Product datasheet Anti-API5 Antibody Catalog Number: BA1768

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-API5 Antibody Name API5 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,IHC-F,ICC Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human inhibitor 5(487-504aa KYSSNLGNFNYERSLQGK)(b), different from the related mouse sequence by one amino acid. Purification Immunogen affinity purified. Observed MW 58KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 Immunohistochemistry(Frozen Section): 1:50-400 Immunocytochemistry: 1:50-400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5(API5) is an antiapoptotic factor which may have a role in assembly. The API5 gene to 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F- dependent apoptosis without generally blocking E2F-dependent transcription.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-API5 Antibody Catalog Number: BA1768

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Lane 1: Rat Cardiac IHC(P): Rat Brain Tissue Muscle Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Testis Tissue LysateLane 4: Rat Placenta Tissue LysateLane 5: MCF-7 Cell LysateLane 6: HELA Cell LysateLane 7: CEM Cell LysateLane 8: SMMC Cell LysateLane 9: COLO320 Cell Lysate

ICC: HELA Cell IHC(F): Rat Cardiac Muscle Tissue

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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