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Diversity of Echinostomes (Digenea: Echinostomatidae) in Their Snail Hosts at High Latitudes
Parasite 28, 59 (2021) Ó C. Pantoja et al., published by EDP Sciences, 2021 https://doi.org/10.1051/parasite/2021054 urn:lsid:zoobank.org:pub:9816A6C3-D479-4E1D-9880-2A7E1DBD2097 Available online at: www.parasite-journal.org RESEARCH ARTICLE OPEN ACCESS Diversity of echinostomes (Digenea: Echinostomatidae) in their snail hosts at high latitudes Camila Pantoja1,2, Anna Faltýnková1,* , Katie O’Dwyer3, Damien Jouet4, Karl Skírnisson5, and Olena Kudlai1,2 1 Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic 2 Institute of Ecology, Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania 3 Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, H91 T8NW, Galway, Ireland 4 BioSpecT EA7506, Faculty of Pharmacy, University of Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France 5 Laboratory of Parasitology, Institute for Experimental Pathology, Keldur, University of Iceland, IS-112 Reykjavík, Iceland Received 26 April 2021, Accepted 24 June 2021, Published online 28 July 2021 Abstract – The biodiversity of freshwater ecosystems globally still leaves much to be discovered, not least in the trematode parasite fauna they support. Echinostome trematode parasites have complex, multiple-host life-cycles, often involving migratory bird definitive hosts, thus leading to widespread distributions. Here, we examined the echinostome diversity in freshwater ecosystems at high latitude locations in Iceland, Finland, Ireland and Alaska (USA). We report 14 echinostome species identified morphologically and molecularly from analyses of nad1 and 28S rDNA sequence data. We found echinostomes parasitising snails of 11 species from the families Lymnaeidae, Planorbidae, Physidae and Valvatidae. -
Revealing the Secret Lives of Cryptic Species: Examining the Phylogenetic Relationships of Echinostome Parasites in North America
ARTICLE IN PRESS Molecular Phylogenetics and Evolution xxx (2010) xxx–xxx Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Revealing the secret lives of cryptic species: Examining the phylogenetic relationships of echinostome parasites in North America Jillian T. Detwiler *, David H. Bos, Dennis J. Minchella Purdue University, Biological Sciences, Lilly Hall, 915 W State St, West Lafayette, IN 47907, USA article info abstract Article history: The recognition of cryptic parasite species has implications for evolutionary and population-based stud- Received 10 August 2009 ies of wildlife and human disease. Echinostome trematodes are a widely distributed, species-rich group of Revised 3 January 2010 internal parasites that infect a wide array of hosts and are agents of disease in amphibians, mammals, and Accepted 5 January 2010 birds. We utilize genetic markers to understand patterns of morphology, host use, and geographic distri- Available online xxxx bution among several species groups. Parasites from >150 infected host snails (Lymnaea elodes, Helisoma trivolvis and Biomphalaria glabrata) were sequenced at two mitochondrial genes (ND1 and CO1) and one Keywords: nuclear gene (ITS) to determine whether cryptic species were present at five sites in North and South Cryptic species America. Phylogenetic and network analysis demonstrated the presence of five cryptic Echinostoma lin- Echinostomes Host specificity eages, one Hypoderaeum lineage, and three Echinoparyphium lineages. Cryptic life history patterns were Molecular phylogeny observed in two species groups, Echinostoma revolutum and Echinostoma robustum, which utilized both Parasites lymnaied and planorbid snail species as first intermediate hosts. Molecular evidence confirms that two Trematodes species, E. -
Melanoides Tuberculata), Species Habitat Associations and Life History Investigations in the San Solomon Spring Complex, Texas
