normal goat serum, 1% BSA and 0.1% Triton X-100 for one hour at room temperature. The sections were then incubated with primary antibodies 1:100 diluted overnight at 4°C, followed by incubation with biotinlyated secondary antibodies (Vector laboratories, USA). Next, sections were incubated in Avidin:Biotinylated Enzyme Complex (ABC; Vector Laboratories, USA) for 30 minutes.

Sections were developed with 3,3’-diaminobenzidine (DAB) substrate (Vector Laboratories, USA) to give a brown colour stain and nuclei were counterstained with haematoxylin. Slides were mounted with Fluka Eukitt® quick-hardening mounting medium (Sigma-Aldrich, USA). Negative controls were carried out in the absence of the primary antibody. Images were taken using Carl Zeiss slide scanner Axio Scan.Z1 using a Plan-Apochromat 40x/0.95 NA objective and Hitachi HV F202, and ZEN 2 slidescan software. The immunohistochemical stained image was scored blind by two of the authors (X.F.W. and H.C.C.). Intensity of imaged sections was scored by negative-0, weak-1, moderate-2 or strong-3 positive staining intensity.

Percentage of the positively stained tumour cells at several representative regions were recorded as numerical score in every 10% (Supplementary Table 5). H scores were then calculated by summing the product of staining intensity (0-3) and numerical value from the percentages of stained cells to each intensity

(0-100). H scores ranged from 0 to 300. The observer differences were less than 5%, and the mean of the two values were obtained.

SILAC Labelling

RPE-1 cells were cultured in ready-to-use light and heavy SILAC DMEM/F12 media, containing light or

12 12 heavy arginine and lysine (“R0K0 DMEM/F12” with C6 -L-arginine (Sigma Aldrich) and C6 -L-lysine

13 13 (Sigma Aldrich) or “R10K8 DMEM/F12” with C6 -L-arginine (Cambridge Isotope) and C6 -L-lysine

(Cambridge Isotope), respectively), and supplemented with dialyzed FBS. SILAC labelling was performed as previously described (3). For forward SILAC experiments, light-labelled cells were treated with DMSO and heavy-labelled cells were treated with Noc. For reverse SILAC experiment, heavy-labelled cells were treated with DMSO and light-labelled cells were treated with Noc. Cells were harvested by trypsinisation and lysed using RIPA buffer (Thermo Scientific) with Protease/Phosphatase Inhibitor Cocktail (Thermo

Scientific). Protein concentration was measured using Bradford assay (Bio-Rad). Equal amounts of protein from R0K0 and R10K8 were mixed and boiled with loading buffer at 95°C for 5 min. Eluted protein complexes were separated by one-dimensional 4–12% NuPage Novex Bis–Tris Gel (Invitrogen), stained with Colloidal Blue Staining Kit (Invitrogen) and digested with trypsin using in-gel digestion as described previously (3). Mass spectrometry and data analysis

Tryptic peptides were analysed using Thermo Scientific Easy-nLC with 15 cm column (Acclaim PepMapTM

RSLC, C18, 100 Å, 2 μm, 75 mm × 15 cm) coupled with Orbitrap XL (Thermo Scientific). Peptides were loaded with buffer A (0.1% formic acid, 2% acetonitrile) and eluted with a 120 min gradient at a flow rate of 250 nl/min from 8-40% buffer B (0.1% formic acid, 80% acetonitrile). Mass spectra were acquired in positive ion mode. Survey full scan MS spectra (m/z 310–1400) were acquired with a resolution of r = 60,000, an AGC target of 1e6, and a maximum injection time of 700 ms. The ten most intense peptide ions in each survey scan with an ion intensity of >2000 counts and a charge state ≥2 were isolated sequentially to a target value of 1e4 and fragmented in the linear ion trap by collisionally-induced dissociation using normalized collision energy of 35%. A dynamic exclusion was applied using a maximum exclusion list of 500 with one repeat count and 30 s repeat, exclusion duration of 60 s. SILAC peptide and protein quantification was performed with MaxQuant version 1.5.0.30 using default settings. Database searches of MS data were performed using Uniprot human FASTA (2015 Jan release, 89706 proteins) with tryptic specificity allowing maximum two missed cleavages, two labeled amino acids, and an initial mass tolerance of 6 ppm for precursor ions and 0.5 Da for fragment ions. Cysteine carbamidomethylation was searched as a fixed modification, and N-acetylation and oxidized methionine were searched as variable modifications. Labeled arginine and lysine were specified as fixed or variable modifications, depending on the prior knowledge about the parent ion. Maximum false discovery rates were set to 0.01 for both protein and peptide. Proteins were considered identified when supported by at least one unique peptide with a minimum length of seven amino acids.

Microarray and data analysis

Synchronised U2OS cells were treated with DMSO or Noc for 48 h, followed by total RNA extraction using

RNeasy plus Mini Kit (QIAGEN) according to manufacturer’s instructions. RNA yield quantity and quality were assessed using NanoDrop Spectrophotometer (NanoDrop Products, Thermo Fisher Scientific, Inc.,

Wilmington, DE, USA) and Agilent Bioanalyzer with Agilent 6000 Nano RNA kit (Agilent Technology,

Santa Clara, CA, USA). Samples were considered acceptable for testing when 28S and 18S rRNA bands resolved into two discrete bands that had no significant smearing below each band with RIN value more than 8.0. Double stranded cDNA was generated from the RNA sample using the GeneChip® WT PLUS

Reagent Kit according to the manufacturer protocol. Fragmentation and end-terminus labeling of cDNAs were done using the same kit according to the manufacturer protocol. The quality and concentration of cDNAs were measured using NanoDrop Spectrophotometer and only 5.5 μg of cDNA will be used for microarray analysis. The samples were then hybridized to GeneChip® Human Transcriptome Array 2.0 at

45°C for 16 h overnight, following manufacturer procedures. GeneChips post-processed were done on the

AFX Fluidics 450 Station, according to all AFX protocols and procedures defined for the Human

Transcriptome Array 2.0 (FS450_0001), as outlined in the kit manual. GeneChips were scanned on the

GC3000 G7 Scanner. Data were extracted and processed using Partek® Genomics Suite™ (Partek

Incorporated, St. Louis, MO,USA). For microarray data analysis, raw microarray .cel files were imported into Partek Genomic Suite (v7.0) (Partek Inc., St. Louis, MO) with the core meta-probe set. The configuration consisted of a pre-background adjustment for GC content and probe sequence, Robust Multi- array Analysis (RMA) for background correction, quantile normalization and probe set summarization using median computation from the raw data of each file. All signals were log2 transformed. Library files were those specified by Affymetrix (Human Gene 2.0 AGCC library file) and the annotation file version was HTA-2_0.na36.hg19.transcript.csv. For determination of differential expression, probe set information is summarized into gene level information. The ANOVA was performed for all samples with contrasts set to type of drug treatment. Genes differentially expressed by >2.5 and <-2.5 folds between two biological replicates of Noc versus DMSO with an unadjusted p-value of less than 0.05 were selected and examined.

Hierarchical clustering was created based on all annotated genes from the selected differential gene expression gene list. The microarray data have been deposited in the GEO repository under accession number GSE114515. Statistical methods

All statistical analyses were performed using GraphPad Prism 6. Significance levels for comparisons between two groups were determined with two-tailed Student t-test. Significance levels for comparisons of more than two groups were determined with two-way analysis of variance (ANOVA).

Supplementary References

1. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, et al. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 2006;124(6):1283-98. 2. Teng Y, Xie X, Walker S, White DT, Mumm JS, Cowell JK. Evaluating human cancer cell metastasis in zebrafish. BMC Cancer 2013;13:453. 3. Swa HL, Shaik AA, Lim LH, Gunaratne J. Mass spectrometry based quantitative proteomics and integrative network analysis accentuates modulating roles of annexin-1 in mammary tumorigenesis. Proteomics 2015;15(2-3):408-18.

Supplementary Figures

Supplementary Figure S1. Post-slippage cells arrest at G1 as tetraploid multinucleated cells.

Supplementary Figure S2. Correlation of multinucleation and senescence post-slippage in response to various anti-mitotic drugs.

Supplementary Figure S3. In vivo association of senescence and anti-mitotic drug treatment.

Supplementary Figure S4. Post-slippage cells increase expression of factors associated with senescence and SASP.

Supplementary Figure S5. SASP factors from post-slippage cells do not affect proliferation of neighbouring cells.

Supplementary Figure S6. Autophagy mediates senescence.

Supplementary Figure S7. Autophagy does not modulate cell fate during prolonged mitotic arrest.

Supplementary Figure S8. Autophagy-dependent IL-1β and IL-8 expression mediate cell invasiveness in a paracrine manner.

Supplementary Figure S9. Silencing of p53 increases post-slippage cell death.

Supplementary Figure S10. p53 status determines synergistic effect of microtubule poisons and autophagy inhibition.

Supplementary Figure S1

Addition ABSeeding Serum Release from of Noc of cells starvation Thymidine thymidine t = 0 h

1 day1 day 1 day 3 hours 72 hours Synchronisation Live-cell imaging Noc (h)

t = 16 h U2OS cyclingsyn 0 1216202430364872 U2OS RPE-1 100 93.1 92.4 Cyclin B1 80

60 p-HH3 (Ser10)

40 (t = 0 h) BubR1 Slippage marker

Addition of Noc 20 Mitotic index (%) Bub1 0 U2OS RPE-1 t = 72 h Mcl-1 100 87.8 (t = 16 h) marker cleaved PARP

80 Apoptosis Mitotic arrest 70.9

60 GAPDH

40

20 (t = 72 h) Mitotic slippage (%)

Mitotic slippage 0 U2OS RPE-1

C U2OS Cyc Noc 20 h

Mitotic cells Mitotic cells

G1-S phase G2 phase G1-S phase G2 phase

0 200400600 800 1000 0 200400600 800 1000

Noc 24 h Noc 48 h

Mitotic cells Mitotic cells phospho-Histone H3 (Ser10)-FITC Tetraploid Tetraploid G1-S phase cells G1-S phase cells

0 200400600 800 1000 0 200400600 800 1000

DNA content/PI staining

U2OS HCT116 U2OS DFDICH2B-GFP Merge E Merge 12h DIC DNA Merge BrdU DNA

100 DMSO

**** DMSO **** **** 80 18h 60 Noc 72h Noc 48h 40 80 100 **** Noc

20 80 60

Multinucleated cells (%) 60 0 30h 40 0 1624364872 40 Noc (h) 20 20 BrdU-positive cells (%) Multinucleated cells (%) 0 48h 72h 0 Noc DMSO Noc (72 h) Supplementary Figure S1. Post-slippage cells arrest at G1 as tetraploid multinucleated cells. A, U2OS and RPE-1 cells were treated with Noc and subsequently visualised by live-cell imaging to track individual cell fates. Representative images of cells arrested in mitosis at t = 16 h and interphase cells that have undergone mitotic slippage at t = 72 h are shown. The top plot shows mitotic index, which depicts the percentage of mitotic cells among total cell population at 16 h post-Noc treatment. The bottom plot shows the percentage of mitotic slippage, which depicts cells that displayed blebbing and re-flattening following prolonged mitotic arrest. 5 random fields taken per condition with 3 independent experiments. B, U2OS cells were treated with Noc for the indicated duration, subjected to immunoblotting and probed for slippage and apoptosis markers. C, U2OS cells were treated with Noc for the indicated duration. Mitotic slippage was assessed by flow cytometry after co-staining with propidium iodide (PI) and FITC-conjugated phosphorylated Histone H3. Numbers in each quarter indicate the percentage of different cell populations. D, Live-cell imaging of U2OS H2B-GFP cells shows post-slippage multinucleation in response to Noc treatment. Plot shows percentage of multinucleated cells. n = 200 cells and mean ± s.d. of 3 independent experiments. E, Representative images depict multinucleation of HCT116 cells treated with indicated Noc for the indicated duration. Plot shows percentage of multinucleated cells. n = 200 cells per condition and data are mean ± s.d. of 2 independent experiments. Scale bar 20 μm. F, Proliferation of U2OS cells were measured by BrdU incorporation with or without Noc treatment for 72 h. Plot shows percentage of BrdU-positive cells. n = 100 cells and data are mean ± s.d. of 3 independent experiments. ****P<0.001. Scale bar 20 μm. Supplementary Figure S2

U2OS (t = 72 h) HCT116 A B Noc DMSO 48h 72h DMSO PTX

50 *** * 100 *** ** *** 40 80

30 ** 60 ** PTX ZM Mon 20 40 48h 72h -gal positive cells (%) -gal positive cells (%) ȕ

ȕ 10 20 SA- SA- 0 0 DMSO 48h 72h 48h 72h ZM PTX Mon Noc PTX DMSO

C PTX ZM K 24 h 28 h K 24 h 28 h U2OS H2B-GFP DIC Merge Mon K 24 h 28 h ns *** 100 ***

75

50 U2OS

H2B-GFP DIC 25 Multinucleated cells (%) 0

Merge PTX ZM Mon DMSO

Supplementary Figure S2. Correlation of multinucleation and senescence post-slippage in response to various anti-mitotic drugs. A, U2OS cells were treated with DMSO control, Paclitaxel (PTX), ZM447439 (ZM) or Monastrol (Mon) for 72 h and assessed for senescence by 6$ȕJDOVWDLQLQJ3ORWVKRZVWKHSHUFHQWDJHRI6$ȕJDOSRVLWLYHFHOOVFHOOVSHUFRQGLWLRQDQGGDWDDUHPHDQ“VGRILQGHSHQGHQW H[SHULPHQWV6FDOHEDU—P B, +&7cells were treated with DMSO control, Nocodazole (Noc) or PTX for 72 h and assessed for VHQHVFHQFHE\6$ȕJDOVWDLQLQJ3ORWVKRZVWKHSHUFHQWDJHRI6$ȕJDOSRVLWLYHFHOOVFHOOVSHUFRQGLWLRQDQGGDWDDUHPHDQ“VGRI LQGHSHQGHQWH[SHULPHQWV6FDOHEDU—PC, 826+%*)3FHOOVZHUHWUHDWHGZLWKLQGLFDWHGDQWLPLWRWLFGUXJVDQGFHOOIDWHVZHUH WUDFNHGLQGLYLGXDOO\E\WLPHODSVHPLFURVFRS\3ORWVKRZVWKHSHUFHQWDJHRISRVWVOLSSDJHPXOWLQXFOHDWHGFHOOVQ FHOOVSHUFRQGLWLRQDQG GDWDDUHPHDQ“VGRILQGHSHQGHQWH[SHULPHQWV6FDOHEDU—P 3  3  3 DQGQVQRWVLJQLILFDQWE\6WXGHQW¶V WWHVW Supplementary Figure S3

A HCT116 B HCT116 Xenograft mouse model Xenograft mouse model 10 Veh Ctrl Noc PTX 9 2.0 8 1.8 7 1.6 1.4 H&E 6 1.2 5 1.0

gal (%) area 4 0.8 ȕ 3 0.6 ȕJDO SA- 2 p21 mRNA level 0.4 0.2 Relative fold change of SA- 1 0.0 0 Veh Noc PTX Veh Noc PTX Ctrl Ctrl

CDHCT116 H&E staining p21 / DAPI Xenograft mouse model

p21 Haematoxylin

Veh Ctrl Pre-treatment

Noc BC36.2 BC90.1 BC113.1 PTX Invasive ductal carcinoma of the human breast tissues

** Post-PTX treatment 250 **** BC120.1

200

150 16

score 14 H 12 100

p21 10 8 50 6 4 2 0 Veh Noc PTX p21 positive area (%) 0 Ctrl BC113.1 BC90.1 BC36.2 BC120.1

Supplementary Figure S3. In vivo association of senescence and anti-mitotic drug treatment. A, Tumour sections from human HCT116 colon cancer xenografts in mice after treatment with vehicle control (Veh Ctrl), Noc or PTX were stained for haematoxylin and eosin (+ ( DQG6$ȕJDO ,QVHUWVLQWKHUHSUHVHQWDWLYHLPDJHVVKRZHGPXOWLQXFOHDWHGFHOOV3ORWVKRZVWKHSHUFHQWDJHRI6$ȕJDOSRVLWLYHDUHDScale bar 100 μm B, Cryosections of tumours for each treatment were pooled and subjected to qRT-PCR for p21P51$OHYHOVZHUHQRUPDOLVHGWR*$3'+DQG9HK&WUO C, Immunohistochemical analysis of p21 expression in human HCT116 colon cancer xenografts in mice after treatment with vehicle control (Veh Ctrl), 1RFRU37;5HSUHVHQWDWLYHLPDJHVZHUHVKRZQZLWKPDJQLILHGLQVHUWVRQWKHULJKW6FDOHEDU—P'RWJUDSKVKRZVH score of p21 expression ZLWKPHDQPDUNHGE\DK\SKHQDWLRQn WXPRXUVSHUWUHDWPHQWJURXSD, Representative images from tumour sections from invasive ductal carcinoma of the human breast pre or post PTX treatment stained for + (RUS3ORWVKRZVTXDQWLILFDWLRQRIWKHZKROHWXPRXUVHFWLRQV6FDOHEDU 100—P 3  3 E\6WXGHQW¶VWWHVW Supplementary Figure S4 ACB

IL-1B Histone H3.3 Fwd DMSO Nocodazole IL-8 Rev Nocodazole DMSO

MAP1A Ratio H/L 2

light (L) heavy (H) GABARAP

Fwd: Log MAP1LC3B MAP1B

Lyse & Mix

-6 -4 -2 0Spearman 2 Correlation 4 = -0.63 -4-20246

Rev: Log2 Ratio H/L 1D SDS PAGE Protein hits from SILAC Mass-spectrometry Log fold change in Log fold change in In-gel trypsin digestion Forward set Reverse set Senescence-related proteins IL-1B 2.4 -3.2 IL-8 1.3 -1.7 Histone H3.3 2.3 -2.2 Autophagy substrates nLC-Orbitrap MS MAP1A -1.1 0.9 MAP1B -1.2 0.8 MaxQuant analysis MAP1LC3B -1.5 1.2 GABARAP -1.2 0.5

Supplementary Figure S4. Post-slippage cells increase expression of factors associated with senescence and SASP. A, Total mRNA extracted from U2OS cells treated with Noc or DMSO for 48 h was subjected to microarray. Top hits are shown for genes that are at least 2.5-fold upregulated (red) or downregulated (green) after treatment. B, Schematic workflow of SILAC mass spectrometry-based analysis of cellular proteins where control-treated (DMSO) or Noc-treated RPE-1 cells are labelled with ‘light’ and ‘heavy’ amino acids, respectively and vice-versa. C, Scatter plot (correlation plot) shows up-regulated (red) and down-regulated (blue) proteins with at least 1.5-fold change upon Noc treatment. Table lists hits studied here. Supplementary Figure S5

A t = 96 h

BrdU DNA Merge BrdU DNA Merge 80 66 67

60 49.0 40.5 DMSO CM DMSO CM 40 U2OS RPE-1 20

BrdU-positive cells (%) 0 Noc CM Noc CM CM: DMSO Noc DMSO Noc U2OS RPE-1

B 150 RPE-1 + Noc CM RPE-1 + DMSO CM )

4 U2OS + Noc CM U2OS + DMSO CM 100

50 Cell number (10

D0 D2 D4 D6

Supplementary Figure S5. SASP factors from post-slippage cells do not affect proliferation of neighbouring cells. A, Representative images of BrdU incorporation in U2OS and RPE-1 cells 4 days after culturing with indicated CM. Plot shows percentage of BrdU-positive cells. 100 cells per condition and number on top of each bar represents mean value ± s.d. of 3 independent experiments. Scale bar 100 μm. B, U2OS and RPE-1 cells were pre-incubated with indicated CM and seeded into 6-well plates at 5000 cells/well. Cell number was counted every two days and plotted as a growth curve. Data are mean ± s.d. of 3 independent experiments. Supplementary Figure S6

A U2OS RPE-1 HCT116 t = 72 h 100 Noc Noc + Baf A1 *** 80 Noc ***

60 ***

40

Noc + 20 -gal positive cells (%)

Baf A1 ȕ 0

SA- U2OS RPE-1 HCT116

B Collect Collect Seeding Start serum Start Release from Start Noc samples samples of cells starvation thymidine thymidine & Baf A1 Day 3 Day 6 1 day1 day 1 day 3 hours 3 days 3 days Synchronisation+/-Baf A1 +/-Noc Drug-free media

Removal of drug treatment and release to fresh media for additional days

CDRPE-1 RPE-1 Noc (Day) - 3 6 3 6 1.0 DMSO Baf A1 - - - + + Baf A1

Lamin B1 0.8

LC3-I 0.6 LC3-II

LC3-II/GAPDH 0.02 0.3 0.12 1.77 1.89 0.4 ratio B1 mRNA level B1 mRNA p62 0.2 Relative fold change of lamin GAPDH 0.0 Noc (Day) - 3 6

Supplementary Figure S6. Autophagy mediates senescence. A, Representative images of U2OS, RPE-1 and HCT116 cells treated ZLWK1RFLQSUHVHQFHRUDEVHQFHRI%DI$IRUKDQGVWDLQHGIRU6$ȕJDO3ORWVKRZVSHUFHQWDJHRI6$ȕJDOSRVLWLYHFHOOVQ  cells per condition and data are mean ± s.d. of 3 independent experiments. 6FDOHEDUμm. B, Experimental scheme of RPE-1 cells treated with Noc for 3 and 6 days in the presence or absence of Baf A1 pre-treatment. C, Cell lysates collected as per (B) were subjected to IB and probed for autophagy- and senescence-associated markers. D, Cell lysates collected as per (B) were subjected to qRT-PCR analysis for relative lamin B1 expression levels. mRNA levels were normalised to GAPDH and DMSO control. Data are mean ± s.d. of 3 independent experiments. 3  Supplementary Figure S7 AB

80 2000 Mitotic slippage ns ns Mitotic cell death (MCD) 60 1500

40 1000

Cell Count 20 500 to slippage (min)

