Human HIST3H2A / H2A

Catalog Number: 10926-HNAE

General Information SDS-PAGE:

Gene Name Synonym:

MGC3165

Protein Construction:

A DNA sequence encoding the human HIST3H2A (NP_254280.1) (Met 1- Lys 130) was expressed and purified.

Source: Human Expression Host: E. coli QC Testing

Purity: > 90 % as determined by SDS-PAGE Endotoxin: Protein Description Please contact us for more information. are a complex family of highly conserved basic Stability: responsible for packaging chromosomal DNA into . There are subtype diversities: H1, H2A, H2B and H3 or H4. It has become more and ℃ Samples are stable for up to twelve months from date of receipt at -70 more evident that histone modifications are key players in the regulation of states and dynamics as well as in expression. Therefore, Met 1 Predicted N terminal: histone modifications and the enzymatic machineries that set them are Molecular Mass: crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a The recombinant human HIST3H2A consists of 130 amino acids and has a double-edged sword because they also damage host tissue and may calculated molecular mass of 14.2 kDa as estimated in SDS-PAGE under cause death. Histones bound to platelets, induced calcium influx, and reducing conditions. recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone cluster 3, H2a also known as histone H2A Formulation: (HIST3H2A) is a member of histones. Covalent modification of histones is important in regulating chromatin dynamics and transcription. One example Lyophilized from sterile 20mM β-Mercaptoethanol, pH 6.9 of such modification is ubiquitination, which mainly occurs on histones H2A and H2B. E3 ubiquitin ligase complex is specific for histone H2A Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as (HIST3H2A). Reducing the expression of Ring2 results in a dramatic protectants before lyophilization. Specific concentrations are included in decrease in the level of ubiquitinated H2A in HeLa cells. DNA damage the hardcopy of COA. Please contact us for any concerns or special induces monoubiquitylation of histone H2A (HIST3H2A) in the vicinity of requirements. DNA lesions.

Usage Guide References Storage: 1.Fuchs TA, et al. (2011) Histones induce rapid and profound thrombocytopenia in mice. Blood. 118(13): 3708-14. 2.Collart D, et al. Store it under sterile conditions at -20℃ to -80℃ upon receiving. (1993) A human histone H2B.1 variant gene, located on 1, Recommend to aliquot the protein into smaller quantities for optimal utilizes alternative 3' end processing. J Cell Biochem. 50 (4): 374-85. storage. 3.Marzluff WF, et al. (2002) The human and mouse replication-dependent histone . Genomics. 80 (5): 487-98. Avoid repeated freeze-thaw cycles.

Reconstitution:

Detailed reconstitution instructions are sent along with the products.

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