FINAL REPORT As Required by THE ENDANGERED SPECIES PROGRAM TEXAS Grant No. TX E-121-R Endangered and Threatened Species Conservation Native springsnails and the invasive red-rim melania snail (Melanoides tuberculata), species habitat associations and life history investigations in the San Solomon Spring complex, Texas Prepared by: David Rogowski Carter Smith Executive Director Clayton Wolf Director, Wildlife 3 October 2012 FINAL REPORT STATE: ____Texas_______________ GRANT NUMBER: ___ TX E-121-R___ GRANT TITLE: Native springsnails and the invasive red-rim melania snail (Melanoides tuberculata), species habitat associations and life history investigations in the San Solomon Spring complex, Texas. REPORTING PERIOD: ____17 Sep 09 to 31 May 12_ OBJECTIVE(S): To determine patterns of abundance, distribution, and habitat use of the Phantom Cave snail (Cochliopa texana), Phantom Spring tryonia (Tryonia cheatumi), and the invasive red-rim melania snail (Melanoides tuberculta) in San Solomon Springs, and potential interactions. Segment Objectives: Task 1. January - February 2010. A reconnaissance visit(s) will be made to the region to investigate the study area and work on specific sampling procedural methods. Visit with TPWD at the Balmorhea State Park, as well as meet The Nature Conservancy personnel at Diamond Y and Sandia springs complexes. Task 2. March 2010– August 2011. Begin sampling. Field sampling will be conducted every 6-8 weeks, over a period of a year and a half. Sampling methods are outlined below stated Tasks. Task 3. December 2010. Completion of first year of study. With four seasonal samples completed, preliminary data analysis and statistical modeling will begin. Preliminary results will be presented at the Texas Chapter of the American Fisheries Society meeting. -
Trematoda: Echinostomatidae) in Thailand and Phylogenetic Relationships with Other Isolates Inferred by ITS1 Sequence
Parasitol Res (2011) 108:751–755 DOI 10.1007/s00436-010-2180-8 SHORT COMMUNICATION Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda: Echinostomatidae) in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence Weerachai Saijuntha & Chairat Tantrawatpan & Paiboon Sithithaworn & Ross H. Andrews & Trevor N. Petney Received: 2 November 2010 /Accepted: 17 November 2010 /Published online: 1 December 2010 # Springer-Verlag 2010 Abstract Echinostomatidae are common, widely distribut- an isolate from Thailand with other isolates available from ed intestinal parasites causing significant disease in both GenBank database. Interspecies differences in ITS1 se- animals and humans worldwide. In spite of their impor- quence between E. revolutum and E. recurvatum were tance, the taxonomy of these echinostomes is still contro- detected at 6 (3%) of the 203 alignment positions. Of these, versial. The taxonomic status of two species, Echinostoma nucleotide deletion at positions 25, 26, and 27, pyrimidine revolutum and Echinoparyphium recurvatum, which com- transition at 50, 189, and pyrimidine transversion at 118 monly infect poultry and other birds, as well as human, is were observed. Phylogenetic analysis revealed that E. problematical. Previous phylogenetic analyses of Southeast recurvatum from Thailand clustered as a sister taxa with Asian strains indicate that these species cluster as sister E. revolutum and not with other members of the genus taxa. In the present study, the first internal transcribed Echinoparyphium. Interestingly, this result confirms a spacer (ITS1) sequence was used for genetic characteriza- previous report based on allozyme electrophoresis and tion and to examine the phylogenetic relationships between mitochondrial DNA that E. revolutum and E. -
Infections with Digenetic Trematode Metacercariae in Freshwater Fishes from Two Visiting Sites of Migratory Birds in Gyeongsangnam-Do, Republic of Korea
ISSN (Print) 0023-4001 ISSN (Online) 1738-0006 Korean J Parasitol Vol. 57, No. 3: 273-281, June 2019 ▣ ORIGINAL ARTICLE https://doi.org/10.3347/kjp.2019.57.3.273 Infections with Digenetic Trematode Metacercariae in Freshwater Fishes from Two Visiting Sites of Migratory Birds in Gyeongsangnam-do, Republic of Korea Woon-Mok Sohn*, Byoung-Kuk Na Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea Abstract: The infection status of digenetic trematode metacercariae (DTM) was investigated in fishes from 2 representa- tive visiting sites of migratory birds in Gyeongsangnam-do, the Republic of Korea (Korea). A totaly 220 freshwater fishes (7 species) were collected from Junam-jeosuji (reservoir), and 127 fishes (7 species) were also collected from Woopo-neup (swamp) in June and October 2017. As the control group, total 312 fish (22 spp.) from Yangcheon in Sancheong-gun, Gyeongsangnam-do were also collected in June and October 2017. All fishes collected in 3 sites were examined with the artificial digestion method. In the fishes from Junam-jeosuji, more than 4 species, i.e., Clonorchis sinensis, Echinostoma spp., Diplostomum spp. and Cyathocotyle orientalis, of DTM were detected and their endemicy was very low, 0.70. More than 6 species, i.e., C. sinensis, Echinostoma spp., Metorchis orientalis, Clinostomum complanatum, Diplostomum spp. and C. orientalis, of DTM were found in the fishes from Woopo-neup, and their endemicy was low, 5.16. In the fishes from Yangcheon, more than 8 species, i.e., C. sinensis, Metagonimus spp., Centrocestus armatus, C. -