0 Duration from mitosis shCtrl shATG5 shCtrl shATG5 0 shCtrl shATG5 shCtrl shATG5 Noc PTX Noc PTX

CDNoc BafA1 Noc+Baf A1 Time (h) 0 16 24 36 48 60 72 16 16 24 36 48 60 72

BubR1 siCtrl siATG5 Noc (h) 16 24 36 48 16 24 36 48 Cyclin B1 Cyclin B1

p-HH3(Ser10) ATG5

GAPDH GAPDH

Supplementary Figure S7. Autophagy does not modulate cell fate during prolonged mitotic arrest. A, U2OS shCtrl or shATG5 were captured by live-cell imaging during 48 h of treatment with Noc or PTX to examine pre-slippage cell fate [mitotic slippage versus mitotic cell death (MCD)]. Plot shows quantification of cells with indicated fates. Red: Cells that undergo mitotic slippage. Black: Cells that die in mitosis. B, Duration from mitosis to slippage in U2OS cells treated with Noc or PTX. Dots represent individual cells with mean of data indicated by a black line. C, U2OS cells were treated with Noc in absence or presence of Baf A1 for the indicated duration and subjected to IB analysis. D, U2OS cells were transfected with siCtrl or siATG5 followed by treatment with Noc for the indicated duration and subjected to IB analysis. ns not significant by Student’s t-test. Supplementary Figure S8

A U2OS B C t = 72 h

t = 72 h 2.0 U2OS shCtrl shATG5 shCtrl *** Noc (72 h) --++ VK,/ȕ shATG5 U2OS shCtrl VK,/ȕ shIL-8 + shIL-8 1.5 ,/ȕ ,/ȕ ns *** **** **** IL-8 1.0 IL-8

mRNA level mRNA ATG5 0.5 GAPDH

Relative fold change of GAPDH 0.0 IL-1Į IL-1ȕ IL-6 IL-8 BMP2

D U2OS H2B-GFP E U2OS DMSO CM Noc CM 8 150 DMSO CM DMSO CM Noc CM Noc CM **** shCtrl ** 6 ns ns **** 100 ns **** /ȕ 4

shI *

50 2 ns shIL-8 ns ns ns Relative fold change of mRNA level

Mean number of invaded cells 0 0

/ȕ ȕ ȕ ȕ ȕ ȕ ȕ +IL-8 shI shIL-8 shCtrl shIL-8 shCtrl shIL-8 shCtrl shIL-1 shIL-1 shIL-1 shIL-1 shIL-1 shIL-1 + shIL-8 + shIL-8 + shIL-8 Fibronectin MMP-9

F U2OS H2B-GFP DMSO CM Noc CM ** 150 **** * * 100 ns shCtrl shATG5

50 VK$7*,/ȕ

invaded cells shATG5 + IL-8 Mean number of shCtrl VK$7*,/ȕ,/ 0

Noc CM Noc CMNoc CMNoc CMNoc CM DMSO CM DMSO CM Noc CM + DMSO CM Noc CM Noc CM + I/ȕ Noc CM + IL-8 I/ȕ,/ shATG5

6XSSOHPHQWDU\)LJXUH6$XWRSKDJ\GHSHQGHQW,/ȕDQG,/H[SUHVVLRQPHGLDWHFHOOLQYDVLYHQHVVLQDSDUDFULQHPDQQHU A, U2OS cells stably expressing shCtrl or shATG5 were treated with or without Noc for 72 h, followed by qRT-PCR of the indicated SASP components. mRNA levels were normalised to GAPDH and DMSO control. B, Cell pellets treated as in (A) were lysed and subjected to IB analysis. C, U2OS FHOOVVWDEO\H[SUHVVLQJS6L/95803 VK&WUO VK,/ȕVK,/RUVK,/ȕDQGVK,/ZHUHWUHDWHGZLWK1RFIRUKDQGVXEMHFWHGWR,%ZLWK indicated antibodies. D, U2OS H2B-GFP cells were incubated with DMSO CM or Noc CM from indicated cell lines for 2 days and assessed for LQYDVLRQE\WUDQVZHOODVVD\3ORWVKRZVTXDQWLILFDWLRQGDWDRIPHDQQXPEHURILQYDGHGFHOOVUDQGRPILHOGVZHUHWDNHQSHUFRQGLWLRQ'DWD indicates mean ± s.d. of 3 independent experiments and analysed by two-way analysis of variance (ANOVA). E, U2OS cells were treated as in (D DQGVXEMHFWHGWRT573&5DQDO\VLVRIPLJUDWLRQDVVRFLDWHGPDUNHUVP51$OHYHOVZHUHQRUPDOLVHGWR*$3'+DQG'062FRQWURO'DWD indicates mean ± s.d. of 3 independent experiments and analysed by two-way ANOVA. F, U2OS H2B-GFP cells were incubated with DMSO &0RU1RF&0IURP826VK&WUORU&0IURPVK$7*VXSSOHPHQWHGZLWKUHFRPELQDQW,/ȕ,/RUERWK,QYDVLYHDELOLW\ZDVDVVHVVHGE\ WUDQVZHOODVVD\3ORWVKRZVTXDQWLILFDWLRQGDWDRIPHDQLQYDVLYHFHOOVUDQGRPILHOGVZHUHWDNHQSHUFRQGLWLRQ'DWDLQGLFDWHVPHDQ“VGRI 3 independent experiments analysed by Student’s t-test. *P<0.05, **P<0.005, ***P<0.001, ****P<0.0001 and ns, not significant. Supplementary Figure S9

A 826 W K B shCtrl shp53

 DMSO Noc PTX * 826 W K DMSO Noc PTX DMSO Noc PTX 100 ** cleaved PARP 80

Ȗ+$; 60

40

% Cell Viability p53

 *$3'+ 0 shCtrl shp53

Supplementary Figure S9. Silencing of p53 increases post-slippage cell death. A, U2OS shCtrl (pLKO.1) or shp53 were treated with indicated drugs for 72 h. Cell viability was assessed using CellTiter 96® One Solution Proliferation. Data are mean ± s.d. of 3 independent experiments. B, U2OS shCtrl or shp53 cells were treated with indicated drugs for 72 h. Lysates from post-slippage cells were prepared for IB and probed against apoptosis- and DNA damage-related markers. *P<0.05 and **P<0.005 by Student’s t-test. Supplementary Figure S10

A t = 72 h DMSO Baf A1 1.5 Noc PTX Noc + Baf A1 PTX + Baf A1 * 1.0 ns ns ns ns ns ns ns

0.5 Relative cell viability

0.0 MCF7 MDA-MB-231 PaCa2 PanC1 (p53 WT) (p53 R280K) (p53 R273H) (p53 R248H) BC LC3 E-Cad DNA Merge p62 ȕ-Catenin DNA Merge Veh Ctrl Veh Ctrl Veh Noc Noc Noc Noc + CQ + CQ PTX PTX PTX + CQ PTX + CQ

Supplementary Figure S10. p53 status determines synergistic effect of microtubule poisons and autophagy inhibition. A, A panel of cell lines with wild type (WT) or mutant p53 were treated with indicated drugs and CellTiter 96® One Solution Proliferation assay was measured at 72 h post-treatment to assay viability. Data are mean ± s.d. of 3 independent experiments. B-C, Immunofluorescence analysis of tumour sections from single and combination drug-treated xenograft mice probed against autophagy-related antibodies LC3 and p62. Scale bar 20 μm. *P<0.05 and ns, not significant by Student’s t-test. Supplementary Tables

Supplementary Table S1. List of differentially regulated proteins after 48 h Noc treatment detected by

SILAC mass spectrometry.

Supplementary Table S2. List of antibodies.

Supplementary Table S3. List of chemicals.

Supplementary Table S4. List of primers.

Supplementary Table S5. Quantitative H scoring method Supplementary Table S1. List of differentially regulated proteins after 48 h Noc treatment detected by SILAC mass spectrometry. Analysis of SILAC mass spectrometry-based forward (Fwd) and reverse (Rev) experiments of cellular proteins in 48 h Noc-treated and DMSO-treated RPE-1 cells labelled with ‘light’ (L) and ‘heavy’ (H) amino acids, respectively and vice-versa.

