CD3G Antibody Cat. No.: 58-129
CD3G Antibody
Formalin-fixed and paraffin-embedded human brain Flow cytometric analysis of HL-60 cells using CD3G tissue reacted with CD3G antibody , which was Antibody (bottom histogram) compared to a negative peroxidase-conjugated to the secondary antibody, control cell (top histogram). FITC-conjugated goat-anti- followed by DAB staining. rabbit secondary antibodies were used for the analysis.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
This CD3G antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 48-76 amino acids from the N-terminal region of human CD3G.
TESTED APPLICATIONS: Flow, IHC-P, WB
For WB starting dilution is: 1:1000
APPLICATIONS: For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
October 2, 2021 1 https://www.prosci-inc.com/cd3g-antibody-58-129.html PREDICTED MOLECULAR 20 kDa WEIGHT:
Properties
This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: CD3G
T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD3g, ALTERNATE NAMES: CD3G, T3G
ACCESSION NO.: P09693
PROTEIN GI NO.: 115993
GENE ID: 917
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
CD3G is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen BACKGROUND: recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in the CD3G gene are associated with T cell immunodeficiency.
REFERENCES: 1) Siegers,G.M., J. Exp. Med. 204 (11), 2537-2544 (2007)
2) Sigalov,A.B., Biochemistry 45 (51), 15731-15739 (2006)
October 2, 2021 2 https://www.prosci-inc.com/cd3g-antibody-58-129.html ANTIBODIES FOR RESEARCH USE ONLY.
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