Molecular Identification of Larval Trematode in Intermediate Hosts from Chiang Mai, Thailand
SOUTHEAST ASIAN J TROP MED PUBLIC HEALTH MOLECULAR IDENTIFICATION OF LARVAL TREMATODE IN INTERMEDIATE HOSTS FROM CHIANG MAI,THAILAND Suksan Chuboon and Chalobol Wongsawad Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand Abstract. Snail and fish intermediate hosts were collected from rice fields in 3 dis- tricts; Mueang, Mae Taeng and Mae Rim of Chiang Mai Province during April-July 2008. For identification of larval trematode infection, standard (cracked for snail and enzymatically digested for fish) and molecular methods were performed. The re- sults showed that three types of cercariae were found, pleurolophocercus, cotylocercous, and echinostome among 4 species of snail with a prevalence of 29, 23 and 3% respectively. Melanoides tuberculata snail was the most susceptible host for cercariae infection. Four species of metacercariae, Haplorchis taichui, Stellantchasmus falcatus, Haplorchoides sp and Centrocestus caninus, were found with a prevalence of 67, 25, 60 and 20%, respectively. The Siamese mud carp (Henicorhynchus siamensis) was the most susceptible fish host for H. taichui, and half- beaked fish (Dermogenys pusillus) for S. falcatus metacercariae infection, whereas Haplorchoides sp and C. caninus were concomitantly found in Puntius brevis. HAT-RAPD profile confirmed that pleurolophocercus cercariae found in Melanoides tuberculata from Mae Taeng Dis- trict belonged to H. taichui and in Tarebia granifera from Mueang District were S. falcatus. INTRODUCTION among several metacercarial species in the same fish and snail hosts including their In Thailand, heterophyid flukes, morphology, which is particularly similar in Stellantchasmus falcatus, Centrocestus caninus the egg forms and larval stages, it is diffi- and Haplorchis taichui, were reported as en- cult to distinguish such parasites from one demic species in the northern region another by standard methods. -
FACTORS CONDITIONING the HABITAT of BILHARZIASIS INTERMEDIATE HOSTS of the FAMILY PLANORBIDAE EMILE ABDEL MALEK Reader in Parasitology, University of Khartoum, Sudan
Bull. Org. mond. Sante 1958, 18, 785-818 Bull. Wid Hlth Org. FACTORS CONDITIONING THE HABITAT OF BILHARZIASIS INTERMEDIATE HOSTS OF THE FAMILY PLANORBIDAE EMILE ABDEL MALEK Reader in Parasitology, University of Khartoum, Sudan SYNOPSIS In this article, the author examines certain physical, chemical and biological characteristics of water-bodies which make them suitable or unsuitable as habitats for planorbid snails acting as vectors of bilharziasis. The principal conditioning factors appear to be: amount of food available; extent of the growth of aquatic weeds; oxygen content of the water; amount of sunlight able to penetrate the water; strength of the current; natiure of the substratum; ionic composition of the water; and presence or absence of parasites and predators. Several of these factors are interdependent. Although there are differences between the various species in their habitat requirements, their ranges of tolerance were found to overlap greatly. The optimum conditions are similar for all species, but extremes are tolerated better by some species than by others. Theoretically, extremes of certain factors should be capable of eliminating snails from a body of water; in practice such extremes rarely occur, and the absence of vectors must be attributed to the combined effect of several factors. Although certain parasites and predators exterminate vectors in the laboratory, the author considers it unlikely that they would do so in nature, as under laboratory conditions the biological balance is disturbed to the disadvantage of the snail. The data available are still too scanty for an exact assessment to be made of the importance of individual environmental factors in controlling the size of vector populations; but this review of present knowledge indicates the lines along which further investigation can be most profitably pursued. -