Ratio H L Ratio H L Ratio H L Ratio H L Ratio H L Ratio H L normalized normalized normalized normalized normalized normalized Gene names Fwd Rev Color Gene names Fwd Rev Color Gene names Fwd Rev Color SRSF10 -0.318074 6.342964 black KIF1C -0.508568 0.148739 black WNK1 -0.164302 -0.076569 black KIAA0101 -4.480229 5.486779 blue NCKAP1 -0.065763 0.148609 black TOMM70A 0.048515 -0.076600 black RRM2 -3.871506 4.344970 blue TPRKB 0.036327 0.148218 black SF3A2 0.264957 -0.076737 black CKS1B -1.741734 3.115866 blue NUP133 -0.175387 0.147958 black PSMD5 -0.073606 -0.076782 black ANLN -3.156627 3.073992 blue ADSS -0.186837 0.147437 black NARS -0.268487 -0.076782 black TYMS -2.611667 2.734569 blue DFNA5 0.155361 0.147176 black IAH1 0.161178 -0.076813 black DIAPH3 -2.338396 2.693007 blue CTBP2 0.024887 0.147176 black SNX6 0.220082 -0.076843 black CYR61 -1.184293 2.278788 blue RPL37A -0.001010 0.147176 black PRKAA1 0.076559 -0.077970 black DNMT1 -2.221085 2.142348 blue PNPLA6 -0.493865 0.146916 black TM4SF1 0.482642 -0.078168 black CTGF -2.335991 2.035202 blue ERLIN2 -0.298531 0.146786 black GPD2 0.146003 -0.078350 black ID1 -0.895744 1.945420 blue TWF1 -0.119651 0.146655 black CAPN1 0.130008 -0.078411 black TPM1 -0.365518 1.897047 black PMVK 0.053528 0.146003 black PARK7 0.001586 -0.079966 black PDCD11 -1.622865 1.828428 blue DDX21 -0.104449 0.145482 black DSCR3 -0.337649 -0.080042 black TPM1 -1.347986 1.569880 blue CPNE1 -0.173173 0.145221 black GRWD1 -0.147058 -0.080362 black PRIM2 -1.953607 1.535107 blue COX17 -0.336901 0.144960 black APOL2 0.234624 -0.080423 black FADS2 -1.910502 1.489594 blue LZIC -0.150337 0.144569 black TACO1 -0.190668 -0.080652 black GSKIP -0.403065 1.469730 black NUP153 -0.728048 0.144438 black ZYX -0.460112 -0.081202 black TTI1 -1.075003 1.467593 blue NDUFAF3 -0.307466 0.144177 black MSRA 0.147046 -0.081217 black F3 -1.030266 1.427016 blue CSNK1A1 -0.627962 0.144177 black HSPB1 0.040261 -0.081385 black BPTF -0.301213 1.426855 black STX12 -0.199322 0.143916 black PSMG3 -0.245215 -0.081705 black C19orf43 -1.411848 1.422018 blue CCT5 -0.027337 0.143785 black HPCAL1 -0.031887 -0.082118 black MICAL2 -0.678349 1.400265 blue HUWE1 -0.207311 0.143655 black RAB7A -0.084732 -0.082148 black TNS1 -1.786273 1.366700 blue CDIPT -0.076615 0.143524 black TNPO1 0.077927 -0.082179 black TGFB1I1 -1.562304 1.354734 blue CAP1 0.049352 0.143393 black SRRM2 -0.699724 -0.082362 black UTP20 1.596840 1.321870 black SGPL1 -0.209762 0.143263 black SSBP1 0.146395 -0.082530 black UCK2 -1.480035 1.321120 blue ANXA11 -0.114519 0.143132 black PARP4 -0.256942 -0.083279 black KANK2 -1.497568 1.296134 blue SUMO3 -0.256838 0.143132 black CHMP4B 0.092478 -0.083401 black CDK2 -1.140253 1.267835 blue SUMO2 -0.006159 0.143002 black USO1 0.306379 -0.083493 black FANCI -1.422134 1.266277 blue 5-Mar -0.313119 0.143002 black NUP50 -0.097579 -0.083753 black HAT1 -1.413346 1.262493 blue SSRP1 -0.259893 0.142871 black ARL3 -0.110838 -0.083829 black ADI1 -0.965897 1.257735 blue MLLT4 -0.386431 0.142609 black PPP1R2 0.124990 -0.083967 black ZWILCH -1.272541 1.246591 blue HYPK -0.168982 0.142348 black LYPLA1 0.064469 -0.084058 black CLUH -1.226307 1.239642 blue PDLIM4 -0.230719 0.141564 black TOR1A 0.130272 -0.084410 black ASNS -0.944638 1.211448 blue QDPR -0.097533 0.141040 black SYMPK -0.156595 -0.084594 black C7orf50 -1.080820 1.199248 blue AKR7A2 -0.246960 0.141040 black TXNL1 -0.281176 -0.084609 black NOC3L -1.281737 1.188717 blue RPL31 -0.095820 0.140910 black HEXIM1 -0.118727 -0.084640 black TK1 -1.812992 1.187641 blue RBM4 -0.536210 0.140910 black C15orf40 -0.154458 -0.084854 black EPHB2 -0.565268 1.178874 black TRIP11 -0.421824 0.140779 black ABCC1 0.025171 -0.085129 black CCDC50 -1.069422 1.171591 blue ILKAP -0.163559 0.140386 black NDUFV1 -0.020823 -0.085298 black KIF11 -1.090834 1.164851 blue CALM1 0.051581 0.140124 black TPP2 0.056306 -0.085650 black MAP1LC3B -1.482049 1.159823 blue EXOC5 -0.309538 0.140124 black PRKAG1 -0.165531 -0.085788 black AKAP2 -1.056851 1.154972 blue GYG1 0.265917 0.139993 black YKT6 -0.039577 -0.085910 black CDK1 -1.514903 1.151989 blue DNAJC2 0.056584 0.139731 black ARMCX1 -0.416134 -0.086017 black CKS2 -0.568473 1.148934 black HCFC1 -0.001126 0.139469 black PSMC3 0.024178 -0.086247 black NOC2L -1.196674 1.120286 blue BCL2L1 -0.025074 0.139077 black TOR1AIP1 0.054362 -0.086569 black AFAP1 -0.925741 1.113500 blue SRC 0.018492 0.138945 black RPL5 0.058247 -0.086691 black ABI2 1.194024 1.113100 black GLRX3 -0.369781 0.138945 black AP1S1 -0.129308 -0.086936 black TELO2 -1.024326 1.105946 blue DIAPH1 -0.182360 0.138290 black SSR3 0.086512 -0.086982 black CHURC1 -1.747294 1.104068 blue MRPL46 0.043904 0.137635 black 10-Sep -0.003524 -0.087105 black FAM127B -1.296191 1.091260 blue TTC37 -0.019229 0.137372 black XPO7 0.005328 -0.087243 black C4orf27 -0.574691 1.083384 black KIF13B 0.070252 0.137241 black DARS 0.341303 -0.087335 black CAPRIN1 -1.134667 1.077516 blue FLII -0.154747 0.137241 black NEB 0.357946 -0.087488 black CD99 -1.081858 1.073683 blue PRPF31 -0.137854 0.137110 black TXNDC5 0.035765 -0.087810 black LARP4 -1.678672 1.052694 blue AKAP9 -0.480982 0.137110 black PDIA4 0.063227 -0.088423 black WDHD1 -1.021336 1.050328 blue PEPD -0.147442 0.136454 black RDH14 -0.153928 -0.088485 black NOP9 -0.150065 1.048655 black TBCB -0.170654 0.136454 black MOGS 0.063779 -0.088638 black NOL6 -1.515563 1.041243 blue LSM6 0.133169 0.136323 black PRKAR2A 0.039840 -0.088761 black TIMM8B -0.959711 1.039349 blue ZC3H15 -0.071133 0.136060 black MRPL48 -0.013509 -0.088868 black TUBB2A -0.731637 1.015997 blue PSMD11 -0.048890 0.135666 black PPP2R4 0.130008 -0.089006 black FHL2 -0.857678 1.005472 blue CCDC53 -0.068483 0.135535 black TSC22D1 -0.170816 -0.089359 black TUBA1C -0.845158 1.004681 blue MTOR -0.312975 0.135535 black FARSA 0.009060 -0.089498 black UHRF1 -1.129324 1.001874 blue RAB35 0.107420 0.135404 black KDELR2 0.243913 -0.089528 black ASNSD1 -0.086707 1.001298 black SYNJ2 -0.292157 0.134878 black LSM3 -0.215010 -0.089574 black PHLDA3 -0.791158 0.997401 blue RPS27 -0.351866 0.134878 black SH3GL1 -0.330955 -0.089620 black LMOD1 0.105008 0.993276 black STRAP -0.430917 0.134484 black SNRNP70 0.097206 -0.089989 black RAB7L1 -0.698623 0.989575 blue PPP3CB -0.095975 0.134352 black GMPPB 0.011209 -0.090219 black PDE1C -0.562751 0.979440 black MRPS24 0.025596 0.134221 black GSR -0.197253 -0.090373 black ARAP1 -0.115285 0.979074 black SF1 0.061707 0.134089 black HSPA4 0.023042 -0.090419 black CEP170 -1.062475 0.965027 blue RPL34 -0.012737 0.133958 black RAI14 0.192320 -0.090649 black CDC123 -0.709127 0.964066 blue RPL10A -0.019039 0.133827 black HNRNPF -0.123481 -0.090649 black GMPR2 1.784671 0.949124 black RPL30 0.037312 0.133432 black AP3S1 -0.024575 -0.090818 black PSAT1 -0.793057 0.940129 blue HARS -0.166422 0.133300 black COPB2 0.122076 -0.091095 black SPG20 -1.002455 0.937495 blue SMARCA5 -0.201576 0.132906 black AHNAK2 -0.672520 -0.091817 black TUBB -0.896442 0.934554 blue PFDN6 -0.039147 0.132511 black CAND1 0.029983 -0.092494 black TUBB4A -0.773097 0.934403 blue RPL7 -0.088255 0.132248 black TRAPPC6B 0.163628 -0.092617 black PCM1 -0.899453 0.927214 blue ACTB 0.034216 0.132116 black LRRFIP1 -0.287642 -0.092694 black IFITM3 -0.321784 0.925164 black PPP1CB 0.024320 0.131853 black UFM1 -0.388733 -0.093094 black CYP51A1 -1.040319 0.924404 blue RFTN1 -0.127400 0.131063 black SMPD4 -0.307484 -0.093217 black BIRC6 -0.696798 0.924328 blue NDUFA10 -0.176609 0.130667 black NDUFAF2 0.110898 -0.093510 black MAP1A -1.127557 0.920827 blue NQO2 -0.464047 0.130272 black DLD 0.175173 -0.093710 black EIF1 -1.169972 0.915406 blue PPAT -0.400663 0.130140 black SYNGR2 0.041804 -0.094018 black NRP1 -0.384885 0.913033 black HARS2 0.152897 0.130008 black MCFD2 -0.002079 -0.094095 black TUBB8 -0.837455 0.912420 blue TRIM33 -0.118570 0.130008 black CPSF7 0.064745 -0.094510 black TUBB6 -0.952825 0.911423 blue HERC4 -0.214173 0.130008 black LRRC59 -0.057377 -0.095081 black RFC1 -0.608936 0.910042 blue RPS19 -0.053269 0.129613 black NSF 0.061845 -0.095327 black ARHGEF2 -0.967278 0.909504 blue MRPS23 -0.302226 0.129349 black DUSP23 -0.215211 -0.095327 black RFC3 -0.758379 0.908659 blue ILK -0.344447 0.129217 black COPA 0.159758 -0.095589 black PBK -0.808134 0.904966 blue TNFAIP8 -0.366281 0.129217 black IDH1 -0.154891 -0.095635 black MOB1A -0.794908 0.901185 blue EHD1 -0.164901 0.128293 black BET1 0.495593 -0.095651 black SMIM12 -1.379507 0.892430 blue RPL23 -0.197269 0.127105 black SACS 0.269512 -0.096160 black TUBB4B -0.855954 0.885965 blue ME1 -0.104542 0.126973 black CAGE1 0.273098 -0.096175 black MTHFD2 -0.926865 0.881273 blue WDR1 0.046561 0.126709 black CUL4B 0.168000 -0.096468 black DPYD -0.857678 0.881038 blue OAT -0.423101 0.126444 black DYSF 0.222928 -0.096514 black BUD31 -0.768322 0.870108 blue SMC2 0.061984 0.125916 black GNG2 -0.372972 -0.096561 black TUBA4A -1.060819 0.868924 blue MRPL13 -0.134350 0.125783 black RAB11FIP5 -0.179923 -0.096638 black PISD -0.995793 0.868845 blue RHOG -0.200150 0.125651 black COX5A -0.173303 -0.096869 black C8orf59 -0.157303 0.867738 black MRPS6 -0.753726 0.125651 black RAD23B -0.056386 -0.097270 black DDX27 -0.662529 0.867659 blue SIGMAR1 -0.067788 0.125519 black SF3A3 0.113834 -0.097903 black TMEM214 -1.045491 0.865206 blue ACTL6A -0.224452 0.125387 black CBR3 0.302173 -0.097934 black PIN4 0.878215 0.865127 black CHMP2A 0.000865 0.125254 black FAM3C -0.040156 -0.098243 black RBM3 -1.387298 0.864572 blue GNB2L1 -0.164593 0.125122 black PNP -0.024810 -0.098614 black CEBPZ -0.998414 0.863938 blue SKP1 -0.412558 0.125122 black PRKDC 0.204892 -0.098969 black TUBB3 -0.907062 0.862987 blue NAA15 0.058109 0.124593 black TARDBP -0.010192 -0.099093 black KIAA0391 -0.941919 0.857185 blue MRE11A 0.124460 0.124328 black APOO 0.251568 -0.099417 black TUBA1A -0.881826 0.856388 blue RPL23A -0.030870 0.124328 black ECI1 -0.001155 -0.099525 black FTSJ3 -1.148993 0.851999 blue EPB41L3 0.127633 0.124063 black 9-Sep -0.056041 -0.099649 black SDAD1 -0.684712 0.848558 blue RHOA 0.019915 0.124063 black NPM1 0.012926 -0.100128 black BAIAP2 -0.687660 0.847356 blue HIGD1A -0.623776 0.123666 black BRP44L -0.053179 -0.100530 black ARHGEF7 -0.650069 0.847035 blue REEP5 -0.234363 0.123401 black DIS3 0.094912 -0.100654 black YTHDF2 -1.590267 0.836490 blue FDPS 0.013069 0.123269 black SLC25A5 0.367371 -0.101056 black MAP1B -1.153800 0.834630 blue PFDN1 -0.307466 0.123004 black GSTM2 -0.167507 -0.101645 black NAT10 -0.579060 0.833092 black TIAL1 -0.112943 0.122871 black ATP1A1 0.063641 -0.101908 black YBX1 -0.903711 0.825541 blue NDUFB7 0.174023 0.122739 black BRCC3 -0.405967 -0.102806 black MAP7D1 -1.456880 0.824971 blue CCDC47 -0.078929 0.122606 black MKKS -0.318506 -0.103178 black NEK7 -0.848219 0.821955 blue TRAPPC3 0.042224 0.122474 black PTBP1 -0.084946 -0.103271 black ARFGAP2 -0.379695 0.821710 black C4orf3 -0.804602 0.122474 black CLTB -0.052970 -0.103472 black UBL5 -1.001674 0.821465 blue DCTN3 -0.101227 0.122341 black CYB5R1 -0.081308 -0.103968 black SMIM15 -0.496570 0.814263 black CCT3 -0.133527 0.121811 black CRYZ 0.051720 -0.104465 black LOX -1.097980 0.814017 blue TBC1D5 0.012497 0.121679 black ACBD3 0.015355 -0.104573 black MYL9 -0.383379 0.808673 black GRHPR -0.006811 0.121546 black RNPEP 0.079566 -0.104697 black MSRB3 -0.527533 0.798839 black FAF2 -0.183392 0.121281 black SLC2A1 0.308827 -0.104992 black HERC2 -0.999683 0.798507 blue DYNLRB1 -0.125305 0.120750 black GDI2 0.039981 -0.105411 black BAZ1B -0.962294 0.798175 blue SERPINE1 -0.769797 0.120750 black SMNDC1 -0.415268 -0.105659 black IFIT5 -0.364143 0.789187 black AP2S1 0.189287 0.120485 black SFXN1 -0.015972 -0.105970 black FIBP -0.419391 0.783415 black CAT -0.425482 0.120485 black RB1 -0.594117 -0.106622 black SDC1 -1.111789 0.783247 blue TMEM33 0.095857 0.120352 black TP53RK -0.137092 -0.106684 black VPS53 -0.221909 0.782912 black NRBP1 -0.234397 0.120352 black PTGES2 -0.055022 -0.106886 black TUBA1B -0.949784 0.782912 blue RPS9 -0.042903 0.120219 black RCC1 0.115033 -0.107275 black AKAP12 -1.125478 0.780814 blue VPS26A 0.020627 0.120086 black RPLP0 -0.079676 -0.107617 black POMP -1.416731 0.779806 blue ITGA5 -0.609420 0.119821 black LTA4H 0.105544 -0.108316 black URB1 0.010637 0.779050 black DEGS1 -0.041625 0.119555 black NDUFS2 0.046701 -0.108348 black NUP188 -0.957972 0.778545 blue MED23 0.042644 0.119290 black FAM114A1 0.184217 -0.108379 black GBF1 -0.737014 0.777788 blue MKI67 -0.482896 0.119290 black PGM1 0.223052 -0.108627 black QKI -0.819827 0.777367 blue COPS5 -0.160798 0.119157 black UPF2 -0.212551 -0.108721 black STK4 -0.488390 0.776188 black LRRC40 -0.273884 0.119157 black IER3IP1 0.192825 -0.108767 black MCMBP -0.542143 0.769856 black ERAP1 -0.334316 0.118891 black AP1B1 -0.000159 -0.108876 black NAA10 -0.514738 0.766044 black MRPL4 -0.139792 0.118094 black SH3BGRL 0.211760 -0.109203 black G3BP1 -0.951317 0.765874 blue GNA11 -0.381461 0.118094 black SNX2 0.013784 -0.109546 black TACC3 -1.629188 0.764091 blue EMP3 -0.237183 0.117961 black TMPPE -0.204083 -0.109826 black ARHGEF10 -0.442026 0.763072 black SRGAP1 -0.289863 0.117562 black ANP32A 0.126180 -0.109904 black CDC27 -0.658766 0.761966 blue CD151 -0.009858 0.117429 black LRBA 0.098285 -0.110090 black DIMT1 -1.427052 0.761200 blue RPL28 0.022758 0.116498 black GLUD1 0.038436 -0.111492 black NUPL1 -0.387185 0.760264 black DYNLL1 -0.041209 0.116099 black KDELR1 0.253747 -0.111867 black ANKRD17 -0.905926 0.758559 blue UQCRH -0.009611 0.115832 black PRDX6 0.075327 -0.112210 black NENF -0.476900 0.756255 black TRIM25 -0.077619 0.115566 black PAFAH1B3 -0.020692 -0.112600 black CSDE1 -1.013596 0.754802 blue ARFGAP1 -0.059480 0.115300 black ARL8B 0.415218 -0.113068 black NUDCD3 -0.512884 0.754460 black SDHA 0.163241 0.115033 black LNP -0.395758 -0.113723 black STRIP2 -0.362993 0.753519 black OCIAD2 -0.113083 0.115033 black DPY30 -0.231295 -0.114129 black RFC5 -0.604191 0.751549 blue ARIH1 -0.314284 0.115033 black TXN 0.136454 -0.114394 black CHMP3 -0.436490 0.748547 black CHORDC1 -0.541240 0.114900 black ATP6V1B2 0.079975 -0.114785 black CNBP -0.711913 0.744764 blue UBA2 -0.271722 0.114767 black COA6 -0.470869 -0.115113 black GNAI3 -0.800928 0.744161 blue RPS4X -0.159637 0.114367 black TCP11L1 -0.063198 -0.115300 black PSMG2 -0.487055 0.743213 black PCYOX1L -0.302938 0.113967 black NDUFA13 0.173895 -0.115832 black SCAF4 -0.805308 0.742782 blue CD2AP -0.554061 0.113834 black ANXA3 0.436482 -0.115863 black MPP5 -1.015316 0.741834 blue RALB 0.150040 0.113434 black PGD 0.029842 -0.116661 black TXLNA -0.881082 0.736821 blue BZW2 -0.190059 0.113300 black AP2A2 0.147046 -0.116770 black EIF1AD -1.280966 0.729705 blue FERMT2 0.000289 0.113167 black SLC25A6 0.235972 -0.117913 black FADS1 -0.781529 0.727528 blue RPS3A -0.081186 0.113034 black RPL39P5 -0.146372 -0.118116 black TBL1XR1 -0.068877 0.727093 black SLC16A1 -0.101227 0.113034 black C7orf55 0.021195 -0.118805 black DUT -0.624710 0.725698 blue PFKM -0.138235 0.112900 black 7-Sep 0.088142 -0.118852 black BCAP29 -0.408646 0.722641 black UQCRC1 -0.083707 0.112367 black NDUFS5 -0.016964 -0.119745 black CGGBP1 -1.252020 0.720979 blue ATIC -0.204798 0.112367 black CALU -0.070012 -0.119840 black CC2D1A -0.355387 0.719227 black TMX1 -0.276048 0.112367 black LGALS1 0.061845 -0.120326 black DST -0.672773 0.715542 blue C1QBP -0.287483 0.112100 black PGAM5 0.163370 -0.120843 black ERBB2IP -0.399007 0.715015 black PTPN9 -0.038140 0.111833 black ATP2B4 0.222310 -0.121126 black POLR2I -1.472329 0.711935 blue KIF5B -0.064963 0.111833 black VAMP7 0.279531 -0.121455 black UBASH3B -0.790235 0.710702 blue EIF4G2 -0.342342 0.111833 black PSMC6 -0.002758 -0.121471 black POLR2L -0.797012 0.708761 blue GNPDA2 -0.155695 0.111699 black NPC1 -0.066125 -0.121628 black RFC4 -0.618318 0.708320 blue CROCC -0.489301 0.111031 black PPP1R13L -0.114925 -0.121675 black EGFR -0.891295 0.708143 blue NT5C2 -0.109374 0.110898 black CCS 0.029842 -0.121942 black TNFRSF12A -0.940672 0.707436 blue LRPPRC -0.208811 0.110898 black CPNE3 -0.015476 -0.122586 black OGFOD1 -0.962182 0.706376 blue MARCKSL1 -0.858227 0.110898 black EML4 -0.304665 -0.122680 black MET -0.025045 0.704341 black MRPS9 -0.204200 0.110764 black LETM1 0.123666 -0.123230 black MCM2 -0.669235 0.699818 blue ELOVL1 -0.411195 0.110764 black RPL10 -0.310468 -0.123293 black SMARCA1 -0.172636 0.698663 black IPO7 -0.061812 0.110363 black HSPD1 0.020200 -0.123355 black PNO1 -1.080759 0.698218 blue SMC1A -0.163268 0.109962 black EMC1 -0.271183 -0.123513 black MCM3 -0.702210 0.696350 blue RBP1 -0.317355 0.109962 black SDHB 0.151599 -0.123560 black DHX37 -1.165758 0.695548 blue TMED1 0.573084 0.109294 black AIMP1 -0.095635 -0.123685 black EHD2 -0.755137 0.692516 blue MARCKS -0.754188 0.109294 black SMDT1 -0.100128 -0.124251 black VPS13C -0.397334 0.692159 black RPS25 -0.059720 0.109160 black PRKRA -0.092571 -0.125069 black FAM129A -0.493804 0.690104 black GOLGA2 -0.067848 0.108892 black CRKL -0.017709 -0.125164 black TSR1 -0.678534 0.687419 blue UBE2H -0.460489 0.108625 black RPL3 0.155749 -0.125337 black UACA -0.586883 0.685267 blue USP24 0.180275 0.108491 black TM9SF3 -0.090834 -0.125966 black RABGAP1 -0.471749 0.684998 black RPS3 -0.089329 0.108491 black EDC4 0.066124 -0.126171 black PKD2 -0.998068 0.683921 blue LYAR 0.271784 0.108357 black SLC35B2 -0.129276 -0.126344 black PKN2 -0.316582 0.680504 black UBE2M -0.159476 0.108357 black NONO -0.226037 -0.126549 black PPP2R1B -1.263300 0.680234 blue CNDP2 -0.344154 0.108357 black TAGLN2 0.175812 -0.128014 black MRPL37 -0.094957 0.678162 black MRPS30 -0.127415 0.108089 black RAB6A 0.269871 -0.128487 black PPP1CC -0.597409 0.676358 blue CD44 -0.395967 0.107956 black XRCC6 0.064193 -0.128976 black POLR1A -0.104946 0.675997 black LSM1 0.128821 0.107688 black BCS1L -0.214073 -0.129339 black UBE4B -0.693015 0.672742 blue RPL32 -0.033688 0.107688 black NUDT5 -0.066397 -0.129497 black DCTPP1 -1.010658 0.671293 blue SCYL2 -0.308019 0.107688 black NDUFS3 -0.202605 -0.130460 black LAMC1 -0.784657 0.670568 blue ECM29 -0.175599 0.107152 black DHRS1 -0.190339 -0.130571 black HDDC2 -0.129955 0.670297 black HSPH1 -0.321604 0.107152 black MAP1S -0.228064 -0.130902 black FBLIM1 -0.666530 0.669934 blue MRPS16 -0.751392 0.106884 black COPG1 0.011353 -0.130966 black PAWR -0.735500 0.665938 blue PRMT1 -0.286251 0.106750 black CAST -0.134762 -0.131408 black MSH6 -0.829921 0.664392 blue DNMBP -0.057797 0.106616 black TMEM245 0.052694 -0.131550 black TOR4A -1.084854 0.661294 blue RPE -0.139156 0.106616 black GNL3 -0.623465 -0.132056 black EMG1 -0.495207 0.657549 black RAB14 -0.093417 0.105946 black GSTM3 0.152768 -0.132183 black MCM4 -0.736268 0.657366 blue TRAM2 0.017209 0.105812 black RBM12B 0.242572 -0.132309 black ISCA1 -0.761311 0.655810 blue RPL9 -0.102016 0.105812 black VKORC1 0.711142 -0.132436 black YAP1 -0.728264 0.654802 blue FAU -0.171222 0.105410 black PRPF40A -0.092202 -0.132720 black NEDD4 -0.053643 0.654069 black PAPSS2 -0.265934 0.105142 black MCTS1 -0.281299 -0.132799 black EIF4EBP2 -1.014849 0.653610 blue ALDH18A1 -0.118069 0.104873 black PLXNA4 0.397693 -0.132831 black GTF2F1 -0.503118 0.652601 black ECI2 -0.181837 0.104873 black GYS1 0.313478 -0.132942 black UXT -0.173628 0.648465 black TIPRL -0.002758 0.104739 black HSD17B10 -0.010018 -0.133242 black PYCR2 -0.716315 0.648281 blue SLC25A13 -0.108705 0.104605 black PIN1 0.015926 -0.133622 black QSOX2 -0.553892 0.648005 black PABPC1 -0.064193 0.104202 black AFG3L2 0.125254 -0.133685 black DRG2 -0.328236 0.643949 black WARS -0.078031 0.103934 black RPS13 -0.108145 -0.133685 black FADD -0.366801 0.643764 black NT5DC1 -0.367583 0.103934 black OCIAD1 -0.159863 -0.133701 black EPG5 -0.402054 0.640066 black GNAQ -0.387676 0.103934 black DIABLO 0.104202 -0.133749 black RABEP1 -0.600774 0.635244 blue GLS 0.301705 0.103665 black TMED9 0.083928 -0.133796 black DNAJC7 -0.332898 0.635151 black LOC728763 -0.059194 0.102994 black GOT2 0.121413 -0.134857 black SPRYD7 -0.478527 0.634965 black SMC4 -0.102481 0.102860 black PDHA1 0.168899 -0.135285 black TMA16 -1.571708 0.634779 blue RPS5 -0.091464 0.102725 black RAB5A -0.129560 -0.135301 black LRSAM1 -0.366020 0.630592 black SKIV2L2 -0.336628 0.102457 black ANXA2 0.068740 -0.135570 black NUP107 -0.285460 0.630126 black EIF3G -0.476518 0.102188 black OGFR 0.404685 -0.137219 black RFC2 -1.153318 0.628914 blue CYFIP1 0.064055 0.102053 black TOMM5 -0.400968 -0.137330 black POLDIP2 -0.973554 0.625925 blue ACTR1A -0.009524 0.102053 black NOP14 -0.506681 -0.137822 black COA4 -0.947000 0.625738 blue RPL27A -0.177425 0.101919 black RBM42 -0.281369 -0.137934 black RABIF -0.326751 0.625177 black LCLAT1 -0.147889 0.101650 black DECR1 0.222434 -0.138632 black DDX18 -1.053434 0.623586 blue CNOT10 -0.230821 0.101516 black USP5 -0.037888 -0.139474 black DCK -0.751538 0.623399 blue PPP1R9B -0.342982 0.101516 black PTGR1 0.356257 -0.139538 black IDI1 -0.652380 0.622087 blue SEC11L3 -0.441556 0.101516 black NUDCD2 0.190172 -0.139569 black VAMP5 -0.545466 0.620774 black RPS18 -0.019770 0.101247 black ACSL3 0.001298 -0.140094 black BYSL -0.761482 0.619742 blue SGTA -0.193107 0.100574 black ADK 0.038436 -0.140555 black ARFGAP3 0.151729 0.618709 black GAK -0.347344 0.100574 black RAB1B -0.130776 -0.141287 black MCM6 -0.622288 0.618239 blue LASP1 0.094371 0.100439 black ERMP1 -0.041640 -0.141828 black -0.824520 0.618051 blue DCTN2 -0.035003 0.100439 black CLTCL1 0.552180 -0.141892 black PDCL3 -0.842051 0.617298 blue CDK18 0.192573 0.100305 black TGOLN2 0.360083 -0.141955 black TUBGCP2 -0.581583 0.616075 black FNTA -0.022830 0.100170 black CEPT1 -0.597278 -0.142513 black API5 -0.493764 0.615793 black HSPA1A 0.096936 0.100036 black PPM1F -0.025206 -0.142656 black HN1L -0.625912 0.614474 blue FLNA -0.060232 0.100036 black USP7 0.142871 -0.142688 black SNAP29 -0.940562 0.613532 blue EIF2S3 -0.248228 0.099766 black ANXA6 0.264837 -0.142927 black CDK4 -1.127778 0.611645 blue NUDT4 -0.186771 0.099497 black CTSC -0.001689 -0.143389 black MCM5 -0.688799 0.606632 blue GLO1 0.074642 0.099363 black CMC2 -0.128125 -0.143389 black POLE3 -1.220749 0.605968 blue GPX8 -0.216351 0.099228 black LYRM5 0.236707 -0.143787 black SEC23B -0.543762 0.604071 black AGPAT3 -0.261898 0.098958 black UTS2 -0.218347 -0.143851 black ARMC6 0.771801 0.603787 black NUP160 0.051163 0.098824 black SMEK1 -0.226139 -0.143899 black DYNC2H1 -0.746858 0.603217 blue BZW1 -0.103379 0.098689 black SNX9 -0.073196 -0.144409 black TIMM17A -1.513131 0.601221 blue DCTN6 0.196481 0.098554 black MPRIP 0.090040 -0.144489 black RPL22L1 -0.773220 0.599032 blue PSMD13 -0.104170 0.098150 black TOLLIP -0.361861 -0.144824 black EXOC6B 0.131985 0.595886 black -0.187181 0.098015 black TMEM167A 0.287472 -0.145462 black NOB1 -1.180888 0.593019 blue MRPL41 -0.091679 0.097880 black TIMM44 -0.056341 -0.145845 black TUBG1 -0.613035 0.591392 blue UFL1 -0.140905 0.097206 black HLA-B -0.066155 -0.145925 black LSM14A -0.879038 0.589572 blue C14orf1 -0.232091 0.097206 black ACOT7 0.111432 -0.146531 black DDX47 -1.048561 0.586308 blue MRPL34 -0.303543 0.097206 black MARS 0.139862 -0.146962 black TRIOBP -0.335171 0.584674 black GSTM1 0.093154 0.097071 black ABHD12 0.372729 -0.146994 black PCK2 -0.274215 0.584097 black VPS28 -0.370956 0.096936 black NDUFA4 -0.071997 -0.147442 black ANAPC5 -0.181509 0.583230 black CSRP1 0.313710 0.096802 black ATP5C1 0.270110 -0.147554 black FAM195B -0.971035 0.578311 black HPRT1 0.131458 0.096802 black PCNP -0.010774 -0.147650 black BAG2 -0.345711 0.572987 black PSMA3 0.127369 0.096397 black FAP 0.192194 -0.147666 black SCOC -0.628876 0.572696 black TRNT1 0.060047 0.096397 black PRKCSH 0.070664 -0.147777 black HMOX2 0.197362 0.569686 black OLA1 -0.243967 0.096262 black PCYT1A -0.113832 -0.148177 black SERF2 -0.984074 0.569102 black EEF1G -0.125777 0.096127 black VAC14 -0.046608 -0.148353 black CMAS -1.072058 0.566474 black HEBP2 0.014498 0.095857 black 8-Sep 0.134221 -0.148369 black DNAJB4 -0.453478 0.565890 black ERC1 -0.186131 0.095857 black NOTCH2 -0.184080 -0.148529 black GOLGA7 -0.660110 0.565305 black HMGB3 -0.382683 0.095857 black PPP1R18 -0.187674 -0.148993 black PLAU -0.861841 0.565110 black NAA38 0.095317 0.095587 black XPNPEP1 -0.154121 -0.149249 black IMPDH2 -0.556118 0.564232 black PEF1 0.113167 0.095047 black HNRNPD 0.133037 -0.149313 black SMARCC1 -0.464206 0.563744 black NEMF -0.243984 0.095047 black ESYT2 -0.146483 -0.149489 black POLRMT -0.055037 0.555718 black TMEM87A 0.281194 0.094777 black PRPF6 0.028569 -0.150401 black MAN2A1 -0.582404 0.553655 black DDX46 -0.182246 0.094641 black SPTAN1 0.139208 -0.150497 black TRAPPC4 -0.183769 0.553262 black PMPCB 0.090311 0.094506 black FKBP11 -0.577982 -0.150849 black C11orf48 -0.352050 0.552967 black HIBCH 0.200002 0.094236 black TMED5 -0.035934 -0.150994 black TUBB2B -0.517172 0.552771 black DRAP1 -0.052162 0.093425 black UGGT1 0.010637 -0.151058 black SMARCAD1 -0.455376 0.550014 black TPM3 0.075464 0.093019 black EMC6 -0.342634 -0.151218 black UNC45A -0.472489 0.549226 black ATOX1 -0.107477 0.093019 black CLPB -0.237660 -0.151490 black SPATS2L -0.976276 0.548930 black EXOC3 0.028993 0.092613 black TCERG1 0.077790 -0.151683 black PSMG1 -0.617477 0.544485 black CASP3 0.049491 0.092478 black C6orf211 0.039560 -0.152147 black OPTN 0.029276 0.544189 black EIF2B4 -0.079935 0.092343 black PRPF4B 0.178619 -0.152163 black ANAPC7 -0.434715 0.543199 black ATAD3A -0.029043 0.091801 black SRSF1 0.065848 -0.152757 black AHCTF1 -0.452728 0.542010 black SHMT2 -0.072847 0.091801 black NTPCR -0.145174 -0.152965 black ASCC3 -0.216284 0.539333 black AGL 0.273934 0.091124 black PDE6D -0.425811 -0.153013 black IKBKAP -0.233837 0.538141 black ME2 -0.093679 0.091124 black CNOT3 -0.506312 -0.153045 black ABCF2 -1.032300 0.538141 black BID -0.052760 0.090989 black DLG1 0.189666 -0.153222 black ATXN2 -0.704583 0.533464 black CAPZA1 -0.061827 0.090853 black CUTA 0.023468 -0.153254 black FRYL -0.286744 0.532267 black VTI1B -0.129024 0.090447 black GNS 0.314407 -0.153286 black ENY2 -0.805787 0.531169 black HN1 -1.249273 0.090447 black PLS3 0.235605 -0.153607 black DYNLT1 -0.476077 0.530071 black EIF1AX -0.129718 0.090176 black PLOD3 0.062122 -0.153735 black MTR -0.242192 0.527271 black EXOSC2 0.090447 0.089905 black PGLS -0.138172 -0.154121 black EIF3H -0.430003 0.526870 black SARM1 -0.265622 0.089769 black ENO1 0.204266 -0.154907 black TXNDC9 -0.206745 0.526570 black RUVBL2 -0.071270 0.089634 black PBLD 0.063779 -0.155277 black NCAPD3 -0.685547 0.525668 black DNAJC3 -0.204416 0.089634 black SUCLG1 -0.313047 -0.157077 black DHX57 -0.146196 0.523562 black NASP -0.351019 0.089634 black PFKL 0.409038 -0.157431 black GOLIM4 -0.790734 0.522357 black SEC11A 0.119954 0.089498 black LUC7L3 0.146525 -0.157576 black CDC23 0.262072 0.520850 black ITPA 0.028993 0.089227 black AARS2 0.287590 -0.157946 black NR2C2AP -0.469270 0.517427 black GLYR1 -0.407364 0.089227 black ADPRHL2 0.279650 -0.159041 black EEF2 -0.641559 0.517024 black ADPGK -0.296704 0.088820 black SNRPD2 0.085153 -0.159492 black ROCK1 -0.108550 0.516318 black LAMTOR2 0.218223 0.088277 black XRCC5 0.039279 -0.159686 black GABARAP -1.219169 0.513794 black PSMA4 0.000433 0.088142 black SUPT6H 0.109026 -0.159766 black MAGED2 -0.793932 0.512379 black ERI3 0.170181 0.088006 black RAP2B -0.035801 -0.159847 black C12orf57 -0.884620 0.511873 black COX15 -0.035653 0.087734 black PABPN1 -0.348152 -0.160137 black KIF2A -0.629791 0.510759 black C14orf166 -0.233515 0.087734 black PSMF1 -0.270295 -0.160153 black ATXN10 -0.683646 0.510759 black PFN1 0.016782 0.087463 black AHCYL1 0.108223 -0.160250 black UBAP2 -1.114910 0.509442 black GPX4 -0.110386 0.087327 black RAN 0.208018 -0.160540 black GEMIN4 -0.358047 0.509341 black APPL1 0.100439 0.087191 black ETFB 0.100978 -0.160572 black LIMCH1 -0.890225 0.508936 black SIN3A -0.038347 0.087191 black HIBADH 0.076696 -0.161201 black FNDC3B -0.783291 0.508733 black TPRG1L -0.463470 0.086920 black FKBP3 0.233520 -0.161863 black NCS1 -0.528199 0.508631 black STAG2 -0.111976 0.086784 black VARS -0.051579 -0.161944 black POLD1 -0.524396 0.503451 black MRPL19 -0.222414 0.086784 black DNASE2 0.157302 -0.162186 black CLASP2 -0.263127 0.503247 black TXN2 0.072312 0.086648 black DYNC1LI2 -0.232888 -0.162380 black AGPS -0.686197 0.502534 black ARMC10 -0.040037 0.086376 black C2orf47 -0.031636 -0.162460 black BET1L -0.691802 0.501617 black XPOT -0.025162 0.086240 black STX5 2.062467 -0.162654 black PYCR1 -0.512905 0.500598 black UBE2D2 -0.572351 0.085833 black MATR3 -0.024913 -0.163494 black CDK2AP1 -0.729435 0.494979 black MICAL1 0.007052 0.085289 black TMSB10 -0.051354 -0.163995 black PVR -0.254909 0.493545 black SNX4 0.228603 0.085153 black LACTB 0.344828 -0.164221 black PDE12 -0.185310 0.493442 black POP1 -0.250200 0.084881 black RAD50 0.356257 -0.164335 black ACOX1 -0.422791 0.493442 black RPS15 -0.074365 0.084745 black CKAP4 0.233029 -0.164529 black MSH2 -0.577875 0.492725 black KPNB1 -0.021658 0.084609 black COPB1 -0.027807 -0.164723 black AARS -0.495308 0.492007 black HSD17B4 -0.170556 0.084473 black MDH1 0.209391 -0.165159 black ARAF -0.489342 0.490467 black B3GALTL -0.243267 0.084473 black BAG3 -0.362863 -0.165370 black SLC1A5 -0.782422 0.488926 black ADNP -0.541051 0.084200 black EDC3 -0.796661 -0.165402 black EIF4E2 -0.349989 0.488515 black PAK2 -0.386016 0.083656 black ACP1 0.080521 -0.165451 black RRM1 -0.576131 0.487486 black PROSC -0.042739 0.083384 black BLVRB 0.095047 -0.165515 black BAG6 -0.290974 0.486663 black CIAPIN1 -0.589507 0.083384 black STOML2 -0.065853 -0.166195 black HGS -0.185015 0.484705 black GIT1 -0.723349 0.083384 black SYNJ2BP -0.005522 -0.166244 black MCM7 -0.675512 0.483261 black RPL10 -0.166341 0.083111 black SLC25A1 0.063779 -0.166648 black PLEKHA5 -0.513028 0.482642 black TRAPPC2L -0.416019 0.083111 black SPTBN1 0.152897 -0.166843 black ZNF259 -0.249256 0.481506 black ABHD14B 0.139862 0.082975 black SNRPF 0.036468 -0.167102 black NUP62 -0.756209 0.480472 black ARL2BP -0.159960 0.082430 black FOCAD -0.088960 -0.167442 black RAB22A 0.002739 0.478713 black GNPNAT1 -0.166519 0.082158 black NAGK -0.008711 -0.167539 black IFI35 -0.418716 0.476434 black CLN6 0.431569 0.081885 black SAP18 -0.215965 -0.168009 black CTNND1 -0.422946 0.475811 black GLS -0.108394 0.081749 black PGRMC1 -0.216066 -0.168269 black BAP18 -0.415634 0.475292 black TCP1 -0.106933 0.081612 black TXNRD1 0.087734 -0.168950 black PRRC2C -0.984074 0.475292 black PSMB1 0.089634 0.081476 black DBI 0.195348 -0.169128 black COX7A2L -0.612064 0.475085 black MTA2 -0.148689 0.081340 black COMMD1 -0.276695 -0.169728 black OGT -0.482251 0.474254 black SPR 0.012640 0.080794 black POLG -0.055426 -0.169793 black KIF1B -0.782869 0.473527 black GET4 -0.186738 0.080794 black TPD52L2 0.246834 -0.170069 black ENAH -0.243079 0.473319 black CNP -0.133179 0.080248 black SFXN3 0.230449 -0.170280 black LSM12 -0.395910 0.472904 black AP3M1 -0.143660 0.079975 black SNRPE 0.054779 -0.170459 black UBE2G1 -0.213889 0.472280 black UQCC 0.023468 0.079702 black KIAA1967 0.029842 -0.170686 black MACF1 -0.362121 0.472072 black EMC8 0.002018 0.079429 black TOMM34 -0.122853 -0.170686 black FGF2 -0.246395 0.470511 black MRPL49 -0.105535 0.079429 black CYB5B -0.530510 -0.170962 black RQCD1 -0.310254 0.470511 black HEATR3 -0.604104 0.079156 black SNRPA 0.121281 -0.171044 black ZNF845 -1.967645 0.467905 black RAP1B 0.084881 0.079020 black SLC30A7 0.073135 -0.171076 black POLR2G -0.439207 0.467488 black MTHFD1 -0.211030 0.079020 black ZRANB2 0.186881 -0.171255 black ZC3HAV1 -0.328073 0.466236 black NDUFA12 0.050188 0.078883 black CCDC58 0.145482 -0.171840 black MOCS2 -0.391248 0.465191 black NCBP2 0.119555 0.078746 black LARS2 -0.048576 -0.171953 black PTRF -0.285759 0.464773 black TIMM50 -0.271897 0.078610 black ATAD1 -0.336847 -0.172148 black HECTD1 -0.600162 0.464459 black CNN3 0.171975 0.078200 black SCRIB -0.295500 -0.172181 black ISG15 -0.277377 0.463936 black RPS12 -0.053464 0.078200 black APMAP 0.050188 -0.172230 black RRBP1 -0.676826 0.463727 black SH3KBP1 -0.122648 0.078200 black SUCLG2 -0.215647 -0.172230 black RAB18 -0.561941 0.463309 black BLMH 0.301822 0.078063 black TMEM258 0.074916 -0.172636 black TMOD3 -0.602482 0.462785 black POLR2H 0.079429 0.077790 black PDS5B 0.073957 -0.172978 black ARHGEF1 -0.380634 0.462471 black BLOC1S1 0.102860 0.077653 black TSNAX 0.118758 -0.173043 black IKBKG -0.068287 0.462157 black AQR 0.059494 0.077516 black CS 0.087870 -0.173466 black SSU72 -0.646248 0.458487 black LYRM4 -0.194097 0.077380 black MYOF 0.168642 -0.173840 black LTN1 -0.445303 0.456701 black RPS21 -0.015228 0.076559 black CLTC 0.266397 -0.173986 black RTN3 -0.105923 0.455649 black EEF1E1 0.093425 0.076012 black RBM14 0.173383 -0.173986 black UBAP2L -0.830178 0.455544 black KIAA1468 0.254110 0.075601 black COASY 0.059771 -0.174621 black SELK 0.555424 0.455439 black QARS -0.069573 0.075601 black UBE2N -0.090987 -0.174898 black CAP2 0.020769 0.455018 black CDK11A 0.043904 0.075464 black LYPLAL1 0.386701 -0.175908 black TAGLN -0.111492 0.454386 black FIS1 -0.067984 0.075190 black ALDOA 0.227495 -0.176316 black NUP35 -0.492464 0.453860 black NME1 -0.146723 0.075190 black MT-CYB 0.316725 -0.176544 black TTC9C -0.168496 0.453228 black ASAP1 -0.009931 0.075053 black DDB1 0.178619 -0.176821 black SRP9 -0.440263 0.453017 black RPL18A -0.145398 0.074916 black ERGIC3 0.481506 -0.177131 black MYBBP1A -0.633479 0.450485 black LRP1 -0.269321 0.074916 black UBA6 0.210139 -0.177131 black RPRD1B 0.039981 0.449957 black ERLIN1 -0.339090 0.074505 black SRRT 0.209890 -0.177767 black ABI1 0.253989 0.449852 black MRPL55 0.247320 0.074368 black ABHD10 0.124990 -0.177833 black RBBP4 -0.570936 0.449852 black RPL15 -0.007406 0.074231 black FAM49B -0.450380 -0.178535 black THG1L -0.072695 0.449641 black ATG7 -0.014368 0.074231 black SNCA 0.427392 -0.178649 black RILPL1 -0.518350 0.448478 black MMS19 -0.036171 0.073957 black CCAR1 -0.092140 -0.178796 black FTSJD2 -0.016468 0.448161 black PSMD7 0.038858 0.073683 black CDKN2A -0.443085 -0.178878 black PDLIM5 -0.572780 0.445091 black MRPL23 -0.007101 0.073546 black RBM12 0.200253 -0.178959 black PDRG1 -0.624532 0.443501 black PYGB 0.125783 0.073272 black RABGGTA 0.223052 -0.179368 black TPT1 -0.436373 0.442121 black EFHD2 -0.204449 0.072998 black COPE -0.154105 -0.179727 black EIF3E -0.357271 0.441271 black CERS2 -0.401349 0.072860 black ACSL1 0.036046 -0.179956 black BUB3 -0.685617 0.440208 black CFL1 -0.031681 0.072723 black SF3B3 0.112367 -0.179989 black MRTO4 -0.234431 0.438719 black KRAS 0.081749 0.071763 black REEP6 -0.010759 -0.180070 black ZFAND1 -0.460707 0.438080 black MRPL44 -0.213387 0.071625 black PRAF2 0.267955 -0.180593 black ANKFY1 0.094777 0.437228 black WDR11 0.145221 0.071351 black SBF1 -0.020223 -0.180675 black ARHGAP29 -0.433506 0.436055 black SRP72 -0.058608 0.071351 black GAMT -0.180315 -0.180790 black CD81 -0.205846 0.435202 black SYNE1 0.047957 0.071213 black C22orf28 0.065159 -0.181199 black RPAP3 -0.773097 0.434028 black COPS7A -0.160540 0.071213 black PTDSS1 0.080248 -0.181444 black PXMP2 -0.430198 0.433494 black ENOPH1 -0.248536 0.071213 black COX7A2 -0.012635 -0.181444 black SMIM11 -0.386204 0.432425 black NAT14 -0.295500 0.071213 black TFAM 0.356257 -0.181477 black TRIP6 -0.389224 0.432211 black RPL8 -0.097023 0.071076 black SNRPA1 0.007483 -0.181657 black PDPK2 -0.324798 0.431356 black CARHSP1 -0.189763 0.071076 black LRRC16B 0.347666 -0.181738 black LNPEP -0.433195 0.431142 black -0.221472 0.070664 black IDH3B 0.157820 -0.181738 black BRK1 -0.339455 0.429857 black PTPN12 0.273337 0.069977 black LMAN1 0.270589 -0.181837 black TOE1 -0.246464 0.428571 black MYLK 0.013927 0.069977 black S100A11 0.367930 -0.181984 black EXOC7 -0.323191 0.427713 black PPP5C -0.087304 0.069977 black MRPS36 -0.063334 -0.182360 black MRPL3 -0.249342 0.426640 black ATP2A2 -0.203269 0.069977 black ANXA1 0.171975 -0.182442 black SUGP2 -0.314230 0.425137 black ARF4 -0.039592 0.069840 black CAND2 -0.414249 -0.182770 black SDF2L1 -0.126754 0.423955 black RPS2 -0.136648 0.069702 black TP53BP1 0.146003 -0.182950 black GIGYF2 -0.619226 0.423202 black AKT1S1 -0.835342 0.069565 black NDUFA8 -0.072619 -0.183015 black GBP1 -0.126738 0.422879 black PPP2R2A -0.030752 0.069290 black CDC42 -0.204632 -0.183343 black FAM114A2 -0.298123 0.422771 black ARPC5 -0.023900 0.069015 black COMMD10 -0.113239 -0.183703 black MAD2L1 -0.582814 0.422771 black RAB8A -0.085405 0.068189 black VCP 0.083520 -0.183998 black ACLY -0.441008 0.422018 black SSB -0.115676 0.068052 black COX6B1 -0.062475 -0.184589 black CDC73 -0.267705 0.421910 black PIP4K2C 0.075053 0.067776 black MRPL24 -0.436295 -0.185064 black TAX1BP3 -0.568217 0.420078 black SNRPG 0.283803 0.067639 black ECHS1 0.182184 -0.185392 black ASAP2 -0.040215 0.419755 black IFNK 0.035483 0.067639 black FAM21C 0.136191 -0.185392 black STUB1 -0.272855 0.418460 black PDCD6IP -0.020062 0.066675 black MYCBP -0.147889 -0.186213 black DDX3X -0.444989 0.418352 black RPS17L -0.176544 0.066675 black PNPT1 -0.035283 -0.186557 black RPA1 -0.206246 0.417380 black NUCB1 -0.306074 0.066399 black MGLL -0.341098 -0.187165 black MRPL47 -0.088362 0.416407 black GPS1 0.079020 0.065710 black ATP5A1 0.270708 -0.187707 black NUP85 -0.129829 0.416299 black NDUFB1 0.163885 0.065434 black PDHX -0.062158 -0.188036 black MINPP1 -0.344465 0.416191 black UQCRFS1 -0.121706 0.065434 black DCTN4 0.001154 -0.188134 black UAP1 -0.155502 0.413052 black SUPT16H -0.326227 0.065021 black FAHD2A -0.091787 -0.188973 black MBLAC2 -0.495898 0.413052 black COL8A1 0.296311 0.064883 black LSM4 -0.362751 -0.189105 black SEL1L -0.187871 0.412077 black RPL7A -0.079554 0.064745 black SLC4A7 0.019915 -0.189137 black YME1L1 -0.413077 0.411209 black PSMB3 0.129349 0.063917 black SLC33A1 0.156785 -0.189253 black FAM65A -1.346335 0.409798 black PLXNB2 0.008630 0.063917 black ACY1 0.123004 -0.189285 black NUP54 -0.337649 0.409690 black RAB34 -0.177980 0.063917 black HYI -0.075672 -0.189351 black TRMT112 -0.725923 0.409472 black LIMA1 0.057554 0.063227 black HP1BP3 -0.116504 -0.189697 black URM1 -0.947362 0.408494 black GSPT1 -0.194344 0.063227 black SF3B14 0.188781 -0.190141 black MRPL9 -0.181477 0.407951 black VCL 0.096532 0.063089 black PRPF19 0.057970 -0.190421 black POLR2E -0.581540 0.407733 black ITGB5 0.051998 0.062951 black EPHX1 -0.344648 -0.190915 black EIF3C -0.215278 0.406537 black DPM1 -0.242738 0.062812 black ME3 0.074231 -0.190964 black EXOC4 -0.222616 0.406101 black PDLIM2 -0.685315 0.062398 black SEC14L2 -0.068529 -0.191541 black NELFCD -0.211832 0.405230 black MRPL27 -0.152131 0.061292 black SUPT5H -0.001097 -0.191640 black NEDD4L -0.200382 0.405121 black UQCRC2 -0.084670 0.061016 black PDIA6 -0.004016 -0.192579 black DERA -0.009219 0.405012 black ARHGAP1 -0.159251 0.061016 black ALDH3A2 -0.096098 -0.193849 black PTMA -0.586038 0.404576 black MRPS2 -0.180953 0.060877 black MYO1E 0.245496 -0.194526 black SRP14 -0.467593 0.403813 black CCDC22 0.132643 0.060739 black RAB2A 0.321582 -0.195137 black SBDS -0.380071 0.403486 black COPS4 -0.140253 0.060739 black DAZAP1 0.141694 -0.195186 black RABGGTB -0.736028 0.403486 black MAP2K2 -0.176218 0.060739 black NOMO2 -0.056491 -0.195269 black CCNH -0.961929 0.402286 black LBR 0.129745 0.060601 black HNRNPUL2 0.107688 -0.195385 black FHOD1 -0.280895 0.401740 black EI24 -0.004320 0.060601 black PDIA3 0.167358 -0.195467 black FASN -0.571236 0.401521 black RAB9A 0.160662 0.060462 black DAD1 0.207018 -0.195566 black TRAPPC5 -0.293660 0.401303 black MRPL22 -0.064978 0.060324 black RDH13 0.105142 -0.196095 black ROCK2 -0.020238 0.399554 black ANP32E 0.035765 0.060186 black MPST 0.112367 -0.197054 black FKBP8 -0.855118 0.398898 black SPCS3 -0.078183 0.060186 black SMU1 0.345283 -0.197137 black DNAJA1 -0.624933 0.396050 black EIF2B2 0.070664 0.059909 black OXSR1 0.137897 -0.197186 black MPP6 -0.244002 0.395940 black DNAJC13 0.011639 0.059909 black LAMP1 0.339365 -0.197335 black GNG12 -0.317319 0.395611 black RCC2 -0.088147 0.059355 black IPO11 -0.084043 -0.197534 black PUS7 -0.172213 0.394295 black LSM2 -0.180463 0.059355 black NIF3L1 0.149910 -0.197666 black PEX11B -0.241339 0.394075 black PPP2R1A -0.235722 0.059217 black KPNA1 -0.143787 -0.197699 black IFT20 -0.278881 0.394075 black DOCK5 -0.095805 0.058940 black EEA1 0.357270 -0.197716 black MYO9B -0.116442 0.391328 black COPZ1 -0.138632 0.058109 black TXNDC12 0.090989 -0.198990 black RAB3A -0.312939 0.390558 black CHUK -0.144648 0.058109 black CNTNAP3 1.227433 -0.199438 black G3BP2 -0.927963 0.390338 black ADRM1 -0.211932 0.057970 black HLA-C -0.135317 -0.199918 black MRPL18 -0.091080 0.390007 black PSMD8 -0.077391 0.057416 black TMCO1 -0.095897 -0.199951 black LACTB2 -0.604433 0.389567 black MAP4K4 -0.104341 0.057138 black PRDX2 0.092343 -0.200183 black USP10 -0.471089 0.387914 black FAM120A -0.251934 0.057000 black MTDH 0.033088 -0.200747 black TOM1L2 -0.438249 0.386590 black PPP2R5E -0.685547 0.056306 black PKM 0.144307 -0.200830 black SNTB2 -0.093386 0.386480 black FABP5 0.135929 0.056029 black NDUFB9 -0.044717 -0.200863 black MYL12A -0.133701 0.386259 black CUL1 0.077516 0.055890 black TTBK2 0.478402 -0.201029 black MT2A -0.360749 0.386259 black SNX1 0.021622 0.055612 black GSTP1 0.207518 -0.201112 black RAB32 -0.199090 0.385818 black CAPNS1 -0.120420 0.055612 black CSNK2A1 0.014498 -0.201825 black SWAP70 -0.037014 0.385707 black RANBP6 -0.253429 0.055473 black TUFM 0.076012 -0.202987 black THEM6 -0.763416 0.385707 black ADD1 -0.242038 0.055335 black TNKS1BP1 -0.003611 -0.203103 black MEST -0.399464 0.385597 black PTPN11 -0.243592 0.055057 black TRIP13 0.250234 -0.203602 black MYO1B -0.156563 0.385486 black CAMK2D 0.068464 0.054779 black GFPT1 0.176067 -0.203751 black STAU1 -0.438425 0.382833 black OSTF1 -0.072437 0.054779 black P4HB 0.207768 -0.204333 black POLDIP3 -0.538849 0.382833 black UQCR10 0.073272 0.054640 black EIF2B5 -0.153864 -0.205447 black SART1 -0.213621 0.382723 black CUL2 0.189287 0.054362 black ALDOC 0.047399 -0.205663 black TBRG4 -0.592745 0.382280 black RPL17 -0.159557 0.054084 black RANBP3 -0.395625 -0.205813 black RWDD1 -0.371030 0.381837 black MRPS22 -0.269773 0.054084 black CYCS 0.248049 -0.205963 black COMMD7 -0.114207 0.381394 black SKIV2L -0.183605 0.053528 black DDT 0.312665 -0.206495 black BRI3BP -0.238766 0.380729 black CENPE 0.073683 0.053389 black HMGB2 0.057554 -0.206512 black CARM1 -0.172766 0.380619 black BAX 0.062674 0.053111 black SEC31A 0.124725 -0.206545 black HEATR2 -0.795133 0.379066 black CDKN2AIPNL -0.053778 0.053111 black IDH3A -0.067138 -0.206812 black LIMS1 -0.244873 0.378734 black COMT -0.187066 0.053111 black MLEC 0.082294 -0.206862 black SON -0.250268 0.377846 black SIL1 0.156397 0.052833 black HMGCL 0.164786 -0.207794 black EEF1A2 -0.243848 0.377512 black STX18 -0.478125 0.052555 black NOL3 0.068602 -0.208461 black DYNLL2 -0.201941 0.376624 black RRAGC 0.155231 0.052277 black PPP1R12A 0.138945 -0.208911 black LAMTOR1 -0.018717 0.376179 black DPYSL2 -0.126659 0.052277 black NDUFB4 0.094371 -0.208911 black HDLBP -0.581929 0.375512 black CDK17 -1.228064 0.052277 black 0.031819 -0.208911 black UBQLN2 -0.859038 0.375401 black RPS6 -0.182442 0.051998 black SEPHS1 -0.214726 -0.208994 black NUDT9 -0.345161 0.373732 black PICALM -0.116692 0.051302 black RCN1 -0.098397 -0.209512 black RBM39 -0.052596 0.373398 black ETFDH 0.245617 0.051024 black PNPO -0.118116 -0.209528 black AKT2 -0.340057 0.373175 black SEC63 -0.028469 0.051024 black TFG 0.349479 -0.210897 black ITGB6 -0.176495 0.371503 black UBA52 -0.073029 0.051024 black NELFB -0.263906 -0.210964 black SAE1 -0.127352 0.370945 black SF3B5 0.054084 0.050885 black ABHD11 0.166972 -0.211481 black CBFB -0.388960 0.370834 black PCBP2 -0.210747 0.050885 black PURB 0.151079 -0.211832 black PTK7 -0.848349 0.370053 black ATP2B1 0.020769 0.050467 black NDUFA11 -0.203701 -0.212133 black ARHGAP18 -0.289334 0.369829 black RPL21 -0.040512 0.050467 black TIMM8A 0.008200 -0.212400 black GLMN -0.630037 0.369271 black ESD -0.062008 0.049910 black DR1 -0.081828 -0.213103 black RFK -0.639220 0.369048 black LIN7C -0.202970 0.049910 black TKT 0.255199 -0.213119 black SYAP1 -0.402493 0.368936 black TAF9 0.011353 0.049352 black C19orf70 0.001586 -0.213655 black TBC1D4 -0.614205 0.368601 black NDUFA2 -0.281193 0.049352 black YARS2 -0.109343 -0.213788 black IGF2R -0.603950 0.368489 black CDV3 -0.090496 0.049213 black UBA3 -0.130223 -0.214073 black DSP -0.574884 0.368154 black PPP4R1 0.031395 0.049073 black UBE2L3 -0.057497 -0.214441 black DDX6 -0.534558 0.368042 black UBLCP1 -0.010730 0.048236 black CANX -0.249445 -0.214625 black CPA4 -0.884194 0.367930 black PTGES3 0.008200 0.047818 black HAGH 0.308594 -0.214659 black STAT1 -0.280089 0.367595 black DSTN -0.078518 0.047259 black MAP2K1 -0.014237 -0.215329 black TCEB2 -0.299010 0.367483 black RPS16 0.001730 0.047120 black STX7 0.001874 -0.215546 black MRPL38 0.018777 0.367147 black EIF4B -0.321946 0.047120 black DHX38 0.157949 -0.216418 black PSME3 -0.607353 0.366924 black LYPLA2 -0.106265 0.046840 black LMAN2 0.138028 -0.217172 black C11orf68 -0.239021 0.366252 black ACTG1 0.041383 0.046701 black MORF4L2 -0.936166 -0.217558 black EIF3M -0.225430 0.366028 black BCAS2 -0.173238 0.046701 black AKR1B1 0.277985 -0.217591 black VPS37A -0.321405 0.364236 black MYH14 0.046840 0.046561 black ABRACL 0.249021 -0.218011 black EDIL3 -0.492383 0.363676 black ATP6V1D -0.379470 0.046421 black UBE2K -0.427013 -0.218263 black TCEB1 -0.292793 0.361768 black RPL11 -0.035594 0.046282 black GBE1 0.396817 -0.218800 black EPM2AIP1 -0.554655 0.361207 black RPL19 -0.173433 0.046142 black XPO1 0.198117 -0.218884 black SLC35A4 -0.312509 0.359634 black AIMP2 0.057831 0.046002 black ATP5F1 0.217727 -0.219018 black RSU1 -0.618052 0.359184 black BLVRA 0.017209 0.045443 black MRPL10 -0.518722 -0.219052 black PARVA -0.280177 0.358059 black HSPE1 0.052416 0.045163 black ERGIC2 0.251689 -0.219455 black TCEA1 -0.018571 0.357833 black DYNC1H1 0.042784 0.045163 black UFD1L -0.587078 -0.219539 black FAM175B 0.151339 0.356933 black PSMA6 0.191942 0.045024 black NDUFA7 -0.106731 -0.219740 black LYRM7 -0.330919 0.356595 black YWHAE 0.047818 0.044884 black WDR75 0.480575 -0.220043 black EPS15 -0.142974 0.356031 black RPS23 -0.084777 0.044884 black NINJ1 2.405203 -0.220144 black EXOSC1 -0.198924 0.354452 black SUCLA2 -0.070663 0.044324 black CD59 0.300182 -0.220816 black GABARAPL2 -0.623821 0.354452 black EIF4G1 -0.240861 0.044324 black SEC61G -0.074684 -0.221270 black UBQLN1 -0.136220 0.354226 black TLN2 0.092478 0.044184 black SLC9A3R1 -0.137616 -0.221388 black MYL6 -0.178943 0.353775 black EXOC8 0.077927 0.044184 black HLA-A 0.751721 -0.221993 black UBTF -0.430314 0.353775 black MRPL50 -0.091249 0.043904 black PRPF8 0.306962 -0.221993 black PPFIBP1 -0.904602 0.353097 black ARF5 0.090582 0.043624 black PKM 0.391218 -0.222195 black PRPS2 -0.269060 0.352984 black NUDT21 -0.028823 0.043624 black PDLIM1 0.302290 -0.222498 black GTF2I -0.719519 0.352984 black UBE3C -0.065687 0.043345 black SPTLC1 -0.119824 -0.222650 black SLC39A14 -0.130018 0.352646 black ACP2 0.388575 0.042924 black USP39 -0.073408 -0.222818 black BCAP31 -0.168933 0.352194 black COPG2 -0.033348 0.042644 black SLC25A22 0.124990 -0.222852 black TIA1 -0.184097 0.350950 black VPS51 -0.370490 0.042364 black PPIA 0.103263 -0.223256 black PLCG1 -0.344428 0.350837 black ARCN1 0.007196 0.042084 black RARS2 -0.196938 -0.223761 black IPO8 -0.421225 0.350045 black UCHL5 -0.283790 0.042084 black RAP2C 0.171847 -0.224284 black PRPS1 -0.131013 0.349931 black IKBKB 0.078200 0.041944 black CD9 0.021906 -0.224469 black PXDN -0.736437 0.349252 black NPLOC4 -0.259461 0.041804 black SEC24B 0.239276 -0.224604 black HSPBP1 -0.339035 0.348799 black GLOD4 0.218595 0.041664 black SRSF11 -0.612946 -0.226156 black SRM -0.472229 0.347666 black APRT -0.114910 0.041523 black ATP5L 0.113034 -0.226527 black MRPL21 -0.018761 0.347325 black MAPK1 -0.026955 0.041383 black GOLGA3 0.377846 -0.226797 black CAV2 -0.098367 0.347099 black NME2 -0.011006 0.041243 black PRPF4 0.061292 -0.226848 black PIK3R4 -0.173759 0.347099 black USP9X 0.130404 0.040542 black PTPLAD1 -0.139458 -0.226848 black SEH1L -0.294632 0.345964 black DUSP3 -0.085068 0.040261 black TGM2 0.181802 -0.227895 black PUM1 -0.732045 0.345737 black GNPDA1 -0.506763 0.040261 black ATP5B 0.159500 -0.227929 black MYL1 -0.087933 0.345396 black CAMK2G 0.107956 0.040121 black ANTXR2 -0.024106 -0.228114 black TBC1D15 -0.247012 0.344601 black GALE -0.013014 0.039981 black SLC25A24 0.347325 -0.228148 black NEK9 -0.552622 0.343692 black BCCIP -0.543720 0.039840 black SF3B4 0.125519 -0.228841 black FAM160B2 0.010207 0.343578 black ATP5EP2 0.348799 0.039560 black TOMM20 -0.191162 -0.228943 black PCID2 -0.274966 0.342782 black CYB5A -0.293005 0.039560 black PLEC 0.031819 -0.228960 black RAC1 -0.368924 0.342555 black ANXA5 0.084200 0.039138 black COX11 0.006047 -0.229213 black GRB2 -0.257407 0.341644 black PSMD3 0.144307 0.038577 black HNRNPM 0.327457 -0.229349 black MRPS34 -0.235875 0.339594 black EXOSC6 -0.086814 0.038577 black PRDX1 0.092072 -0.230313 black EXOSC9 -0.095158 0.339137 black ARPC2 0.122606 0.038296 black NAMPT 0.099363 -0.230685 black PSPH -0.365109 0.339023 black TPMT 0.014927 0.038296 black ZFR 0.056861 -0.230922 black CNN2 -0.151634 0.338909 black MAT2B -0.040987 0.038155 black COX5B -0.085435 -0.231346 black EIF3D -0.246840 0.338795 black RPL35 -0.117866 0.038155 black STMN2 0.297485 -0.231413 black NXF3 -0.239328 0.338339 black PRKACA -0.264079 0.037734 black MINK1 0.097611 -0.232227 black MDN1 -0.663054 0.337882 black ARPC4 0.179638 0.036749 black GSTZ1 -0.030015 -0.233345 black LRRFIP1 -0.422462 0.337311 black RPL6 -0.079935 0.035905 black HNRNPUL1 0.110363 -0.233379 black YBX3 -1.962632 0.336969 black MIEN1 -0.139299 0.035624 black CTSD 0.167101 -0.233430 black GOLGB1 -0.102357 0.336512 black PRRC2A -0.889022 0.035342 black STT3B 0.252295 -0.233888 black VMA21 -0.251659 0.336169 black CORO1B 0.074231 0.035202 black PTK2 -0.189631 -0.234669 black SPECC1 -0.159396 0.336055 black NAPA -0.125541 0.034920 black MBOAT7 0.196733 -0.235280 black STAM2 -0.289633 0.335598 black RPL14 -0.004769 0.034638 black MRPS18B -0.452945 -0.235688 black NCDN -0.651224 0.335598 black MON2 0.212881 0.034497 black GPNMB 0.997834 -0.236333 black NPM3 -0.456583 0.334911 black BPNT1 0.084336 0.033934 black COG7 0.154972 -0.237286 black ATG3 -0.013407 0.334568 black BDH2 0.380175 0.033511 black HNRNPH1 -0.015841 -0.237864 black MRPL17 -0.279687 0.334225 black RPP14 0.060877 0.033370 black BCLAF1 0.113167 -0.238749 black MPHOSPH6 -0.029220 0.333538 black DOCK7 -0.129118 0.033370 black APOA1BP -0.029249 -0.239072 black EXOC2 -0.031695 0.333538 black RPS15A -0.216585 0.033370 black 15-Sep 0.039419 -0.239123 black DDX19A -0.267184 0.333195 black NDC1 -0.119542 0.033229 black MIA3 -0.208061 -0.239328 black GNAI2 -0.183261 0.332278 black SRPK1 -0.153543 0.033088 black PPCS -0.093679 -0.239464 black CDK5RAP3 -0.344264 0.331705 black LDHB 0.014927 0.032806 black B2M 0.220701 -0.239532 black PPP1R14B -0.563199 0.330903 black PSMA1 0.075875 0.032665 black SHC1 -0.198808 -0.241168 black DFFA -0.117553 0.330329 black APEH -0.137045 0.032524 black FKBP7 0.172231 -0.241219 black SPATS2 -0.514759 0.330214 black MRPS25 -0.142465 0.032524 black NUCKS1 0.826518 -0.241970 black IPO5 -0.231058 0.329640 black PON2 0.071763 0.032383 black CNPY2 0.190425 -0.242004 black CISD1 -0.236503 0.329066 black KARS -0.048800 0.032383 black SRSF6 0.301471 -0.242413 black C11orf73 -0.394449 0.328836 black MRPS17 -0.215597 0.032383 black BSG -0.028219 -0.242431 black DDA1 -0.742362 0.327687 black RBM25 -0.103193 0.032101 black GLTP 0.117562 -0.242482 black PDCD10 -0.160508 0.326307 black HEBP1 -0.134810 0.032101 black GLRX5 -0.005131 -0.242499 black EIF3F -0.226983 0.325962 black MRC2 -0.167588 0.032101 black ORC3 -0.214793 -0.242738 black CAD -0.305931 0.325962 black LRPAP1 0.181675 0.031960 black SEC24C -0.055936 -0.243318 black PDZD11 -0.468411 0.325962 black AHCY -0.030118 0.031960 black UBA1 0.142740 -0.243916 black EIF4A1 -0.262521 0.325041 black EIF5B -0.249977 0.031960 black SUMO1 0.706818 -0.244070 black TFRC -0.428119 0.324695 black IFT27 -0.205147 0.031819 black ATP5D 0.082566 -0.244634 black HSPA8 -0.292917 0.324350 black RHOA -0.271200 0.031819 black WDR18 0.084473 -0.244685 black C10orf32 -0.186754 0.322851 black RUVBL1 -0.047488 0.031677 black RALA 0.288299 -0.244822 black NUDC -0.482432 0.322505 black DNAJC10 0.150820 0.031536 black SNRNP200 0.301002 -0.245010 black TRAPPC1 -0.185835 0.322390 black ARHGAP17 0.003170 0.031536 black IDH2 0.398460 -0.245147 black ALDH1B1 0.026021 0.322159 black RAB5C -0.123465 0.031395 black UPF1 0.154324 -0.245215 black UBE2A -1.156048 0.321351 black SSR1 -0.209261 0.031395 black RHOC 0.113434 -0.245215 black VPS25 -0.357086 0.321235 black NIT2 -0.078091 0.030972 black SART3 0.314871 -0.245643 black MST4 -0.484025 0.321120 black LARS 0.049352 0.030689 black SERPINB8 0.374622 -0.245797 black DHCR7 0.064469 0.319734 black TRRAP -0.100917 0.030689 black VAT1 -0.256632 -0.245968 black PACS1 -0.260031 0.319618 black SETD7 -0.185819 0.030689 black RASA1 0.437547 -0.246635 black PAICS -0.299543 0.319387 black HNRNPA0 0.041804 0.030548 black PSAP 0.175684 -0.246635 black NUP93 0.043204 0.319040 black AP3B1 0.124725 0.030407 black YIPF5 0.145090 -0.247029 black RAPH1 -0.539918 0.318924 black MRPS28 0.009634 0.030407 black GNL1 0.089227 -0.247577 black FDX1 -0.108596 0.317536 black NDUFV2 -0.124377 0.030407 black COX20 0.211386 -0.247748 black CIRBP -0.372617 0.316146 black CISD2 -0.148657 0.030407 black ZC3H18 -0.433993 -0.247868 black ATXN7L3B -1.414922 0.315450 black BROX 0.070252 0.030124 black CWF19L1 -0.443105 -0.248262 black ABCF3 -0.511938 0.314755 black RPL13A -0.052596 0.029983 black NEXN 0.247806 -0.248279 black IRF3 -0.182033 0.314639 black ATP13A1 -0.028190 0.029559 black FAHD1 0.092884 -0.248279 black RNASEH2C -0.514367 0.312897 black ATP1B3 -0.144632 0.029418 black ATP6V0D1 0.140255 -0.248845 black CTNNB1 -0.367453 0.311852 black MOV10 -0.005160 0.028711 black C21orf33 0.033652 -0.249016 black SUGT1 -0.484570 0.311619 black UBFD1 0.031536 0.028428 black COLGALT1 0.002595 -0.250303 black PAM16 -0.115441 0.311271 black SRA1 -0.934014 0.028286 black FKBP14 -0.628809 -0.251109 black RPF2 -0.050861 0.311154 black EIF2A -0.100020 0.028145 black UCHL3 -0.133575 -0.251487 black FLOT2 0.088549 0.310456 black HBS1L -0.193057 0.028145 black DARS2 0.450697 -0.252226 black CENPF -0.041091 0.310224 black THUMPD3 -0.483884 0.028145 black ASPH 0.094101 -0.252432 black RANBP2 -0.291557 0.309758 black ZNF292 -0.398437 0.028003 black EPS8 -0.146196 -0.252948 black NME7 0.283448 0.308361 black PUF60 -0.203020 0.027720 black TMED10 0.208268 -0.253292 black CTNNA1 -0.385940 0.307662 black DYNC1LI1 0.028569 0.027437 black TOP2A -0.202871 -0.253825 black FAM136A -0.139172 0.307312 black UBE2V1 -0.100252 0.027154 black EIF4A3 0.314755 -0.254100 black SNX17 -1.006739 0.306612 black PGM5 0.172488 0.026588 black LONP1 -0.018878 -0.254186 black PPP3R1 -0.144808 0.306496 black CTBP1 0.059217 0.026446 black EFTUD2 0.249627 -0.254513 black RCN2 -0.362807 0.305329 black MAP4 -0.348133 0.026304 black LDHA 0.429321 -0.254771 black SLIRP -0.454032 0.302875 black PIK3C3 -0.071012 0.026021 black RPL22 -0.200498 -0.254857 black MAT2A -0.638568 0.302173 black EIF2S1 -0.162848 0.025171 black SERPINB6 0.306729 -0.254892 black DAP3 -0.379357 0.301354 black UROD -0.411867 0.025029 black CLPTM1L -0.223290 -0.255064 black EIF1B -0.113411 0.300299 black MRPS27 -0.379545 0.024320 black C9orf142 -0.098722 -0.255098 black AKT1 -0.165192 0.300299 black FKBP9 0.057277 0.024036 black FLNB 0.150820 -0.255736 black PGP 0.119821 0.299714 black PSMD6 -0.010934 0.024036 black IVD 0.095182 -0.256028 black TIMM13 -0.321802 0.299714 black NQO1 -0.070058 0.023894 black MRPL40 -0.304166 -0.256476 black CACYBP -0.269651 0.299127 black FAM160B1 -0.084426 0.023894 black SEC22B 0.257011 -0.256649 black WDR77 -0.022493 0.298541 black UGP2 -0.002946 0.023610 black SAMD9 0.477366 -0.256752 black SLC7A5 -0.446207 0.298306 black RDH11 0.048794 0.023468 black MYO6 0.388245 -0.257373 black EDF1 -0.699513 0.298306 black PSMD2 0.012640 0.023042 black PPA2 -0.086370 -0.259426 black LUZP1 -0.413596 0.297250 black RNH1 0.006908 0.023042 black MRRF -0.094973 -0.259582 black NCAPD2 -0.333589 0.296428 black RPS10 -0.003033 0.022900 black DGKA 0.195222 -0.259824 black GAPVD1 -0.239021 0.296193 black HDAC2 -0.347803 0.022758 black NUDT1 0.519139 -0.260325 black GIPC2 -0.297094 0.296193 black PNMA2 -0.179711 0.022616 black RANGAP1 0.416407 -0.260601 black ST13 -0.160605 0.295958 black SMCHD1 -0.223845 0.022616 black FN1 -0.073166 -0.260705 black TAB1 -0.496061 0.295841 black PHKB -0.186722 0.022474 black MANBAL -0.623176 -0.261258 black DCBLD2 -0.907657 0.295723 black NDUFB6 -0.017607 0.021764 black MT-CO2 -0.032153 -0.261760 black DHX30 -0.543089 0.295605 black ITGA11 -0.199206 0.021053 black S100A13 0.190299 -0.264339 black RAB3B -0.210546 0.294782 black YRDC -0.678257 0.020911 black NHP2 -0.176169 -0.264495 black PPP6C -0.169290 0.293606 black NCLN -0.140619 0.020769 black ATP5O 0.150040 -0.264582 black STAM -0.312832 0.293488 black DPAGT1 0.293370 0.020627 black ATP5H 0.179766 -0.265431 black EIF3K -0.139379 0.293135 black COPS8 -0.133638 0.020342 black ATP5J2 0.174023 -0.265570 black MRPL30 -0.159283 0.293017 black DYNC1I2 -0.309467 0.020342 black EMC7 -0.468990 -0.265622 black NELFA -0.691150 0.292546 black VAPB -0.236571 0.020200 black SYPL1 0.293606 -0.265900 black TRIO -0.216837 0.292428 black STXBP1 -0.098258 0.020058 black GORASP2 0.182946 -0.266368 black C16orf80 -0.251144 0.291957 black NUDT2 0.032524 0.019773 black TMX4 -0.303792 -0.266368 black YTHDF3 -0.547489 0.291839 black DDX42 -0.118931 0.019204 black KIAA1429 0.036327 -0.266576 black PPP4C -0.328417 0.291250 black PSMD12 0.039981 0.018919 black LUC7L 0.409255 -0.266698 black ZW10 0.038717 0.290424 black PSMD4 0.104202 0.018777 black SUPV3L1 0.279650 -0.267653 black FEN1 -0.695349 0.289480 black MRPS18A -0.542353 0.018634 black DNAJC5 0.123931 -0.267861 black EXOSC3 -0.226561 0.287945 black GMFB 0.033370 0.018064 black PURA 0.193204 -0.268487 black ATL3 -0.251367 0.287354 black MRPL53 -0.182491 0.018064 black MDH2 0.088413 -0.268973 black PSMG4 0.264116 0.286999 black PGRMC2 -0.092202 0.017922 black PRDX5 0.079839 -0.268991 black FUT8 -0.528240 0.285935 black FXN -0.050368 0.017779 black HDGF 0.043204 -0.269443 black GOSR1 0.241596 0.285580 black RPL36 -0.097162 0.017209 black KIF4A 0.115566 -0.269495 black XPO6 -0.125919 0.284988 black CSTB -0.155566 0.016924 black INPP4B -0.208678 -0.269564 black ZNF830 0.223546 0.284751 black ANO10 -0.028087 0.016782 black COX14 -0.058383 -0.269808 black TRIM26 -0.087120 0.284396 black PYGL 0.391548 0.016496 black NDUFA3 -0.511506 -0.270086 black PEA15 -0.156113 0.284040 black COPS2 0.039700 0.016496 black TECR 0.121811 -0.271357 black DHFR -0.183703 0.284040 black ADH5 0.177726 0.016354 black GUK1 0.352194 -0.272019 black POLR2D -0.047309 0.282973 black FLOT1 0.074094 0.016211 black PRCP 0.194717 -0.272141 black NOP16 -0.058067 0.282973 black KDELC2 -0.106964 0.016211 black JAGN1 0.217851 -0.272855 black EPHA2 -0.388053 0.282855 black COPS6 -0.108892 0.015926 black HSPA12A 0.238053 -0.273814 black MRPS18C 0.121546 0.282618 black DOHH -0.276415 0.015355 black PLBD2 0.353210 -0.274512 black INIP -0.407134 0.281431 black NF2 -0.233464 0.015212 black NISCH 0.251568 -0.274739 black BTF3 -0.825389 0.281431 black DENR -0.091940 0.015069 black SQRDL 0.300534 -0.274791 black GART -0.444164 0.281312 black PPP2R3A 0.067501 0.014927 black VAMP3 0.114234 -0.275053 black SNAP23 0.023042 0.280956 black PITPNA -0.043289 0.014927 black RPS27L -0.114363 -0.275123 black EIF3J -0.525413 0.280600 black ATP6V1G1 0.184598 0.014784 black GSTO1 0.198620 -0.275489 black DNAJC8 -0.245027 0.280362 black KRT18 0.243913 0.014498 black SEC61A1 -0.053718 -0.276136 black EXOSC5 -0.080484 0.279650 black TPR 0.002450 0.014498 black ZNF326 0.080112 -0.276922 black TTLL12 -0.263906 0.278698 black NDUFS1 -0.125321 0.014498 black SP100 0.663117 -0.277936 black LAMTOR4 -0.053613 0.278580 black OSBPL8 -0.320180 0.014498 black ARHGDIB 0.269991 -0.278094 black RTN4 -0.164335 0.278342 black PFKP 0.165044 0.014355 black PPIF 0.423417 -0.278986 black MARVELD1 -0.487055 0.278223 black NAP1L4 -0.270956 0.014355 black NPEPPS 0.233520 -0.279687 black PRKAR1A -0.365444 0.278104 black PBDC1 -0.500708 0.014070 black DNAH14 0.012926 -0.280019 black GSDMD -0.053808 0.277985 black FAM96B -0.312384 0.013927 black NDUFC2 0.006908 -0.280054 black RPA3 -0.184064 0.277985 black GGCT -0.328852 0.013784 black LMF2 0.240375 -0.280562 black CUL4A -0.178910 0.277747 black ERP29 -0.168933 0.013641 black IARS2 0.202386 -0.281018 black HS2ST1 -0.624599 0.277509 black DNM1L -0.069058 0.013498 black MIF 0.175684 -0.281176 black COMMD2 -0.095712 0.277271 black IGF2BP2 0.135404 0.013069 black DCUN1D5 0.133695 -0.281176 black XPO4 -0.516326 0.276437 black LARP1 -0.454210 0.013069 black B3GAT3 -0.319532 -0.281229 black PSMB10 0.004897 0.276318 black ELOVL5 -0.053494 0.012926 black MAVS -0.514656 -0.281281 black ETF1 -0.154297 0.275841 black IARS 0.012068 0.012354 black NIT1 0.248292 -0.281351 black CLPX -0.438210 0.275841 black FLNC -0.216501 0.012211 black ERGIC1 0.092749 -0.281562 black RAB21 -0.201228 0.275365 black GBAS 0.170053 0.012068 black RPN1 0.193456 -0.281632 black NBAS -0.017972 0.275007 black FSCN1 0.136716 0.011782 black SLC35E1 0.049352 -0.281877 black NUP155 -0.342360 0.275007 black RBX1 -0.192629 0.011639 black PELP1 0.253626 -0.282158 black EOGT -0.242004 0.274649 black CSNK2A2 -0.013130 0.011353 black IMPA1 0.249021 -0.282228 black WDR82 -0.036999 0.274530 black MAPK15 0.022190 0.011209 black TMED2 0.350610 -0.282491 black PPP3CA -0.121738 0.274053 black RPL27 -0.075687 0.010923 black GAPDH 0.174151 -0.282614 black SRXN1 -0.126281 0.274053 black SMARCC2 -0.020428 0.010780 black SF3A1 0.070801 -0.283720 black GOLPH3 -0.559367 0.274053 black UBXN1 -0.324509 0.010637 black ACO1 0.231678 -0.283826 black PCBD1 0.269153 0.273337 black ACTR3 0.120750 0.010207 black PLD3 0.126180 -0.285390 black GLIPR2 0.038155 0.273337 black CHERP 0.140910 0.009920 black ACADSB 0.220454 -0.285952 black NAA50 -0.543720 0.273217 black TMEM106B -0.431793 0.009920 black SLC25A3 0.295841 -0.286181 black FNDC3A -0.118868 0.273098 black COTL1 0.179001 0.009634 black SCARB2 0.173511 -0.286568 black RIC8A -0.224958 0.272262 black PCBP1 -0.080942 0.009634 black AK4 0.676538 -0.286991 black UBE2R2 -0.480277 0.272262 black TNS3 -0.295730 0.009634 black FKBP2 0.124725 -0.288188 black BCAT1 -0.328670 0.271904 black NCL 0.073272 0.009491 black NANS 0.135929 -0.288576 black PDLIM7 -0.291186 0.270947 black VPS4B 0.062951 0.009491 black AKAP8 0.209641 -0.289298 black TTC38 -1.002281 0.270828 black ANP32B -0.080103 0.009347 black HNRNPA1 0.239887 -0.289827 black GEMIN5 -0.470109 0.270469 black AP2B1 0.051442 0.009204 black PPME1 0.085425 -0.290145 black PDS5A -0.381085 0.269392 black ATP6V1A 0.085561 0.008774 black CBX3 0.012354 -0.290692 black EIF4EBP1 -0.255426 0.269273 black ARPC3 0.065572 0.008774 black SNRNP40 0.032242 -0.290798 black NOLC1 -0.834493 0.268794 black DPCD -0.179645 0.008774 black LMNB2 0.327687 -0.290939 black MRPL20 -0.306306 0.268315 black RPS15A -0.011646 0.008630 black ENSA -0.384640 -0.291610 black DNAJC9 -0.411291 0.268195 black SH3BGRL3 0.072998 0.008343 black SEC23IP 0.231801 -0.291963 black LAMTOR5 -0.321459 0.267116 black DLAT -0.037636 0.008057 black ALYREF 0.249870 -0.293306 black SPECC1L -0.421090 0.266637 black NT5E 0.088413 0.006908 black INTS3 0.007339 -0.293324 black SFT2D2 -0.833619 0.266517 black TOR1AIP2 -0.602547 0.006621 black SFPQ -0.111633 -0.293819 black NRD1 -0.445107 0.265797 black NDUFB3 -0.011719 0.005903 black COX6C 0.146003 -0.294155 black BCAR1 -0.637177 0.265197 black PSMB2 0.060186 0.005616 black PLOD1 0.213254 -0.294509 black GOPC -0.571815 0.264596 black 11-Sep 0.116631 0.005328 black PAFAH1B2 0.163756 -0.294845 black TRAP1 -0.304309 0.263756 black RPS8 -0.045059 0.005328 black EBP 0.580724 -0.295040 black CTPS1 -0.290339 0.263635 black GTF3C1 -0.149953 0.005328 black STT3A 0.227495 -0.295305 black MT1H -0.495919 0.263275 black HADH -0.026881 0.005184 black CTSB 0.084473 -0.295394 black QRICH1 0.060462 0.262553 black AHNAK -0.199868 0.005184 black PWP1 0.148479 -0.295819 black ASCC2 -0.099201 0.262072 black C12orf10 -0.241612 0.005184 black CLIP1 0.191815 -0.295889 black HSP90AB2P -0.305200 0.262072 black HDHD2 -0.024883 0.005041 black PHF5A 0.155490 -0.296563 black EIF2B3 -0.092309 0.261952 black TRAM1 0.314871 0.004753 black ACOT1 -0.066971 -0.297201 black RNF20 -0.176903 0.261952 black NAE1 -0.016454 0.004753 black GGH 0.400866 -0.297467 black PHLDB1 -0.955508 0.261832 black APEX1 -0.333916 0.004753 black MYH10 0.373398 -0.297910 black KPNA4 -0.165095 0.261350 black PPM1G -0.050234 0.004609 black HDGFRP3 0.156785 -0.298336 black GTF2F2 -0.170784 0.260748 black MAPK14 -0.169209 0.004322 black YES1 0.099093 -0.299489 black VPS45 -0.325340 0.260748 black SEC62 -0.191986 0.004322 black AIFM1 0.140255 -0.300129 black EZR -0.178976 0.260026 black RABL6 -0.451583 0.004178 black SRRM1 -0.117005 -0.300360 black ARPC1A 0.202136 0.259423 black ACSL4 -0.198659 0.004034 black MDC1 0.187641 -0.300679 black DHX36 -0.489889 0.259182 black ABCF1 -0.101026 0.003890 black SEC24D 0.320889 -0.300910 black NDUFA9 -0.102186 0.258941 black EEF1B2 -0.267253 0.003890 black LSM7 0.168000 -0.301301 black CAB39 -0.119307 0.258820 black SNRPC 0.028569 0.003746 black TMEM70 -0.354188 -0.301764 black ARMC9 -0.254135 0.258338 black SCRN1 -0.099572 0.003602 black RNMT 0.289834 -0.301995 black EXOSC10 -0.100577 0.258097 black PSME1 0.028569 0.003458 black HSPA4L 0.025171 -0.303169 black TPM4 0.002306 0.256407 black IST1 -0.080866 0.003314 black IKBIP 0.611078 -0.303312 black TJP1 -0.339473 0.255924 black RGS10 -0.505226 0.003026 black TSPO 0.361095 -0.304434 black STX16 -0.464843 0.255924 black PSMB4 0.131458 0.002883 black RER1 0.185867 -0.305129 black DNAJB1 -0.502383 0.255803 black MRPS35 -0.152083 0.002450 black MRPL16 -0.682742 -0.305218 black SH3GLB2 -0.011210 0.254957 black FARSB -0.121000 0.002306 black THUMPD1 0.301822 -0.305467 black VPS33A -0.343787 0.254957 black AGFG1 -0.272210 0.002162 black CMC1 -0.136315 -0.305788 black PTP4A2 -0.532554 0.254473 black PSMA7 -0.002296 0.001586 black ATP5G1 0.438080 -0.306930 black STK24 -0.231024 0.254352 black CMBL 0.276794 0.001298 black BTF3L4 0.065434 -0.307037 black GNB1 -0.163236 0.254231 black TTN 0.199876 0.000577 black MCCC2 0.195348 -0.307126 black SELT -0.242840 0.254231 black UGDH -0.140460 0.000577 black ETFA -0.018337 -0.307359 black APPL2 -0.037414 0.253263 black TALDO1 0.089363 0.000433 black SEC24A 0.084473 -0.307644 black MRPL39 0.017637 0.252779 black GMPPA -0.349842 0.000144 black NIPSNAP1 0.245861 -0.307894 black SCP2 -0.305949 0.252295 black FLNA -0.424513 0.000000 black PLP2 0.188401 -0.309377 black COMMD8 -0.244053 0.251204 black DHRS4 -0.098845 -0.000332 black TNPO2 0.240986 -0.310593 black ARMC1 -0.363271 0.251204 black PCYT2 -0.284669 -0.000418 black SNRPD1 0.120617 -0.311238 black SOAT1 -0.147538 0.250962 black M6PR -0.047294 -0.000447 black SCPEP1 0.409581 -0.311291 black METAP2 -0.431481 0.250962 black TP53I3 0.288772 -0.000476 black GRN 0.044324 -0.312993 black BRIX1 -0.320666 0.250840 black ITPR3 -0.157190 -0.000794 black IQGAP1 0.255319 -0.313369 black NUBP2 -0.626201 0.250719 black PXN -0.119322 -0.000808 black EFTUD1 0.333195 -0.313441 black RBMS1 -1.185770 0.250477 black FKBP1A 0.149910 -0.001111 black PDPR 0.238542 -0.314948 black YWHAH -0.227658 0.249991 black ACTC1 -0.099974 -0.002036 black CNPY4 -0.047681 -0.315127 black GTF2A2 -0.129276 0.249870 black RPS14 -0.159057 -0.002238 black AK2 -0.119949 -0.315433 black TBCE -0.070118 0.249749 black DNAJC19 0.010780 -0.002339 black SURF4 0.418784 -0.316187 black IRGQ -0.106933 0.249627 black GMPR -0.066760 -0.002802 black LAMTOR3 0.056029 -0.316726 black USP47 -0.248656 0.249627 black HSDL2 0.020627 -0.003004 black SNRPN 0.293370 -0.318452 black PTPN1 -0.403504 0.249627 black ECE1 -0.211314 -0.003105 black ETHE1 0.091801 -0.318848 black SRP19 -0.272384 0.249506 black RPL18 -0.059074 -0.003192 black MT-ND5 0.351854 -0.319100 black STAT2 -0.779695 0.249385 black CLIC1 -0.106778 -0.003336 black HTT 0.285580 -0.319514 black KCMF1 -0.067893 0.249021 black RPSA -0.034013 -0.003611 black RPN2 0.150170 -0.320216 black VPS4A -0.508198 0.248292 black NNMT 0.267476 -0.003785 black CPOX 0.041664 -0.320756 black EIF3I -0.360916 0.248049 black KHSRP -0.298761 -0.004248 black NNT 0.252416 -0.320846 black TLN1 -0.168706 0.247441 black DDX5 -0.230939 -0.004465 black U2AF2 0.091801 -0.321441 black SLK -0.174279 0.246834 black NDUFC1 0.137897 -0.004682 black GDI1 -0.061059 -0.322199 black METTL1 -0.357492 0.246226 black YWHAG 0.062951 -0.004682 black IKBIP 0.470927 -0.322650 black SLFN5 -0.544098 0.245982 black POFUT1 -0.283369 -0.004740 black SLC16A3 0.174406 -0.323299 black RBM26 -0.260739 0.245252 black RPS7 -0.268452 -0.004754 black GALNS 0.773659 -0.323372 black USP8 -0.564329 0.244887 black PIGS 0.099766 -0.005131 black TEX10 0.030265 -0.324022 black ECHDC1 -0.330048 0.244765 black TMEM97 -0.502056 -0.005160 black ATP5J 0.040822 -0.325738 black SARNP -0.337503 0.244644 black MSN 0.168642 -0.005536 black SND1 0.203640 -0.326009 black BOLA2 -0.341354 0.244522 black UQCRB 0.122739 -0.005551 black NPC2 -0.013640 -0.326353 black SVIL -0.475555 0.243791 black TM9SF4 -0.077970 -0.005623 black AAK1 -0.070179 -0.327819 black PAPSS1 -0.480458 0.243669 black PRPSAP1 -0.110931 -0.005638 black FKBP10 -0.240179 -0.327910 black RRAS2 -0.058924 0.243425 black PSMC5 -0.048785 -0.005869 black HINT2 0.048655 -0.328055 black TMEM43 -0.138203 0.243182 black FASTKD2 -0.759405 -0.006101 black ISOC2 0.235972 -0.328381 black MRPS7 -0.745769 0.242450 black TRIM32 0.260748 -0.006318 black DKC1 0.022900 -0.328399 black XPO5 -0.400111 0.242084 black HLA-A -0.218833 -0.006507 black PCMT1 0.404358 -0.330066 black PALLD -0.316223 0.241962 black DHX15 0.092207 -0.006652 black ATPIF1 0.524666 -0.330737 black STAT6 -0.251659 0.241596 black PODXL 0.085833 -0.006826 black SVIP -0.244702 -0.331191 black RAB11B -0.136014 0.241108 black PSMB6 0.138159 -0.007246 black OXCT1 0.359521 -0.331373 black FKBP4 -0.228520 0.240986 black RAB27B -0.044896 -0.007261 black SLC39A7 0.045024 -0.331681 black ATXN2L -1.035934 0.240864 black DNM2 0.239031 -0.007406 black DCXR 0.067226 -0.332335 black ISOC1 -0.824009 0.240620 black PPP2R5C -0.026808 -0.007942 black AGTRAP 0.407298 -0.332589 black NUP205 -0.131155 0.240253 black VIMP -0.809145 -0.008290 black APP 0.319387 -0.333262 black ARL1 -0.022771 0.240009 black ALG2 -0.326679 -0.008305 black PEBP1 0.230080 -0.334989 black RPS29 -0.117787 0.239765 black IRGC 0.334683 -0.008421 black OPA1 0.118626 -0.335390 black CCDC124 -0.482392 0.239154 black CLINT1 -0.006145 -0.008450 black HK2 0.287118 -0.335827 black DEK -0.162057 0.239031 black PFDN4 -0.122994 -0.008697 black KRTCAP2 -0.013043 -0.336683 black FTO -0.484510 0.238298 black TBL2 0.003314 -0.008958 black PDP1 0.081612 -0.337047 black DHRSX -0.061812 0.238175 black RANBP1 -0.235994 -0.009176 black NUTF2 0.219215 -0.337193 black PSME4 -0.222734 0.238175 black DBNL -0.152869 -0.009336 black BASP1 -0.221001 -0.338068 black ATP1B1 -0.766652 0.237686 black MRPL15 -0.434929 -0.009684 black HNRNPL 0.290306 -0.338396 black VPS29 -0.054617 0.237319 black 2-Sep -0.080195 -0.009829 black TPI1 0.353888 -0.338779 black NCSTN -0.151955 0.237197 black NACA -0.211916 -0.010047 black PITRM1 0.070389 -0.339108 black SMC3 -0.171141 0.237074 black GCN1L1 -0.161395 -0.010062 black ZMYND8 -0.165418 -0.340496 black ATP6V1F -0.409566 0.236707 black HRSP12 -0.041699 -0.010076 black SLC25A4 0.656359 -0.340605 black AIP -0.437975 0.236584 black SMARCA4 -0.060653 -0.010251 black NMT2 0.052833 -0.341994 black COMMD3 -0.095959 0.235727 black RPLP2 -0.036481 -0.010498 black EPDR1 0.218099 -0.342891 black DDX19B -0.217290 0.235482 black CTTN -0.057707 -0.011123 black WDR44 0.343124 -0.343604 black IPO4 -0.327620 0.235482 black GALNT2 -0.180659 -0.011210 black LAMP2 0.494672 -0.345913 black TUBGCP3 0.004178 0.234992 black CYC1 -0.018133 -0.011254 black CTSA 0.127897 -0.346335 black CUL3 -0.117490 0.234869 black RMDN1 0.152248 -0.011690 black DDAH2 0.186247 -0.346610 black ADSL -0.074289 0.234501 black PRPSAP2 -0.153880 -0.011704 black STMN1 0.052972 -0.347325 black TJP2 -0.555460 0.234501 black AP2A1 0.137635 -0.012315 black YIF1B 0.630592 -0.347950 black TMA7 -0.450853 0.234011 black IGF2BP3 0.019488 -0.012374 black CHCHD3 -0.224435 -0.349787 black WDR3 0.015926 0.233888 black EIF4E -0.001877 -0.012374 black IFI16 0.241108 -0.350008 black MYO1C -0.161379 0.233029 black PHB 0.075738 -0.012490 black FUBP1 0.118094 -0.350118 black STX8 -0.180528 0.233029 black RSL1D1 -0.016804 -0.012563 black ATP12A -0.181100 -0.351332 black DCAKD -0.573016 0.233029 black DDAH1 -0.068695 -0.012606 black MTX1 -0.282562 -0.351958 black AGK 0.187768 0.232906 black TARS -0.031238 -0.012781 black INPPL1 0.029418 -0.352400 black GNPAT -0.392251 0.232538 black RBBP7 -0.678811 -0.012810 black IGF2BP1 0.464459 -0.353026 black GRSF1 -0.814007 0.232538 black IPO9 -0.175957 -0.012868 black CSTF2 -0.674591 -0.355258 black NME2P1 -0.037547 0.232292 black AP1M1 0.016496 -0.013043 black PML 0.698041 -0.355645 black POLR2B -0.136395 0.232047 black EPRS 0.072586 -0.013349 black ATP6V1H 0.073272 -0.355959 black NCAPG -0.223374 0.232047 black EWSR1 -0.145446 -0.013742 black HDGFRP2 0.082703 -0.357308 black EIF3L -0.091633 0.231555 black NDUFB10 0.004753 -0.014004 black PDIA3 -0.203103 -0.357382 black MRPL11 -0.206362 0.231432 black TM9SF2 -0.103596 -0.014893 black FTL 0.305679 -0.357973 black PFDN5 -0.016337 0.231310 black YWHAZ 0.053389 -0.015112 black KATNAL2 -0.011792 -0.358472 black NT5DC2 -0.174687 0.231064 black CARS 0.069427 -0.015360 black PTRH2 -0.110371 -0.358491 black RNF40 0.166587 0.230449 black SPAG9 -0.221791 -0.015505 black CYB5R3 -0.006855 -0.358639 black LGALS3 -0.003467 0.230203 black ARHGAP35 -0.284247 -0.015520 black DYNLT3 0.586020 -0.359786 black ELAVL1 -0.345473 0.229711 black NEDD8 -0.019799 -0.015841 black SLC12A4 -0.047488 -0.361250 black CYFIP2 0.401521 0.229342 black ARL8A 0.272023 -0.015928 black DHX9 0.185359 -0.361639 black KIAA1033 -0.076828 0.229342 black NSFL1C -0.032728 -0.016089 black HMGB1 0.101112 -0.361843 black RAB10 -0.219186 0.229342 black CRK 0.216610 -0.016206 black PCYOX1 0.183963 -0.362696 black KLC1 -0.174784 0.228973 black CARS2 -0.215982 -0.016366 black MAN2B1 0.486869 -0.363828 black OCRL -0.171255 0.228726 black RELA -0.494536 -0.016366 black AK3 0.350271 -0.364794 black ASNA1 -0.404859 0.228357 black KTN1 0.098285 -0.016410 black CSNK2B 0.024745 -0.364886 black MRPS15 -0.418292 0.228111 black UQCRQ 0.192825 -0.017738 black HEXA 0.311736 -0.365202 black MRPS12 -0.260895 0.227987 black PTPN23 -0.471309 -0.018016 black MTCH2 -0.137124 -0.365741 black EIF2S2 -0.231430 0.227495 black WDFY1 0.521654 -0.018352 black LEPREL2 -0.035520 -0.365927 black NCAPH -0.065416 0.227002 black PLAA 0.074916 -0.018673 black IQGAP3 0.152119 -0.367173 black NDUFAF4 -0.259979 0.226262 black HRAS 0.230572 -0.018863 black RETSAT -0.346096 -0.367490 black WASF2 0.055890 0.226139 black DTD1 -0.031415 -0.019229 black WIBG -0.278741 -0.369539 black SPC24 -0.198477 0.225768 black CLPTM1 -0.516904 -0.019375 black HNRNPK 0.234011 -0.369688 black SNAPIN -0.261448 0.225398 black AP1S3 0.185867 -0.019507 black HMGN1 -0.220900 -0.371721 black GID8 -0.319424 0.225152 black CCNL1 0.132379 -0.019653 black PGAM1 0.312665 -0.372281 black UBE4A 0.013069 0.225028 black VPS33B -0.378719 -0.019814 black TMEM230 -0.032669 -0.373066 black CNOT1 -0.215563 0.224905 black HYOU1 -0.051908 -0.020106 black WDR5 0.125916 -0.373421 black FKBP15 0.022048 0.224781 black SDCBP -0.443517 -0.020165 black RPS6KA3 -0.418041 -0.373757 black ASL -0.177164 0.224658 black CUL5 0.219835 -0.020458 black GPRC5A -0.253326 -0.374730 black RAB12 -0.186393 0.224658 black NUMA1 -0.056086 -0.020531 black RAB2B -0.032285 -0.375141 black HSP90AB1 -0.300626 0.224658 black ALDH1A3 -0.514965 -0.020779 black PTPMT1 -0.207478 -0.375497 black NAA25 -0.045789 0.224040 black HIST1H1E 0.101919 -0.020955 black LRRC47 -0.041937 -0.376807 black ANKRD28 -0.292811 0.223546 black RRAS 0.018064 -0.021277 black AMPD2 0.034497 -0.378494 black GTPBP10 -0.789137 0.223546 black PAFAH1B1 0.073272 -0.021394 black C19orf10 0.151469 -0.379020 black YPEL5 -0.121110 0.223423 black SLC25A11 0.152378 -0.021585 black ACO2 0.186247 -0.379827 black SNX3 -0.076935 0.223175 black TES -0.246105 -0.021922 black RPL29 -0.445716 -0.379827 black HDHD1 -0.300413 0.222928 black DPP3 -0.084839 -0.022332 black SMAD3 -0.501300 -0.380934 black ITGA3 -0.299543 0.222805 black EIF5A -0.028396 -0.023182 black PRPF3 -0.146004 -0.381686 black ESYT1 -0.313459 0.222557 black FAM129B 0.022332 -0.023490 black POLR2M -0.409949 -0.383172 black PPID -0.494495 0.222186 black SNX5 0.115965 -0.023534 black GAR1 0.393306 -0.385073 black RAB3GAP2 -0.283492 0.221939 black PRMT5 -0.195649 -0.023680 black MBNL1 -0.227996 -0.385563 black KPNA3 -0.244189 0.221568 black CAMK1 0.040963 -0.023695 black PPOX 0.468427 -0.386638 black RTN1 0.017637 0.221444 black EPHB4 -0.131803 -0.024223 black GOT1 0.255440 -0.387110 black PCNA -0.194559 0.221073 black S100A6 -0.030059 -0.024238 black HEXB 0.299127 -0.390793 black C10orf76 0.076422 0.220949 black YWHAQ 0.038436 -0.024517 black IMMT 0.076012 -0.391323 black UTRN -0.468112 0.220454 black ARFGEF1 0.207518 -0.024839 black PLOD2 0.510759 -0.391569 black STAT3 -0.067500 0.220206 black PPT1 0.310922 -0.025030 black AKR1A1 0.151079 -0.392629 black COMMD4 -0.024062 0.220082 black NSUN2 0.047678 -0.025456 black EMD 0.174023 -0.393084 black RAD21 -0.100561 0.220082 black PSMA5 0.088142 -0.025853 black HADHB 0.325847 -0.393274 black RPAP1 -0.246755 0.220082 black PSME2 0.169412 -0.027425 black PHPT1 0.305329 -0.394051 black HSPA6 -0.246806 0.220082 black SPRYD4 0.029559 -0.027513 black P4HA2 0.167358 -0.394335 black ARHGDIA -0.074882 0.219463 black XRN2 0.016924 -0.027822 black SEC23A 0.227125 -0.395473 black LMO7 -0.236673 0.219215 black RHEB 0.013927 -0.027925 black DLST -0.008276 -0.398094 black BCR -0.178567 0.219091 black REXO2 -0.021614 -0.028072 black PRDX4 0.320427 -0.398494 black NLN -0.212885 0.218471 black MTPN -0.097548 -0.028131 black Nbla03646 -0.343128 -0.399464 black GCLM -0.203186 0.217851 black OGDH 0.153416 -0.028293 black ACOT9 0.370722 -0.400358 black SARS -0.318110 0.217851 black SEC16A -0.094695 -0.028440 black SORD -0.360527 -0.400625 black RAP1GDS1 -0.218699 0.217603 black XRCC1 -0.196145 -0.028440 black NCEH1 0.130404 -0.401940 black DNPEP -0.104697 0.216734 black PSMB5 0.157173 -0.028602 black SOD1 0.422018 -0.401959 black SUB1 -0.268747 0.216362 black SPCS2 0.074642 -0.028940 black CHCHD2 -1.387412 -0.402016 black MRP63 -0.045774 0.215741 black CPT1A 0.063503 -0.029161 black ENO2 0.415434 -0.402054 black LIG1 -0.317750 0.215368 black PDHB 0.065572 -0.029426 black SERPINB2 0.235972 -0.402435 black MGEA5 -0.212685 0.214747 black ECH1 0.122474 -0.029559 black GPX1 0.162983 -0.403809 black WDR61 -0.346995 0.214498 black PFN2 0.274888 -0.029986 black SDR39U1 0.323312 -0.405108 black WAPAL -0.569501 0.213876 black RPS20 -0.101443 -0.030104 black RIN1 1.738508 -0.405623 black CALD1 -0.177670 0.213130 black Em:AP000351.3 0.286053 -0.030472 black MMP14 -0.207578 -0.406312 black PEX14 -0.254634 0.213005 black SEC13 0.058524 -0.030781 black EPB41L1 0.297368 -0.406388 black EIF4A2 -0.045149 0.212632 black PFN2 0.177471 -0.030988 black RPL26L1 0.191310 -0.406388 black PARP1 -0.282176 0.212632 black RARS 0.088684 -0.031076 black HIST1H1C -0.649276 -0.408723 black PPP1R7 -0.244651 0.211635 black MRPS33 -0.073454 -0.031091 black GNG5 0.051024 -0.411311 black KPNA6 -0.201327 0.211386 black EMC2 0.053389 -0.031194 black YLPM1 -0.164011 -0.411445 black HSD17B12 -0.210363 0.211137 black CSE1L 0.098824 -0.031858 black TNFRSF10B 0.201257 -0.413423 black SREK1 -0.276415 0.211137 black EIF4H -0.109857 -0.032758 black NAGLU 0.323082 -0.414211 black FMNL3 -0.073135 0.210638 black LARP7 0.016496 -0.032817 black SLC12A9 0.362217 -0.414441 black S100A16 -0.163688 0.210264 black NDUFA5 -0.146883 -0.032935 black COX6A1 -0.262538 -0.415095 black SRP54 -0.163801 0.210139 black UBXN6 -0.223104 -0.033510 black ATP6V0A1 1.042294 -0.415711 black COMMD9 -0.312581 0.209641 black MTMR9 0.578890 -0.034367 black SCAMP3 -0.069225 -0.416481 black EEF1A1 -0.306020 0.209516 black HSPA13 -0.319550 -0.034618 black JUP 0.459537 -0.417039 black GOLGA4 -0.470549 0.209266 black CDK5 0.085153 -0.034840 black TIGAR 0.216113 -0.417097 black FAM98A -0.205963 0.208892 black MRPS14 -0.122350 -0.035032 black TMEM256 0.158337 -0.417232 black MRPL33 0.130008 0.208018 black ANXA7 0.140255 -0.035047 black SLC25A12 0.057831 -0.418504 black LPP -0.589985 0.208018 black EMC4 -0.268243 -0.035210 black HM13 0.003458 -0.418504 black RPL38 -0.130460 0.207893 black MCU 0.058247 -0.035712 black TMPO 0.268674 -0.419777 black VASP -0.158768 0.207643 black CDC42BPB 0.247198 -0.035786 black SCAMP1 0.251810 -0.419797 black CDO1 -0.316061 0.207518 black ERH 0.011782 -0.035801 black NDUFB11 -0.679642 -0.420048 black HDAC1 -1.210663 0.207518 black DPYSL3 -0.072756 -0.036023 black VAMP4 0.886121 -0.421187 black MED4 -0.236333 0.207393 black NFKB2 -0.323173 -0.036112 black DERL1 0.313942 -0.422366 black ACYP1 -0.140826 0.207268 black EIF5 0.024603 -0.036260 black NHP2L1 0.260748 -0.424881 black ZMPSTE24 -0.240759 0.207018 black ALCAM -0.241492 -0.036674 black HADHA 0.398350 -0.426529 black NT5DC3 -0.103565 0.206768 black RPTOR -0.285407 -0.037014 black POTEE -0.592245 -0.426858 black GALNT1 -0.534391 0.206768 black AP2M1 0.031113 -0.037236 black CPSF1 -0.265414 -0.431442 black CCT2 -0.124707 0.205893 black HSPA5 0.049073 -0.037962 black TOMM7 -0.206362 -0.432104 black TMX3 -0.361861 0.205393 black LPCAT2 -0.179188 -0.038258 black CORO1C 0.680954 -0.434988 black CHMP6 0.044324 0.205142 black PPA1 -0.229247 -0.038940 black AK1 0.286881 -0.436725 black MYO5A 0.149910 0.205017 black CLTA 0.052277 -0.038984 black MVP 0.393087 -0.440890 black MRPL43 -0.077817 0.204892 black TSTA3 -0.004638 -0.038984 black FUS -0.070391 -0.442497 black ADAR -0.313477 0.203389 black HSPA9 -0.044776 -0.039073 black SDHC 0.045443 -0.443046 black EIF3A -0.294880 0.202512 black CALR -0.025735 -0.039221 black ERO1L 0.491802 -0.445146 black GSTK1 -0.579189 0.202136 black SNRPD3 0.118227 -0.039622 black KHDRBS1 0.425030 -0.445185 black TMEM109 -0.060698 0.201759 black SCFD1 -0.141001 -0.039740 black SOD2 0.566377 -0.447446 black COPS7B -0.076007 0.201634 black C20orf27 -0.284300 -0.039755 black NDUFS7 -0.143548 -0.449513 black CCDC6 -0.015287 0.201132 black DHRS7B -0.050592 -0.040067 black SETD3 -0.785776 -0.450834 black IDE -0.510909 0.201132 black PYCRL -0.438601 -0.040319 black OSBPL3 -0.235739 -0.451563 black GIPC1 -0.433156 0.200630 black BTAF1 -0.015608 -0.040467 black NUCB2 0.322505 -0.452254 black MRPL14 -0.147761 0.200253 black DECR2 -0.536691 -0.040541 black DPP7 0.754888 -0.452274 black UGGT2 -0.019916 0.200128 black CSTF3 -0.109717 -0.040719 black PPIH 0.127633 -0.453202 black PITPNB -0.218145 0.200128 black PLS1 -0.560090 -0.040838 black ILF2 0.201383 -0.453636 black STK39 -0.057091 0.199625 black RTCA 0.185867 -0.041180 black C16orf13 0.062260 -0.454565 black PLCB3 -0.455752 0.198997 black CMPK1 0.001586 -0.041625 black PSPC1 0.173639 -0.456385 black ACACA -0.507193 0.198494 black SNRPB2 0.165558 -0.042041 black LMNA 0.192194 -0.457950 black MAN1B1 0.120219 0.197111 black PDCD6 0.172488 -0.042293 black DNAJB11 -0.008827 -0.458168 black BIN1 -0.124047 0.196733 black CHD4 -0.139268 -0.042888 black SELH -0.039088 -0.461740 black RPL35A -0.040645 0.196607 black DTYMK 0.192952 -0.043274 black ALDH7A1 0.449957 -0.461978 black FAS 0.100036 0.195977 black AASS 0.562181 -0.043438 black RBM15 0.252537 -0.463887 black HMBS -0.350210 0.195977 black SRP68 0.043345 -0.043765 black TMX2 0.191057 -0.465759 black HSPB11 -0.073105 0.195852 black SRI 0.146395 -0.043914 black HIST1H1B 0.105812 -0.467134 black YARS -0.133179 0.195096 black LANCL1 -0.099448 -0.044018 black GLRX 0.356820 -0.468152 black TBC1D9B 0.069152 0.194970 black NDUFS6 0.023610 -0.044196 black ACTBL2 -0.474673 -0.468651 black PDCD5 -0.132198 0.194970 black SCFD2 -0.078122 -0.044583 black ASAH1 0.181548 -0.468771 black SRPR -0.177980 0.194717 black DPY19L1 0.276080 -0.045074 black PDE5A 0.167101 -0.468791 black DCTD 0.130008 0.194591 black MYADM 0.061016 -0.045253 black LMNA 0.388465 -0.470409 black PDAP1 -0.316061 0.194339 black AP1G1 0.120883 -0.045268 black CRIP2 0.019488 -0.470449 black PLCG2 0.022474 0.193961 black TIMM9 0.162339 -0.045565 black APAF1 -0.274163 -0.474653 black CKAP5 -0.236486 0.193583 black RPLP1 -0.054168 -0.045714 black MFSD10 0.245861 -0.478246 black PFAS -0.328852 0.193583 black SNX12 -0.178437 -0.045819 black THOC2 0.350045 -0.482372 black VBP1 -0.414730 0.193456 black PSMB7 0.142348 -0.045982 black MYEF2 0.744161 -0.482896 black PPP1CA -0.253532 0.193078 black ACAT2 -0.137934 -0.045997 black SMPD1 0.265317 -0.484631 black EIF2B1 0.006908 0.192952 black RPL4 -0.020428 -0.046087 black PGK1 0.622462 -0.486570 black SMTN -0.743376 0.192573 black SERBP1 -0.077163 -0.046414 black BCL2L13 -0.490335 -0.486570 black ABR -0.058097 0.192194 black ATP6V1E1 0.044324 -0.046847 black SPCS1 -0.402016 -0.488714 black PRRC1 -0.156016 0.192194 black TOM1 -0.116895 -0.047040 black HNRNPAB 0.332393 -0.488836 black TRIM23 -0.076539 0.191942 black LEPRE1 0.016782 -0.047368 black TMPO 0.115033 -0.490841 black MTHFD1L -0.396745 0.190931 black RPL13 -0.121330 -0.047577 black KCNN4 -0.095265 -0.496020 black BAG5 -0.234805 0.190425 black PSMA2 0.028286 -0.047741 black NT5C -0.220177 -0.496020 black MNF1 -0.137267 0.190299 black PGM3 0.047957 -0.048338 black ILF3 0.501210 -0.496896 black COPS3 -0.182328 0.190046 black CPSF6 0.112100 -0.048576 black KDSR 0.223917 -0.497629 black ACTR10 0.062812 0.189919 black THOP1 -0.023035 -0.048770 black MGST1 -0.004740 -0.498831 black VTA1 0.067914 0.189793 black C1orf123 -0.109701 -0.049278 black HK1 0.367147 -0.500116 black RPS28 -0.129071 0.189540 black VAPA -0.111025 -0.049293 black GOLT1B 0.092072 -0.500871 black HSP90AB4P -0.088147 0.189034 black KIAA0196 -0.115691 -0.049472 black ZNF622 0.044884 -0.502321 black RAB23 -0.398570 0.188907 black CAPN2 -0.018235 -0.049502 black COPZ2 -0.206246 -0.502546 black NAP1L1 -0.304808 0.188401 black NOL9 0.033652 -0.050084 black ORMDL2 0.230941 -0.506189 black RBM17 -0.096653 0.188021 black PSMD9 0.234501 -0.050308 black ARL6IP5 0.134221 -0.506988 black PHGDH -0.163446 0.187894 black TBCD 0.031677 -0.050502 black CPD 0.085561 -0.507890 black PTPN14 -0.812764 0.187134 black GARS -0.001270 -0.050547 black HMGA1 -0.245557 -0.509348 black TROVE2 -0.061225 0.186881 black AP3D1 0.117030 -0.050846 black MT-ATP6 0.552869 -0.509389 black L3HYPDH -0.047890 0.186247 black FXR1 0.004178 -0.051429 black FAM162A 0.565890 -0.511876 black RNF123 -0.067803 0.186247 black TWF2 -0.246960 -0.051459 black FAM213A 0.272143 -0.512102 black UBL4A -0.441693 0.186247 black EXOSC4 -0.167572 -0.051818 black SRSF7 0.329066 -0.512493 black RNF213 -0.394923 0.185867 black RACGAP1 0.114767 -0.051952 black THRAP3 0.136191 -0.513625 black GNA13 -0.571858 0.185486 black EIF6 -0.171856 -0.052012 black NME3 -0.428935 -0.514099 black GMPS -0.248176 0.185359 black TXNDC17 0.113834 -0.052117 black AAMDC 0.518636 -0.518205 black CNOT7 -0.558750 0.185359 black FH -0.029264 -0.052416 black RBM8A 0.513996 -0.519921 black INF2 -0.374991 0.184344 black ACADM 0.062674 -0.052611 black SF3B2 -0.105799 -0.523919 black CHCHD1 -0.230973 0.183963 black RAB1A 0.137504 -0.052985 black GBA 0.327917 -0.526265 black ECD -0.646406 0.183582 black MXRA7 -0.372038 -0.053000 black UBR5 0.025879 -0.526888 black PPFIA1 -0.152276 0.183455 black TMED7 0.357383 -0.053254 black RECQL 0.541217 -0.527387 black GLG1 -0.302885 0.183328 black KIAA1279 0.067226 -0.053404 black ITGAV 0.158725 -0.528615 black RPS27A -0.260100 0.183201 black PKN1 -0.237830 -0.053688 black ALDH4A1 0.371392 -0.532804 black NIP7 0.042924 0.182946 black ACYP2 0.085561 -0.053748 black GALK1 -0.140539 -0.533201 black RPL24 -0.125321 0.182946 black NCBP1 0.358509 -0.053853 black RBM10 0.273456 -0.534935 black TRMT1 -0.261120 0.182438 black G6PD -0.018659 -0.053868 black SCYL1 0.169925 -0.536545 black YWHAB -0.207778 0.182311 black PMM2 -0.060382 -0.053883 black POFUT2 0.341872 -0.539205 black CAPZB 0.000577 0.182057 black NDUFAB1 -0.150433 -0.053973 black SRSF2 0.386038 -0.540338 black TBCA -0.183343 0.182057 black MRPL28 -0.218313 -0.054063 black SAMM50 0.146525 -0.541345 black EHD4 0.113167 0.181802 black USP14 0.062812 -0.054093 black SF3B1 0.177599 -0.543026 black ATP6V1C1 -0.069452 0.181548 black FAH -0.153864 -0.054692 black PRDX3 0.457227 -0.549198 black RPS24 -0.174866 0.181548 black DPP9 0.329985 -0.054707 black HMGA2 -0.406771 -0.553553 black ALG3 -0.029573 0.181293 black CBR1 0.138159 -0.054782 black CD68 -0.155020 -0.554189 black RPL36A -0.000260 0.181166 black CPS1 0.038717 -0.055157 black PLIN3 0.438293 -0.555948 black RAB3GAP1 -0.425482 0.181166 black TSFM -0.179596 -0.055187 black SPARC 0.012354 -0.558942 black PLRG1 -0.352455 0.180912 black U2AF1 0.086512 -0.055366 black CRAT 0.204015 -0.563540 black NFU1 -0.326208 0.180657 black UBXN7 -0.438229 -0.055906 black PTTG1IP -0.493053 -0.567939 black BRAT1 -0.517399 0.180403 black GANAB -0.003886 -0.056116 black PTMS -0.003134 -0.568195 black MEMO1 -0.466736 0.180275 black PA2G4 -0.050727 -0.056671 black DDX39B 0.653427 -0.574927 black RPP30 -0.441008 0.180148 black CHP1 -0.180119 -0.056671 black TPP1 0.476952 -0.577509 black UBR4 -0.015870 0.179893 black CLIC4 -0.133337 -0.056926 black GLB1 0.477366 -0.579016 black PPP2CA -0.134287 0.179893 black SUMF2 -0.292228 -0.057046 black ACADVL 0.436268 -0.579081 black MAPRE1 -0.339382 0.179893 black ATP5I 0.193583 -0.057542 black ITGA2 0.323658 -0.580461 black RRP12 -0.311381 0.179766 black ALDH9A1 0.156008 -0.057722 black ITGA2 0.290542 -0.580569 black CCT4 -0.110199 0.179511 black TSN 0.052694 -0.057947 black TMBIM6 0.349592 -0.580634 black ZNF207 -0.751368 0.178237 black MYO18A 0.060186 -0.058398 black MT-ATP8 0.106616 -0.582361 black HOOK3 0.121413 0.176067 black ARPC1B 0.224040 -0.058638 black OSTC 0.003746 -0.582901 black ILVBL 0.008630 0.175684 black USMG5 -0.045878 -0.058668 black LMNB1 0.478817 -0.585020 black CDC37 -0.234058 0.175557 black COX7B -0.082836 -0.059119 black SRSF9 0.069977 -0.585929 black UMPS -0.206096 0.175429 black PSMD1 0.012497 -0.059224 black TOMM22 -1.058112 -0.586579 black TPX2 -0.534663 0.175429 black SET -0.106001 -0.059766 black DDX17 0.359521 -0.587013 black UFC1 -0.268330 0.175301 black PTRHD1 -0.081354 -0.059826 black IRF2BPL 0.115566 -0.589116 black GSK3B -0.365964 0.175301 black PREP 0.138028 -0.060292 black UBE2S 0.129481 -0.590962 black ABCE1 -0.274512 0.175173 black VPS35 0.191815 -0.060307 black HNRPDL 0.098285 -0.591962 black NTMT1 0.091260 0.174534 black PABPC4 0.002595 -0.060307 black UBTD1 -0.925193 -0.594596 black BLOC1S2 -0.091402 0.174534 black NECAP2 0.245131 -0.060322 black PC 0.129481 -0.595576 black SUPT4H1 -0.114332 0.174023 black ALG1 -0.466516 -0.060473 black ARSB 0.253142 -0.597169 black DDRGK1 -0.292334 0.173127 black EHD3 -0.074441 -0.060533 black MAGOHB 0.669117 -0.598064 red PSMD14 -0.174833 0.172999 black CASP6 0.175301 -0.060999 black HNRNPA2B1 0.497229 -0.599375 black MUT 0.160791 0.172744 black SH3GLB1 -0.056011 -0.061059 black KPNA2 0.368377 -0.604016 black DDX24 -0.068196 0.172616 black COA3 -0.130523 -0.061165 black HNRNPR 0.336855 -0.606342 black ARF1 -0.222835 0.172359 black NOP2 -0.012388 -0.061360 black OVCA2 -0.784160 -0.610543 black NUP98 -0.451623 0.172103 black PSMC2 -0.000606 -0.061541 black KIF23 0.539233 -0.613895 black SACM1L 0.009347 0.171079 black PHB2 0.115433 -0.061586 black HIST2H2BE 1.009491 -0.621932 red EIF3B -0.070360 0.171079 black ARPC5L 0.428357 -0.062023 black OSBP 0.085697 -0.622399 black NUDT16 -0.210696 0.169925 black DDX23 0.190172 -0.062083 black P4HA1 0.660107 -0.626736 red AASDHPPT -0.301728 0.169925 black MANF -0.070027 -0.062098 black SIAE -0.018074 -0.630975 black POR -0.176577 0.169412 black RPS26 -0.216636 -0.062249 black TOP1 0.702214 -0.631064 red GSS -0.003944 0.168899 black C15orf52 -0.140810 -0.062656 black G6PC3 -0.213872 -0.640164 black TRIM28 -0.096638 0.168770 black SCCPDH 0.108491 -0.062957 black RAB8B 0.350497 -0.644826 black CAV1 -0.068529 0.168385 black HNRNPU 0.026446 -0.063093 black MGST3 0.293253 -0.651292 black SFN -0.075794 0.168385 black HINT1 0.195474 -0.063213 black ACTN1 0.910426 -0.651722 red GNAS -0.139140 0.168385 black MTAP -0.102667 -0.063334 black C12orf23 0.131589 -0.657809 black CAPZA2 -0.211214 0.168385 black HNRNPLL -0.077559 -0.063485 black PI4K2A 0.475500 -0.658379 black TSG101 -0.122805 0.168129 black UCHL1 0.185486 -0.063922 black FUCA2 0.162983 -0.661981 black PPP6R3 -0.141382 0.168129 black PGM2 0.136848 -0.063967 black HNRNPA3 0.495388 -0.662118 black SLC3A2 -0.200664 0.168000 black ALDH16A1 -0.064027 -0.063967 black SDC4 -0.227270 -0.663739 black PITHD1 0.196859 0.167743 black SSR4 0.134089 -0.064208 black HEATR1 0.614286 -0.668157 red TRADD -0.242704 0.167615 black MAGT1 0.276318 -0.064254 black SQSTM1 -0.435475 -0.672865 black PACSIN2 0.141825 0.167486 black MAPK3 -0.363884 -0.064510 black ITPRIP 0.647176 -0.674406 red STIP1 -0.107368 0.167229 black EPS15L1 0.082294 -0.064555 black GM2A 0.285225 -0.685965 black CCT7 -0.047040 0.165687 black TNPO3 -0.001097 -0.064797 black BLM 0.434775 -0.692408 black ARF6 -0.101939 0.165687 black GPI 0.239031 -0.065234 black DCTN5 0.055890 -0.702515 black PANK4 0.078337 0.165558 black DCPS -0.139617 -0.065506 black SRSF3 0.183328 -0.719281 black TMEM165 -0.176528 0.165558 black SLC4A1AP 0.840524 -0.065536 black LXN 0.676448 -0.727714 red GNB2 -0.147522 0.165172 black PSMC4 0.074642 -0.065838 black FBL 0.495593 -0.750979 black SNX24 -0.043646 0.165044 black RAB5B -0.163026 -0.066261 black CHMP5 -0.249702 -0.756989 black CCT6A -0.087166 0.164786 black EMC3 -0.157335 -0.066322 black FTH1 0.767655 -0.759820 red NDUFA6 -0.219052 0.164271 black U2SURP -0.052341 -0.066578 black H2AFV 0.646070 -0.760871 red EXOSC8 -0.639535 0.163628 black AHSA1 -0.228588 -0.066609 black TOMM6 -0.805409 -0.770314 black LAMB1 -0.433682 0.162726 black PMPCA -0.047488 -0.066699 black GTPBP4 0.461738 -0.770757 black CSK -0.185048 0.162339 black BANF1 0.627513 -0.066926 black CASK 0.746055 -0.773738 red RPA2 -0.264530 0.161823 black CFL2 0.263756 -0.067440 black ITM2B 1.050815 -0.777913 red DPM3 -0.211364 0.161694 black METAP1 -0.108176 -0.067440 black NOP58 1.149650 -0.804400 red PIGT -0.000491 0.161436 black NUP214 -0.807276 -0.067470 black HMGA1 -0.190404 -0.804652 black VKORC1L1 -0.120828 0.160662 black LEPREL4 0.068464 -0.067561 black VDAC1 0.088684 -0.813956 black USP15 -0.300448 0.160662 black PTCD3 0.022048 -0.067833 black CLGN -0.051040 -0.814717 black EIF4G3 -0.474773 0.160533 black HSP90B1 0.094506 -0.067969 black PDXP -0.138997 -0.821687 black MTHFS -0.323047 0.160017 black TMED4 -0.044881 -0.067969 black FUCA1 0.732313 -0.829229 red EPB41L2 -0.228098 0.159887 black SDHD 0.269991 -0.068090 black PLAT 0.459641 -0.840009 black HSPA2 -0.238476 0.159887 black PPIL3 0.055473 -0.068151 black SCRN3 0.570949 -0.847804 black ARL2 0.044604 0.159758 black COX4I1 -0.135713 -0.068256 black TRA2B 0.436908 -0.849206 black HSP90AA1 -0.179155 0.159758 black ACAT1 0.106482 -0.068347 black ZC3H4 -0.149825 -0.858907 black DCUN1D1 -0.071815 0.159500 black DDX1 0.019346 -0.068559 black VIM 0.682573 -0.862811 red ACTR2 0.085833 0.158983 black ORMDL1 -0.384565 -0.068589 black FDXR 0.482642 -0.864097 black CCT8 -0.101846 0.158466 black PSMC1 0.082566 -0.068725 black IGFBP7 0.121944 -0.865726 black EEF1D -0.234584 0.158078 black DCTN1 0.119821 -0.068741 black GAA 0.801655 -0.881507 red DRG1 -0.451386 0.157691 black ACOT13 0.214622 -0.068771 black CACNA2D1 0.723253 -0.891884 red ATP2A3 0.048097 0.157432 black SMS -0.021204 -0.068801 black ACTN4 0.935309 -0.900395 red UROS -0.070209 0.157432 black MESDC2 -0.397752 -0.068846 black VDAC2 0.393855 -0.907305 black CDC5L -0.222263 0.156655 black DNPH1 -0.325340 -0.069104 black GSN 0.753262 -0.917107 red SAR1A -0.115535 0.156526 black ARFIP1 0.076696 -0.069164 black NOP56 0.646716 -0.918415 red UBE2O -0.394885 0.156526 black SAR1B -0.375834 -0.069467 black HIST1H2BL 0.647361 -0.960384 red C16orf62 -0.029662 0.156008 black UBE2I -0.351976 -0.069967 black DHRS3 0.276437 -0.994528 black ITGB1 -0.202074 0.155749 black GRPEL1 0.057693 -0.070194 black VDAC3 -0.071421 -0.997348 black PRKCA -0.415268 0.155620 black NIPSNAP3A 0.485633 -0.070224 black HIST2H2AB 0.829281 -0.998385 red CRTAP -0.173108 0.155231 black TLDC1 0.083111 -0.070239 black PNN -0.063017 -1.018381 black MMGT1 -0.129655 0.155102 black DAK -0.025030 -0.071179 black DNAJC4 -0.204316 -1.062113 black PAIP1 -0.174296 0.155102 black NDUFS8 -0.030118 -0.071376 black TTC17 0.088413 -1.067364 black EIF2AK2 -0.227253 0.154713 black SEC61B -0.230076 -0.071527 black TOMM40 -0.712339 -1.087335 black PSMB8 0.104337 0.154454 black NDUFB5 -0.135713 -0.071588 black PBXIP1 0.862352 -1.121769 red LPCAT1 -0.164335 0.154454 black GOSR2 0.004178 -0.071800 black DBN1 0.477885 -1.131787 black DNAH1 -0.450479 0.154324 black TRIP12 -0.378457 -0.071861 black S100A10 0.387032 -1.245882 black ABCD3 -0.265379 0.153805 black ACAD9 0.053945 -0.072028 black CRABP2 0.512379 -1.270417 black SRPRB -0.216636 0.153416 black ANXA4 0.222434 -0.072104 black HMGN3 -0.457593 -1.306466 black PFDN2 -0.291857 0.153157 black PSMD10 0.108625 -0.072240 black HIST2H2AC 0.352646 -1.357973 black SERPINH1 -0.006434 0.152897 black LAS1L 0.144699 -0.072543 black HNRNPH3 0.649018 -1.381498 red NSDHL -0.254857 0.152768 black CLASP1 -0.284704 -0.072604 black UBE2V2 -0.516739 -1.471529 black PDXK -0.288399 0.152638 black SYNCRIP 0.060739 -0.072680 black NDRG1 1.796058 -1.543888 red MICU1 0.350045 0.152508 black ACAA1 -0.450696 -0.072817 black TXNIP 1.555424 -1.558092 red DAB2 -0.172067 0.152248 black UBXN4 -0.606123 -0.072877 black RNGTT -1.440459 -1.560856 black FAM50A -0.520293 0.152248 black PSIP1 -0.320216 -0.073059 black IL8 1.273635 -1.699841 red NUDCD1 0.095992 0.152119 black ERP44 0.401630 -0.073530 black ZNF638 -0.092863 -1.714870 black OTUB1 -0.067364 0.152119 black NPTN -0.083799 -0.073621 black SERPINE2 1.062536 -1.716623 red DHX29 -0.095836 0.151599 black SLC25A10 -0.086967 -0.073894 black SAFB 1.191057 -1.871073 red SHCBP1 -0.129781 0.151469 black NMT1 0.164786 -0.073925 black KIF20A 1.200002 -1.955900 red RPL26 -0.219287 0.151469 black MPDU1 -0.079326 -0.074077 black DNAH9 -0.276363 -1.978066 black RPL36AL 0.111432 0.150690 black DNAJA2 -0.167183 -0.074198 black H3F3B 2.299479 -2.214575 red NHLRC2 0.025171 0.150560 black PPIB 0.214498 -0.074623 black HNRNPC 2.280006 -2.227760 red RPS11 -0.066518 0.150430 black DDOST 0.045862 -0.074623 black MROH1 -0.415134 -2.317175 black MYH9 0.008917 0.149650 black TMEM126A -0.139474 -0.074775 black DMD 0.638491 -2.321712 red RPL12 -0.100701 0.149650 black NOP10 0.173639 -0.075049 black AGRN -2.593354 -2.424998 black RDX -0.067183 0.149520 black POLR1C -0.653650 -0.075125 black HIST1H4A 1.624335 -2.548691 red MRPL32 -0.314804 0.149389 black THY1 0.205643 -0.075307 black RBMX 1.894410 -2.634688 red LIPA -0.705735 0.149389 black FUBP3 -0.125966 -0.075429 black HIST1H3A 2.183614 -2.803393 red LAP3 -0.234992 0.149129 black EPN3 0.019631 -0.076387 black HMOX1 1.958137 -2.900557 red KIF14 -4.187050 -3.016249 black IL1B 2.444216 -3.191920 red Supplementary Table S2. List of antibodies.

