Product Datasheet

DFFB Antibody

Catalog No: #36776

Orders: [email protected] Description Support: [email protected]

Product Name DFFB Antibody

Host Species Rabbit

Clonality Polyclonal

Purification Antigen affinity purification.

Applications IHC

Species Reactivity Hu

Specificity The antibody detects endogenous levels of total DFFB .

Immunogen Type Peptide

Immunogen Description Synthetic peptide corresponding to a region derived from internal residues of human DNA fragmentation

factor, 40kDa, beta polypeptide (caspase-activated DNase)

Target Name DFFB

Other Names CAD; CPAN; DFF2; DFF40; DFF-40

Accession No. Swiss-Prot#: O76075NCBI ID: 1677Gene Accssion: NP_004393.1

Concentration 1mg/ml

Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Storage Store at -20°C

Application Details

Immunohistochemistry: 1:25-1:100

Images

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #36776 at dilution 1/30.

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during . DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript

Address: 8400 Baltimore Ave. Suite 302 College Park MD 20740 USA http://www.sabbiotech.com 1 variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined.

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave. Suite 302 College Park MD 20740 USA http://www.sabbiotech.com 2