Catalog # Aliquot Size

K423-911B-05 3 x 5 nmol K423-911B-20 3 x 20 nmol K423-911B-50 3 x 50 nmol

KDM3B siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # K423-911B

Lot # Z2049-50

Specificity Formulation KDM3B siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human KDM3B expression. shipped at room temperature.

Product Description Reconstitution Protocol KDM3B siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human KDM3B mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_016604. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot 5qNCA; C5orf7; JMJD1B; NET22 1x stock solutions for storage.

Related Products Storage and Stability

The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the KDM1A K421-31G lyophilized and resuspended siRNAs are stored at or KDM2A Protein K422-31G below -20oC. After resuspension, siRNA stock solutions ≥2 KDM4A Protein K424-31G µM can undergo up to 50 freeze-thaw cycles without KDM4B Protein K424-31BG significant degradation. For long-term storage, it is KDM4C Protein K424-31CG recommended that the siRNA is stored at -70oC. For most KDM5B Protein K425-31BG favorable performance, avoid repeated handling and KDM5C Protein K425-31CG multiple freeze/thaw cycles. Anti-KDM1A K421-63BR Anti-KDM1A K421-63R References Anti-KDM3A K423-63R 1. Lai F et al: cDNA cloning and genomic structure of Anti-KDM3B K423-63BR three localized to human band Anti-KDM4A K424-63R 5q31 encoding potential nuclear . Anti-KDM4B K424-63BR Genomics2000; 70:123-30. Anti-KDM6B K426-63BR 2. Hu Z et al: A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia KDM3B siRNA Set I tumor suppressor gene on chromosome band q31. siRNA duplexes targeted against three exon regions Oncogene2001; 20:6946-54. 3. Baik S-H et al: DNA profiling by array comparative Catalog Number K423-911B genomic hybridization (CGH) of peripheral blood Specific Lot Number Z2049-50 mononuclear cells (PBMC) and tumor tissue cell in Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol non-small cell lung cancer (NSCLC). Mol. Biol. Format Lyophilized powder Rep.2009; 36:1767-78 Stability 1yr at -70oC from date of shipment Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com

FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.