PRPF8 Antibody Cat. No.: 19-988
PRPF8 Antibody
Immunohistochemistry of paraffin-embedded human Immunohistochemistry of paraffin-embedded rat brain esophagus using PRPF8 antibody (19-988) at dilution of using PRPF8 antibody (19-988) at dilution of 1:100 (40x 1:100 (40x lens). lens).
Immunohistochemistry of paraffin-embedded mouse Immunofluorescence analysis of U-251MG cells using stomach using PRPF8 antibody (19-988) at dilution of 1:100 PRPF8 antibody (19-988) at dilution of 1:100 (20x lens). (40x lens). Blue: DAPI for nuclear staining.
Specifications
HOST SPECIES: Rabbit
September 29, 2021 1 https://www.prosci-inc.com/prpf8-antibody-19-988.html SPECIES REACTIVITY: Human, Mouse, Rat
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IMMUNOGEN: PRPF8 (NP_006436.3).
TESTED APPLICATIONS: IF, IHC, IP, WB
WB: ,1:500 - 1:1000
IHC: ,1:50 - 1:100 APPLICATIONS: IF: ,1:50 - 1:100
IP: ,1:50 - 1:100
POSITIVE CONTROL: 1) HeLa
2) Jurkat
PREDICTED MOLECULAR Observed: 274kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: PRPF8
HPRP8, PRP8, PRPC8, RP13, SNRNP22pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 ALTERNATE NAMES: snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p22precursor mRNA processing protein, splicing factor Prp8
GENE ID: 10594
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
September 29, 2021 2 https://www.prosci-inc.com/prpf8-antibody-19-988.html Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD BACKGROUND: repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
ANTIBODIES FOR RESEARCH USE ONLY.
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