MXI1 Antibody Cat. No.: 27-335

MXI1 Antibody

Antibody used in WB on Human brain at 0.2-1 ug/ml. Antibody used in IHC on Human Lung at 5 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Dog, Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human MXI1.

TESTED APPLICATIONS: ELISA, WB

MXI1 antibody can be used for detection of MXI1 by ELISA at 1:312500. MXI1 antibody can APPLICATIONS: be used for detection of MXI1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 26 kDa, 21 kDa, 33 kDa WEIGHT:

September 29, 2021 1 https://www.prosci-inc.com/mxi1-antibody-27-335.html Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store MXI1 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: MXI1

ALTERNATE NAMES: MXI1, MAD2, MGC43220, MXD2, MXI, bHLHc11

ACCESSION NO.: NP_005953

PROTEIN GI NO.: 57242782

GENE ID: 4601

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcriptional repressor protein thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in MXI1 are frequently found in patients with prostate tumors.Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in BACKGROUND: human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.

REFERENCES: 1) Zhang, K., (2005) Biochem. Biophys. Res. Commun. 336 (1), 274-280.

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