Apenidiella Antarctica Fungal Planet Description Sheets 421

420 Persoonia – Volume 43, 2019

Apenidiella antarctica Fungal Planet description sheets 421

Fungal Planet 1041 – 18 December 2019 Apenidiella antarctica Ivanushkina, Kochkina, Vasilenko & Ozerskaya, sp. nov.

Etymology. Named after Antarctica, where the fungus was collected. Typus. Antarctica, Russkaya Station (S74°45'48" W136°47'47", altitude 76 m), hole A8/08, depth 1.3–1.4 m, isolated from permafrost, N. Ivanush- Classiﬁcation — Teratosphaeriaceae, Capnodiales, Dothi kina (holotype VKM H-0001, ex-type culture VKM F-4540, SSU/ITS/LSU deomycetidae, Dothideomycetes. sequence GenBank MK770828.1, MycoBank MB830584). Mycelium consisting of branched, septate, smooth to warty, Notes — Apenidiella antarctica is the second member of the hyaline to pale olivaceous, 1–4 μm wide hyphae. Conidiophores genus Apenidiella (Crous et al. 2007a, Quaedvlieg et al. 2014). solitary, erect, arising from superﬁcial mycelium, macronema- Based on a megablast search of NCBIs GenBank nucleotide tous, subcylindrical, straight to slightly curved, subcylindrical database, the closest hits using the ITS sequence are Apeni throughout, 30–100 × 2.5–4.5 μm, 0–6-septate, medium to diella strumelloidea (GenBank NR_145090.1; Identities = 547/ dark brown, paler towards the apex, smooth, wall ≤ 0.75 μm 564 (97 %), 2 gaps (0 %)), Cercosporella dolichandrae (Gen- diam, penicillate apex formed by a terminal conidiogenous cell Bank NR_156282.1; Identities = 489/574 (85 %), 32 gaps giving rise to a single set of ramoconidia. Conidiogenous cells (5 %)), Hortaea thailandica (GenBank GU214637.1; Iden- terminal, rarely intercalary integrated, subcylindrical, straight to tities = 491/578 (85 %), 26 gaps (4 %)). Closest hits using curved, 8–30 × 2.5–4.5 μm, pale brown, thin-walled, smooth, the partial LSU sequence are Apenidiella strumelloidea (Gen- with several (–10) terminal and intercalary conidiogenous loci, Bank EU019277.1; Identities = 800/805 (99 %), no gaps), thickened and darkened, scars protuberant, 1–1.5 μm diam. Microcyclospora tardicrescens (GenBank MH875507.1; Identi- Conidia in short (–5), dense penicillate, acropetal chains, ties = 778/806 (97 %), 2 gaps (0 %)), M. pomicola (GenBank ramoconidia subcylindrical, with 1–3 terminal loci, olivaceous MH875506.1 with the same statistics), and Microcyclospora brown, smooth, 11–12.5 × 3.5–4.5(–5) μm; secondary conidia malicola (GenBank MH875503.1; Identities = 775/806 (96 %), ellipsoid to obovoid, (7–)8.5–10(–11) × (3.5–)4–5.5(–6) μm, 2 gaps (0 %)). Closest hits using the contiguous tandem ITS hila not thickened or almost so to somewhat thickened and plus LSU (including D1-D3 domains) sequence are Apenidiella darkened, not refractive, 1 μm diam. strumelloidea (GenBank EU019277.1; Identities = 1345/1367 Culture characteristics — (in the dark, PDA, 25 °C after 1 mo). (98 %), 2 gaps (0 %)), Eupenidiella venezuelensis (GenBank Colonies olivaceous grey, dense, aerial mycelium abundant, EU019277.1; Identities = 1228 /1351 (91 %), 29 gaps (2 %)), felty to woolly, growth regular, low convex with an elevated col- Teratoramularia kirschneriana (GenBank GU214669.1; Identi- ony centre, sometimes forming few large prominent exudates, ties = 1210/1343 (90 %), 26 gaps (1 %)). Apenidiella antarctica reverse iron-grey, margin almost colourless, regular, colonies differs morphologically from A. strumelloidea VKM F-2534T fertile; colonies reaching 20–22 mm diam (at 25 °C), 26–29 (= CBS 114484T) in having numerous loci aggregated or spread mm diam (at 20 °C), 20–21 mm diam (at 15 °C), 2–3 mm over the whole conidiogenous cell, short and little branched diam (at 5 °C), no growth (at 30 °C). conidial chains, and wider, not curved conidia.

Verrucocladosporium dirinae CBS 112794 EU040244.1 100 Toxicocladosporium irritans CBS 185.58 EU040243.2

Cladosporium ossifragi CBS 842.91 EF679381.2 85 Cladosporium iridis CBS 138.40 EU167591.1 100 Cladosporium antarcticum CBS 690.92 EF679334.2

85 Ramularia acroptili CBS 120252 GU214689.1

100 Ramularia stellariicola CPC 11297 GU214693.1 26 87 Ramularia proteae CBS 112161 EU707899.2

Xenoramularia polygonicola CPC 10852 GU214695.1 24 100 Zymoseptoria tritici CBS 100335 EU019297.2

77 Mycosphaerella stromatosa CBS 101953 EU167598.2 56 Exutisphaerella laricina CBS 326.52 EU167595.1

Pseudocercospora cercidicola CBS 132041 MH878238.1

99 91 Cercospora beticola CPC 11557 AY840527.2 Zasmidium cerophilum CBS 103.59 AF050286.1

77 Epicoleosporium ramularioides CPC 10672 GU214688.1 Teratoramularia kirschneriana CBS 113093 GU214669.1 29 Staninwardia suttonii CPC 13055 DQ923535.2

Apenidiella antarctica VKM F-4540 MK770828.1 44 100 Apenidiella strumelloidea CBS 114484 EU019277.1 43 Batcheloromyces proteae CBS 110696 JF746163.1

76 Stenella araguata CBS 105.75 EU019250.2 88 Eupenidiella venezuelensis CBS 106.75 EU019278.1

70 Neocatenulostroma microsporum CBS 101951 EU167572.1

69 Constantinomyces oldenburgensis T2.1 LT976552.1 Maximum likelihood phylogenetic tree inferred from ITS+LSU sequences Saccharata capensis CBS 122693 EU552130.1 performed with MEGA-Х (Kumar et al. 2018) using the Kimura 2-parameter model. Bootstrap support values (500 replicates) are shown at the nodes. The new species described in this study is marked with the black circle, where as 0.05 white ones mark the known members of Teratosphaeriaceae, white squares Colour illustrations. David Gilichinsky at the Russkaya Station in Marie mark Mycosphaerellaceae and triangles mark Cladosporiaceae. Saccharata Byrd Land, Antarctica, busy sampling via the dry drilling technique. Colonies capensis represents the outgroup. The alignment and tree are deposited in on PDA; conidiophores and conidiogenous cells; conidia. Scale bars = 10 µm. TreeBASE (S24413).

Nataliya E. Ivanushkina, Galina A. Kochkina, Oleg V. Vasilenko & Svetlana M. Ozerskaya, All-Russian collection of microorganisms (VKM), IBPM RAS, prospect Nauki, 5, Pushchino, Moscow Region, Russia; e-mail: [email protected], [email protected], [email protected] & [email protected]