The Complete Mitochondrial Genome of Echinostoma Miyagawai
Infection, Genetics and Evolution 75 (2019) 103961 Contents lists available at ScienceDirect Infection, Genetics and Evolution journal homepage: www.elsevier.com/locate/meegid Research paper The complete mitochondrial genome of Echinostoma miyagawai: Comparisons with closely related species and phylogenetic implications T Ye Lia, Yang-Yuan Qiua, Min-Hao Zenga, Pei-Wen Diaoa, Qiao-Cheng Changa, Yuan Gaoa, ⁎ Yan Zhanga, Chun-Ren Wanga,b, a College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China b College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China ARTICLE INFO ABSTRACT Keywords: Echinostoma miyagawai (Trematoda: Echinostomatidae) is a common parasite of poultry that also infects humans. Echinostoma miyagawai Es. miyagawai belongs to the “37 collar-spined” or “revolutum” group, which is very difficult to identify and Echinostomatidae classify based only on morphological characters. Molecular techniques can resolve this problem. The present Mitochondrial genome study, for the first time, determined, and presented the complete Es. miyagawai mitochondrial genome. A Comparative analysis comparative analysis of closely related species, and a reconstruction of Echinostomatidae phylogeny among the Phylogenetic analysis trematodes, is also presented. The Es. miyagawai mitochondrial genome is 14,416 bp in size, and contains 12 protein-coding genes (cox1–3, nad1–6, nad4L, cytb, and atp6), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and one non-coding region (NCR). All Es. miyagawai genes are transcribed in the same direction, and gene arrangement in Es. miyagawai is identical to six other Echinostomatidae and Echinochasmidae species. The complete Es. miyagawai mitochondrial genome A + T content is 65.3%, and full- length, pair-wise nucleotide sequence identity between the six species within the two families range from 64.2–84.6%. -
Complete Mitochondrial Genome of Haplorchis Taichui and Comparative Analysis with Other Trematodes
ISSN (Print) 0023-4001 ISSN (Online) 1738-0006 Korean J Parasitol Vol. 51, No. 6: 719-726, December 2013 ▣ ORIGINAL ARTICLE http://dx.doi.org/10.3347/kjp.2013.51.6.719 Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes Dongmin Lee1, Seongjun Choe1, Hansol Park1, Hyeong-Kyu Jeon1, Jong-Yil Chai2, Woon-Mok Sohn3, 4 5 6 1, Tai-Soon Yong , Duk-Young Min , Han-Jong Rim and Keeseon S. Eom * 1Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 361-763, Korea; 2Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea; 3Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-70-51, Korea; 4Department of Environmental Medical Biology, Institute of Tropical Medicine and Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul 120-752, Korea; 5Department of Immunology and Microbiology, Eulji University School of Medicine, Daejeon 301-746, Korea; 6Department of Parasitology, Korea University College of Medicine, Seoul 136-705, Korea Abstract: Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochon- drial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). -
High Prevalence of Haplorchis Taichui Metacercariae in Cyprinoid Fish from Chiang Mai Province, Thailand
H. TAICHUI METACERCARIAE IN FISH HIGH PREVALENCE OF HAPLORCHIS TAICHUI METACERCARIAE IN CYPRINOID FISH FROM CHIANG MAI PROVINCE, THAILAND Kanda Kumchoo1, Chalobol Wongsawad1, Jong-Yil Chai 2, Pramote Vanittanakom3 and Amnat Rojanapaibul1 1Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand; 2Department of Parasitology and Institute of Endemic Diseases, College of Medicine Seoul National University, Seoul, Korea; 3Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand Abstract. This study aimed to investigate Haplorchis taichui metacercarial infection in fish collected from the Chom Thong and Mae Taeng districts, Chiang Mai Province during November 2001 to October 2002. A total 617 cyprinoid fish of 15 species were randomly collected and examined for H. taichui metacercariae. All the species of fish were found to be infected with H. taichui. The infection rates were 91.4% (266/290) and 83.8% (274/327), with mean intensities of 242.9 and 107.4 in the Chom Thong and Mae Taeng districts, respectively. The