NXF1 Antibody Cat. No.: 29-372

NXF1 Antibody

Antibody used in WB on Human HepG2 at 1.25 ug/ml. Antibody used in WB on Human HeLa at: 1:300 (Lane 1: 7ug HeLa lysate+EGFP SiRNA , Lane 2: 7ug HeLa lysate+NXF1 SiRNA).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human NXF1.

TESTED APPLICATIONS: IHC, WB

NXF1 antibody can be used for detection of NXF1 by ELISA at 1:312500. NXF1 antibody can APPLICATIONS: be used for detection of NXF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate

PREDICTED MOLECULAR 68 kDa, 40 kDa WEIGHT:

September 25, 2021 1 https://www.prosci-inc.com/nxf1-antibody-29-372.html Properties

PURIFICATION: Antibody is purified by A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store NXF1 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: NXF1

ALTERNATE NAMES: NXF1, TAP, MEX67

ACCESSION NO.: NP_006353

PROTEIN GI NO.: 15487670

GENE ID: 10482

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

NXF1 is one member of a family of nuclear RNA export factor. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a -associated domain that mediates interactions with . The LRRs and NTF2-like domains are required for export activity. NXF1 shuttles between the nucleus and the cytoplasm and binds in vivo to poly (A)+ RNA. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D .This is one member of a family of nuclear RNA export factor . BACKGROUND: Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly (A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.

September 25, 2021 2 https://www.prosci-inc.com/nxf1-antibody-29-372.html REFERENCES: 1) Chen, I.H., (2005) J. Virol. 79 (7), 3949-3961.

ANTIBODIES FOR RESEARCH USE ONLY.

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