Anti-ADAM22 Antibody [S57-2] (ARG22288)

ARG22288 Package: 50 μg anti-ADAM22 antibody [S57-2] Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody [S57-2] recognizes ADAM22

Tested Reactivity Hu, Ms, Rat

Tested Application ICC/IF, IP, WB

Specificity Detects the extracellular domain of ADAM22 ~90kDa. Does not cross-react with ADAM11. Weakly reactive in human samples.

Host Mouse

Clonality Monoclonal

Clone S57-2

Isotype IgG1

Target Name ADAM22

Antigen Species Mouse

Immunogen Fusion protein amino acids 444-526 (extracellular disintegrin domain) of Mouse ADAM22

Conjugation Un-conjugated

Alternate Names MDC2; Disintegrin and metalloproteinase domain-containing protein 22; ADAM 22; Metalloproteinase- like, disintegrin-like, and cysteine-rich protein 2; Metalloproteinase-disintegrin ADAM22-3

Application Instructions

Application table Application Dilution

ICC/IF Assay-dependent

IP Assay-dependent

WB 1:1000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw ~90 kDa

Properties

Form Liquid

Purification Purification with Protein G.

Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol

Preservative 0.09% Sodium azide

Stabilizer 50% Glycerol

www.arigobio.com 1/3 Concentration 1 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links GeneID: 11496 Mouse

GeneID: 53616 Human

Swiss-port # Q9P0K1 Human

Swiss-port # Q9R1V6 Mouse

Gene Symbol Adam22

Gene Full Name a disintegrin and metallopeptidase domain 22

Background This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell- matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]

Function Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. [UniProt]

Cellular Localization Membrane

Images

ARG22288 anti-ADAM22 antibody [S57-2] ICC/IF image

Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22288 anti-ADAM22 antibody [S57-2] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22288 anti-ADAM22 antibody [S57-2] (D) Composite.

www.arigobio.com 2/3 ARG22288 anti-ADAM22 antibody [S57-2] WB image

Western blot: 1) MW ladder, 20 µg of Rat brain lysates stained with ARG22288 anti-ADAM22 antibody [S57-2] (60 min at RT) at 2) 1:250, 3) 1:500, and 4) 1:1000 dilution. Block: 5% milk + TBST O/N at 4°C.