portion of the fish body with the highest metacercarial density was the muscles, and second, the head, in both districts. In addition, the fish had mixed-infection with other species of trematodes, namely: Centrocestus caninus, Haplorchoides sp, and Haplorchis pumilio. INTRODUCTION port of severe pathogenicity, as is seen in the liver or lung flukes. It is known that heterophyid In Thailand, fish-borne trematode infections flukes irritate the intestinal mucosa and cause have been commonly found in the northeastern colicky pain and mucusy diarrhea, with the pro- and northern regions, including Chiang Mai Prov- duction of excess amounts of mucus and su- ince (Maning et al, 1971; Kliks and Tanta- perficial necrosis of the mucus coat (Beaver et chamrun, 1974; Pungpak et al, 1998; Radomyos al, 1984; Chai and Lee, 2002). -
High Prevalence of Clonorchis Sinensis and Other Zoonotic Trematode Metacercariae in Fish from a Local Market in Yen Bai Province, Northern Vietnam
ISSN (Print) 0023-4001 ISSN (Online) 1738-0006 Korean J Parasitol Vol. 58, No. 3: 333-338, June 2020 ▣ BRIEF COMMUNICATION https://doi.org/10.3347/kjp.2020.58.3.333 High Prevalence of Clonorchis sinensis and Other Zoonotic Trematode Metacercariae in Fish from a Local Market in Yen Bai Province, Northern Vietnam 1,6 1 2 3 4 5 5, Fuhong Dai , Sung-Jong Hong , Jhang Ho Pak , Thanh Hoa Le , Seung-Ho Choi , Byoung-Kuk Na , Woon-Mok Sohn * 1Department of Environmental Medical Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea; 2Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea; 3Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam; 4Society of Korean Naturalist, Institute of Ecology and Conservation, Yangpyeong 12563, Korea; 5Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea, 6Department of Parasitology, School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu 215123, P.R. China Abstract: A small survey was performed to investigate the recent infection status of Clonorchis sinensis and other zoo- notic trematode metacercariae in freshwater fish from a local market of Yen Bai city, Yen Bai province, northern Vietnam. A total of 118 fish in 7 species were examined by the artificial digestion method on March 2016. The metacercariae of 4 species of zoonotic trematodes, i.e., C. sinensis, Haplorchis pumilio, Haplorchis taichui, and Centrocestus formosanus, were detected. The metacercariae of C. sinensis were found in 62 (69.7%) out of 89 fish (5 species), and their intensity of infection was very high, 81.2 per fish infected. -
GASTROPODA: THIARIDAE) EN MEDELLÍN, COLOMBIA Acta Biológica Colombiana, Vol
Acta Biológica Colombiana ISSN: 0120-548X [email protected] Universidad Nacional de Colombia Sede Bogotá Colombia VERGARA, DANIELA; VELÁSQUEZ, LUZ ELENA LARVAS DE DIGENEA EN Melanoides tuberculata (GASTROPODA: THIARIDAE) EN MEDELLÍN, COLOMBIA Acta Biológica Colombiana, vol. 14, núm. 1, 2009, pp. 135-142 Universidad Nacional de Colombia Sede Bogotá Bogotá, Colombia Disponible en: http://www.redalyc.org/articulo.oa?id=319027882008 Cómo citar el artículo Número completo Sistema de Información Científica Más información del artículo Red de Revistas Científicas de América Latina, el Caribe, España y Portugal Página de la revista en redalyc.org Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto Acta biol. Colomb., Vol. 14 No. 1, 2009 135 - 142 LARVAS DE DIGENEA EN Melanoides tuberculata (GASTROPODA: THIARIDAE) EN MEDELLÍN, COLOMBIA Larval stages of digenea from Melanoides tuberculata (Gastropoda: Thiaridae) in Medellín, Colombia DANIELA VERGARA1, Microbióloga, Estudiante Ph. D.; LUZ ELENA VELÁSQUEZ1,2, Bióloga M.Sc. 1 Programa de Estudio y Control de Enfermedades Tropicales PECET. Sede de Investigación Universitaria SIU Universidad de Antioquia. Calle 62 No. 52-69. 2 Escuela de Microbiología/UdeA Correspondencia: Luz Elena Velásquez. [email protected] Sede de Investigación Universitaria SIU Universidad de Antioquia. Calle 62 No. 52-69, Torre 2, laboratorio 730. Teléfono: 219 65 14. Fax 219 65 11. Medellín, Colombia. Presentado 14 de agosto de 2008, aceptado 20 de octubre de 2008, correcciones 10 de diciembre de 2008. RESUMEN Se describen las larvas de digeneos que se obtuvieron en Melanoides tuberculata (Gastropoda: Thiaridae), molusco dulceacuícola del que se colectaron 125 especíme- nes en el lago del Jardín Botánico Joaquín Antonio Uribe de Medellín.