PRPF8 Antibody Cat. No.: 19-988

PRPF8 Antibody

Immunohistochemistry of paraffin-embedded human Immunohistochemistry of paraffin-embedded rat brain esophagus using PRPF8 antibody (19-988) at dilution of using PRPF8 antibody (19-988) at dilution of 1:100 (40x 1:100 (40x lens). lens).

Immunohistochemistry of paraffin-embedded mouse Immunofluorescence analysis of U-251MG cells using stomach using PRPF8 antibody (19-988) at dilution of 1:100 PRPF8 antibody (19-988) at dilution of 1:100 (20x lens). (40x lens). Blue: DAPI for nuclear staining.

Specifications

HOST SPECIES: Rabbit

September 29, 2021 1 https://www.prosci-inc.com/prpf8-antibody-19-988.html SPECIES REACTIVITY: Human, Mouse, Rat

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IMMUNOGEN: PRPF8 (NP_006436.3).

TESTED APPLICATIONS: IF, IHC, IP, WB

WB: ,1:500 - 1:1000

IHC: ,1:50 - 1:100 APPLICATIONS: IF: ,1:50 - 1:100

IP: ,1:50 - 1:100

POSITIVE CONTROL: 1) HeLa

2) Jurkat

PREDICTED MOLECULAR Observed: 274kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: PRPF8

HPRP8, , PRPC8, RP13, SNRNP22pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific , PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 ALTERNATE NAMES: snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p22precursor mRNA processing protein, splicing factor Prp8

GENE ID: 10594

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 29, 2021 2 https://www.prosci-inc.com/prpf8-antibody-19-988.html Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this is a component of both U2- and U12-dependent , and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD BACKGROUND: repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant .

ANTIBODIES FOR RESEARCH USE ONLY.

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