Antigen Antibody name Catalogue Company

number

β-Actin β-Actin (C4) Mouse mAb sc-47778 Santa Cruz Biotechnology

P62 SQSTM1/P62 (D3) Mouse mAb sc-28359 Santa Cruz Biotechnology

TP53 p53 (DO-1) Mouse mAb sc-126 Santa Cruz Biotechnology cyclin B1 cyclin B1 (H-433) Rabbit pAb sc-752 Santa Cruz Biotechnology cyclin D1 cyclin D1 (A-12) Mouse mAb sc-8396 Santa Cruz Biotechnology p-Histone H3 phospho-Histone H3 (Ser 10) Rabbit pAb sc-8656-R Santa Cruz Biotechnology

Mcl-1 Mcl-1 Antibody (S-19) Rabbit pAb sc-819 Santa Cruz Biotechnology

CHOP GADD 153 (R-20) Rabbit pAb sc-793 Santa Cruz Biotechnology

ATG5 Atg5 (D5F5U) Rabbit mAb #12994 Cell signaling technology p-p70 S6 Kinase Phospho-p70 S6 Kinase #9205 Cell signaling technology

(Thr389) Rabbit pAb p70 S6 Kinase p70 S6 Kinase Rabbit pAb #9202 Cell signaling technology

AMPKα AMPKα Rabbit pAb #2532 Cell signaling technology p-AMPKα Phospho-AMPKα (Thr172) Rabbit pAb #2531 Cell signaling technology

ULK1 ULK1 (D8H5) Rabbit mAb #8054 Cell signaling technology p-ULK1 Phospho-ULK1 (Ser757) Rabbit pAb #6888 Cell signaling technology p-Histone H2A.X Phospho-Histone H2A.X (Ser139) Rabbit pAb #2577 Cell signaling technology p-Histone H3 Phospho-Histone H3 (Ser10) Rabbit pAb #9708 Cell signaling technology

(Alexa Fluor® 488 Conjugate)

Cleaved PARP Cleaved PARP (Asp214) Rabbit pAb #9541 Cell signaling technology p21 p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 Cell signaling technology p-eIF2α Phospho-eIF2alpha (Ser51) Rabbit pAb #9721 Cell signaling technology eIF2α eIF2alpha Rabbit pAb #9722 Cell signaling technology pRb pRB (4H1) Mouse mAb #9309 Cell signaling technology p-pRb Phospho-pRb (Ser780) Rabbit pAb #9307 Cell signaling technology

BubR1 BubR1 Mouse mAb 612503 BD Biosciences

Bub1 Bub1 Rabbit pAb A300-373A Bethyl Laboratories

LC3 LC3B Rabbit pAb L7543 Sigma GAPDH GAPDH Mouse mAb CB1001 Merck Millipore

Lamin B1 Lamin B1 Rabbit pAb ab16048 Abcam

IRE1 IRE1 Rabbit pAb Ab37073 Abcam p-IRE1 Phospho-IRE1 (Ser724) Rabbit mAb ab124945 Abcam

β-Catenin β-Catenin Rabbit pAb ab16051 Abcam

53BP1 53BP1 Rabbit pAb ab36823 Abcam

RPA32 Anti-RPA32/RPA2 Mouse mAb ab2175 Abcam pRPA32 anti-Phospho RPA32 (S33) Rabbit pAb A300-246A Bethyl

IL-8 IL-8/CXCL8 Mouse mAb MAB208-100 R&D Systems

IL-1β IL-1 beta /IL-1F2 Mouse mAb MAB201-100 R&D Systems

PERK PERK (D11A8) Rabbit mAb 5683S Research Biolabs p-PERK Phospho-PERK(Thr980)(16F8) Rabbit mAb 3179S Research Biolabs

Rabbit IgG Donkey anti-Rabbit IgG-HRP NA934 GE Health care life sciences

Mouse IgG Sheep anti-Mouse IgG-HRP NA931 GE Health care life sciences

Mouse IgG Goat anti-Mouse IgG-Alexa Fluor 488 A-11001 Invitrogen

Mouse IgG Goat anti-Mouse IgG-Alexa Fluor 594 A-11005 Invitrogen

Rabbit IgG Goat anti-Rabbit IgG-Alexa Fluor 488 A-11008 Invitrogen

Rabbit IgG Goat anti-Rabbit IgG-Alexa Fluor 594 A-11012 Invitrogen

Supplementary Table S3. List of chemicals.

Chemical Concentration Catalogue number Company

Nocodazole (Noc) 100 ng/ml or 10 mpk N3000 US Biological

Paclitaxel (PTX) 150 nM or 10 mpk P1792A US Biological

Thymidine 3 mM T1895-25G Sigma

ZM447439 2 μM 2458 Tocris

Monastrol 100 μM 1305 Tocris

Bafilomycin A1 10 nM or 25 nM B1793 Sigma

Chloroquine 30 mpk C6628 Sigma

Recombinant IL-8 10 ng/ml 208-IL-010 R&D

Recombinant IL-1β 2.5 ng/ml 201-LB-005 R&D

PERK inhibitor (PERKi) 10 μM GSK2656157 Selleckchem

IRE1 inhibitor (4μ8C) 10 μM SML0949 Sigma

Tunicamycin 1 μg/ml T7765 Sigma

Supplementary Table S4. List of primers.

Gene Sequence (5' to 3') il-8 Forward: GAGTGGACCACACTGCGCCA Reverse: TCCACAACCCTCTGCACCCAGT il-6 Forward: CCAGGAGCCCAGCTATGAAC Reverse: CCCAGGGAGAAGGCAACTG bmp2 Forward: GGACATTCGGTCCTTGCGCCA Reverse: GGTCGACCTTTAGGAGACCGCAGT il-1α Forward: AGTGCTGCTGAAGGAGATGCCTGA Reverse: CCCCTGCCAAGCACACCCAGTA il-1β Forward: TGCACGCTCCGGGACTCACA Reverse: CATGGAGAACACCACTTGTTGCTCC fibronectin Forward: GAGGGGACCTGCAGCCACA Reverse: TTCGCAACCTGCGGGAAAAA icam1 Forward: GGCCCCCTACCAGCTCCAGA Reverse: GACTGGGAACAGCCCGTCCA vimentin Forward: ACTACGTCCACCCGCACCTA Reverse: CAGCGAGAAGTCCACCGAGT mmp-9 Forward: TTGACAGCGACAAGAAGTGG Reverse: GCCATTCACGTCGTCCTTA Slug Forward: GTGATTATTTCCCCGTATCTCTAT Reverse: CAATGGCATGGGGGTCTGAAAG Snail Forward: GCTGCCAATGCTCATCTGGGACTCT Reverse: TTGAAGGGCTTTCGAGCCTGGAGAT CHOP Forward: CATTGCCTTTCTCCTTCGGG Reverse: CCAGAGAAGCAGGGTCAAGA sxbp1 Forward: CTGAGTCCGAATCAGGTGCAG Reverse: ATCCATGGGGAGATGTTCTGG usxbp1 Forward: CAGCACTCAGACTACGTGCA Reverse: ATCCATGGGGAGATGTTCTGG Total xbp1 Forward: TGGCCGGGTCTGCTGAGTCCG Reverse: ATCCATGGGGAGATGTTCTGG p21 Forward: TGTGATGCGCTAATGGCG Reverse: AAGTCGAAGTTCCATCGCTCA lmnb1 Forward: CACTGGCGAAGATGTGAAGG Reverse: CCCTGCTGGTGGAAAAGTTC Gapdh Forward: GGTCGTATTGGGCGCCTGGTCACC Reverse: CACACCCATGACGAACATGGGGGC

Supplementary Table S5. Quantitative H scoring method.

0 1 2 3 Intensity of immunostaining (I) negative weak moderate Strong

0 10 20 30 40 50 60 70 80 90 100 Percentage of stained cells in each 0- 11- 21- 31- 41- 51- 61- 71- 81- 91- 0% intensity (P) 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%

H score was determined by summing the product of P (1-3) and percentage of stained cells in each intensity (0-100); Maximum = 300