CD3G Antibody Cat. No.: 58-129

CD3G Antibody

Formalin-fixed and paraffin-embedded human brain Flow cytometric analysis of HL-60 cells using CD3G tissue reacted with CD3G antibody , which was Antibody (bottom histogram) compared to a negative peroxidase-conjugated to the secondary antibody, control cell (top histogram). FITC-conjugated goat-anti- followed by DAB staining. rabbit secondary antibodies were used for the analysis.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

This CD3G antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 48-76 amino acids from the N-terminal region of human CD3G.

TESTED APPLICATIONS: Flow, IHC-P, WB

For WB starting dilution is: 1:1000

APPLICATIONS: For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

October 2, 2021 1 https://www.prosci-inc.com/cd3g-antibody-58-129.html PREDICTED MOLECULAR 20 kDa WEIGHT:

Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: CD3G

T-cell surface glycoprotein CD3 gamma chain, T-cell T3 gamma chain, CD3g, ALTERNATE NAMES: CD3G, T3G

ACCESSION NO.: P09693

PROTEIN GI NO.: 115993

GENE ID: 917

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

CD3G is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen BACKGROUND: recognition to several intracellular signal-transduction pathways. The encoding the epsilon, gamma and delta polypeptides are located in the same cluster on 11. Defects in the CD3G are associated with T cell immunodeficiency.

REFERENCES: 1) Siegers,G.M., J. Exp. Med. 204 (11), 2537-2544 (2007)

2) Sigalov,A.B., Biochemistry 45 (51), 15731-15739 (2006)

October 2, 2021 2 https://www.prosci-inc.com/cd3g-antibody-58-129.html ANTIBODIES FOR RESEARCH USE ONLY.

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